Carolina Lucci

Carolina Lucci
University of Brasília | UnB · Department of Physiological Sciences

PhD

About

117
Publications
20,131
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2,538
Citations

Publications

Publications (117)
Article
The molecular mechanisms regulating follicular development and ensuring primordial follicle activation remain undefined. To help elucidate these mechanisms, this proteomic study of bovine ovarian tissue identified the differential molecular profiles of preantral follicles together with the spatial distribution of the most abundant molecular compone...
Article
Full-text available
Controlling populations of free-roaming dogs and cats poses a huge challenge worldwide. Non-surgical neutering strategies for male animals have been long pursued, but the implementation of the procedures developed has remained limited to date. As submitting the testes to high temperatures impairs spermatogenesis, the present study investigated loca...
Article
This study aimed to evaluate the effects of an intratesticular injection of silver nanoparticles (AgNPs) on reproductive parameters and health of rats, and to evaluate the AgNPs biodistribution in order to develop a nanotechnological contraceptive agent for male animals. Treated animals received 220 μL of AgNPs solution (0.46 µg-Ag/ml) in each test...
Article
Full-text available
The present study evaluated revascularization time of fresh and cryopreserved cat ovarian tissue after transplantation to subcutaneous tissue. Ovaries of five cats were used and eight pieces of ovarian tissue were taken from each pair of ovaries. Immediately after removal, three pieces were transplanted and one fixed for fresh control. The remainin...
Article
Climatic variables can trigger physiological, biochemical, haematological and hormonal alterations that influence the maintenance of homeothermy and can affect production and productivity in sheep. Different mechanisms are responsible for tolerance to heat stress (HS) including coat and skin colour, body size, fat distribution, physiological reacti...
Article
The aim of this study was to analyse the reproductive aspects of male bats of three common species of the Phyllostomidae family: Artibeus lituratus, Platyrrhinus lineatus and Sturnira lilium, during dry and rainy months in a specific area of the Cerrado biome. Body weight was significantly higher during the dry months for S. lilium. The gonadosomat...
Article
Full-text available
The aim of this study was to assess a slow-freezing protocol of cat ovarian tissue cryopreservation using autotransplantation. Four adult queens were ovariohysterectomized and the ovaries were fragmented and cryopreserved. After one week, the grafts were thawed and autografted to the subcutaneous tissue of the dorsal neck of each queen, then random...
Data
Cats show phylogenetic and physiological similarities to felids that are under risk of extinction; in addition, their reproductive tissue is widely available after spaying is performed in veterinary clinics. We used ovaries of domestic cats with the aim of developing a tailored method for cryopreserving this type of tissue. By developing this techn...
Poster
Full-text available
Most species in the Felidae family are at risk of extinction, with the exception being the domestic cat, which has become an excellent model for reproductive studies due to its phylogenetic proximity to other felids. Several assisted reproduction techniques have been developed to aid in the conservation of these species. However, the lack of detail...
Article
Cutaneous hemangiosarcoma is a malignant neoplasia that frequently occurs in dogs. The most effective treatment requires wide surgical excision of the tumor. To avoid mutilating surgeries, photodynamic therapy (PDT) could serve as an alternative treatment. This study aimed to treat cutaneous hemangiosarcomas in dogs using PDT with aluminium-chlorid...
Article
Full-text available
Chemical oocyte enucleation holds the potential to ease somatic cell nuclear transfer (SCNT), although high enucleation rates remain limited to micromanipulation-based approaches. Therefore, this study aimed to test mitomycin C (MMC) for use in bovine functional chemical oocyte enucleation. Incubation of denuded eggs in 10 µg ml ⁻¹ MMC for differen...
Article
Background: Cryopreservation of human ovarian tissue has been increasingly applied worldwide to safeguard fertility in cancer patients, notably in young girls and women who cannot delay the onset of their treatment. Moreover, it has been proposed to patients with benign pathologies with a risk of premature ovarian insufficiency. So far, more than...
