Caroline Duchaine

Caroline Duchaine
Laval University | ULAVAL · Department of Biochemistry and Microbiology

About

350
Publications
38,002
Reads
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6,292
Citations
Citations since 2017
110 Research Items
3626 Citations
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20172018201920202021202220230200400600800

Publications

Publications (350)
Article
Full-text available
Objective While facing personal protective equipment (PPE) shortages during the COVID-19 pandemic, several institutions looked to PPE decontamination and reuse options. This study documents the effect of two hydrogen peroxide treatments on filtration efficiency and fit tests as well as the side effects for volunteers after the decontamination of N9...
Article
Antibiotic resistance in bacteria is becoming a major sanitary concern worldwide. The extensive use of large quantities of antibiotics to sustain human activity has led to the rapid acquisition and maintenance of antibiotic resistant genes (ARGs) in bacteria and to their spread into the environment. Eventually, these can be disseminated over long d...
Preprint
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We isolated infectious SARS-CoV-2 from aerosol samples collected from hospital rooms of COVID19 patients. Isolated virus successfully replicated in cell cultures 14 months after collection, opening up prospects for retrospective analyses of samples stored during the previous waves of COVID-19.
Article
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Wastewater treatment plants (WWTPs) are confirmed sources of bioaerosols and can be a hotspot for both antibiotic-resistant bacteria and antibiotic-resistant genes (ARGs). Bioaerosols can be a source of dispersion for bacteria and ARGs into the environment. Biofiltration is one of the most effective technologies to mitigate odors from WWTPs. The ob...
Article
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Antimicrobial resistance (AMR) is continuing to grow across the world. Though often thought of as a mostly public health issue, AMR is also a major agricultural and environmental problem. As such, many researchers refer to it as the preeminent One Health issue. Aerial transport of antimicrobial-resistant bacteria via bioaerosols is still poorly und...
Article
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Monitoring antibiotic resistance genes (ARGs) is vital to the One Health approach to tackling the antibiotic resistance crisis. It has been suggested that conifer needles can be used as passive bioaerosol samplers. Here, the use of conifer needles as biomonitors of ARGs in bioaerosols was assessed as a proof-of-concept. Needles were collected from...
Article
During wastewater treatment, bioaerosols are generated and, can either remain in suspension for several hours or settle on surfaces and workers may be exposed. The presence of pathogens in the air could contribute to an increased frequency of gastrointestinal or respiratory illness amongst workers. Due to harsh winter conditions in Eastern Canada,...
Article
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Background The different clinical manifestations, from none to severe, and the variability in efficacy of SARS-CoV-2 diagnosis by upper respiratory tract testing, make diagnosis of COVID-19 and prevention of transmission especially challenging. In addition, the ways by which the virus can most efficiently transmit still remain unclear. Case Presen...
Article
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Wastewater treatment is one of the major biotechnological processes used to treat municipal and industrial sewage. All the steps involved in the removal of contaminants from wastewaters to treat municipal and industrial sewage represent a reservoir of a dynamic microbial communities with specific key players in the different process types. Aerosoli...
Article
Between 2007 and 2012, hospitalization rate related to respiratory system diseases in children ≤1–year‐old was near 7 times higher in Nunavik compared with the whole province of Quebec. To assess the impact of poor indoor air quality (IAQ) in residential environments on children's respiratory health, the Nunavik's intervention study investigated th...
Article
Background We determined the burden of SARS-CoV-2 in air and on surfaces in rooms of patients hospitalized with COVID-19 and investigated patient characteristics associated with SARS-CoV-2 environmental contamination. Methods Nasopharyngeal swabs, surface, and air samples were collected from the rooms of 78 inpatients with COVID-19 at six acute car...
Article
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Public bus system worldwide transports daily more than 34 million people; therefore, air quality inside buses is an important urban air pollution issue. Airborne microbiological composition and abundance was examined inside five different fuel type buses (diesel (EURO IV and EURO V), hybrid diesel (HD), compressed natural gas (CNG), hybrid natural...
Article
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The use of axenic animal models in experimental research has exponentially grown in the past few years and the most reliable way for confirming their axenic status remains unclear. It is especially the case when using individual ventilated positive-pressure cages such as the Isocage. This type of cage are at a greater risk of contamination and expo...
Article
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Influenza and RSV are human viruses responsible for outbreaks in hospitals, long-term care facilities and nursing homes. The present study assessed an air treatment using ozone at two relative humidity conditions (RHs) in order to reduce the infectivity of airborne influenza. Bovine pulmonary surfactant (BPS) and synthetic tracheal mucus (STM) were...
