Carlos Scrideli

• Doctor of Medicine, PhD
• Professor (Full) at University of São Paulo

Background Adrenocortical cancer (ACC) is rare and aggressive, with YAP1 overexpression associated with poor outcomes in pediatric patients. In this study, we investigated the mechanisms by which YAP1 drives ACC progression and explored it as a potential target therapy. Methods YAP1 expression and methylation in ACC were analyzed from pediatric an...

Disclosure: A.B. Arruda: None. R.P. da Silva-Júnior: None. M.F. Stecchini: None. J. Marrero-Gutiérrez: None. I. Cardinalli: None. T. Junqueira: None. C. Scrideli: None. C.A. Molina: None. T. Silvio: None. T. Silvio: None. F. Coeli-Lacchini: None. F.S. Ramalho: None. L. Ramalho: None. A.C. Moreira: None. S. Brandalise: None. A. Yunes: None. R.Z. Vên...

Disclosure: A.B. Arruda: None. R.P. da Silva-Júnior: None. M.F. Stecchini: None. J. Marrero-Gutiérrez: None. I. Cardinalli: None. T. Junqueira: None. C. Scrideli: None. C.A. Molina: None. T. Silvio: None. T. Silvio: None. F. Coeli-Lacchini: None. F.S. Ramalho: None. L. Ramalho: None. A.C. Moreira: None. S. Brandalise: None. A. Yunes: None. R.Z. Vên...

Human Pegivirus-1, typically regarded as a commensal virus, exhibits high prevalence in humans. Its frequency and impact on oncologic pediatric patients with febrile neutropenia (FN), a frequent chemotherapy complication, remains unexplored. In this study, we assessed HPgV-1 RNA prevalence in pediatric patients experiencing FN. Blood samples were c...

Pediatric adrenocortical tumors (ACT) are rare aggressive neoplasms with heterogeneous prognosis. MicroRNA (miRNA) signatures have been associated with cancer diagnosis, treatment response, and outcomes of several types of cancer. However, the role played by miRNAs in pediatric ACT has been poorly explored. In this study, we have evaluated the expr...

BACKGROUND Choroid plexus carcinoma (CPC) is a rare pediatric brain tumor occuring predominantly in infancy. It is commonly associated with the cancer predisposition Li-Fraumeni syndrome (LFS). Management of CPC patients with LFS is challenging and there is no accepted standard treatment. This multinational retrospective analysis aimed to describe...

Pediatric adrenocortical tumors (ACTs) are rare, highly heterogeneous neoplasms with limited therapeutic options, making the investigation of new targets with potential therapeutic or prognostic purposes urgent. The PRKAB2 gene produces one of the subunits of the AMP-activated protein kinase (AMPK) complex and has been associated with cancer. Howev...

Objective: To assess the prevalence of chronic neutropenia (CN) and the clinical profile of patients with CN aged up to 18 years, followed in the pediatric hematology, rheumatology, or immunology outpatient clinic of a tertiary medical center from May 1, 2018, to 30 April 2019. Methods: Retrospective observational study carried out by collecting...

Purpose Medulloblastomas are the most common primary malignant brain tumors in children. They are divided into molecular subgroups: WNT-activated, SHH-Activated, TP53 mutant or wild type, and non-WNT/non-SHH (Groups 3 and 4). WNT-activated medulloblastomas are usually caused by mutations in the CTNNB1 gene (85%–90%), and most remaining cases of CTN...

Groups (Grp) 3 and 4 are aggressive molecular subgroups of medulloblastoma (MB), with high rates of leptomeningeal dissemination. To date, there is still a paucity of biomarkers for these subtypes of MBs. In this study, we investigated the clinical significance and biological functions of Musashi-1 (MSI1) in Grp3 and Grp4-MBs. First, we assessed th...

Background: Advances in cancer treatment have caused the population of childhood cancer survivors (CCSs) to increase. CCSs are at risk of multiple therapy-related late effects (LEs). This study aims to evaluate the LE incidence and to assess how the body mass index (BMI) evolved in a CCS cohort from a Brazilian tertiary center. Methods: Retrospecti...

