Carlos Vay

Carlos Vay
Universidad de Buenos Aires | UBA · Faculty of Pharmacy and Biochemistry

Associate Professor, PhD

About

294
Publications
58,074
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4,004
Citations
Citations since 2017
74 Research Items
1616 Citations
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2017201820192020202120222023050100150200250300
2017201820192020202120222023050100150200250300
Introduction
Carlos Vay currently works at the Faculty of Pharmacy and Biochemistry, University of Buenos Aires. Carlos does research in Gynaecology, Pharmacy and Infectious Diseases. Their current project is 'Identificación de bacilos gram-positivos del género Actinomyces y otros relacionados. Impacto clínico'.

Publications

Publications (294)
Article
Resumen El absceso cerebral es una infección focal caracterizada por acumulación de pus en el parénquima cerebral; su diagnóstico es de urgencia debido a la alta mortalidad que acarrea. Presentamos tres casos de pacientes con abscesos cerebrales con foco otogénico de origen polimicrobiano, que presentaron en común el aislamiento de Actinomyces euro...
Preprint
Full-text available
Chemotherapy mistreatment is partially due to a lack of rapid and reliable tools to discriminate between sensitive and resistant phenotypes. In many cases, the resistance mechanism is not fully understood, impacting diagnostic tools' absence. This work aims to determine the capacity of MALDI-TOF-MS profiling to discriminate between chemotherapy-res...
Article
Full-text available
Sepsis has been called the graveyard of pharmaceutical companies due to the numerous failed clinical trials. The lack of tools to monitor the immunological status in sepsis constrains the development of therapies. Here, we evaluated a test based on whole plasma peptidome acquired by MALDI-TOF-mass spectrometer and machine-learning algorithms to dis...
Article
Resumen Desulfovibrio spp. son bacterias anaerobias estrictas, ubicuas en la naturaleza, que pueden formar parte del tracto gastrointestinal humano o animal, pero también son bacterias ambientales presentes en el suelo y en el agua. Pueden persistir de manera asintomática en el intestino o comportarse como patógenos oportunistas, asociados con bact...
Article
Resumen El empiema necessitatis (EN) constituye una muy rara complicación de un empiema pleural en el cual la infección se extiende a los tejidos blandos adyacentes. La etiología por anaerobios es muy infrecuente y se da en el curso de infecciones crónicas. Se presenta el primer caso de empiema necessitatis por Campylobacter rectus. La identificaci...
Article
Objective to describe two blaKPC-2 plasmids recovered from P. aeruginosa isolates belonging to the ST654 and ST235 high-risk clones, and to compare with complete sequences of blaKPC-2 harboring plasmids available in public databases. Methods Antimicrobial susceptibility was determined according to CLSI guidelines. Genomes were sequenced using an I...
Article
The spread of carbapenem-resistant Enterobacterales has raised concern in clinical settings due to the limited therapeutic options available. OXA-48-like enzymes are still sporadic in South America. The aim of this study was to characterize a multidrug-resistant Escherichia coli isolate from a hospitalized patient in Buenos Aires city. The isolate...
Article
Vibrio vulnificus is one of the most virulent Vibrio species known. It is a bacterium with universal distribution. The first case registered in Uruguay occurred in 2001 and, since then, several infections have occurred per year. Recently, in this country, V. vulnificus was responsible for a fatal soft tissue infection. Although no cases of human in...
Article
Resumen Dolosigranulum pigrum es un coco gram positivo, anaerobio facultativo, que forma parte de la microbiota oral y del tracto respiratorio superior. Aunque los reportes de infecciones por este microorganismo son escasos, se lo ha asociado a un amplio espectro de enfermedades infecciosas. Se describe el caso de un hombre adulto con un absceso co...
Article
The emergence of metallo-β-lactamase (MBL)-producing Enterobacterales , mainly New Delhi metallo-β-lactamase (NDM), represents a clinical threat due to the limited therapeutic alternatives. Aztreonam (AZT) is stable to MBLs, but most MBL-producing Enterobacterales isolates usually co-harbour other β-lactamases that confer resistance to AZT and, con...
