Carlos Ezio Garciamendez-Mijares

• Researcher at Harvard Medical School

Significance This work reports the development of a physiologically relevant human alveolar lung-on-a-chip model, composed of a three-dimensional (3D) porous hydrogel made of gelatin methacryloyl (GelMA) featuring an inverse opal structure, bonded to a compartmentalized chip device that provides air–liquid interface and cyclic breathing motions. Si...

The use of bioprinting as a powerful tool for tissue and organ fabrication has been a promising development in the field of biomedicine, offering unprecedented versatility in the fabrication of biologically and physiologically relevant constructs. Even though there are a plethora of commercial bioprinters available in the market, most of them are o...

Light-based vat-polymerization bioprinting enables computer-aided patterning of 3D cell-laden structures in a point-by-point, layer-by-layer or volumetric manner, using vat (vats) filled with photoactivatable bioresin (bioresins). This collection of technologies — divided by their modes of operation into stereolithography, digital light processing...

Bioprinters show great promise as enablers of regenerative medicine and other biomedical engineering applications. In this work, we present a flexible and cost-effective design for a do-it-yourself bioprinter capable of printing/bioprinting gelatin methacryloyl (GelMA) and Pluronic constructs at flow rates of 0.05-0.1 mL/min and effective resolutio...

With the rapid development and popularization of additive manufacturing, different technologies, including, but not limited to, extrusion-, droplet-, and vat-photopolymerization-based fabrication techniques, have emerged that have allowed tremendous progress in three-dimensional (3D) printing in the past decades. Bioprinting, typically using living...

Electrical stimulation of existing three-dimensional bioprinted tissues to alter tissue activities is typically associated with wired delivery, invasive electrode placement, and potential cell damage, minimizing its efficacy in cardiac modulation. Here, we report an optoelectronically active scaffold based on printed gelatin methacryloyl embedded w...

To mimic physiological microenvironments in organ-on-a-chip systems, physiologically relevant parameters are required to precisely access drug metabolism. Oxygen level is a critical microenvironmental parameter to maintain cellular or tissue functions and modulate their behaviors. Current organ-on-a-chip setups are oftentimes subjected to the ambie...

Droplet‐based bioprinting has shown remarkable potential in tissue engineering and regenerative medicine. However, it requires bioinks with low viscosities, which makes it challenging to create complex 3D structures and spatially pattern them with different materials. This study introduces a novel approach to bioprinting sophisticated volumetric ob...

Decellularized extracellular matrix (dECM)‐based hydrogels are widely applied to additive biomanufacturing strategies for relevant applications. The extracellular matrix components and growth factors of dECM play crucial roles in cell adhesion, growth, and differentiation. However, the generally poor mechanical properties and printability have rema...

Volumetric printing, an emerging additive manufacturing technique, builds objects with enhanced printing speed and surface quality by forgoing the stepwise ink-renewal step. Existing volumetric printing techniques almost exclusively rely on light energy to trigger photopolymerization in transparent inks, limiting material choices and build sizes. W...

The human body relies on modular assembly for realizing its functions. Here the development of a bioinspired cryo(bio)printing‐based method is reported to fabricate shelf‐ready, storable modular scaffolds toward scalable tissue assembly. The mechanism lies in that the cryo(bio)printed modular scaffolds are first assembled into the final hierarchy i...

Developing a reproducible and secure supply of customizable control tissues that standardizes for the cell type, tissue architecture, and preanalytics of interest for usage in applications including diagnostic, prognostic, and predictive assays, is critical for improving our patient care and welfare. The conventionally adopted control tissues direc...

Three-dimensional (3D) printing is an emerging technique that has shown promising success in engineering human tissues in recent years. Further development of vat-photopolymerization printing modalities has significantly enhanced the complexity level for 3D printing of various functional structures and components. Similarly, the development of micr...

While there has been considerable success in the three-dimensional bioprinting of relatively large standalone filamentous tissues, the fabrication of solid fibers with ultrafine diameters or those cannular featuring ultrathin walls remains a particular challenge. Here, an enabling strategy for (bio)printing of solid and hollow fibers whose size ran...

Volumetric additive manufacturing (VAM) enables fast photopolymerization of three-dimensional constructs by illuminating dynamically evolving light patterns in the entire build volume. However, the lack of bioinks suitable for VAM is a critical limitation. This study reports rapid volumetric (bio)printing of pristine, unmodified silk-based (silk se...

Three‐dimensional (3D) bioprinting is driving significant innovations in biomedicine over recent years. Under certain scenarios such as in intraoperative bioprinting, the bioinks used should exhibit not only cyto/biocompatibility but also adhesiveness in wet conditions. Herein, an adhesive bioink composed of gelatin methacryloyl, gelatin, methacryl...

Digital light processing bioprinting favors biofabrication of tissues with improved structural complexity. However, soft-tissue fabrication with this method remains a challenge to balance the physical performances of the bioinks for high-fidelity bioprinting and suitable microenvironments for the encapsulated cells to thrive. Here, we propose a mol...

It is well‐known that tissue engineering scaffolds that feature highly interconnected and size‐adjustable micropores are oftentimes desired to promote cellular viability, motility, and functions. Unfortunately, the ability of precise control over the microporous structures within bioinks in a cytocompatible manner for applications in 3D bioprinting...

The architecture of the colloidal photonic crystals (CPCs) is of paramount importance to their functionality and applications. Nevertheless, the realization of CPCs with arbitrarily designed, volumetrically sophisticated structures at the macroscale remains challenging. In this work, a printable CPC ink was developed. By combining this ink with a d...

3D Cryo‐Bioprinting A vertical 3D cryo‐bioprinting technique is reported by Yu Shrike Zhang and co‐workers in article number 2108931, which concurrently allows the user to create freestanding filamentous structures containing interconnected, anisotropic microchannels featuring gradient sizes aligned in the vertical direction, also associated with e...

Due to poor mechanical properties of many hydrogel bioinks, conventional three-dimensional (3D) extrusion bioprinting is usually conducted based on the X-Y plane, where the deposited layers are stacked in the Z direction with or without the support of prior layers. Herein, we report a technique taking advantage of a cryoprotected bioink to enable d...

Thrombosis in the circulation system can lead to major myocardial infarction and cardiovascular deaths. Understanding thrombosis formation is necessary for developing safe and effective treatments. In this work, using digital light processing (DLP)-based 3D printing, we fabricated sophisticated in vitro models of blood vessels with internal microch...

Bioprinting In article number 2107038, Yu Shrike Zhang and co-workers develop a (bio)printing system based on Digital Light Processing to utilize the integration of a microfluidic mixer for the generation of either continual or discrete gradients of desired (bio)inks in real time. As such, the precisely controlled gradients are composable on-the-fl...

Recapitulation of complex tissues signifies a remarkable challenge and, to date, only few approaches have emerged that can efficiently reconstruct necessary gradients in three-dimensional (3D) constructs. This is true even though mimicry of these gradients is of great importance to establish the functionality of engineered tissues and devices. Here...

Although various (bio)fabrication technologies have achieved revolutionary progress in the past decades, engineered constructs still fall short of expectations owing to their inability to attain precisely designable functions. Shrinkable and expandable (bio)materials feature unique characteristics leading to size‐/shape‐shifting and thus have exhib...