Groups (Grp) 3 and 4 are aggressive molecular subgroups of medulloblastoma (MB), with high rates of leptomeningeal dissemination. To date, there is still a paucity of biomarkers for these subtypes of MBs. In this study, we investigated the clinical significance and biological functions of Musashi-1 ( MSI1) in Grp3 and Grp4-MBs. First, we assessed t...

Members of the HDAC family are predictive biomarkers and regulate the tumorigenesis in several cancers. However, the role of these genes in the biology of intracranial ependymomas (EPNs) remains unexplored. Here, an analysis of eighteen HDACs genes in an EPN transcriptomic dataset, revealed significantly higher levels of HDAC4 in supratentorial ZFT...

Pediatric high-grade glioma (pHGG) is one of the most aggressive brain tumors. Treatment includes surgery, radiotherapy, chemotherapy, or combination therapy in children older than 3–5 years of age. These devastating tumors are influenced by the hypoxic microenvironment that coordinatively increases the expression of carbonic anhydrases (CA9 and CA...

Objetivos As neoplasias mieloides com predisposição germinativa foram incluídas como uma nova entidade clínica pela Organização Mundial de Saúde em 2016. A predisposição genética para LMA associada a mutação germinativa do CEBPA é de herança autossômica dominante, sendo todas variantes germinativas do tipo frameshift e na porção N-terminal. A detec...

Objetivo Apresentar a experiência com imunoterapia em linfomas pediátricos recidivados ou refratários à Quimioterapia (QT). Metodologia Estudo descritivo e retrospectivo de 3 casos admitidos no serviço de oncologia e hematologia pediátrica do HCFMRP-USP que utilizaram imunoterapia devido a linfomas refratários ou recidivados (2016 a 2021). Os dado...

Patients diagnosed with acute lymphoblastic leukemia (ALL) bearing t(4;11)/MLL-AF4 have aggressive clinical features, poor prognosis and there is an urgent need for new therapies to improve outcomes. Panobinostat (LBH589) has been identified as a potential therapeutic agent for ALL with t(4;11) and studies suggest that the antineoplastic effects ar...

Pediatric adrenocortical tumor (ACT) is a rare and aggressive neoplasm, with incidence in southern and southeastern Brazil 10–15 times higher than worldwide. Although microRNAs (miRNAs) have been reported to act as tumor suppressors or oncogenes in several cancers, the role of miR-149-3p in ACT remains unknown. In this study, we evaluated the expre...

Children diagnosed with adrenocortical tumors (pACT) have variable outcomes and, to date, the disease lacks robust prognostic biomarkers. The prognostic potential of tumor methylation has been demonstrated in several cancers. We aimed to evaluate the pACT methylation profile and its association with disease presentation and survival. In this cross-...

Groups (Grp) 3 and 4 are aggressive molecular subgroups of medulloblastoma (MB), with high rates of leptomeningeal dissemination. To date, there is still a paucity of biomarkers for these subtypes of MBs. The RNA-binding protein Musashi-1 ( MSI1 ) is a neural stem cell marker, characterized as a gene translation regulator and associated with high o...

Background Supratentorial RELA fusion (ST-RELA) ependymomas (EPNs) are resistant tumors without an approved chemotherapeutic treatment. Unfortunately, the molecular mechanisms that leads to chemoresistance traits of ST-RELA remains elusive. The aim of this study was to assess RELA fusion-dependent signaling modules, specifically the role of the Hed...

Patients diagnosed with acute lymphoblastic leukemia (ALL) bearing t(4;11)/MLL-AF4 have aggressive clinical features, poor prognosis and there’s an urgent need for new therapies to improve outcomes. Panobinostat (LBH589) has been identified as a potential therapeutic agent for ALL with t(4;11) and studies suggest that the antineoplastic effects is...

Medulloblastoma is the most common type of pediatric malignant primary brain tumor, and about one-third of patients die due to disease recurrence and most survivors suffer from long-term side effects. MB is clinically, genetically, and epigenetically heterogeneous and subdivided into at least four molecular subgroups: WNT, SHH, Group 3, and Group 4...

