Catarina Cruzeiro

Catarina Cruzeiro
Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) | HZM · Research Unit Comparative Microbiome Analysis

PhD

About

58
Publications
12,129
Reads
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1,116
Citations
Additional affiliations
May 2019 - November 2020
Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH)
Position
  • PostDoc Position
Description
  • Catarina Cruzeiro joined the group in 2019 as a postdoctoral fellow, funded by the European project IDOUM (Water challenges for a changing world – IC4Water) as part of the WATER21015 JPI. Her work is focused on the removal of antibiotics from wastewater, by using selected macrophytes and microorganisms (as fungi, endophytic bacteria and macroalgae), in a constructed wetland scenario. https://www.helmholtz-muenchen.de/comi/research/research-group-plant-microbiome/team/index.html
March 2017 - November 2020
University of Coimbra
Position
  • PostDoc Position
January 2012 - June 2016
University of Porto
Position
  • PhD Student
Education
January 2012 - June 2016
University of Porto
Field of study
  • Biomedical Sciences
July 2007 - May 2009
Universidade de Trás-os-Montes e Alto Douro
Field of study
  • Zootechnic Engineer
September 2002 - June 2007
Universidade de Trás-os-Montes e Alto Douro
Field of study
  • Zootechnic Engineer

Publications

Publications (58)
Cover Page
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Explore Our Exciting Special Issue! Dive into the latest research with our special issue! We're covering a range of captivating topics, including: - Cutting-edge technology development and validation - The profound economic and social impact of our innovations - Bioremediation strategies that make a difference - In-depth exploration of metabolic...
Article
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Synthetic progestins are micropollutants of special concern, due to their growing use in human and veterinary therapies and their risks to aquatic life. Currently, there is a lack of environmental information on these compounds, worldwide. The main objective of this work was to characterize the levels of the most consumed progestins in Portugal. Fo...
Article
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The intensive use of antibiotics (for human, veterinary, and agricultural purposes) has steadily increased over the last 30 years. Large amounts of antibiotic residues are released into aquatic systems, mostly due to inefficient wastewater treatment. Conventional wastewater treatments are not designed to remove emerging contaminants (such as antibi...
Article
Insufficiently treated reclaimed water can act as a source of contamination by introducing recalcitrant contaminants (e.g., pharmaceutical compounds) to various water bodies and/or agricultural soils after irrigation. Tramadol (TRD) is one of these pharmaceuticals that can be detected in influents and effluents of wastewater treatment plants, at di...
Article
Coastal areas are affected by multiple stressors like climate change and endocrine disruptors (EDCs). In the laboratory, we investigated the combined effects of increased temperature and EDCs (drospirenone and mercury) on the fitness and gonads' maturation dynamics of the marine gastropod Nucella lapillus for 21 days. Survival was negatively affect...
Article
Full-text available
The extraction of 21 insecticides and 5 metabolites was performed using an optimized and validated QuEChERS protocol that was further used for the quantification (GC–MS/MS) in several seafood matrices (crustaceans, bivalves, and fish-mudskippers). Seven species, acquired from Hong Kong and Macao wet markets (a region so far poorly monitored), were...
Cover Page
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https://www.mdpi.com/journal/toxics/special_issues/T829GRO7GT
Article
The composition of root exudates is modulated by several environmental factors, and it remains unclear how that affects beneficial rhizosphere or inoculated microorganisms under heavy metal (HM) contamination. Therefore, we evaluated the transcriptional response of Pseudomonas putida E36 (a Miscanthus x giganteus isolate with plant growth promotion...
Article
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Metabolomics approaches provide a vast array of analytical datasets, which require a comprehensive analytical, statistical, and biochemical workflow to reveal changes in metabolic profiles. The biological interpretation of mass spectrometric metabolomics results is still obstructed by the reliable identification of the metabolites as well as annota...
Poster
Full-text available
Untargeted screening workflow of Lemna minor metabolomes changes caused by carbamazepine Metabolomics is the approach that deals with the investigation of a biological system (cell, tissue, or organism) by determining its overall metabolite profile at a given time point with the specified set of conditions. It can be targeted, suspected, or untarge...
Poster
Untargeted screening workflow of Lemna minor metabolomes changes caused by carbamazepine Metabolomics is the approach that deals with the investigation of a biological system (cell, tissue, or organism) by determining its overall metabolite profile at a given time point with the specified set of conditions. It can be targeted, suspected, or untarge...