Article
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Lung dendritic cells (DCs) are divided into two major populations, which include CD103+XCR1+ cDC1s and CD11b+Sirpα+ cDC2s. The maintenance of their relative proportions is dynamic and lung inflammation, such as caused by exposure to lipopolysaccharide (LPS), a component of the outer membrane of Gram-negative bacteria, can have a significant impact...
Article
Each year, healthcare settings are confronted by outbreaks caused by Influenza and infected patients produce aerosols making this route a potential issue during outbreaks. This study was conducted in an Eastern Canadian hospital during three confirmed influenza outbreaks. Air was sampled in the rooms of infected patients using a liquid cyclone samp...
Preprint
Full-text available
Background The aim of this prospective cohort study was to determine the burden of SARS-CoV-2 in air and on surfaces in rooms of patients hospitalized with COVID-19, and to identify patient characteristics associated with SARS-CoV-2 environmental contamination. Methods Nasopharyngeal swabs, surface, and air samples were collected from the rooms of...
Article
Indoor air quality is a major issue for public health, particularly in northern communities. In this extreme environment, adequate ventilation is crucial to provide a healthier indoor environment, especially in airtight dwellings. The main objective of the study is to assess the impact of ventilation systems and their optimization on microbial comm...
Article
Following recent findings linking the human gut microbiota to gastrointestinal cancer and its treatment, the plausible relationship between lung microbiota and pulmonary cancer is explored. This study aims at characterizing the intratumoral and adjacent healthy tissue microbiota by applying a 16S rRNA gene amplicon sequencing protocol to tissue sam...
Article
A gap exists between good laboratory practises with axenic animals and procedures applied. This work aimed at choosing the appropriate disinfectant between sodium dichloroisocyanurate (MB-10) and potassium peroxymonosulfate (Virkon<sup>™</sup>) disinfectants and to adjust the soaking time of the material used with ISOcage biosafety stations. Anothe...
Article
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The SARS-CoV-2 pandemic has created a troublesome issue for employees in biochemistry clinical laboratories due to fears of aerosol generation during sample treatment. This study was designed to assess aerosol production during the pre-analytical procedures for blood and urine samples using a model bacterium. Air sampling and surface swabbing were...
Article
Volume 34, no. 1, e00184-20, 2021, https://doi.org/10.1128/CMR.00184-20. Page 9, last sentence of second paragraph of the “Epidemiological Studies on Transmission” subsection: “basic PCI measures beyond PPE” should read “basic IPC measures beyond PPE.” Page 26: Reference 99 should appear as shown below. 99. Park BJ, Peck AJ, Kuehnert MJ, Newbern C,...
Article
A better description of airborne transmission routes of viruses that are responsible for nosocomial infections requires efficient and accurate sampling methods that allow the preservation of viral integrity and infectivity. The aim of this project was to compare the virus collection efficiency of traditional filter sampling using cassettes with wat...
Article
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The lack of methodological standardization diminishes the validity of results obtained and the conclusions drawn when studying the lung microbiota. We report the validation of a complete 16S rRNA gene amplicon sequencing workflow, from patient recruitment to bioinformatics, tailored to the constrains of the pulmonary environment. We minimize the im...
Article
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Background Long-term care facilities (LTCF) are environments particularly favourable to coronavirus disease (SARS-CoV-2) pandemic outbreaks, due to the at-risk population they welcome and the close proximity of residents. Yet, the transmission dynamics of the disease in these establishments remain unclear. Methods Air and no-touch surfaces of 31 r...
Article
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The worldwide repercussions of COVID-19 sparked important research efforts, yet the detailed contribution of aerosols in the transmission of SARS-CoV-2 has not been elucidated. In an attempt to quantify viral aerosols in the environment of infected patients, we collected 100 air samples in acute care hospital rooms hosting 22 patients over the cour...
Article
Since the beginning of the COVID-19 pandemic, there has been intense debate over SARS-CoV-2’s mode of transmission and appropriate personal protective equipment for health care workers in low-risk settings. The objective of this review is to identify and appraise the available evidence (clinical trials and laboratory studies on masks and respirator...
Article
Air filtration to remove viruses is considered for use in the swine industry to reduce epidemic episodes in Canada. The capture efficiencies of commercially available air filters against biological particles (bioaerosols) such as viruses needs to be determined in standardized and controlled conditions such as the ones required by the ASHRAE Standar...
Article
Recycled manure solids (RMS) produced in dairy farms from fresh manure need to be sanitized before using them as bedding material. However, the impact on air quality of composting RMS remains unknown. Four RMS composting methods were tested during a 10-day aging of piles in experimental chambers: static windrow (SW), turned windrow (TW), SW followi...
Article
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High-throughput DNA sequencing (HTS) has changed our understanding of the microbial composition present in a wide range of environments. Applying HTS methods to air samples from different environments allows the identification and quantification (relative abundance) of the microorganisms present and gives a better understanding of human exposure to...