Article
Somatic cell nuclear transfer (SCNT) allows animal cloning but remains technically challenging. This study investigated limitations to functional oocyte enucleation by actinomycin D (AD) as a means of making SCNT easier to perform. Denuding oocytes or inhibiting transcription before AD treatment revealed that the toxicity of this compound during bo...
Article
Research question: Could a modification in the ovarian tissue freezing protocol improve follicle survival after cryopreservation and xenotransplantation? Design: Ovarian tissue was used from 13 adult patients, frozen either with our original protocol, or a modified version involving a higher concentration of dimethyl sulphoxide (DMSO), larger vo...
Article
Full-text available
Cryobiology is the study of life at subzero temperatures. But our interest on it is other than creating new lifestyles for survival in glacial environments. Proteins, cells, small pieces of tissues or whole organs can be stored at very low temperatures and that allow taking advantage, in the future, of sources we have available today. An example of...
Article
Ovarian tissue cryopreservation is a promising technique for fertility maintenance. The aim of this study was to compare the morphology of domestic cat ovarian follicles after tissue cryopreservation with ethylene glycol (EG) and dimethyl sulfoxide (Me2SO). Ovaries from healthy adult cats undergoing elective ovariohysterectomy were used. Eight frag...
Article
Itraconazole(ITZ) – an antifungal agent of the azole class – is clinically used to treat a variety of fungal infections. Among them, paracoccidioidomycosis (PCM), a fungal infection caused by Paracoccidioides brasiliensis, is the most prevalent systemic fungal infection in Brazil as well as in many countries in South America. Conventional treatment...
Article
The aim of this study was to define the population, morphological and ultrastructural characteristics of bitch preantral follicles (PAFs) and to compare the effects on the morphology of PAF of two cryopreservation techniques - slow freezing (SF) and vitrification (V) - of bitches' ovarian tissue. The average population (number per ovary) of PAFs wa...
Article
Ovarian tissue transplantation could be a valuable technique for the preservation of endangered animals. The domestic cat affords an adequate experimental model for studies aimed at wild felids due to its phylogenetic similarity. Thus, this pilot study evaluated the efficacy of cat ovarian tissue autotransplantation to a peripheral site. Three adul...
Article
Full-text available
Visualization and clear understanding of the ovarian structures are important in determining the stage of oestrus, helping to diagnose several pathologies and supporting advances in reproductive technologies. In this research, computerized microtomography (microCT) was used to explore and characterize the ovarian structure of seven mammalian specie...
Article
Magnetic nanoparticles can be used in different areas of biology. It is therefore important to know the effects of such nanomaterials on germline cells as they may traverse the blood-testis barrier. This work aimed to evaluate the response of bull sperm after exposure to a magnetic fluid containing DMSA-coated maghemite nanoparticles (MNP-DMSA) in...
Article
Study question: Are mouse preantral follicles differently affected by isolation, encapsulation and/or grafting procedures according to stage? Summary answer: Isolated secondary follicles showed superior ability to survive and grow after transplantation, which was not related to a particular effect of the isolation and/or grafting procedure, but...
Article
Full-text available
Cottonseed coproducts contain gossypol which can have specific effects upon the male reproductive system. We evaluated the reproductive development of male lambs close to puberty fed on three cotton coproducts. Twenty-four 5-month old male lambs received four diets: 20 % of dry matter intake of whole cottonseed (WCS), high oil cottonseed meal (CSC)...
Conference Paper
This study aimed to evaluate the efficacy of fresh and frozen-thawed cat ovarian tissue autotransplantation to a peripheral site (CEUA protocol 76940/2012). Seven queens were subjected to ovariohysterectomy. Ovaries of three queens were fragmented and immediately autografted to the subcutaneous tissue of the dorsal neck region (Fresh group). For th...
Poster
Full-text available
Since there is no specific immunohistochemistry markers for domestic cat ovarian tissue, this poster indicates the brands and concentrations that were efficient for reproductive studies in this species.
Article
In vitro culture (IVC) and transplantation procedures are essential protocols employed in the evaluation of ovarian follicle survival and development. Culture in the chorioallantoic membrane (CAM) of chick embryos is an intermediate method that provides important follicle development information and has not been tested for cat ovaries to date. The...
