Carol Kyle

James Hutton Institute · SEGS

In this paper, we look at people's engagement with peatlands and peatland restoration in Scotland through the lens of care. Care is an elementary aspect of how we relate to each other and the world around us. Our results highlight the importance of personal experiences and relationships and embodied learning in fostering and defining care. The resu...

Agriculture provides many public goods; however the costs and benefits of these are rarely well distributed. Maintaining public good provision often relies on external governance mechanisms, in turn reliant on the existing socio-ecological system. With two groups of stakeholders (practitioners and academics) we created cognitive maps of socio-ecolo...

Water resources in Scotland and across the EU are under increasing stress, especially in areas with intense agriculture. Global climate changes are likely to exacerbate water shortages and cause an increasingly unpredictable supply. Hence, there is an increasing interest in wastewater reclamation. Wastewater reclamation has the potential to conserv...

It is known that feeding frequency affects the insulin status of lactating ruminants (Sutton et al , 1988), and that this may influence milk composition. It is unclear, however, whether these effects are due to modulation of mammary metabolism, or regulation of substrate distribution. The present study aimed to establish whether the partition of bl...

The mammary gland of lactating ruminants (Guinard & Rulquin 1994) does not appear to extract sufficient quantities of free amino acids (AA) to account for their output as milk protein. Based upon application of a precursor (blood or plasma free AA):product (casein) labelling technique (Backwell et al . 1996) in goats, results suggest that blood pep...

Based on Habermas' Theory of Communicative Action, this paper critiques the transparency and legitimacy of participatory scenario planning, considering a case study of scenario development for the livestock industry within Scotland. The paper considers the extent to which the case study approximates the conditions for ‘ideal speech situations’ and...

The development of fetal ovarian follicles is a critical determinant of adult female reproductive competence. Prolonged exposure to environmental chemicals (ECs) can perturb this process with detrimental consequences for offspring. Here we report on the exposure of pregnant ewes to an environmental mixture of ECs derived from pastures fertilized wi...

In this paper, we present a description of foresighting activities undertaken by EPIC, Scotland's Centre of Expertise on Animal Disease Outbreaks, to investigate the future uncertainty of animal health security in the Scottish sheep and cattle sectors. Using scenario planning methodologies, we explored four plausible but provocative long-term futur...

Private water supplies provide ~3% of the Scottish population with their main source of drinking water. Many more people encounter private supplies when they stay in holiday accommodation and visit the more remote parts of Scotland, although PWS can be found in urban areas. The quality of these supplies is variable; many have adequate treatment and...

On the 11 th June in Victoria Quay, Edinburgh, the 5th EAWG (Ecosystem Approach Working Group) was held. In total 25 researchers and policy makers attended (see Appendix 1 for attendee list and emails). The meeting aimed to provide a forum for policy makers and strategic planners to learn about the research conducted at the James Hutton Institute...

Biosolids (processed human sewage sludge), which contain low individual concentrations of an array of contaminants including heavy metals and organic pollutants such as polyaromatic hydrocarbons (PAH), polychlorinated biphenols (PCB) and polychlorinated dibenzodioxins (PCDD)/polychlorinated dibenzofurans (PCDF) known to cause physiological disturba...

A neural network approach was used to predict the presence and concentration of a range of endocrine disrupting compounds (EDCs), based on field observations. Soil sample concentrations of endocrine disrupting compounds (EDCs) and site environmental characteristics, drawn from the National Soil Inventory of Scotland (NSIS) database, were used. Neur...

Exposure of female fetuses to environmental chemicals (ECs) during pregnancy results in a disturbed ovarian adult phenotype. We investigated the influence of pre- and/or post-conception exposure to low-level mixtures of ECs on the structure and function of the fetal ovine ovary. We examined ovarian morphology, expression of oocyte and granulosa cel...

Background The aim of this study was to determine the effects of maternal undernutrition on ovine fetal testis morphology and expression of relevant histological indicators. Maternal undernutrition, in sheep, has been reported, previously, to alter fetal ovary development, as indicated by delayed folliculogenesis and the altered expression of ovari...

