Carole Calas-Blanchard

University of Perpignan | UPVD · IMAgES Institute of Modelling and Analysis in Geo- Environment and Health

The conditions of wastewater treatment by photo-oxidation are addressed in this work with the aim of highlighting the importance of the matrix, concentration and cocktail effects of pharmaceutical pollutants very present in wastewater treatment plant. The idea is to operate under real conditions, i.e. effluent from the WWTP, representative concentr...

Senior residences are health-care facilities that are socially-accepted for the assistance of elderly people. Since the elderly account for the foremost pharmaceutical-consuming age-group, senior residences become a hot-spot for pharmaceuticals discharge to the sewage grid. The objectives of the present study were to identify the bioactive pharmace...

The bis‐triazole ligand and its corresponding copper complexes were synthesized and characterized for the first time and proposed as new labels for the development of electrochemical aptasensors. The bis‐triazole ligand was prepared from methyl 1,6‐heptadiyne‐4‐carboxylate and 2‐(azidomethyl)phenol using classical CuAAC in presence of different cop...

BACKGROUND The occurrence of pharmaceutical compounds in wastewater has become a major concern for human health and the environment. Therefore, it is an important challenge to improve conventional wastewater treatment to remove these compounds. Coupling biological treatment with advanced oxidation technology has been widely studied in the literatur...

In this research, different olive paste treatments, including physical treatments such as ultrasounds or microwaves as well as chemical treatments with addition of sodium citrate, were proposed. Therefore, the main objective of this work was to compare different treatments and optimize various classical and original spectrophotometric methods in or...

This study aims to contribute to the risk management of pharmaceuticals in the environment, illustrating risk perceptions of lay people and experts from Southwestern Europe (Portugal, Spain, and France). The psychometric paradigm was applied to assess risk regarding four hazards: pharmaceuticals in the environment (i.e., broadly framed), pharmaceut...

Scanning electrochemical microscopy (SECM) is discussed as a versatile tool to provide a unique approach of localized electrochemical information in the context of biosensing research. The step‐by‐step immobilization of DNA aptamer with intrinsic redox activity on screen‐printed carbon electrode (SPCE) was successfully monitored using SECM imaging...

Metallic complexes of macrocycles chelators 1,4,7-triazacyclononane-N,N,N-triacetic acid (NOTA) and 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) were synthetized with iron (III) giving Fe(III)-DOTA and Fe(III)-NOTA complexes. They were studied in comparison of ferricyanide and ferrocenemethanol on cyclic voltammetry with glassy c...

The presence of pharmaceuticals in the environment is an emergent unknown environmental problem, linked to increased production and consumption of pharmaceuticals and, as such, understanding risk perception is fundamental. This study focuses on exploring causes (trust and knowledge) and effects (intention and proper individual disposal of pharmaceu...

The aim of this work was to evaluate the impact of the microwave treatment as well as the effect of sodium citrate as co-adjuvant on legal parameters. The addition of microwaves (72 seconds, followed by 20 minutes of malaxation) results a low oxidation of olive oil and, consequently, a reduction in the peroxide value compared with the conventional...

This work presents the synthesis and characterization of salen/salan metal complexes for their future application as electrochemical labels in affinity sensors. Due to its stability and electrochemical properties, oxovanadium salan...

A label-free electrochemical aptasensor was fabricated for reagentless determination of tetracycline (TET) in water. A 5′-amino-modified 76-mer oligonucleotide was immobilized on screen-printed carbon electrode via carbodimide coupling. Cyclic voltammetry experiments revealed an unexpected electroactivity of immobilized aptamer, showing a quasi-rev...

Electrochemical molecularly imprinted polymers (e-MIPs) were for the first time introduced in screen-printed carbon electrodes (SPCE) as the sensing element for the detection of an organic pollutant. To play this sensing role, a redox tracer was incorporated inside the binding cavities of a cross-linked MIP, as a functional monomer during the synth...

One of the main pursuits, yet most difficult, in monitoring studies is to identify the sources of environmental pollution. In this study, we have identified health-care facilities from south European countries as an important source of pharmaceuticals in the environment. We have estimated that compounds consumed in by the elderly and released from...

New chemiluminescence-based immunoassays for sensitive detection of 17-β estradiol (E2) and ethinylestradiol (EE2) are described on the basis of the use of biotinylated estrogen derivatives. Estrogen derivatives bearing a carboxylic group (E2-COOH and EE2-COOH) on C-3 position were synthesized, covalently bound to aminated biotin and subsequently i...

Molecularly imprinted polymers (MIPs) for phosmet insecticide were synthesized by batch polymerization. The affinity of functional monomers to phosmet was tested using an original method involving an electrochemical biosensor based on acetylcholinesterase inhibition. It was demonstrated that association of phosmet with appropriate functional monome...

An amperometric biosensor was designed for the determination of sulcotrione, a β-triketone herbicide, based on inhibition of hydroxyphenylpyruvate dioxygenase (HPPD), an enzyme allowing the oxidation of hydroxyphenylpyruvate (HPP) in homogentisic acid (HGA). HPPD was produced by cloning the hppd gene from Arabidopsis thaliana in E. coli, followed b...