The Group 3 Medulloblastoma (Grp3-MB) is an aggressive molecular subtype with a high incidence of metastasis and deaths. In this study, were used an RNA sequencing data (RNA-Seq) from a Brazilian cohort of MBs to identify hub genes associated with the metastatic risk. Data validation were performed by using multiple large datasets from MBs (GSE8521...

Objective: Pediatric adrenocortical tumors (pACT) display complex genomic backgrounds, lacking robust prognostic markers and targeted therapeutic options. Vitamin D3 receptor (VDR) promoter hypermethylation and underexpression were reported in adrenocortical carcinomas from adult patients. We aimed to investigate VDR expression levels and methylati...

Pediatric adrenocortical tumors (ACT) are rare aggressive neoplasms with heterogeneous prognosis. Despite extensive efforts, identifying reliable prognostic factors for pediatric patients with ACT remains a challenge. MicroRNA (miRNA) signatures have been associated with cancer diagnosis, treatment response, and prognosis of several types of cancer...

Medulloblastoma (MB) is the most common malignant brain tumor in childhood. Currently, MB is assigned in four molecular subgroups (SHH, WNT, Group 3, and Group 4) and subtyped in 12 variants. The alpha subtype of the SHH subgroup bears TP53 mutation and is considered very high risk by the World Health Organization (WHO). In the current study, we ha...

Background: Medulloblastoma, a genetically heterogeneous tumor, is the most frequent malignant brain tumor in children. Although several studies have been carried out, the molecular mechanism underlying medulloblastoma tumorigenesis is not completely known. microRNA (miRNA) expression profiles have been associated with development, progression, and...

A extensão universitária tem ampliado seu campo de atuação junto ao Ensino e à Pesquisa nas instituições públicas e privadas. Com a finalidade de avaliar os pressupostos e metodologias na tríade ensino-pesquisa-extensão, esse relato de experiência teve como objetivo fazer uma reflexão sobre nossa didática como extensionistas na relação professor-al...

Pediatric adrenocortical tumors (ACT) are rare aggressive neoplasms with heterogeneous prognosis. Despite extensive efforts, identifying reliable prognostic factors for pediatric patients with ACT remains a challenge. MicroRNA (miRNA) signatures have been associated with cancer diagnosis, treatment response, and prognosis of several types of cancer...

Group (Grp) 3 and 4 are the deadliest molecular subgroups of medulloblastoma (MB) with high rates of leptomeningeal dissemination. Yet, there is still a paucity biomarkers for these MBs subtypes. Previous results from our group have identified Musashi-1 (MSI1) overexpressed in Grp3/Grp4-MBs tumor samples and in D283 Med (Grp3/Grp4-MB) cells. In can...

The Group 3 Medulloblastoma ( Grp3-MB ) is an aggressive molecular subtype with a high incidence of metastasis and deaths. In this study, were used an RNA sequencing data ( RNA-Seq ) from a Brazinian cohort of MBs to identify hub genes associated with the metastatic risk. Data validation were performed by using multiple large datasets from MBs (GSE...

Glioblastomas (GBMs), the most common and lethal primary brain tumor, show inherent infiltrative nature and high molecular heterogeneity that make complete surgical resection unfeasible and unresponsive to conventional adjuvant therapy. Due to their fast growth rate even under hypoxic and acidic conditions, GBM cells can conserve the intracellular...

Adrenocortical carcinoma (ACC) is a rare, but highly aggressive cancer of the adrenal cortex withgenerally poor prognosis. Despite being rare, completely resected ACCs present high risk of recurrence. Musashi-2 (MSI2) has recently been recognized as potential prognostic biomarker and therapeutic target in many cancers. However, no studies have eval...

Although ependymoma (EPN) molecular subgroups have been well established by integrated high-throughput platforms, low- and middle-income countries still need low-cost techniques to promptly classify these molecular subtypes. Here, we applied low-cost methods to classify EPNs from a Brazilian cohort with 60 pediatric EPN patients. Fusion transcripts...