Article
Genotoxic effects of dicofol on the edible clam Meretrix meretrix were investigated through a mesocosm experiment. Individuals of M. meretrix, were exposed to environmental concentration (D1 = 50 ng/L) and supra-environmental concentration (D2 = 500 ng/L) of dicofol for 15 days, followed by the same depuration period. DNA damage (i.e., strand break...
Article
Tramadol (TRD) is widely detected in aquatic ecosystems as a result of massive abuse and insufficient removal from wastewater facilities. As a result, TRD can contaminate groundwater sources and/or agricultural soils. While TRD toxicity has been reported on aquatic biota, data about TRD detection in plants are scarce. Moreover, information regardin...
Article
Metabolomics is an emerging approach that investigates the changes in the metabolome profile. In the present study, Lemna minor —considered as an experimental aquatic plant model— was incubated with 10 and 100 μM diclofenac (DCF) for 96 h, respectively. Knowing that DCF is internationally often problematic in wastewater effluents and that it might...
Article
Mangroves are a unique group of plants growing along tropical and sub-tropical coastlines, with the ability to remove several types of contaminants such as heavy metals and other persistent organic compounds in coastal waters. However, little attention has been given to the possible role of mangroves in the removal of organochlorinated pesticides (...
Article
The toxicity, volatility and persistence of the obsolete organochlorine pesticide hexachlorocyclohexane (HCH), makes reclamation of contaminated areas a priority for the health and welfare of neighboring human communities. Microbial diversity and functions and their relation to spontaneous vegetation in post-excavation situations, are essential ind...
Chapter
Pharmaceuticals originating from reclaimed wastewater or biosolid-, livestock manure- or sewage sludge-amended soils can enter crops by irrigation and fertilization. Generally, the putative uptake occurs through the plants’ roots and can lead to the bioaccumulation in different plant parts. The uptake and translocation therefore is dependent on mul...
Article
Uptake and depuration kinetics of 4,4′-dichlorobenzophenone (main metabolite of dicofol) in the edible clam Meretrix meretrix were evaluated through a mesocosm experiment. M. meretrix was exposed to different dicofol concentrations (environmental concentration, D1 = 50 ng/L; supra-environmental concentration, D2 = 500 ng/L) for 15 days, followed by...
Article
The Pearl River Delta (PRD) is one of the areas with higher environmental concentration of organochlorine pesticides (OCPs), being DDT one of the most abundant. In this work, 4,4′-dichlorobenzophenone (4,4′-DCBP), a common metabolite of dicofol (DDT related) and DDT, was quantified in surface waters of Hong Kong and Macao, together with the analysi...
Article
Assessing fish liver status is common in aquaculture nutrition assays. This often implies determining hepatocytes profile areas in routine thin (5–7 μm) histological sections. However, there are theoretical problems using planar morphometry in thin sections: inherent sampling cells biases, too small numbers of sampled cells, under/overestimation of...
Article
Climate change and pharmaceutical contamination are two priority research topics due to their impacts in the aquatic ecosystems and in the food chain structure. In the bottom of many food chains are the invertebrates, like the amphipods, which are important environmental and ecotoxicological models. In this study, we combined the increase of temper...
Article
As a case study, the estuary of the international Douro River (Iberian Peninsula) was sampled over a year (2010) at six sampling sites to determine the presence of 56 pesticides of different categories (insecticides, herbicides, and fungicides). 96% of measured pesticides were detected in 79% of the quantified samples. Individual average pesticide...
Article
Full-text available
This study investigated the presence of 16 priority polycyclic aromatic compounds (PAHs) in waters from the Douro River estuary and nearby Atlantic seacoast, which both bath the Porto metropolis. In the area, there is an oil refinery, an important harbour, an intense maritime traffic, small marinas and highly inhabited cities. For the analysis of P...
Article
A method for quantitative determination of 55 pesticides in a bivalve matrix was established, based on QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) extraction and using gas chromatography (GC)-ion trap (IT) mass spectrometry (MS/MS). Accomplishing the European SANCO guidelines, this method was validated using 5 g of homogenized soft t...
Article
Full-text available
The Mira River is a Portuguese water body widely known for its wilderness and is advertised as one of the less polluted European rivers. On this presumption, the levels of endocrine-disrupting compounds (EDCs) in Mira waters were never measured. However, because environmentalists have claimed that the Mira could be moderately polluted, a range of 1...