Article
Full-text available
Bovine mastitis is a disease of dairy cattle prevalent throughout the world that causes alterations in the quality and composition of milk, compromising technological performance. Staphylococcus aureus is one of the most important pathogens that produce clinical, subclinical, and chronic mastitis. Biofilms are considered a virulence factor necessar...
Article
Peptostreptococcus anaerobius is a gram-positive anaerobic coccus (GPAC) found in the gastrointestinal and vaginal microbiota. The organism is mainly found in polymicrobial and scarcely in monobacterial infections such as prosthetic and native endocarditis. Anaerobic bacteria have rarely been reported as the cause of urinary tract infection (UTI)....
Article
Introduction An increase in recent years in the isolation of Vagococcus spp. is suggestive of emerging infection by this pathogen in our hospital. Methods Prospective, descriptive study. Period: July 2014–January 2019. Phenotypic identification of 15 isolates of Vagococcus spp. was performed by conventional biochemical tests, automated methodology...
Article
Full-text available
Therapeutic options for the treatment of infections by multidrug-resistant Acinetobacter baumannii strains are often limited. Minocycline (MIN) is an old antibiotic, with excellent activity against A. baumannii isolates, which can be administered orally. Currently, there is no single criterion regarding the breakpoints for MIN and A. baumannii. The...
Article
Full-text available
Immune responses at the boundary between the host and the world beyond are complex and mucosal tissue homeostasis relies on them. Obstructive sleep apnea (OSA) is a syndrome suffered by children with hypertrophied tonsils. We have previously demonstrated that these tonsils present a defective regulatory B cell (Breg) compartment. Here, we extend th...
Article
Full-text available
Salicylic acid (SAL) has recently been shown to induce biofilm formation in Staphylococcus aureus and to affect the expression of virulence factors. This study was aimed to investigate the effect of SAL on the regulatory agr system and its impact on S. aureus biofilm formation. The agr quorum-sensing system, which is a central regulator in S. aureu...
Article
Full-text available
Resumen Corynebacterium kroppenstedtii es un bacilo gram positivo no esporulado, inmóvil, fermentador de glucosa y lipofílico, que forma parte de la microbiota de la piel. En los últimos años se han comunicado numerosos aislamientos de esta especie, principalmente en infecciones mamarias, como abscesos y mastitis granulomatosa. Presentamos 4 casos...
Article
Full-text available
Corynebacterium spp. are Gram-positive rods that are recognized to cause opportunistic diseases under certain predisposing clinical conditions. Some species have been described in urinary tract infections. In this report we document a new episode of urinary tract infection caused by Corynebacterium phoceense and describe the whole-genome sequencing...
Preprint
Full-text available
Immune responses at the boundary between the host and the world beyond are complex and mucosal tissue homeostasis relies on them. Obstructive sleep apnoea (OSA) is a syndrome suffered by children with hypertrophied tonsils. We have previously demonstrated that these tonsils present a defective regulatory B cell (Breg) compartment. Here, we extend t...
Article
Full-text available
Actinomyces and related genera are grampositive bacilli, opportunistic pathogens, which have been mainly involved in endogenous infections. However, due to the complexity in identifying them for most clinical laboratories, there is scant knowledge about their real clinical significance. In this work, 166 isolates of 13 different species of Actinomy...
Preprint
Full-text available
Sepsis has been called "the graveyard of pharmaceutical companies" due to the numerous failed clinical trials. The lack of tools to monitor the immunological status in sepsis constrains the development of immunomodulatory therapies. Here, we evaluated a test based on whole plasma peptidome acquired in a MALDI-TOF-mass spectrometer used for bacteria...
Article
Full-text available
Coronavirus disease 2019, known as COVID-19, is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The early, sensitive and specific detection of SARS-CoV-2 virus is widely recognized as the critical point in responding to the ongoing outbreak. Currently, the diagnosis is based on molecular real time RT-PCR techniques, alth...
Article
A rapid colorimetric method, the Andrade screening antimicrobial test, was compared with the E-test method to detect ceftazidime/avibactam (CZA) resistance in carbapenem resistant Enterobacterales clinical isolates. A 106 non-duplicated isolates (86 susceptible and 20 resistant to CZA) were chosen for validation. The sensitivity and specificity wer...
