Carlos Alexandre Zanutto- PhD
- Researcher at State University of Maringá
Carlos Alexandre Zanutto
- PhD
- Researcher at State University of Maringá
About
59
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Introduction
Carlos Alexandre Zanutto currently works at the Departamento de Agronomia, Universidade Estadual de Maringá. Carlos does research in Molecular Biology, Virology and Microbiology. Their current project is 'Study of genetic diversity of Xanthomonas citri subsp. citri. Search for resistance sources and/or resistant varieties and study of the resistance pathways of plants to pathogen.'.
Current institution
Additional affiliations
August 2002 - present
August 2002 - October 2015
Publications
Publications (59)
O cancro cítrico, causado pela bactéria Xanthomonas citri subsp. citri é uma das doenças mais importantes da citricultura. O estudo das condições de isolamento e manutenção de isolados de X. citri subsp. citri, o conhecimento de sua exigência nutricional e escolha de um meio de cultura eficiente para seu desenvolvimento é imprescindível para poster...
O objetivo deste trabalho foi o de realizar um experimento participativo utilizando diferentes cores de armadilhas interceptadora de vôo do tipo Moericke, a fim de determinar quais são mais eficazes na relação entre pragas e inimigos naturais. O experimento foi conduzido na horta comunitária da Vila Esperança, em Maringá, com a colaboração de produ...
The present study aims to analyze, based on bibliographic sources, the possibility of using bumblebees (genus Bombus) in the pollination of tomato plants (Solanum lycopersicum L.) in vertical farms, as well as their advantages and costs. The work includes the definition of vertical farms and their structures, the description of tomato pollination m...
A Clorose variegada dos citros (CVC), é umas das principais doenças que incidem em pomares comerciais, seu agente causal é a bactéria Xylella fasdiosa, disseminada por cigarrinhas. O trabalho teve como objetivo identificar e monitorar a dispersão de cigarrinhas vetores da Xylella fastidiosa, em áreas adjacentes de pomares comerciais em parceria com...
O site 'Sistema de Treinamento em Acuidade Visual' (SiTAV), foi projetado para melhorar a precisão e confiabilidade das estimativas dos avaliadores de severidades de doenças de plantas. Descrevemos os recursos tecnológicos e procedimentos utilizados para o desenvolvimento do sistema e validação demonstrando as melhorias na estimativa de severidade...
Para determinar a eficiência de extração de compostos com capacidade antioxidante em polpas de abacate, pitaia e uva, diferentes sistemas solventes e métodos de extração foram aplicados. Realizou-se a quantificação da atividade antioxidante e compostos fenólicos totais dos extratos. A atividade antioxidante foi determinada pelo método DPPH (2,2-dif...
The present study analyzed the data compiled after the training of the precision and visual accuracy of the evaluators of both genders in plant diseases, using the SiTAV (Visual Acuity Training System) website. The training data were analyzed through the exploratory analysis, with the aid of the statistical program SAS 9.4. With 52,495 training dat...
O Huanglongbing (HLB) destaca-se como a doença mais significativa que afeta os cultivos de citros, sendo atribuível a três espécies distintas de α-proteobactérias do gênero Candidatus Liberibacter: Ca. Liberibacter asiaticus (CLas), Ca. Liberibacter americanus (CLam) e Ca. Liberibacter africanus (CLaf). No Brasil, o principal vetor para a transmiss...
A citricultura é uma das principais atividades do agronegócio brasileiro. No entanto, a produção de citros é constantemente ameaçada por doenças que causam grandes prejuízos econômicos. Uma das doenças é o cancro cítrico, causado pela bactéria Xanthomonas citri subsp. citri (Xcc), que afeta todas as variedades cítricas comerciais, não havendo métod...
Mais de uma centena de bactérias agentes de doenças de plantas estão reunidas no gênero Xanthomonas. Dentre as doenças que afetam culturas de expressão econômica, destaca-se o cancro cítrico, causado pela bactéria Xanthomonas citri subsp. citri, e a estria bacteriana do milho, causada pela Xanthomonas vasicola pv. vasculorum. As bactérias fitopatog...
O milho é reconhecidamente uma das mais importantes culturas agrícolas. Uma das recentes preocupações para à cultura é a doença foliar, de fácil disseminação, causada pela bactéria Xanthomonas vasicola pv. vasculorum (Xvv), a estria bacteriana do milho. O objetivo do trabalho foi avaliar a taxa de germinação e analisar o potencial de transmissão de...
Brazilian citrus culture became the world's largest producer of fruit since 2012. However, in all harvests it is noted that there are reports of production losses due to the attack of pathogens, among them, we highlight the citrus canker caused by the Xanthomonas citri subsp. citri bacterium. Among the various control methodologies, studying plant...