Article
The origin and diversification of the metazoan endocrine systems represents a fundamental research issue in biology. Nuclear receptors are critical components of these systems. A particular group named VDR/PXR/CAR (NR1I/J) is central in the mediation of detoxification responses. While orthologues have been thoroughly characterized in vertebrates, a...
Article
Full-text available
Ria de Aveiro is a Portuguese lagoon renowned for its ecological and economic importance. Nonetheless, in literature, few data exist about its organic pollution. Accordingly, this study chemically monitored for the first time a series of 17 endocrine disruptor compounds (EDCs). The target EDCs include natural (17β-oestradiol, oestrone) and pharmace...
Article
Full-text available
This study describes the simultaneous quantification of 56 pesticides in surface coastal water, supported by the development and validation of a gas chromatography (GC)–ion trap (IT) mass spectrometry (MS) method. Samples (500 mL) were pre-concentrated 2500 times by solid phase extraction (OASISTMHLB). The compounds were identified and quantified,...
Article
Full-text available
Two novel methods were developed to extract and quantify 56 pesticides in surface waters, considering their content in both dissolved aqueous phase (DAP) and suspended particulate matter (SPM) fractions. These procedures were applied to coastal samples taken seasonally during 2012-2013, from three strategic sampling sites along the Ria Formosa Lago...
Article
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The Tagus is the longest Iberian river, notwithstanding, the levels of natural and xenoestrogenic endocrine-disrupting compounds (EDCs) were never measured in its estuary. Suspecting for their presence, we made a major survey of 17 EDCs that include: (i) natural (17β-oestradiol and estrone) and pharmaceutical oestrogens (17α-ethynylestradiol); (ii)...
Article
Full-text available
The Lima River is a Spanish-Portuguese water body. Notwithstanding the fact that the river incorporates protected natural areas, levels of endocrine-disrupting compounds (EDCs) within its waters have never been measured; such EDCs include the following: natural and pharmaceutical oestrogens (17β-estradiol, E1, and 17α-ethynylestradiol), industrial...
Article
A new and fully validated QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) extraction and gas chromatography-tandem mass spectrometry methodology was developed and subsequently implemented for the quantification of 16 polycyclic aromatic hydrocarbons (PAHs) in wild (from Matosinhos Beach, Portugal) and commercial (from Ria de Arousa, Spai...
Article
Full-text available
The Mondego River estuary demonstrates signs of pollution, but the levels of endocrine disrupting compounds (EDCs), such as the natural (17β-estradiol and estrone) and pharmaceutical (17α-ethynylestradiol) estrogens, xenoestrogenic industrial pollutants (4-octylphenol, 4-nonylphenol, and their mono- and diethoxylates and bisphenol A), phytoestrogen...
Article
Full-text available
A newly analytical method based on QuEChERS extraction followed by gas chromatography with mass spectrometry (GC-MS) analysis was developed and validated for the quantification of 18 PCBs in wild (from Matosinhos Beach, Portugal) and cultivated (from Ria de Arousa, Spain) mussel samples, pooled by sex. Wild animals showed higher PCB levels than cul...
Article
Full-text available
A gas chromatography with mass spectrometric detection (GC-MS) method was developed and optimized for the determination of 17 endocrine disrupting compounds (EDCs) in coastal water samples. The evaluated EDCs were from different origins and included estrogens, bisphenol A, alkylphenolethoxylates, alkylphenols, phytoestrogens and sitosterol (SITO)....
Article
Full-text available
In spite of its outstanding ecological and touristic importance the Ria Formosa Lagoon shows signs of anthropogenic pollution. Nonetheless, until the present survey no studies had ever documented the measurement of natural and pharmaceutical estrogens (17β-estradiol, estrone, and 17α-ethynylestradiol), xenoestrogenic industrial pollutants (4-octylp...
Article
Full-text available
The Ave River flows through the most urbanized and industrialized Porto district areas. Despite ovotestis was recently reported in male fish from the estuary, no data exist on the water levels of natural and pharmaceutical estrogens (17β-estradiol, estrone, and 17α-ethynylestradiol), xenoestrogenic industrial pollutants (4-octylphenol, 4-nonylpheno...
Article
Full-text available
The Sado waters show signs of anthropogenic pollution. However, hardly any information exists on the levels of endocrine disrupters (EDCs) such as estrogens (17β-estradiol, estrone and 17α-ethynylestradiol) and there is no information at all on xenoestrogenic industrial pollutants (4-octylphenol, 4-nonylphenol and their mono and diethoxylates and b...
Article
Despite its wide use in toxicology, a detailed characterization of RTL-W1 cell line lagged behind leaving ambiguities about its cell origin. We aimed to better characterize the line regarding cell phenotype and tumorigenic state. We studied RTL-W1 cells in monolayers and in (4-22-week-old) aggregates considering: (a) morphology (light and electron...