Article
Resumen Introducción En los últimos años un aumento en la frecuencia de aislamiento de Vagococcus spp. estaría indicando la urgencia de la infección por este patógeno en nuestra institución. Métodos Estudio prospectivo y descriptivo que abarca el periodo de julio de 2014 a enero de 2019. La identificación fenotípica de 15 aislados de Vagococcus s...
Article
Full-text available
Introduction. The therapeutic options to treat Acinetobacter baumannii infections are very limited. Aim. Our aim was to evaluate the activity of sulbactam combined directly with avibactam or the ampicillin-sulbactam/ceftazidime-avibactam combination against extensively drug-resistant A. baumannii isolates. Methodology. Extensively drug-resistant A....
Article
Full-text available
Clostridium ramosum is an enteric anaerobic, endospore-forming, gram-positive rod with a low GC content that is rarely associated with disease in humans. We present a case of C. ramosum bacteraemia. To the best of our knowledge, this is the second case of C. ramosum bacteraemia in an elderly patient presenting with fever, abdominal pain and bilious...
Preprint
Full-text available
Coronavirus disease 2019 (COVID-19) is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The rapid, sensitive and specific diagnosis of SARS-CoV-2 by fast and unambiguous testing is widely recognized to be critical in responding to the ongoing outbreak. Since the current testing capacity of RT-PCR-based methods is being ch...
Article
Full-text available
Miasis is the infestation of man and animals by larvae of flies belonging to the order Diptera, suborder Cyclorrapha. Eighty percent of miasis in Argentina is caused by Cochliomyia hominivorax, a species that induces pronounced tissue invasion and destruction, and results in severe clinical forms. Because of the aggressiveness of its larvae, it is...
Article
Different MALDI-TOF MS databases were evaluated for the identification of Achromobacter species. The in-house and extended database generated in this study rendered more accurate identification (58/64 and 57/64 isolates, respectively) in comparison with the Bruker commercial database (42/64 isolates), especially in those infrequent species that are...
Article
Full-text available
Carbapenemase-producing Enterobacterales expressing OXA-48, KPC, NDM, VIM or IMP enzymes are increasingly reported worldwide. We have characterized LMB-1, a novel metallo-β-latamase (MBL) of Ambler class B3 from Citrobacter freundii 164 (Cf164) clinical isolate from Buenos Aires, Argentina. Cf164 displayed reduced susceptibility to carbapenems but...
Article
Full-text available
During the periods 2000-2004 and 2014-2015, Neisseria meningitidis was investigated in men who have sex with men, 1143 and 544 respectively, who consulted in the sexually-transmitted disease program. Prevalence, serogroup distribution and susceptibility to antibiotics were determined. Pharyngeal, rectal and urethral swabs were cultivated on selecti...
Article
Full-text available
Objective The aim of this study was to determine the capacity of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) to identify 155 HACEK clinical isolates and other fastidious or infrequently isolated Gram-negative rods ( e.g. , Actinobacillus , Capnocytophaga, Pasteurella, Neisseria, Moraxella, Dysgonomona...
Article
Abiotrophia defectiva, previamente conocida como variante nutricional de estreptococos forma parte de la microbiota normal de la cavidad oral, urogenital y tracto gastrointestinal. Se asocia con infecciones severas como bacteriemia, endocarditis, abscesos cerebrales, osteomielitis, artritis séptica y queratopatía del cristalino. Es un microorganism...
Article
Full-text available
The increasing use of polymyxins as last-resort drugs for managing infections by Acinetobacter baumannii has led to the emergence of resistance. This study aimed to determine the resistance mechanisms in Acinetobacter baumannii isolates with colistin MIC ≥ 4 mg/L and to relate the mechanisms of resistance with the difficulties in detecting them. Ab...
Article
Full-text available
A rapid colorimetric method, the Andrade Screening Antimicrobial Test (ASAT) was evaluated to detect colistin resistance in Enterobacteriales clinical isolates. The sensitivity and specificity were 90.7% and 100%, respectively. In 10/26 E. coli isolates the automatized method failed to detect the resistance, whereas the ASAT detected it accurately....
Article
Full-text available
Mass spectrometry has revolutionized the clinical microbiology field in America's and Europe's industrialized countries, for being a fast, reliable and inexpensive technique. Our study is based on the comparison of the performance of two commercial platforms, Microflex LT (Bruker Daltonics, Bremen, Germany) and Vitek MS (bioMérieux, Marcy l´Etoile,...