Citrus tristeza virus (CTV) can be transmitted by several aphid species in a semi-persistent mode, with Toxoptera citricida being the most efficient vector. In Brazil, mild CTV isolates are used for pre-immunization of citrus trees against severe isolates. We aimed to determine the capacity and efficiency of T. citricida in separating the viral com...
Citrus canker, caused by the bacterium Xanthomonas citri subsp. citri , is responsible for significant economic losses of citrus in Brazil and elsewhere. The disease occurs in all regions of commercial production of sweet orange ( C. sinensis L. Osbeck). Compared to chemical control, deploying canker-resistant cultivars is a viable and economical a...
Charcoal rot is an economically important fungal disease of economically important crops. Several species of the Macrophomina genus, such as M. pseudophaseolina and M. euphorbiicola, may act as
the causal agents of charcoal rot. The current study evaluates: a) the diversity of vegetative compatibility reactions in Brazilian isolates of M. pseudopha...
Aspergillus nidulans is a fungal model organism extensively used in genetic approaches. It may reproduce sexually and asexually, with a well‐defined parasexual cycle. The current paper demonstrates that the limitation of nitrogen source facilitates the production of A. nidulans’s nonmeiotic recombinants directly from heterokaryons, without the reco...
Brazil is one of the world’s largest producers of citrus. However, production is challenged due to biotic and abiotic factors limiting crop health. The aim of this work was to evaluate the influence of water deficit in sweet orange (Citrus sinensis) cv. Pera Bianchi inoculated with citrus tristeza virus (CTV). Two isolates of CTV were used, one cau...
Heterokaryosis is the association of genetically distinct nuclei in a common hyphal cytoplasm, and is a process involved in the generation of fungal variation. The fusion of the distinct nuclei within a heterokaryotic hypha produces a heterozygous diploid nucleus and is one of the stages of the parasexual cycle. A heterokaryon's viability depends o...
Guariroba is a palm species native to central Brazil. Seedlings of guariroba with leaf spots of unknown aetiology were found in Patos de Minas, Minas Gerais, Brazil. The leaf spots were manifest as two different symptom types: the first lesion type consisted of necrotic spots with a rounded to elongate shape, with a light colour and dark edges; the...
Xanthomonas citri subsp. citri, which causes Asiatic citrus canker (ACC), is an important pathogen of citrus in Brazil and elsewhere. The genetic diversity of X. citri subsp. citri pathotype 'A' has not been studied in Brazil at a local scale (up to 300 km). Forty isolates were sampled from lesions of ACC on citrus in three orchards in Paraná state...
Asiatic citrus canker (ACC, caused by the bacterium Xanthomonas citri subsp. citri Xcc) is a destructive disease of citrus in Brazil and in several other citrus-producing countries. ACC management is problematic, and bactericides such as copper can be reasonably efficacious, but do not completely control the disease. Furthermore, injury by citrus l...
Citrus genotypes show a broad range of resistance to infection with Xanthomonas citri subsp citri (Xcc), the causal agent of Asiatic citrus canker (ACC). The aim of this study was to evaluate a detached leaf inoculation method to compare nine different citrus genotypes that exhibit varied resistance levels to ACC, and to evaluate infection, coloniz...
Abstract
Asiatic citrus canker (ACC, caused by the bacterium Xanthomonas citri subsp. citri) is a destructive disease of citrus in Brazil and in several other citrus-producing countries. ACC management is problematic, and bactericides such as copper can be reasonably efficacious but do not completely control the disease. Furthermore, injury by citr...
RESUMO: O objetivo do trabalho foi avaliar genótipos cítricos com diferentes níveis de resistência ao cancro cítrico por inoculação de folhas destacadas. Um total de nove acessos de diferentes espécies cítricas foram investigados por inoculação com agulhas em folhas destacadas. Após a coleta e desinfecção das folhas, foram inoculadas por perfuração...
RESUMO: Responsável por ocasionar perdas de até 30% da produtividade, o raquitismo-da-soqueira causado pela bactéria Leifsonia xyli subsp. xyli, é uma das doenças mais importantes para a cultura da cana-de-açúcar. O objetivo deste trabalho foi comparar dois métodos de extração de DNA da bactéria Leifsonia xyli subsp. xyli afim de formular uma respo...
RESUMO: Uma das mais importantes doenças que ocorrem em citros é o cancro cítrico, afetando quase todas as variedades comerciais. A doença, causada pela bactéria Xanthomomas citri subsp. citri, provoca extensos danos à cultura e, a severidade da infecção varia de acordo com as diferentes espécies bacterianas e com as condições climáticas prevalente...