Liver tissue concentrations of selected polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs) and polycyclic aromatic hydrocarbons (PAHs) were determined in groups of Texel ewes and lambs following exposure to pastures fertilised with either sewage sludge (Treated; T) or inorganic fertiliser (Control; C). Lambs were slaughtered a...

This work was supported by the European Community 'S Seveneth Framework Programme (FP/2007-2013) agreement n° 212885

This work was supported by the EC7th Framwork Programme (FP7/2007-2013) grant agrement n° 212885

A selective pressurized liquid extraction (SPLE) method, followed by gas chromatography-mass spectrometry (GC-MS), for the simultaneous extraction and clean-up of estrone (E1), 17β-estradiol (E2), 17α-ethynylestradiol (EE2), estriol (E3) and bisphenol A (BPA) from soil samples is described. The on-line clean-up of soil by SPLE was achieved using di...

Muscle tissue was collected from ewes and lambs derived from farms throughout Scotland and sample concentrations of five endocrine disrupting compound groups were determined. Farms of origin were categorised according to geographic region. There were few statistically-significant differences with region or distance from cities. However, the magnitu...

Liver concentrations of selected pollutant classes were determined in groups of sheep fetuses and their dams, at 55 (Experiment 1) and 110 (Experiment 2) days of gestation (term = 145 d) following exposure, throughout their breeding lives and after mating, to pasture treated with either inorganic fertiliser (control, CC) or with sewage sludge (trea...

Pregnant ewes were maintained on pastures fertilized, twice yearly, with either sewage sludge (2.25tonnes dry matter/ha; Treated; T) or inorganic fertilizer containing equivalent amounts of nitrogen (Control; C), to determine effects on maternal and fetal bone structures, density and mechanical properties of exposure to environmental concentrations...

Fetal tissue concentrations of endocrine disrupting compounds (EDCs), and their relationship to maternal tissue concentrations, are largely unknown, in any species. In particular, the patterns of accumulation in the respective tissues following increased rates of environmental exposure are little known. This study was designed to determine fetal an...

The femurs of male and female sheep (Ovis aries), aged 18 months, bred on pastures fertilized twice annually with sewage sludge (2.25 tonnes dry matter/ha; Treated; T)) or on pastures treated with inorganic fertilizer (Control; C) were studied, using peripheral Quantitative Computed Tomography (pQCT) and the three-point bending test. Males were mai...

The acute phase response is the immediate host response to infection, inflammation and trauma and can be monitored by measuring the acute phase proteins (APP) such as haptoglobin (Hp) or serum amyloid A (SAA). The plane of nutrition during pregnancy is known to affect many mechanisms including the neuroendocrine and neuroimmune systems in neonatal...

Concentrations of endocrine disrupting compounds (EDCs) of two classes, the alkyl phenols (nonyl phenol (NP) and octyl phenol (OP)) and phthalates, in the milk of ewes grazed on pastures fertilised with sewage sludge or with inorganic fertiliser were determined at three stages of lactation. Milk concentrations of these compounds varied greatly betw...

The liver is a major metabolic and endocrine organ of critical importance in the regulation of growth and metabolism. Its function is determined by a complex interaction of nutritionally regulated counter-regulatory hormones. The extent to which hepatic endocrine sensitivity can be programed in utero and whether the resultant adaptations persist in...

The effects of nutrition on patterns of live-weight change, hair follicle activity, moult, hormone profiles and associated activities of monodeiodinase enzyme types II and III (MDII and MDIII) in cashmere goats were investigated. From 1 week before the winter solstice (mid December), one group of 15 animals was given a ration designed to provide 2·...

This study aimed to determine whether reduced fetal ovary folliculogenesis in ewes undernourished during early/midpregnancy is associated with altered ovarian cell proliferation and/or the expression of apoptosis-regulating genes. Groups of ewes (n = 11-19) were fed either 100% (high; H) or 50% (low; L) of metabolisable energy requirements for live...

The purpose of this study was to evaluate whether experimental exposure of pregnant sheep to a mixture of environmental chemicals added to pasture as sewage sludge (n = 9 treated animals) exerted effects on fetal testis development or function; application of sewage sludge was undertaken so as to maximize exposure of the ewes to its contents. Contr...