Article
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Background: Most of electronic cigarette (e-cigarette) users are also smoking tobacco cigarettes. Due to the relative novelty of this habit, very little is known on the impact of vaping on pulmonary health, even less on the potential interactions of dual e-cigarette and tobacco cigarette use. Methods: Therefore, we used well-established mouse mo...
Article
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Reported indoor air quality (IAQ) complaints are common even in relatively new or renovated school buildings in Finland. However, detecting the causes for complaints with commonly used indoor air measurements is difficult. This study presents data on perceived and measured IAQ in six comprehensive school buildings in Finland. The aim of this study...
Article
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There is currently an ongoing worldwide pandemic of a novel virus belonging to the family of Coronaviruses (CoVs) which are large, enveloped, plus-stranded RNA viruses. Coronaviruses belong to the order of Nidovirales, family of Coronavirinae and are divided into four genera: alphacoronavirus, betacoronavirus, gammacoronavirus and deltacoronavirus....
Article
Introduction: Workers' exposure to metalworking fluid (MWF) has been associated with respiratory disease. As part of a public health investigation of a manufacturing facility, we performed paired environmental and human sampling to evaluate cross-pollination of microbes between environment and host and possible effects on lung pathology present am...
Article
Ozone is effective against bacteria and viruses although its influence over bioaerosols is understudied and could be useful particularly in agricultural buildings such as swine confinement buildings. Ozone treatment of air within ventilation plenum could be applied for biosecurity purposes during the quarantine of incoming animals. In this study, a...
Article
Background: In a proportion of patients with hypersensitivity pneumonitis, biological and environmental factors sustaining inflammation are ill-defined, resulting in no effective treatment option. Bioaerosols found in occupational settings are complex and often include Toll-like receptor ligands, such as endotoxins. How Toll-like receptor ligands...
Article
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This study was designed to test the efficacy of an air treatment using ozone and relative humidity (RH) for the inactivation of airborne viruses. Four phages (φX174, PR772, MS2 and φ6) and one eukaryotic virus (murine norovirus MNV-1) were exposed to low ozone concentrations (1.23 ppm for phages and 0.23 ppm for MNV-1) and various levels of RH for...
Article
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This paper presents the performance of two eukaryotic genomic ribosomal regions, ITS1 and ITS2, in describing fungal diversity in aerosol samples using amplicon-based High-Throughput Sequencing (HTS). Composting sites, biomethanization facilities, and dairy farms, all affected by the presence of fungi, were visited to collect air samples. The ampli...
Article
Highlights Bench-scale pig buildings are suitable for studying airborne contaminants of commercial pig buildings. Multi-replicate designed experiments are possible using bench-scale pig buildings (experimental chambers). Strategies to reduce airborne contaminants may be developed in a timely and cost-effective way using the chambers. Abstract. Pig...
Technical Report
Le traitement des eaux usées comporte plusieurs étapes susceptibles de générer des bioaérosols auxquels des travailleurs peuvent être exposés. Bien que la littérature à ce sujet soit relativement abondante, les connaissances relatives au risque, à l’exposition et à la fréquence de certaines maladies chez cette population demeurent mal documentées....
Article
Because bioaerosols are related to adverse health effects in exposed humans and indoor environments represent a unique framework of exposure, concerns about indoor bioaerosols have risen over recent years. One of the major issues in indoor bioaerosol research is the lack of standardization in the methodology, from air sampling strategies and sample...
Article
Full-text available
Homes with inadequate ventilation and indoor air quality (IAQ) are particularly common in northern and remote communities. Previous studies have observed that the indoor air in these homes can have elevated concentrations of CO 2 , environmental tobacco smoke, and elevated relative humidity leading to mold issues. These conditions may cause various...
Article
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Experimental aerosol generation methods aim to represent natural processes; however the complexity is not always captured and unforeseen variability may be introduced into the data. The current practices for natural and experimental aerosol generation techniques are here reviewed. Recommendations for best practice are presented, and include charact...
Article
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The release of pathogens into the air from swine confinement buildings are mitigated through preventative measures, such as outgoing air filtration, to reduce the risk of spread to nearby barns and communities. The present study aims to characterize the effectiveness of a percolating biofilter developed by the Research and Development Institute for...
Article
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Objectives: Methanogenic archaea have been found to make up part of the bioaerosols in pig, cattle, and poultry farms. So far no attempts have been made to determine how season, farm type, and farm characteristics may affect workers' exposure to archaea. Methods: Personal filter samples from 327 farmers working on 89 Danish farms were analysed f...
Article
Bioaerosols collected in a liquid-based sampler can be re-aerosolized into the airflow during collection and lost or concentrated in the collection fluid and overestimated during the subsequent analyses. Very little information is available concerning the specific impact on bioaerosol quantification and diversity and wrong evaluation of pathogens o...
