
Castro JohnboscoUniversity of Twente | UT · Department of Developmental Bioengineering
Castro Johnbosco
Master of Science
About
18
Publications
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Introduction
Castro Johnbosco currently works at the Department of Developmental Bioengineering at University of Twente.
Additional affiliations
Education
September 2016 - October 2019
August 2011 - May 2015
Publications
Publications (18)
Mechanotransduction is widely used to guide cell fate in hydrogels. Traditionally, hydrogels contain adhesive ligands that dynamically bond with cells to stimulate biochemical signaling axes such as YAP-TAZ. However, the molecular toolbox to achieve mechanotransduction remains virtually limited to non-covalent bonds, which limits the ability to pro...
Extracellular matrix (ECM) guides cell behavior and tissue fate. Cell populations are notoriously heterogeneous leading to large variations in cell behavior at the single‐cell level. Although insights into population heterogeneity are valuable for fundamental biology, regenerative medicine, and drug testing, current ECM analysis techniques only pro...
Mechanotransduction is widely used to guide cell fate in hydrogels. Traditionally, hydrogels contain adhesive ligands that dynamically bond with cells to stimulate biochemical signalling axis such as YAP-TAZ. However, the molecular toolbox to achieve mechanotransduction has remained virtually limited to non-covalent bonds, which limits our ability...
The vascular tree is crucial for the survival and function of large living tissues. Despite breakthroughs in 3D bioprinting to endow engineered tissues with large blood vessels, there is currently no approach to engineer high‐density capillary networks into living tissues in a scalable manner. Here, photoannealing of living microtissue (PALM) is pr...
Osteoarthritis (OA) is a degenerative disease of the joints for which no curative treatment exists. Intra-articular injection of stem cells is explored as a regenerative approach, but rapid clearance of cells from the injection site limits the therapeutic outcome. Microencapsulation of mesenchymal stem cells (MSCs) can extend the retention time of...
Incorporating non-invasive biosensing features in organ-on-chip models is of paramount importance for a wider implementation of these advanced in vitro microfluidic platforms. Optical biosensors, based on Bioluminescence Imaging (BLI), enable continuous, non-invasive, and in-situ imaging of cells, tissues or miniaturized organs without the drawback...
Oxygenating biomaterials can alleviate anoxic stress, stimulate vascularization, and improve engraftment of cellularized implants. However, the effects of oxygen-generating materials on tissue formation have remained largely unknown. Here, we investigate the impact of calcium peroxide (CPO)-based oxygen-generating microparticles (OMPs) on the osteo...
Engineered living microtissues such as cellular spheroids and organoids have enormous potential for the study and regeneration of tissues and organs. Microtissues are typically engineered via self‐assembly of adherent cells into cellular spheroids, which are characterized by little to no cell–material interactions. Consequently, 3D microtissue mode...
Biopolymer-based thermoresponsive injectable hydrogels with multifunctional tunable characteristics containing anti-oxidative, biocompatibility, anti-infection, tissue regeneration, and/or anti-bacterial are of abundant interest to proficiently stimulate diabetic wound regeneration and are considered as a potential candidate for diversified biomedi...
Hydrogel coatings can improve the biocompatibility of medical devices. However, stable surface bonding and homogeneity of hydrogel coatings are often challenging. This study exploits the benefits of biohybrid hydrogels of crosslinked four-armed poly(ethylene glycol) and heparin to enhance the hemocompatibility of cobalt-chromium (CoCr) vascular ste...
Double-network (DN) hydrogels are fabricated from poly(2-ethyl-2-oxazoline) (PEOXA)-peptide conjugates, which can be enzymatically crosslinked in the presence of Sortase A (SA), and physical networks of alginate (Alg), yielding matrices with improved mechanical properties with respect to the corresponding PEOXA and Alg single networks, and excellen...
Biomedical implants, especially the hip and knee implants, have been evolved over the years with great significance in terms of its biocompatibility and corrosion resistance. These implants, however, fail at their longevity when they encounter with the tissue, which is generally due to the failure in coatings over the surface of the implants. Moreo...
The roles of biodegradable materials have been increasing due to its promising and improved features than conventional materials used for biomedical implants. Thus, the need for developing a better biodegradable material is necessary which could deliver better properties for the implants. This present study aims to develop a better biodegradable ma...
Questions
Questions (3)
Hello Everyone
I would like to do a reaction between a amine compound (Michael donor) and vinyl sulfonated polysacchride (Michael acceptor). So is it possible to do a click reaction with use of a base catalyst if so what kind of catalyst should I use?, and if anyone can recommend a reaction protocol .
Hello everyone.
I would like to extract RNA from MSC's retrived from hydrogel. The cell number would be very low as less than 100 cells, any suggestions on kits available for RNA extraction for such low cell numbers or alternate protocols except the trizol method would be very helpful.
(i corr) value and Tafel constants Calculations