Article
Full-text available
In 2014, a novel species of Acinetobacter, strain A47, determined to be hospital-acquired was recovered from a single patient soft tissue sample following a traumatic accident. The complexity of the Acinetobacter genus has been established, and every year novel species are identified. However, specific features and virulence factors that allow memb...
Article
Cutibacterium avidum is a gram-positive anaerobic rod belonging to the cutaneous group of human bacteria with preferential colonization of sweat glands in moist areas. The microorganism rarely cause disease, generally delayed prosthetic joint infections (PJIs). We describe the second case of intraperitoneal abscess by C. avidum after an abdominal s...
Article
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Different phenotype-based techniques and molecular tools were used to describe the distribution of different Achromobacter species in patients with cystic fibrosis (CF) in Argentina, and to evaluate their antibiotic resistance profile. Phenotypic identification was performed by conventional biochemical tests, commercial galleries and MALDI-TOF MS....
Article
In this study, we identified specific carbapenemase-producing isolates applying an easy and rapid protocol for the detection of mature KPC-2 β-lactamase by MALDI-TOF MS from colony and positive blood culture bottles. In addition, we evaluated the correlation of the ~11,109 Da signal as a biomarker associated with KPC-2 production. A collection of 1...
Article
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The etiology leading to neonatal damage is multifactorial, being genital infections one of the causes. The objective of the study was to identify microorganisms of the maternal genital tract that are associated with neonatal damage, in order to prevent future perinatal complications. Seven hundred and eleven pregnant patients attended their prenata...
Article
MALDI-TOF (matrix assisted laser desorption ionization-time of flight) mass spectrometry (MS) proved to be a robust tool for the identification of numerous taxonomic groups. However, it has limitations. A key advantage of this technique is the flexibility for the incorporation of protein profiles of microorganisms not included in the commercial dat...
Article
Full-text available
Background Streptococcus pneumoniae (SP) is one of the most common pathogens of Community-Acquired Pneumonia (CAP), but recent reports suggest that its incidence may be declining in relation to the use of the conjugate 13-valent pneumococcal vaccine in children. We compared the result of the immunochromatographic SP urinary antigen test (SPUAT) and...
Article
Full-text available
The spread of antibiotic resistance is rapidly threatening the effectiveness of antibiotics in the clinical setting. Many infections are being caused by known and unknown pathogenic bacteria that are resistant to many or all antibiotics currently available. Empedobacter falsenii is a nosocomial pathogen that can cause human infections. E. falsenii...
Article
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The Southern green stinkbug (N. viridula) feeds on developing soybean seeds in spite of their strong defenses against herbivory, making this pest one of the most harmful to soybean crops. To test the hypothesis that midgut bacterial community allows stinkbugs to tolerate chemical defenses of soybean developing seeds, we identified and characterized...
Data
Bacterial communities in the midgut of Nezara viridula associated to the insects hosts. (PDF)
Data
Map of Argentina (a) and a zoom of central east Argentina (b) were 26 collecting events were performed during 2012–2014. Nezara viridula adults were handpicked from secondary hosts (light grey spots), Soybean (dark grey spots) or from under de bark of Eucalyptus trees (black spots). (PDF)
Data
(a) Cysteine protease activity of N. viridula V1-V4 midgut ventricles. Statistical differences are denoted by different letters. (b) Distribution of ARISA detected bacteria among N. viridula V1-V4 midgut ventricles. Bacterial ITS fragments appear as blue peaks and LIZ 1200 weight standard fragments appear as yellow peaks. On a black square are 748...
Data
Geographical placement of collecting sites. (PDF)
Data
Bacteria isolated in this work and those used to build phylogenetic trees of Enterococcus sp. (PDF)
Data
Bacteria isolated in this work and those used to build phylogenetic trees of Yokenella. (PDF)
Article
Full-text available
Members of the viridans group streptococci (VGS) are the cause of local and invasive infections. Due to the severity of these infections and taking into account that reports regarding epidemiological aspects are scarce, the aims of this work were the identification and the study of the antibiotic susceptibility profiles of the isolates recovered fr...
