Carlos Sanchez-Huertas

Carlos Sanchez-Huertas
Instituto de Neurociencias

PhD

About

22
Publications
1,232
Reads
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365
Citations
Additional affiliations
May 2019 - present
Centro de Investigación Príncipe Felipe
Position
  • PostDoc Position
December 2015 - present
French National Centre for Scientific Research
Position
  • PostDoc Position
October 2010 - November 2015
IRB Barcelona Institute for Research in Biomedicine
Position
  • PostDoc Position

Publications

Publications (22)
Article
Full-text available
During the establishment of neural circuitry axons often need to cover long distances to reach remote targets. The stereotyped navigation of these axons defines the connectivity between brain regions and cellular subtypes. This chemotrophic guidance process mostly relies on the spatio-temporal expression patterns of extracellular proteins and the s...
Article
Full-text available
Microtubule (MT) plus-end tracking proteins (+TIPs) are central players in the coordination between the MT and actin cytoskeletons in growth cones (GCs) during axon guidance. The +TIP Navigator-1 (NAV1) is expressed in the developing nervous system, yet its neuronal functions remain poorly elucidated. Here, we report that NAV1 controls the dynamics...
Article
Full-text available
Organization of microtubules into ordered arrays is best understood in mitotic systems, but remains poorly characterized in postmitotic cells such as neurons. By analyzing the cycling cell microtubule cytoskeleton proteome through expression profiling and targeted RNAi screening for candidates with roles in neurons, we have identified the mitotic k...
Article
Full-text available
Neurons display a highly polarized microtubule network that mediates trafficking throughout the extensive cytoplasm and is crucial for neuronal differentiation and function. In newborn migrating neurons, the microtubule network is organized by the centrosome. During neuron maturation, however, the centrosome gradually loses this activity, and how m...
Data
(related to Fig. 3a, b) Mitochondrial motility in axons is impaired in γ-tubulin depleted neurons. Hippocampal cultures were infected with the indicated lentivirus, transfected with the mitochondrial reporter MitoDsRed at 4 DIV and the axons were imaged 24 hours later by time-lapse microscopy. The soma of the neurons is located at the left side of...
Data
(related to Fig. 1g, h) Depletion of γ-tubulin reduces microtubule nucleation in the soma of hippocampal neurons. Cultured neurons were infected with lentivirus carrying shRNAs and transfected at 4 DIV with the reporter EB3-Tomato during 24 hours before live-imaging analysis.
Data
(related to Fig. 1i, j) Reduction of γ-tubulin levels impairs microtubule nucleation in axons. Transduced hippocampal neurons were transfected with EB3-tomato reporter at 4 DIV and the axons were imaged by time-lapse microscopy. The soma of the neurons is located at the left side of the videos.
Data
(related to Fig. 7a-d) The augmin complex is required for the uniform polarity of microtubules in hippocampal axons. Neurons were infected with the indicated lentivirus and transfected with EB3-Tomato 24 hours before imaging the axons by time-lapse microscopy. The soma of the neurons is located at the left side of the videos.
Data
(related to Fig. 8e-g) Overactivating γTuRC by expression of the 51-100 aa conserved region of CDK5RAP2 promotes mixed microtubule polarity in axons. Hippocampal neurons were co-transfected at 3 DIV with EGFP or EGFP-CDK5RAP2 51-100 together with the EB3-Tomato reporter and axons were imaged 24 hours later. The soma of the neurons is located at the...
Chapter
A multitude of protein activities contribute to the organization of cell type and cell cycle-specific microtubule arrays. One key factor is the γ-tubulin ring complex (γTuRC), a microtubule nucleator that determines where and when new microtubules are generated. Other proteins interact with newly formed or existing microtubules to promote microtubu...
Article
Full-text available
CEP63 is a centrosomal protein that facilitates centriole duplication and is regulated by the DNA damage response. Mutations in CEP63 cause Seckel syndrome, a human disease characterized by microcephaly and dwarfism. Here we demonstrate that Cep63 deficient mice recapitulate Seckel syndrome pathology. The attrition of neural progenitor cells involv...
Article
Microtubules mediate important cellular processes by forming highly ordered arrays. Organization of these networks is achieved by nucleating and anchoring microtubules at centrosomes and other structures collectively known as microtubule-organizing centers (MTOCs). However, the diverse microtubule configurations found in different cell types may no...
Article
Full-text available
In the cerebral cortex, the functional output of projection neurons is fine-tuned by inhibitory neurons present in the network, which use γ-aminobutyric acid (GABA) as their main neurotransmitter. Previous studies have suggested that the expression levels of the rate-limiting GABA synthetic enzyme, GAD65, depend on brain derived neurotrophic factor...

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