RESUMO: A análise exploratória realizada com o auxílio do programa estatístico SAS 9.4, aplicada sobre os dados de treinamento da precisão e acurácia dos avaliadores na mensuração das lesões de doenças de plantas, durante a utilização do website Sistemas de Treinamentos em Acuidade Visual (SiTAV), identificou um total de 52.495 dados de treinamento...
Huanglongbing (HLB) is considered one of the most destructive diseases of citrus as the plants rapidly become unproductive, enter a decline and eventually die. HLB is caused by the phloem-limited bacterium ‘Candidatus Liberibacter’ spp. The objective of this study was to evaluate seasonal variation of the in planta population of ‘Ca. Liberibacter a...
A limpeza clonal seguida de pré-imunização com complexos protetivos do Citrus tristeza virus (CTV) possibilitaram o cultivo comercial da laranja Pêra, variedade de relevante importância para a citricultura brasileira, mas sensível ao vírus. O emprego de isolados fracos protetivos em outras variedades de citros, mesmo com maior tolerância ao CTV, po...
Huanglongbing (HLB) is one of the most devastating citrus diseases worldwide, affecting all species of Citrus. Early characteristics of infected trees are individual branches with yellowish leaves, followed eventually by tree decline and death. The objective of this study was to examine seasonal dynamics of within-tree accumulation of 'Candidatus L...
The use of resistant genotypes is the best way to control citrus canker, caused by the plant pathogenic bacterium Xanthomonas citri susbp. citri. Genotypes can be screened for resistance to the pathogen and germplasm found to have adequate resistance can either be incorporated into breeding programs or used directly for commercial plantings. Indeed...
Resumo-O objetivo deste trabalho foi avaliar a eficiência das técnicas de " enzyme linked immunosorbent assay " (ELISA) e imunocaptura seguida da reação da polimerase em cadeia (IC-RT-PCR) na determinação do tempo necessário para que a infecção sistêmica pelo Citrus tristeza virus (CTV) possa ser detectada nas condições naturais de campo. Plantas a...
Anais Eletrônico VIII EPCC –Encontro Internacional de Produção Científica UNICESUMAR – Centro Universitário Cesumar Editora CESUMAR Maringá – Paraná – Brasil ABSTRACT: Citrus tristeza virus (CTV) is the causal agent of devastating epidemics that changed the course of the citrus industry. CTV are composed of two capsid proteins and a single-stranded...
The use of tolerant rootstocks and preimmunization has satisfactorily controlled losses associated with the Citrus tristeza virus (CTV). Several researchers have shown that CTV mild isolates that are selected in the same region where they are used are superior to isolates obtained from other areas. Thus, budwoods of 20 outstanding citrus trees were...
Citrus Tristeza Virus (CTV), causal agent of Citrus tristeza, is a Closterovirus that exist as a complex of isolates that differ in their biological and immunological properties and induces various symptoms. Multiple inoculations of the virus by the aphid vector and the moving of the infected material with severe isolates among citrus regions favor...
Plants of Pera sweet orange on Rangpur lime rootstocks, from orchards of the northwest and north of Parana state, Brazil, were evaluated for severity of symptoms and genetic diversity of Citrus tristeza virus. The severity of symptoms was evaluated by the development of tree, fruit size and stem pitting symptoms. Isolates that infect these plants w...
Aiming to evaluate the performance of different clones of Pêra sweet orange (Citrus sinensis Osbeck cv. Pêra) constituting Paraná's orchards, in relation to different pathogens, leading to the installation of an experimental orchard in northern region. This study evaluated clones performance of Pêra Vacinada and Pêra Bianchi sweet oranges, in relat...
Citrus variegated chlorosis (CVC) is the disease caused by the bacteria Xylella fastidiosa Wells. Dissemination occurs through insect vectors belonging to the order Hemiptera, family Cicadellidae (subfamily Cicadellinae), which transmit the bacteria after feeding on infected plants. The objective of this study was to identify the species of insect...
The objective of this work was to monitor the maintenance of Citrus tristeza virus (CTV) protective isolates stability in selected clones of 'Pera' sweet orange (Citrus sinensis), preimmunized or naturally infected by the virus, after successive clonal propagations. The work was carried out in field conditions in the north of Parana State, Brazil....
The aim of the present study was to assess the population flutuation of the sharpshooters species subfamily Cicadellinae belonging to the tribes Cicadellini and Proconiini, in sweet orange [Citrus sinensis( L). Osbeck] commercial orchards of the northwest region of Paraná State , Brazil. Samplings were carried out the employing every time 24 yellow...
The aim of the present study was to assess the population flutuation of the sharpshooters species subfamily Cicadellinae belonging to the tribes Cicadellini and Proconiini, in sweet orange [Citrus sinensis(L). Osbeck] commercial orchards of the northwest region of Paraná State, Brazil. Samplings were carried out the employing every time 24 yellow s...