Pediatric adrenocortical tumors (pACTs) display complex genomic background and lack robust prognostic biomarkers. However, pACT methylation profiling was recently associated with patient prognosis. In order to evaluate whether tumor DNA methylation is associated with patient prognosis, we studied a Brazilian cohort of pACT in which most of the pati...

Medulloblastoma (MB) is classified into 4 molecular subgroups. Groups 3 and 4 MB compose a chal-lenging MB category regarding diagnosis and treatment approaches. Musashi-1 (MSI1) is a highly conserved RNA binding protein (RBP)and your overexpression is strongly associated with poor prognosis in several human cancers; yet, its role in MB is still il...

Medulloblastoma (MB) is a neuroepithelial tumor from cerebellum and it is currently subdivided in 4 molecular subgroups, WNT, SHH, Group 3 and Group 4. SKP2 has been associatedto progression and development of some cancers, but, there is no studies in MB. The aim of this study was evaluating the SKP2 gene expression profile in MB molecular subgroup...

Adrenocortical tumors (ACT) are rare neoplasms in children with aggressive behavior and incidence in Brazil 10x higher than worldwide. Although previous studies have suggested the coparticipation of different signaling pathways during adrenal development, little is known about the crosstalk between Wnt and TGF-β pathways in ACT. Thus, the aim of th...

Medulloblastoma is classified into four molecular subgroups with very different prognosis and is closely related to dysregulations of the signaling pathways that control normal embryonic development. The aim of this study was to identify hubgenes from overlapping of differentially expressed genes with each set of genes according to the subgroup. Hu...

Introduction: Glioblastoma (GBM) is the most common subtype and lethal brain tumor in the human species. The treatment of GBM remains a major challenge for clinicians since these aggressive brain tumors are highly resistant to radio- and chemotherapy. The poor prognosis of GBM patients is in part due to the presence of a heterogeneous population of...

Pediatric malignant adrenocortical tumor s(ACT) are extremely agressive and rare. Sequencing of tumor samples is a well-used approach that has enabled the generation of molecular classifications with clinical implications for adult patients. However, transcriptomic studies are still scarce for childhood ACT. Thus, the aim of this study was to ident...

SHOC2 scaffold protein has been mainly related to oncogenic ERK signaling through the RAS-SHOC2-PP1 phosphatase complex. In leukemic cells however, SHOC2 upregulation has been previously related to an increased 5-year event-free survival of pediatric pre-B acute lymphoid leukemia, suggesting that SHOC2 could be a potential prognostic marker. To add...

Glioblastoma (GBM) is the most common primary malignant neoplasm of the central nervous system and, despite the standard therapy; the patients' prognoses remain dismal. The miRNA expression profiles have been associated with patient prognosis, suggesting that they may be helpful for tumor diagnosis and classification as well as predictive of tumor...

Background: Medulloblastoma (MB) is the most common malignant brain tumor in childhood. Despite improvement in current treatment, children still suffer from long-term sequelae resulting from standard care therapy. World Health Organization considers MB as 4 distinct entities: SHH, WNT, Group 3, and Group 4. The most aggressive variant of SHH MB is...

Introduction: Recently we found that RELA ependymomas (EPN) demonstrate inappropriate activation of the Hedgehog (Hh) pathway. However, how this activation is modulated remains to be investigated. Primary cilia are essential to positive and negative modulation of Hh signaling and changes in ciliation seem to be linked to Hh drug resistance in pedia...

RELA-fused supratentorial (ST) ependymoma (EPN) is an aggressive subgroup with poor prognosis. Considering the putative role of Notch signaling in the maintenance of the cancer stem cells (CSC) phenotype in RELA-fused EPN, we investigated the expression of Notch pathway and its target genes in this subgroup. We also evaluated the effects of two Not...

Medulloblastoma (MB) is the most frequent malignant brain tumor in children and it is subgrouped into 4 entities (SHH,WNT, Group 3, and Group 4). Molecular pathways involved in these different subgroups still are evolving and can be ofclinical relevance to therapy. The YAP1-CTGF axis is known to regulate cell proliferation, differentiation, and cel...