Concentrations of potentially toxic metals (PTMs) in the livers of pregnant, mature ewes and their 110 day foetuses, and in 6-month old lambs born to other ewes, were determined. The animals had been reared on pastures fertilized for 5 years with either sewage sludge (treated; T) or conventional inorganic fertilizer (control; C). The effects of tre...

We studied selected tissues from ewes and their lambs that were grazing pastures fertilized with either sewage sludge (treated) or inorganic fertilizer (control) and determined concentrations of alkylphenols and phthalates in these tissues. Mean tissue concentrations of alkylphenols were relatively low (< 10-400 microg/kg) in all animals and tissue...

Nutritional feedback provided by systemic hormones, such as insulin and leptin, influences reproductive neuroendocrine output within the hypothalamus, yet the mechanisms and their interaction with photoperiodic cues remain unresolved in seasonal species. Here, peripheral glucose (G) infusion was used to increase endogenous concentrations of insulin...

Epidemiological and animal studies strongly indicate that the environment experienced in utero determines, in part, an individual's likelihood of developing cardiovascular disease in later life. This risk has been further linked to impaired kidney function, as a result of compromised development during fetal life. The present study therefore examin...

The prenatal nutritional environment influences the subsequent risk of hypertension in adulthood. Animal studies have used, generally, the rat as a model species to illustrate the association between maternal nutrient intake and blood pressure in the resulting adult offspring. No study to date has shown programming of adult cardiovascular function...

The roles of skin deiodinase enzymes in the expression of genotypic differences in hair follicle activity and cashmere fibre growth and moult were studied in 9 Siberian (S) and 10 Scottish cashmere (SC) goats. The proportion of animals exhibiting cashmere growth was significantly greater in S than in SC goats in both late July (P < 0.001; early in...

The effects of season and of manipulations of circulating prolactin concentrations on the expression of monodeiodinase type II (MDII) and monodeiodinase type III (MDIII) expression in the skin of cashmere goats were investigated. Deiodinase enzymes control the rates of synthesis and degradation of triiodothyronine (T3), a hormone implicated in the...

Body reserves (long-term) and food intake (short-term) both contribute nutritional feedback to the hypothalamus. Reproductive neuroendocrine output (GnRH/LH) is stimulated by increased food intake and not by high adiposity in sheep, but it is unknown whether appetite-regulating hypothalamic neurons show this differential response. Castrated male sh...

A model of Leu and protein metabolism by the mammary gland and hind leg of lactating goats was constructed and evaluated from data collected by using [15N, 1-13C]Leu kinetics measured during amino acid (AA) infusion and a hyperinsulinemic-euglycemic clamp (IC). Goats were given continuous intravenous infusions of either saline or AA (65 g/d) for 7....

The aim of this study was to determine the effects of maternal undernutrition during pregnancy on adult reproductive function in male and female offspring. Groups of ewes were fed rations providing either 100% (High, H) or 50% (Low, L) of estimated metabolisable energy (ME) requirements for pregnancy, from mating until day 95 of gestation, and ther...

The aim of this study was to determine the effects of maternal undernutrition, applied during physiologically relevant stages of development of the reproductive system, on reproductive development in male sheep fetuses. Groups of ewes (n = 11-19) were fed rations providing either 100% (high; H) or 50% (low; L) of metabolizable energy requirements f...

The aims of this study were to determine which hormones may have a role in the expression of maternal undernutrition effects on reproductive function, in both the developing fetus and the adult offspring. This was undertaken by measuring the effects of long-term maternal undernutrition on metabolic hormone profiles and pituitary responses to single...

An arteriovenous technique, combined with a 30-h i.v. infusion of [5-(13)CH3]Met and [5,5,5-(2)H]Leu, was used to monitor mammary uptake of free amino acid (AA) and to estimate the proportion of casein synthesized from circulating peptides in goats in early and late lactation. At both stages, kinetics was performed on the last day of consecutive 5....

Soil concentrations of dioctyl phthalate (DOP) and the alkyl phenols, octyl phenol (OP) and nonyl phenol (NP), after repeated surface applications of sewage sludge to pastures, were investigated. Liquid sludge was applied at a rate of 2.25 tonnes dry matter (DM) per hectare to each of three treated (T) plots on three occasions during the summer and...