O experimento teve como objetivo caracterizar biológica e molecularmente os isolados de CTV, obtidos de árvores de laranja Pêra em pomares das regiões Norte e Noroeste do Paraná, visando selecionar isolados fracos do Citrus tristeza virus com capacidade protetiva para pré-imunização. Os resultados apontaram sintomas fracos a moderados de caneluras...
A cross-protection program citrus tristeza disease, caused by Citrus tristeza virus (CTV) has been developed in the Parana State, to obtain Pera orange mother plants. Thus, 20 plants were selected in the orange commercial fields of the North and Northwest region. Comparisons between mild and severe recognized isolates were made. The results showed...
O presente trabalho teve como objetivo identificar 11 isolados de Guignardia citricarpa, agente causal da mancha preta dos citros (MPC), obtidos de frutas cítricas sintomáticas de diferentes regiões geográficas, por meio da PCR e caracterização morfofisiológica das estruturas propagativas, esporulação e crescimento micelial em diferentes meios de c...
O presente trabalho teve como objetivo identificar 11 isolados de Guignardia citricarpa, agente causal da mancha preta dos citros (MPC), obtidos de frutas cítricas sintomáticas de diferentes regiões geográficas, por meio da PCR e caracterização morfofisiológica das estruturas propagativas, esporulação e crescimento micelial emdiferentes meios de cu...
The sharpshooters of the families Cicadellidae and Cercopidae are vectors of Xylella fastidiosa (Wells), which causes citrus variegated chlorosis. This disease causes severe damage to the Paraná citrus industry, and it has been studied in Brazil since 1987, when it was discovered for the first time in the area of Colina, SP. The vectors transmit X....
RESUMO. As cigarrinhas das famílias Cicadellidae e Cercopidae são vetores da bactéria Xylella fastidiosa Wells, causadora da Clorose variegada dos citros. Esta doença traz sérios prejuízos à lavoura de citros no Noroeste Paranaense, e é estudada no Brasil desde 1987, quando foi descoberta pela primeira vez na região de Colina, São Paulo. Os vetores...
O presente trabalho teve como objetivo identificar 11 isolados de Guignardia citricarpa, agente causal da mancha preta dos citros (MPC), obtidos de frutas cítricas sintomáticas de diferentes regiões geográficas, por meio da PC R e caracterização morfofisiológica das estruturas propagativas, esporulação e crescimento micelial em diferentes meios de...
The citrus variegated chlorosis (CVC), an important disease of citrus in Brazil, is caused by the bacterium Xylella fastidiosa Wells et al. and transmitted by xylem-feeding sharpshooters (Hemiptera: Cicadellidae). This study evaluated the fluctuation of populations of species of sharpshooters belonging to the tribes Cicadellini and Proconiini, from...
A mancha preta ou pinta preta dos citros, causada por Guignardia citricarpa Kiely [Phyllosticta citricarpa (McAlp.) van der Aa.], foi registrada no Brasil pela primeira vez em 1980, no Rio de Janeiro, afetando pomares comerciais de mexerica do 'Rio', nos municípios de São Gonçalo e Itaboraí, na baixada fluminense. Em São Paulo a doença foi constata...
Spatio-temporal analysis of the citrus variegated chlorosis (CVC) in the Northwest of Parana, using PCR for detection of Xylella fastidiosa. Countless diseases affect the citriculture, as the citrus variegated chlorosis (CVC) which is caused by the bacteria Xylella fastidiosa. The aim of this work was to determine the space distribution of the dise...
resumO a clorose variegada dos citros (CvC) é uma das doenças mais graves que afetam a citricultura no brasil. tem como agente causal a bactéria Xylella fastidiosa subsp pauca, que é trans-mitida por cigarrinhas (Hemiptera, Cicadellidae) da subfamilia Cicadellinae. O objetivo deste estudo foi avaliar a abundância desses vetores nas variedades de la...
Use of spray-dried sugar cane yeast in pelleted diet or meal diet for
piglets in the initial phase. A trial was conducted using 144 male and female crossbred
piglets (48-to-69-day-old and 15.5kg of initial weight) to evaluate nutritionally and
economically sugar cane yeast (Saccharomyces sp.), dried by spray-dryer (SCYSD), in pelleted
or meal diet...
ABSTRACT. Use of rotative-roll-dried or spray dried sugar cane yeast in the feeding of piglets in the initial phase. A trial was undertaken using 144 male and female half-breed piglets (15.9 kg), with the objective to evaluate nutritionally and economically sugar cane yeast (Saccharomyces sp.), dried by spray-dryer (SCYSD) or by rotative roll dryer...
