Carmelo Laudanna

Carmelo Laudanna
IRB Barcelona Institute for Research in Biomedicine | IRB Barcelona · Group of Growth control and cancer metastasis

Bioinformatics PhD

About

72
Publications
3,989
Reads
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544
Citations
Introduction
I am currently works at the Group of Stem Cell and Cancer, IRB Barcelona Institute for Research in Biomedicine, as Bioinformatician. The main duties are the analysis of Next Generation Sequencing data and the integration of large sets of data to better understand the role of transcriptional and epigenetic mechanisms in regulating the function of adult cancer stem cells.
Additional affiliations
March 2010 - July 2013
BIOGEM SCARL
Position
  • PhD
March 2010 - April 2013
Università degli Studi del Sannio
Position
  • PhD Student
Education
March 2010 - July 2013
Università degli Studi del Sannio
Field of study
  • Bioinformatics

Publications

Publications (72)
Article
Full-text available
The gain-of-function mutation in the pleckstrin homology domain of AKT1 (AKT1E17K) occurs in lung and breast cancer. Through the use of human cellular models and of a AKT1E17K transgenic Cre-inducible murine strain (R26-AKT1E17K mice), we have demonstrated that AKT1E17K is a bona fide oncogene for lung epithelial cells. However, the role of AKT1E17...
Article
Full-text available
Canine atopic dermatitis (AD) is a very common inflammatory skin disease, but limited data are available on the genetic characterization (somatic mutations, microarrays, and genome-wide association study (GWAS)) of skin lesions in affected dogs. microRNAs are good biomarkers in inflammatory and neoplastic diseases in people. The aim of this study w...
Article
Full-text available
Background: Improvement in genetic characterization of Colon Cancer (CC) patients is required to propose new potential targets, since surgical resection coupled to chemotherapy, presents several limits such as cancer recurrence and drug resistance. Targeted therapies have more efficacy and less toxicity than standard treatments. One of the most re...
Article
Full-text available
In the publication of this article [1], there is an error in Fig. 7. The minus and plus signals are inverted which impairs understanding of the results described.
Article
Implications: Mis-segregation of chromosomes is a prominent feature of chromosome instability and intra-tumoral heterogeneity recurrent in metastatic tumors for which the molecular basis is unknown. The present study provides insight into the mechanism by which defects in rootletin, a centrosome linker component causes tetraploid segregation error...
Article
Full-text available
The objective of this study was to investigate the mutational profiles of cancers arising in different colon segments. To this aim, we have analyzed 37 colon cancer samples by use of the Ion AmpliSeq™ Comprehensive Cancer Panel. Overall, we have found 307 mutated genes, most of which already implicated in the development of colon cancer. Among thes...
Article
Full-text available
The objective of this study was to determine the feasibility to detect copy number alterations in colon cancer samples using Next Generation Sequencing data and to elucidate the association between copy number alterations in specific genes and the development of cancer in different colon segments. We report the successful detection of somatic chang...
Article
Full-text available
Hyperactivation of the phosphatydil-inositol-3' phosphate kinase (PI3K)/AKT pathway is observed in most NSCLCs, promoting proliferation, migration, invasion and resistance to therapy. AKT can be activated through several mechanisms that include loss of the negative regulator PTEN, activating mutations of the catalytic subunit of PI3K (PIK3CA) and/o...
Data
Biofunctions enriched by exclusive DEGs in BEAS-AKT1-E17K cells. (TIFF)
Data
List of differentially expressed gene probes in BEAS-shPTEN cells. (XLS)
Data
List of differentially expressed gene probes in BEAS-AKT1-E17K cells. (XLS)
Data
List of differentially expressed gene probes in BEAS-PIK3CA-E545K cells. (XLS)
Data
List of common DEGs between BEAS-PIK3CA-E545K and BEAS-AKT1-E17K cells. (XLSX)
Data
List of common DEGs between BEAS-PIK3CA-E545K and BEAS-shPTEN cells. (XLSX)
Data
List of exclusive DEGs in BEAS-shPTEN cells. (XLSX)
Data
Biofunctions enriched by exclusive DEGs in BEAS-PIK3CA-E545K cells. (TIFF)
Data
Biofunctions enriched by exclusive DEGs in BEAS-shPTEN cells. (TIFF)
Data
List of common DEGs between BEAS-shPTEN and BEAS-AKT1-E17K cells. (XLSX)
Data
List of exclusive DEGs in BEAS-AKT1-E17K cells. (XLSX)
Data
List of exclusive DEGs in BEAS-PIK3CA-E545K cells. (XLSX)
Data
List of exclusive DEGs that enrich Cell proliferation, Invasion and Migration Biofunctions of tumour cell lines in BEAS-PIK3CA-E545K cells. (XLSX)
Data
List of exclusive DEGs that enrich Cell proliferation and Migration Biofunctions of tumour cell lines in BEAS-shPTEN cells. (XLSX)
Article
Full-text available
Hyperactivation of the PI3K/AKT pathway is observed in most human cancer including lung carcinomas. Here we have investigated the role of miRNAs as downstream targets of activated PI3K/AKT signaling in Non Small Cell Lung Cancer (NSCLC). To this aim, miRNA profiling was performed in human lung epithelial cells (BEAS-2B) expressing active AKT1 (BEAS...