Article
Full-text available
In this study, a multivariate analysis of morphological and physiological characteristics was performed on clinically healthy rams from six breeds (Santa Ines, Bergamasca, Dorper, Texel, Ile de France and Hampshire Down) to determine if these characteristics were able to separate and determine the most important variables in the differentiation of...
Article
Heat is a major factor limiting the production of animals in the tropics. Sheep are well adapted to diverse ecosystems, however, temperature and relative humidity can influence animal husbandry. Therefore, this study was carried out to verify the thermoregulation in rams of six breeds (Bergamasca, Dorper, Ile de France, Hampshire Down, Santa Ines a...
Article
Full-text available
Natural products have been sources of numerous drugs over the history. However, little is known about the therapeutic potential of Amazon forest species. This work aimed at testing the potential of the hydrophobic extract of Arrabidaea chica (ACE), an Amazon plant, as a source of photosensitizers for anticancer photodynamic therapy. ACE was tested...
Article
Full-text available
The ultrastructural analysis of oocytes and ovarian follicles has been used to evaluate the effects of assisted reproductive techniques, such as cryopreservation or in vitro oocyte maturation. It also benefits the understanding of such complex mechanisms that occur during folliculogenesis. From the beginning of primordial follicles growth until ooc...
Article
Morphometric characterization of nanoparticles is crucial to determine their biological effects and to obtain a formulation pattern. Determining the best technique requires knowledge of the particles being analyzed, the intended application of the particles, and the limitations of the techniques being considered. The aim of this article was to pres...
Article
Full-text available
Iron oxides that exhibit magnetic properties have been widely studied not only from an academic standpoint, but also for numerous applications in different fields of knowledge, such as biomedical and technological research. In this work, magnetite and maghemite nanoparticles were synthesized by chemical coprecipitation of FeCl 2 ·4H 2 O and FeCl 3...
Article
Full-text available
The once bred ewe slaughter method proposes the use of female lamb to produce a lamb and then both are slaughtered, increasing income and high quality meat production. Thus, this study evaluated the growth and reproduction performance of ewe lamb from Santa Inês (SI), a naturalized genetic resource, and their crosses (Dorper x Santa Inês (DOR), Tex...
Article
Full-text available
The aim of the present study was to describe the morphology of the cervix in Santa Ines ewes. One hundred cervices were collected from females of different ages (12 to 48 months). Five types of cervical os were observed: duck-bill, flap, rosette, papilla and spiral, with duck-bill being the most predominant type (51%) overall. The average cervix le...
Article
This study evaluates the post-hatching development of in vitro-produced (IVP) embryos until Day 14. On Day 7, IVP embryos were either transferred to recipient uteruses or placed in a post-hatching development (PHD) system. As a control group, in vivo-produced (IVV), Day-7 embryos were also transferred to recipient uteruses. All groups were collecte...
Article
Full-text available
Immobilization, used in clinical practice to treat traumatologic problems, causes changes in muscle, but it is not known whether changes also occur in nerves. We investigated the effects of immobilization on excitability and compound action potential (CAP) and the ultrastructure of the rat sciatic nerve. Fourteen days after immobilization of the ri...
Article
Studying the interactions among growth factors that act in the ovary is a fundamental step to clarify the mechanisms controlling follicle development. Thus, the aim of this study was to evaluate the effect of the interaction between keratinocyte growth factor-1 (KGF-1) and kit ligand (KL) on early folliculogenesis in the goat. Goat ovarian fragment...
Article
Even though sheep embryo cryopreservation is a commonly used procedure the survival and pregnancy outcomes can vary greatly. This study investigated whether cryopreservation was causing subtle changes in ultrastructure, mitochondrial activity or cytoskeletal integrity. Sheep embryos were either slow cooled in 1.5M EG (n = 22), or vitrified in 20% E...
Article
This work aimed to develop solid lipid nanoparticles (SLNs) loaded with doxorubicin evaluating the influence of docosahexaenoic acid (DHA), a polyunsaturated fatty acid that enhances the activity of anticancer drugs, on drug encapsulation efficiency (EE). The SLN were characterized for size, zeta potential, entrapment efficiency (EE) and drug relea...
