Carlos Pardo-Pastor

Carlos Pardo-Pastor
University Pompeu Fabra | UPF · Department of Experimental and Health Sciences

PhD

About

15
Publications
2,952
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519
Citations
Additional affiliations
March 2015 - July 2015
Johns Hopkins University
Position
  • Visiting scholar
January 2014 - present
University Pompeu Fabra
Position
  • PhD Student
September 2012 - July 2014
University Pompeu Fabra
Position
  • Research Assistant

Publications

Publications (15)
Article
Full-text available
Cell extrusion is a mechanism of cell elimination that is used by organisms as diverse as sponges, nematodes, insects and mammals1–3. During extrusion, a cell detaches from a layer of surrounding cells while maintaining the continuity of that layer⁴. Vertebrate epithelial tissues primarily eliminate cells by extrusion, and the dysregulation of cell...
Preprint
Full-text available
Cell extrusion is a process of cell elimination in which a cell is squeezed out from its tissue of origin. Extrusion occurs in organisms as diverse as sponges, nematodes, insects, fish and mammals. Defective extrusion is linked to many epithelial disorders, including cancer. Despite broad occurrence, cell-intrinsic triggers of extrusion conserved a...
Article
Full-text available
Actin polymerization and assembly into stress fibers (SFs) is central to many cellular processes. However, how SFs form in response to the mechanical interaction of cells with their environment is not fully understood. Here we have identified Piezo2 mechanosensitive cationic channel as a transducer of environmental physical cues into mechanobiologi...
Article
Full-text available
Cyclin O (encoded by CCNO) is a member of the cyclin family with regulatory functions in ciliogenesis and apoptosis. Homozygous CCNO mutations have been identified in human patients with Reduced Generation of Multiple Motile Cilia (RGMC) and conditional inactivation of Ccno in the mouse recapitulates some of the pathologies associated with the huma...
Article
Full-text available
Ewing sarcoma (ES) is an aggressive tumor defined by EWSR1 gene fusions that behave as an oncogene. Here we demonstrate that RING1B is highly expressed in primary ES tumors, and its expression is independent of the fusion oncogene. RING1B-depleted ES cells display an expression profile enriched in genes functionally involved in hematological develo...
Article
Full-text available
Cells adopt distinct signaling pathways to optimize cell locomotion in different physical microenvironments. However, the underlying mechanism that enables cells to sense and respond to physical confinement is unknown. Using microfabricated devices and substrate-printing methods along with FRET-based biosensors, we report that, as cells transition...
Article
Full-text available
Galindo-Villegas, J., A. Montalban-Arques, S. Liarte, S. de Oliveira, C. Pardo-Pastor, F. Rubio-Moscardo, J. Meseguer, M. A. Valverde, and V. Mulero. 2016. TRPV4-mediated detection of hyposmotic stress by skin keratinocytes activates developmental immunity. J . Immunol . 196: [738–749][1]. A
Article
Full-text available
As an organism is exposed to pathogens during very early development, specific defense mechanisms must take effect. In this study, we used a germ-free zebrafish embryo model to show that osmotic stress regulates the activation of immunity and host protection in newly hatched embryos. Mechanistically, skin keratinocytes were responsible for both sen...
Article
Functional transient receptor potential (TRP) channels result from the assembly of four subunits. Here, we show an interaction between the pre-S1, TRP, and the ankyrin repeat domain (ARD)-S1 linker domains of TRPV1 and TRPV4 that is essential for proper channel assembly. Neutralization of TRPV4 pre-S1 K462 resulted in protein retention in the ER, d...
Article
Full-text available
Calcium signaling participates in different cellular processes leading to cell migration. TRPV4, a non-selective cation channel that responds to mechano-osmotic stimulation and heat, is also involved in cell migration. However, the mechanistic involvement of TRPV4 in cell migration is currently unknown. We now report that expression of the mutant c...
Article
The widely distributed TRPV4 cationic channel participates in the transduction of both physical (osmotic, mechanical, and heat) and chemical (endogenous, plant-derived, and synthetic ligands) stimuli. In this chapter we will review TRPV4 expression, biophysics, structure, regulation, and interacting partners as well as physiological and pathologica...
Article
Full-text available
Most transient receptor potential (TRP) channels are regulated by phosphatidylinositol-4,5-biphosphate (PIP2), although the structural rearrangements occurring on PIP2 binding are currently far from clear. Here we report that activation of the TRP vanilloid 4 (TRPV4) channel by hypotonic and heat stimuli requires PIP2 binding to and rearrangement o...

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