Article
Scrobicularia plana da Costa, 1778, a commercially important bivalve species in southern Europe, is commonly found along the NE Atlantic and Mediterranean coasts. Like other intertidal mollusk species, it has a wide distributional range and high potential for larval dispersal. However, S. plana has a patchier distribution than most co-distributed s...
Article
In natural environments fish populations are exposed to many potential xenoestrogens, whereby understanding the impacts of mixtures continue to be of great interest. The main objective of this study was, therefore, to understand whether and how an environmentally relevant mixture of xenoestrogens found in the Douro River estuary can disrupt the nor...
Article
Qualitative and quantitative approaches were tested to assess zebrafish liver effects after sub-acute exposures of certain pharmaceuticals. Carbamazepine, fenofibric acid, propranolol, sulfamethoxazole and trimethoprim were tested individually and in mixtures, including low environmental levels. Overall, data showed sex specific reactions in liver,...
Article
In natural environments fish are exposed to endocrine disrupting compounds (EDCs) present at low concentrations and with different modes of actions. Here, adult zebrafish of both sexes were exposed for 21 days to an estrogenic mixture (Mix) of eleven EDCs previously quantified in Douro River estuary (Portugal) and to 100 ng/L 17α-ethinylestradiol (...
Article
Full-text available
The Leça River and two beaches close to the north and south margins of the estuary, nowadays an important seaport harbor, show signs of contamination. However, the chemical nature of that contamination is uncertain. Therefore, this study checked for the presence of 11 endocrine disrupting compounds (EDCs) of animal (17β-estradiol and estrone), phar...
Article
Full-text available
A simple low-cost, analytical method based on microwave-assisted extraction of sediments, followed by solid phase micro-extraction and gas chromatography mass spectrometry, was developed and validated for the quantification of sixteen polycyclic aromatic hydrocarbons (PAHs) in marine and estuarine sediment samples. The PAHs were those included in t...
Article
Fish embryos are a particularly vulnerable stage of development, so they represent optimal targets for screening toxicological effects of waterborne xenobiotics. Herein, the toxicity potential of two mixtures of pharmaceuticals was evaluated using a zebrafish embryo test. One of the mixtures corresponds to an environmentally realistic scenario and...
Article
Concerns associated with pharmaceuticals in aquatic systems demand the establishment of links between xenobiotics and their respective concentrations and impacts on aquatic organisms. Herein, effects of non-steroidal pharmaceuticals in the gonadal maturation of zebrafish (Danio rerio) were evaluated by histopathological and stereological analyses a...
Poster
”We invite you to join us at the SETAC Europe 21st Annual Meeting in the Milano Convention Centre in Milan, Italy from 15-19 May 2011: Ecosystem Protection in a Sustainable World: a Challenge for Science and Regulation” http://milano.setac.eu/?contentid=291
Article
Full-text available
Previous studies in the Douro River estuary, based on occasional sampling, showed the presence of several estrogenic disrupting chemicals (EDCs). In sequence, we hypothesized that such type of pollution was more likely an enduring issue than an occasional phenomenon, and that it may even affect recreational beaches in each side of the estuarine mou...
Article
Full-text available
An analytical method based on solid-phase extraction followed by gas chromatography mass spectrometry (GC-MS) was developed and validated for the quantification of 39 pesticides with distinct physico-chemical characteristics (including some degradates), in estuarine water samples. The method includes pre-concentration of samples (2500-fold factor),...
Article
Full-text available
Fisheries, PO Box 338, 6700 AH Wageningen, The Netherlands 2) University of Trás-os-Montes e Alto Douro, Zootechnic Engineering, 5000 Vila Real, Portugal 3) Burgers'Zoo, Antoon van Hooffplein 1, 6816 SH Arnhem, The Netherlands 4) INVE Technologies NV, Hoogveld 93, 9200 Dendermonde, Belgium 5) Ghent University, Dept of Animal Production, Proefhoeves...

Questions

Questions (2)
Question
I am not sure how can I relate my data (quantified amounts of pesticides in bivalves) with the acceptable daily intake (ADI-mg/kg bw/day) established by the European Union and if I should consider the maximum residue levels (MRL).
Per example:
Pesticide alachlor: 0.0053 mg/kg
ADI: 0.005 mg/kg bw/day
Human weight: 60 kg
MRL: 0.01 mg/kg
Could someone enlighten me?
Question
I would like to predict the environmental risk from an annual monitoring study in pesticides. Accordingly to Backhaus and Faust (2012) I should calculate the CA and independent action (IA) to compare with my results, but I cannot find a clear example about it. Could someone help me?

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