Article
Full-text available
Nanosized maghemite particles were synthesized, precoated (with dimercaptosuccinic acid) and surface-functionalized with anticarcinoembryonic antigen (anti-CEA) and successfully used to target cell lines expressing the CEA, characteristic of colorectal cancer (CRC) cells. The as-developed nanosized material device, consisting of surface decorated m...
Article
Full-text available
The present study was designed to compare Day 14 bovine embryos that were produced entirely in vitro using the post-hatching development (PHD) system with in vivo-derived embryos without or with transient PHD culture from Day 7 to Day 14. Embryos on Day 14 were used for sex determination and gene expression analysis of PLAC8, KRT8, CD9, SLC2A1, SLC...
Article
The aim of this study was to describe the ultrastructure of sheep oocytes during 24 h of in vitro maturation (IVM). Ovaries were collected from a slaughterhouse and the follicles aspirated. The selected cumulus oocyte complexes (COC's) were divided into groups and transferred to maturation medium, where they were maintained for 6, 12, 18 or 24 h. C...
Article
Full-text available
Alterations to DNA methylation have been attributed to in vitro culture and may affect normal embryo development. We chose to analyze DNA methylation reprogramming in the rabbit which, of the species with delayed transcriptional activation of the embryonic genome, allows easy comparisons between in vivo-developed (IVD) and in vitro-cultured (IVC) e...
Article
Breast tumors represent the most common malignant tumors. Current treatments for humans and pets rely on tumor excision and adjuvant chemotherapy, which may affect both cancer cells and normal cells. Photodynamic therapy (PDT) is an approved treatment modality for a variety of cancers and was recently recommended as a first-line treatment for non-m...
Article
Full-text available
Goat ovarian cortex fragments were subjected to slow freezing in the presence of various solutions containing intracellular cryoprotectants, including 1.0 M ethylene glycol (EG), propanediol (PROH), or dimethyl sulfoxide (DMSO), with or without sucrose and/or fetal calf serum (FCS). Histological examination revealed that only the DMSO-containing so...
Article
The objective of this study was to characterize the morphometry and ultrastructure of porcine preantral and antral follicles, especially the lipid component evolution. Ovarian tissue was processed for light microscopy. Ovarian tissue and dissected antral follicles (< 2, 2-4, and 4-6 mm) were also processed for transmission electron microscopy using...
Article
The reprogramming of DNA methylation in early embryos has been considered to be essential for the reprogramming of differentiated parental genomes to totipotency, the transcription of embryonic genome activation (EGA) and subsequent development. However, its degree appears to differ as a function of species and it may be altered by the in vitro env...
Article
Full-text available
The objective of this study was to compare morphological characteristics, kinetics of development, and gene expression of male and female IVP embryos that were cultured until day (D)15 (fertilization = D0), using either phosphate-buffered saline (PBS) or Milli-Q water (MQW) to dilute the agarose gel used for tunnel construction. On D11, embryos (n...
Article
Full-text available
This study describes the morphological, morphometric and ultrastructural characteristics of the Amazonian manatee Trichechus inunguis (Natterer, 1883) spermatozoon. The spermatozoa were obtained from a urine sample of an adult T. inunguis kept in captivity. The spermatozoa were analyzed by light and transmission electron microscopy. The head of Ama...
Article
Full-text available
Four groups of Santa Inês male sheep born in four seasons in the Distrito Federal were analyzed to evaluate the age and body weight at puberty, at which point were castrated. Histological measurements were carried out on the testicles to evaluate cellular parameters and size measurements of the seminiferous tubules. The effect of the group on cellu...
Article
Full-text available
Foram analisados quatro grupos de ovinos machos da raça Santa Inês nascidos em quatro estações do ano, no Distrito Federal, a fim de observar as idades e o peso corporal em que estes atingiram a puberdade. A partir dos cortes histológicos dos testículos, foram analisados parâmetros celulares e mensurações dos túbulos seminíferos. O efeito da data d...