Article
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Purpose. The aim of this work was to investigate the presence of selected potential virulence factors, susceptibility and clonal relatedness among 63 Stenotrophomonas maltophilia isolates recovered from patients exposed to invasive devices in a university hospital in Argentina between January 2004 and August 2012. Methodology. Genetic relatedness w...
Article
Full-text available
Introduction: Although Corynebacterium species are part of the skin and mucous membranes microbiota, human infections caused by Corynebacterium spp. have been reported and the multidrug resistance pattern of the recovered isolates was emphasized. Due to the usefulness of disk diffusion in daily practice, the purpose of this work was to compare dis...
Article
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Introduction Corynebacterium coyleae is a Gram-stain-positive non-lipophilic coryneform rod first described in blood samples and pleural fluid. There is scarce information about the clinical relevance of C. coyleae and none on complicated urinary tract infections has been described so far. Case presentation A 36-year-old woman with a history of ch...
Article
Fast typing methods for third generation cephalosporin resistance mechanisms are needed to guide appropriate treatment and prevent potential dissemination events. In this study we used a novel short and fast methodology for the identification of CMY-2 in 50 well characterized clinical isolates of E. coli by matrix-assisted laser desorption/ionizati...
Article
Objectives: The aim of this study was to characterize OXA-258 variants and other features that may contribute to the carbapenem resistant in Achromobacter ruhlandii. Methods: Kinetic parameters for purified OXA-258a and OXA-258b were determined measuring the rate of hydrolysis of a representative group of antibiotics. Whole-genome shotgun sequen...
Article
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The association of Comamonas kerstersii with peritonitis resulting from perforated appendix and its isolation from a psoas abscess and pelvic peritonitis have previously been described by us. We present the first case of C. kerstersii urinary tract infection, broadening the spectrum of infections caused by this species.
Article
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Introduction Ignavigranum ruoffiae is an extremely rare cause of human infections. Case presentation An 83-year-old male with a painless, ten-day-old, erythematous skin abscess on his left flank, which had showed a purulent discharge for 48 h, was admitted to the Emergency service. He was treated with cephalexin, disinfection with Codex water and...
Article
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Clinically significant NDM-1 producing Acinetobacter schindleri has not been described yet in the literature. Here, we report the first case of bacteremia due to an A. schindleri strain, harboring blaNDM-1 recovered from an immunocompromised patient. Our report reinforces the fact that NDM-1 can be easily acquired by Acinetobacter species.
Article
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The association of Comamonas kerstersii with peritonitis secondary to the presence of perforated appendix has previously been described by our research team. In the present study, we describe the isolation of this microorganism from two forms of unusual presentations of C. kerstersii infection not previously described in the literature: localized i...
Article
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Bifidobacterium scardovii species consists of facultative anaerobic gram-positive rods whose growth is stimulated by CO2 and anaerobiosis. Exceptionally it has been associated with infections in humans. An elderly male patient with a urinary tract infection due to B. scardovii and Enterococcus faecalis is presented here; both microorganisms were is...
Poster
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Descripción de un caso de faringitis debida a C. diphtheriae no toxigénico
Poster
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The genus Bordetella including the three human pathogens Bordetella pertussis (Bp), Bordetella parapertussis (Bpp) and Bordetella bronchiseptica (Bb), which are considered the classical Bordetella species. Bp is strictly a human pathogen and is the etiologic agent of whooping cough, an acute respiratory disease that is particularly serious in child...
Article
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South American Camelids have an increasing relevance in local economies, worldwide. These animals are bred for their meat, fur and as companion and therapy animals. Thus, their sanitary status should be well-established. According to the OIE (World Organization for Animal Health), respiratory infections mainly produced by Pasteurella spp. have been...
Article
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The aim of this work is to study the dispersion of two ISs, ISCR2 and IS26, which are known to contribute in the acquisition of resistance determinants and genome plasticity, among a collection of hundred and seventy-four Acinetobacter spp. clinical isolates. PCR amplification reactions using total DNA were performed to search ISCR2, IS26, and diff...
Article
Full-text available
Objectives: Patterns of antimicrobial susceptibility in Actinomyces and related genera are very limited in the literature. Data of predominant susceptibility profiles could contribute to the establishment of an accurate empirical treatment. Methods: A total of 113 isolates from clinical samples were included in this study. Each isolate was ident...