We evaluated the potential effects of ATO in different pediatric SHH-MB cell lines (ONS-76: TP53-wild type; DAOY and UW402: TP53-mutated). MB cell lines molecular subgroup was confirmed and TP53 mutations were validated. Cell viability, clonogenicity and apoptosis were evaluated after ATO treatment at different concentrations (1–16 µM) alone or com...

Aberrant expression of long non-coding RNAs (lncRNAs) has been detected in several types of cancer, including acute lymphoblastic leukemia (ALL), but lncRNA mapped on transcribed ultraconserved regions (T-UCRs) are little explored. The T-UCRs uc.112, uc.122, uc.160 and uc.262 were evaluated by quantitative real-time PCR in bone marrow samples from...

Long non-coding RNA (lncRNA) aberrant expression have been found in several types of cancer, including acute lymphoblastic leukemia (ALL), but lncRNA mapped in transcribed ultraconserved regions (T-UCRs) are little explored. The T-UCRs uc.112, uc.122, uc.160 and uc.262 were evaluated in pediatric ALL and uc.112 expression was higher in T-ALL compar...

Medulloblastoma (MB) of Groups 3 and 4 are genetically less characterized and do not have a specific deregulated signaling pathway to date. These 2 MB subgroups constitute a challenge in relation to diagnostic and treatment approaches. It has been described that the Notch pathway is deregulated in the MB 3 and 4 subgroups. The Notch pathway is resp...

Musashi (MSI) 1 and 2 are RNAbinding proteins that regulate several cellular functions and recently have emerged as regulators of cancer development. However, their role in adrenocortical tumors (ACT) remain unknown. ACT are rare in children, buts its incidence is particularly higher in Brazil. Currently, there are no reliable biomarkers to predict...

Background Ependymoma (EPN) is the third most common childhood cancer of the central nervous system. RELA fusion-positive EPN accounts for approximately 70% of all childhood supratentorial tumors and shows the worst prognosis among the supratentorial EPNs. TP53 mutation is infrequent in RELA fusions EPNs. In the population from the Southern region...

V018 SIOP19-1341 LYON, FRANCE, OCTOBER 23–26, 2019 Medulloblastoma (MB) is classified into 4 molecular subgroups: WNT, SHH, Group-3 and 4. Groups 3 and 4 are less characterized genetically and compose a challenging MB stratum regarding diagnosis and treatment approaches. Musashi-1 (MSI1) is a highly conserved RBP protein; its hyperexpression is s...

Câncer é um assunto frequente na mídia e no cotidiano de muitos estudantes, uma vez que ainda representa um problema de saúde pública com elevado apelo emocional e social. A falta do senso crítico no processo de ensino e aprendizagem do câncer é evidenciada pela defasagem de informações e conceitos científicos básicos durante a formação escolar. Ne...

Osteosarcoma (OS) is the most common primary malignant bone tumor with two peaks of incidence, in early adolescence and the elderly. Patients affected with this malignancy often present metastatic disease at diagnosis, and despite multimodality therapy, survival has not improved substantially over the past 3 decades. Recently, miR-138-5p, proposed...

Next-generation sequencing platforms are routinely used for molecular assignment due to their high impact for risk stratification and prognosis in medulloblastomas. Yet, low and middle-income countries still lack an accurate cost-effective platform to perform this allocation. TaqMan Low Density array (TLDA) assay was performed using a set of 20 gen...

Purposes Pilocytic astrocytoma (PA) is a low-grade neoplasm frequently found in childhood. PA is characterized by slow growth and a relatively good prognosis. Genetic mechanisms such as activation of MAPK, BRAF gene deregulation and neurofibromatosis type 1 (NF1) syndrome have been associated with PA development. Epigenetic signature and miRNA expr...

Background Overall survival of Ewing sarcoma (EWS) remains poor and less than 30% of patients with metastatic or recurrent disease survive despite current treatments. Thus, there is a constant search for new biomarkers for diagnosis, prognosis and prediction of therapy. Numerous studies have reported the abnormal expression of miR-708-5p in tumors...