Article
Full-text available
Pig farming practices in Eastern Canada changed drastically into intensified and specialized operations. In large confined finishing facilities, workers are now exposed to concentrated biological particles (bioaerosols) for prolonged periods of time. Occupational exposure to airborne dust, endotoxins, bacteria, human pathogenic agents (Staphylococc...
Article
Full-text available
Electronic cigarette uses propylene glycol and glycerol to deliver nicotine and flavors to the lungs. Given the hundreds of different brands, the thousands of flavors available and the variations in nicotine concentrations, it is likely that electronic cigarette settings and e-liquid composition affect the size distribution of particles emitted and...
Article
Full-text available
Background: Bioaerosols are a major concern for public health and sampling for exposure assessment purposes is challenging. The nasopharyngeal region could be a potent carrier of long-term bioaerosol exposure agents. This study aimed to evaluate the correlation between nasopharyngeal bacterial flora of swine workers and the swine barns bioaerosol b...
Article
This work shed light on a significant issue regarding the loss of fungal spores when recovered from air samples using liquid medium and centrifugation to concentrate air particles before DNA extraction. We provide proof that the loss affects the overall fungal diversity of aerosols and that some taxa are differentially more affected than others. Fu...
Article
Bioaerosols are recognized as one of the main transmission routes for infectious diseases and are responsible for other various types of health effects through inhalation and potential ingestion. Associating exposure to bioaerosol and health problems is challenging and adequate exposure monitoring is a top priority for aerosol scientists. The multi...
Article
Full-text available
The transmission of infectious microbes via bioaerosols is of significant concern for both human and animal health. However, gaps in our understanding of respiratory pathogen transmission and methodological heterogeneity persist. New developments have enabled progress in this domain, and one of the major turning points has been the recognition that...
Article
Full-text available
Background: The importance of aerosols in the spread of viruses like influenza is still a subject of debate. Indeed, most viruses can also be transmitted through direct contact and droplets. Therefore, the importance of the airborne route in a clinical context is difficult to determine. The aim of this study was to design a chamber system to study...
Article
Recent technological advances in the dairy industry have enabled Canadian farms with liquid manure systems to use mechanical solid-liquid separation paired with composting of the separated solids for on-farm production of low-cost bedding material. However, as several approaches are available, it is difficult for farmers to select the appropriate o...
Presentation
While bioaerosols are a major concern for public health, accurately assessing human exposure is challenging. Highly contaminated environments, such as pig buildings, contain a broad diversity of aerosolized bacteria that may impact human health. The nasopharyngeal region may represent a long-term reservoir of inhaled bioaerosols. The herein study a...
Conference Paper
Full-text available
Exposure to bioaerosols is connected to the development of respiratory diseases, and fungi play an important part in it. Fungal aerosols were first studied by culture methods. These classical methods induce a bias in fungal diversity: they do not represent all risks faced by exposed persons when they are in the presence of non-viable and/or non-cul...
Article
Occupational exposure to harmful bioaerosols in industrial environments is a real threat to the workers. In particular, dairy-farmworkers are exposed to high levels of fungal bioaerosols on a daily basis. Associating bioaerosol exposure and health problems is challenging and adequate exposure monitoring is a top priority for aerosol scientists. Usi...
Article
Bioaerosol studies aim to describe the microbial content and increase understanding of the aerosolization processes linked to diseases. Air samplers are used to collect, identify, and quantify bioaerosols. Studies comparing the performances of air samplers have typically used a culture approach or have targeted a specific microorganism in laborator...
Presentation
Full-text available
Real-time measurement of microbial aerosols in workplaces using fluorescent aerodynamic particle-size
Poster
While bioaerosols are a major concern for public health, accurately assessing human exposure is challenging. Highly contaminated environments, such as pig buildings, contain a broad diversity of aerosolized bacteria that may impact human health. The nasopharyngeal region may represent a long-term reservoir of inhaled bioaerosols. The herein study a...
Technical Report
La biométhanisation est un procédé de transformation anaérobie des matières organiques putrescibles (MOP) en biogaz et en digestat destiné à l'épandage en milieu agricole. Ce procédé est en forte croissance en raison de la Politique québécoise de gestion des matières résiduelles qui prévoit le bannissement de l'enfouissement des MOP, et ce, dès 202...
Technical Report
L’exposition aux bioaérosols de différents milieux de travail n’est pas étrangère au développement de maladies respiratoires; les moisissures y tiendraient d’ailleurs un rôle important. De par les activités qui y sont menées, certains milieux de travail génèrent de grandes quantités d’aérosols (incluant des moisissures), qui proviennent de la matiè...