This review summarises the main electrochemical sensors and biosensors developed for assessing the reactive oxygen (ROS) and nitrogen (RNS) species of interest in clinical and physiological analysis. Among these species, a special attention is focused on sensors for the detection of the highly reactive superoxide radicals O2.−, the very stable hydr...

Oxovanadium complexes are presented as new labels for the development of electrochemical immunosensors. The concept was successfully applied to the accurate detection of estradiol, an emerging environmental pollutant, at concentrations ranging from 4 ng L(-1) to 5 μg L(-1).

A novel biosensor based on the immobilization of commercially available tyrosinase onto carbon black paste electrode was developed. This device showed significantly reduced noise compared to enzymatic sensors based on traditional graphite paste electrodes. Using catechol as the substrate, the response was linear in the range 0.013 to 150 µmol L−1 w...

Reactive oxygen species (ROS) are very unstable molecules generated during the metabolism. They can be produced in excess, contributing to cellular dysfunctions. Antioxidants are substances that play an important role as a health-protecting factor, limiting the lesions caused by ROS. Two kinds of electrochemical biosensors based on ROS have been de...

Poly(3,4-ethylenedioxythiophene) (PEDOT) and its derivatives are relatively new, and unique members of conducting-polymers family. In this article, we present an approach for simple, reliable and cost-efficient electrochemical biosensor for real-time detection and quantification of phenolic compounds (PhCs). The PEDOT:poly(styrene sulfonate) (PSS)...

A tyrosinase-based biosensor was constructed by immobilizing the enzyme on diazonium-functionalized screen-printed gold electrodes. Under optimized conditions, the biosensor exhibited rapid response to the changes in the concentration of all the tested phenolic compounds (catechol, catechin, caffeic acid and gallic acid). Sensitivity, linear range...

This paper describes the development of a xanthine oxidase/poly-m-phenylenediamine (XOD-PPD)-modified electrode. The biosensor was constructed by encapsulating XOD in a sol-gel matrix deposited onto a platinum based screen-printed electrode functionalized with a permselective PPD membrane. The hydrogen peroxide generated as a final product of the e...

An amperometric biosensor based on laccase, from Trametes versicolor (LTV), was developed and optimized for monitoring the phenolic compounds content in tea infusions. The fungal enzyme was immobilized by entrapment within polyvinyl alcohol photopolymer PVA-AWP (azide-unit pendant water-soluble photopolymer) onto disposable graphite screen-printed...

Free radicals are highly reactive molecules generated during cellular metabolism. However, their overproduction results in oxidative stress, a deleterious process that can damage cell structures, including lipids and membranes, proteins and DNA. Antioxidants respond to this problem, scavenging free radicals. This chapter critically reviews the elec...

The food industries need rapid and affordable methods to assure the quality ofproducts and process control. Biosensors, combining a biological recognition element and a sensitive transducer, are versatile analytical tools that offer advantages as classical analytical methods due to their inherent specificity, selectivity and simplicity. This paper...

This paper describes the characterization and optimization of an amperometric cytochrome c (cyt c)-based sensor for the determination of the antioxidant capacity of pure substances and natural samples. The cyt c and the xanthine oxidase (XOD) enzyme were co-immobilized on the electrode using the combination of several long-chain thiols. The self-as...

A simple and sensitive electrochemical method for the determination of antioxidant capacity has been studied. 4-hydroxybenzoic acid (4-HBA) was used as a trapping agent for photogenerated .OH radicals, leading to 3,4-dihydroxybenzoic acid (3,4-DHBA). Square-wave voltammetry was used to quantify the amount of 3,4-DHBA formed from the reaction betwee...

This paper describes the development of an amperometric cytochrome c (cyt c)-based biosensor and its later application to the quantification of the scavenging capacity of antioxidants. The enzymatic biosensor was constructed by covalently co-immobilizing both cyt c and XOD on a mercaptoundecanol/mercaptoundecanoic acid (MU/MUA) mixed self-assembled...

Oxidative stress arises from the excess of free radicals due to environmental or behavioural stressors, or simply to a malfunction of the antioxidant production. It produces damage to lipids, proteins or DNA, impeding normal cell functioning and leading to numerous human diseases, as well as to the aging process. Antioxidants respond to this proble...

The present work deals with the use of the porous texture of expanded natural graphite (ENG) as transducer in order to design electrochemical biosensors. The sensing element is a NAD+-dependent dehydrogenase. An electrochemical pretreatment of the ENG is favorable because it allows on one hand generating functional surface groups that may act as me...

Three strategies have been compared to produce screen-printed amperometric detectors for NADH: mixing Meldola Blue (MB) in the screen-printing ink, incorporation of MB-Reinecke salt (MBRS) in the graphite ink and electrodeposition of films of MB-derived polymer (poly (MB)) on electrode surface. Following modification of graphite electrodes the medi...

The present work describes a novel approach for the textural characterisation of a carbonaceous porous medium. Due to the electronic conduction properties of expanded natural graphite (ENG), electrochemical determination was proposed and the use of cyclic voltammetry enables determination of the surface area and porous volume of ENG. First, experim...

A monoenzymatic amperometric biosensor was developed for the detection of acetaldehyde. The sensor is based on the association of screen-printed carbon electrodes and aldehyde dehydrogenase immobilized by a sol-gel entrapment method. Modification of screen-printed carbon electrodes with Reinecke salt of Meldola's Blue (MBRS) resulted in highly sens...