Medulloblastoma (MB) is the most common malignant childhood brain tumor. MB is currently classified into four molecular subgroups (Wnt, Shh, Group 3, and Group 4). The wingless (Wnt) pathway is responsible for embryonic development and is deregulated in MB. We analyzed the activation of the Wnt pathway in MB cell lines and its correlation with the...

Long non-coding RNA (lncRNA) aberrant expression have been found in several types of cancer, including acute lymphoblastic leukemia (ALL), but lncRNA mapped in transcribed ultraconserved regions (T-UCRs) are little explored. The T-UCRs uc.112, uc.122, uc.160 and uc.262 were evaluated in pediatric ALL and uc.112 expression was higher in T-ALL compar...

We present a case of an infant who developed pro-B acute lymphoblastic leukemia with a rare and complex MLL-translocation. Cytogenetic analysis of bone marrow cells at diagnosis showed a 46,XY,t(X;11)(p11.2;q23)[13]/46,XY[7] karyotype. Fluorescence in situ hybridization analysis using a break apart specific probes showed a split in the MLL gene. Lo...

Purpose: Acute lymphoblastic leukemia (ALL) is the most common type of cancer in children, and despite the high rate of cure (over 80%) it still has a big impact on morbidity and mortality. The Transducin-like enhancer of split 1 (TLE1), a transcriptional corepressor, has been described as dysregulated and recently emerged as a tumor marker in sev...

Introduction: Medulloblastoma (MB) is an embryonal tumour that originates from genetic deregulation of cerebellar developmental pathways and is classified into 4 molecular subgroups: SHH, WNT, group 3, and group 4. Hydroxymethylation levels progressively increases during cerebellum development suggesting a possibility of deregulation in MB pathoge...

Introduction: Glioblastoma (GBM) is the most common malignant primary brain tumor affecting adults. In pediatric patients, GBM exhibits genetic variations distinct from those identified in the adult GBM phenotype. This tumor exhibits complex genetic changes leading to malignant progression and resistance to standard therapies including radiotherap...

Introduction Medulloblastoma (MB) is the most common brain tumour in childhood. Despite improvement in current treatment, childrens still suffers with the long-term sequelae due to multimodal therapy. Current consensus consider MB as 4 distinct entities: MB SHH, WNT, Group 3 and Group 4 and are subclassified in 12 subtypes. The most agressive subty...

Introduction Ependymoma (EPN) is the third most common childhood cancer of the central nervous system. RELA subgroup EPN accounting for approximately 70% of all childhood supratentorial tumours and shows the worst prognosis among the supratentorial EPNs. Due to the low efficacy of the chemotherapy the treatment options for this tumour are limited....

Introduction Medulloblastoma (MB) is an embryonic neuroepithelial tumour and important embryonic development pathways, such as WNT, Sonic Hedgehog (SHH) and Notch, are dysregulated on this cancer. The Notch pathway is important to cell differentiation and proliferation and it has a role in initiation and progression of MB regulating downstream effe...

Material and methods: Cell survival was analyzed through XTT®, clonogenicity and caspase-3 activation assays were also studied, and drug interactions analyzed through a nonlinear regression of a sigmoid dose response model. Sensibilization to radiation was assessed through enhancement ratio calculation. Results: Mild effects on the viability of...

Over the last decade, the rho-associated kinases and several metastasis-associated microRNAs have emerged as important contributors of tumor invasion. However, despite prominence, our understanding of their involvement in the metastatic potential of Ewing Sarcoma (EWS) is incomplete. The expression profiles of ROCK1 or ROCK2 and miR-124-3p, miR-138...

Dysregulated mitotic kinases have frequently been associated with cancer. Changes in their expression might result from diverse mechanisms including avoidance of the tight regulation exerted by miRNAs. Herein we show that miR-10b* is downregulated in osteosarcoma samples and demonstrate its correlation with PLK1, PLK4, BUB1, and BUBR1, which are st...

Purpose: NF-κB is a transcription factor involved in the transcriptional regulation of a large number of genes related to tumorigenesis in several cancer cell types, and it's inhibition have been related to anticancer effect. DHMEQ (Dehydroxymethylepoxyquinomicin) is a compound that blocks the translocation of NF-κB from the cytoplasm to the nucle...