Gonad development in female sheep fetuses is thought to occur in a number of key stages. The aim of this study was to determine the effects of maternal undernutrition, applied at one or more of these critical stages, on fetal ovarian development. Groups of ewes (n = 11-19) were fed rations providing either 100% (high; H) or 50% (low; L) of energy r...

We investigated the roles of insulin and amino acid (AA) in regulating milk production and the uptake of AA and blood flow (BF) by the mammary gland and hind-leg of goats (n = 4). During two periods, either saline or AA (65 g/d) was infused i.v. for 7.5 d, and, beginning on d 5, goats were subjected to a hyperinsulinemic-euglycemic clamp. The insul...

Groups of 15 adult, castrated, male Soay sheep were housed under natural daylength conditions at 57 degrees N and fed a complete diet ad libitum (AL) or at a restricted rate (R) of 35 g dry matter (DM)/kg(0.75) initial liveweight per day. The diet was based on barley and dried grass pellets and contained an estimated 11.6 MJ of metabolisable energy...

An arteriovenous kinetics technique was used to monitor mammary gland lysine and protein metabolism in goats (n = 4) at two stages of lactation (80 +/- 17 vs. 233 +/- 14 DIM) in response to an i.v. infusion of lysine (Lys) plus methionine (Met). At each stage of lactation [2-15N] and [1-13C; 6,6-2H2] Lys kinetics were performed on the last day of 5...

The contribution to casein biosynthesis of peptides derived from blood was examined in late lactation goats (254 to 295 d in milk). Ratios of mammary uptake of free amino acids (AA) in blood to output of AA in milk protein and ratios of the enrichments of Phe, Tyr, Met, and Lys at isotopic plateau in secreted milk casein to the free AA in arterial...

Energy expenditure was estimated using the doubly-labelled water (DLW) method in summer in five free-living adult, non-pregnant, non-lactating, red deer (Cervus elaphus) hinds (weight 107.3 (SE 0.9) kg; age 6 (SE 1) years) on lowland pasture under typical farming conditions. Climatic conditions were monitored throughout the experiment. Errors due t...

It is known that feeding frequency affects the insulin status of lactating ruminants (Sutton et al , 1988), and that this may influence milk composition. It is unclear, however, whether these effects are due to modulation of mammary metabolism, or regulation of substrate distribution. The present study aimed to establish whether the partition of bl...

The mammary gland of lactating ruminants (Guinard & Rulquin 1994) does not appear to extract sufficient quantities of free amino acids (AA) to account for their output as milk protein. Based upon application of a precursor (blood or plasma free AA):product (casein) labelling technique (Backwell et al . 1996) in goats, results suggest that blood pep...

Ovariectomized, oestradiol-implanted Soay ewe lambs from 21 September (aged 21 weeks) had restricted (liveweight maintenance) (n = 4) or unrestricted food (n = 4); ovary-intact lambs had unrestricted food (n = 8). LH activation in ovariectomized lambs on restricted and unrestricted food and onset of ovulatory cycles in ovary-intact lambs all occurr...

Castrate male sheep (wethers, average liveweight 38 ± 0·6 kg) were given one of the following diets for 10 weeks followed by euthanasia ( n =8/group): high-energy high-protein providing 1·5 times the energy required to maintain liveweight (maintenance) (group 1·5M), low-energy low-protein at 0·5 maintenance (0·5M), or low-energy high-protein at 0·5...

Red deer display characteristic seasonal changes in appetite, growth, and reproduction which are mediated by the pineal hormone, melatonin, which provides a direct neuroendocrine transduction of the ambient photoperiod. To identify potential central sites of action for this hormone, [2(-125)I]iodomelatonin binding sites were localized and character...

Red deer calves were reared from birth to 16 months in either constant intermediate 12L: 12D daylength (ID) or in natural photoperiod (NP) (four males and four females per group) to investigate effects on the somatotropic and reproductive axes, and to compare responses between the seres. Measurements, starting from 3 months (September), were made e...

The effect of nutritional growth restriction on reproductive development in red deer stags reared in constant photoperiod was investigated and the correlation between reproductive status and circulating concentrations of growth hormone (GH) and insulin-like growth factor-1 (IGF-1) examined. Stags were reared from birth in constant photoperiod (12 h...