Article
Full-text available
The IBTK gene encodes the major protein isoform IBTKα that was recently characterized as substrate receptor of Cul3-dependent E3 ligase, regulating ubiquitination coupled to proteasomal degradation of Pdcd4, an inhibitor of translation. Due to the presence of Ankyrin-BTB-RCC1 domains that mediate several protein-protein interactions, IBTKα could ex...
Article
Background Bloom syndrome is a rare and recessive disorder characterized by loss-of-function mutations of the BLM gene, which encodes a RecQ 3′–5′ DNA helicase. Despite its putative tumor suppressor function, the contribution of BLM to human sporadic colorectal cancer (CRC) remains poorly understood. Methods The transcriptional regulation mechanism...
Article
Full-text available
Impairment of the immune response and aberrant expression of microRNAs are emerging hallmarks of tumour initiation/progression, in addition to driver gene mutations and epigenetic modifications. We performed a preliminary survey of independent adenoma and colorectal cancer (CRC) miRnoma data sets and, among the most dysregulated miRNAs, we selected...
Article
Full-text available
Colorectal cancer (CRC) is the third leading cause of cancer-related deaths worldwide, with nearly 1.4 million new cases diagnosed in 2012. CRC results from the accumulation of multiple genetic and epigenetic aberrations. Tumor localization in the large intestine tract determines different surgical approaches and treatment options. Considering the...
Article
Full-text available
Data from molecular profiles of tumors and tumor associated cells provide a model in which cancer cells can acquire the capability of avoiding immune surveillance by expressing an immune-like phenotype. Recent works reveal that expression of immune antigens (PDL1, CD47, CD73, CD14, CD68, MAC387, CD163, DAP12, and CD15) by tumor cells “immune resist...
Conference Paper
Full-text available
Colorectal cancer (CRC) is the third leading cause of cancer-related deaths worldwide, with nearly 1.4 million new cases diagnosed in 2012. CRC results from the accumulation of multiple genetic and epigenetic aberrations. Tumor localization in the large intestine tract determines different surgical approaches and treatment options. Considering the...
Conference Paper
Full-text available
The protein IBtkα is characterized by BTB-Ankyrin domains that are found prevalently in proteins of very diverse function, such as transcription regulators, ion transporters and signal transduction proteins. Recently, we demonstrated that IBTK is a component of Cul3-dependent E3 Ligase and associates with the tumor suppressor Pdcd4, a negative regu...
Article
Full-text available
INI1 (Integrase interactor 1), also known as SMARCB1, is the most studied subunit of chromatin remodelling complexes. Its role in colorectal tumorigenesis is not known. We examined SMARCB1/INI1 protein expression in 134 cases of colorectal cancer (CRC) and 60 matched normal mucosa by using tissue microarrays and western blot and categorized the res...
Article
Full-text available
MicroRNA-130b (miR-130b) is involved in several biologic processes; its role in colorectal tumorigenesis has not been addressed so far. Herein, we demonstrate that miR-130b up-regulation exhibits clinical relevance as it is linked to advanced colorectal cancers (CRCs), poor patients' prognosis, and molecular features of enhanced epithelial-mesenchy...
Article
Full-text available
We describe a novel bioinformatic and translational pathology approach, gene Signature Finder Algorithm (gSFA) to identify biomarkers associated with Colorectal Cancer (CRC) survival. Here a robust set of CRC markers is selected by an ensemble method. By using a dataset of 232 gene expression profiles, gSFA discovers 16 highly significant small gen...
Article
Peroxisome proliferator-activated receptor gamma (PPARG) inactivation has been identified as an important step in colorectal cancer (CRC) progression, although the events involved have been partially clarified. UHRF1 is emerging as a cofactor that coordinates the epigenetic silencing of tumor suppressor genes, but its role in CRC remains elusive. H...
Conference Paper
Full-text available
Signature learning from gene expression consists in selecting a subset of molecular markers which best correlate with prognosis. It can be cast as an optimization-based feature selection problem. Here we use as optimality criterion the separation between survival curves of clusters induced by the selected features. We address some important problem...
Data
NOD/SCID mice subcutaneous tumor growth. (TIF)
Data
Enriched GO terms and filtered by the Hypergeometric test and corrected using False Discovery Rate (FDR). (TIF)
Data
Cluster, containing 69 probesets, was functionally annotated by Gene Ontology (GO) for Biological Process. (TIF)
Data
Sphere assays, self renewal ability of primary lung cancer cell. (TIF)
Data
69 probesets expression profile over time. (TIF)
Data
Differential expressed probe sets of 82 genes in adherent vs spheres cell cultures. (TIF)