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I would like to find a detailed protocol explaining how to test the migration of murine hematopoietic stem cells through an endothelial cell layer isolated from mouse bone marrow. The idea is to use a transwell system where the endothelial cells will be cultured on the transwell and the stem cells will be attracted by SDF1 in the lower chamber. How should we isolate the endothelial cells and culture them? How should we perform the assay? Thank you very much for your help.