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Publications (83)
A simplified freezing process was developed and successfully applied to embryogenic calluses of three sugarcane commercial hybrids (Saccharum sp. cv. CP 5243, C 91-301 and C 1051-73). To obtain optimal survival, the calluses were pretreated with a cryoprotective solution containing 10% DMSO and 0.3 to 0.75M sucrose. For freezing, the samples were i...
The most important operational expense during wastewater treatment is electricity for pumping and aeration. Therefore, this work evaluated operational parameters and contaminant removal efficiency of a microbial fuel cell stack system (MFCSS) that uses no electricity. This system consists of (i) septic tank primary treatment, (ii) chamber for secon...
ARTIFICIAL SEED SUGAR CANE PATENT
Huanglongbing (HLB) constitutes the most destructive disease of citrus worldwide, yet no established efficient management measures exist for it. Brassinosteroids, a family of plant steroidal compounds, are essential for plant growth, development and stress tolerance. As a possible control strategy for HLB, epibrassinolide was applied to as a foliar...
Sea lice source was similar. In all cases the glucose was consumed almost completely, although in the fed batch system itself of strategy used. are marine pathogen in recent decades in the salmon industry more expanded. These parasites cause skin lesions, which may cause osmotic imbalance and secondary infections. Losses for involvement at over 305...
Avian influenza virus H5N1 has caused extensive damage worldwide among poultry and humans. Effective expression systems are needed for the production of viral proteins required for monitoring this devastating disease. The present study deals with the establishment of a stable expression system for the hemagglutinin H5 (HAH5) of avian influenza viru...
The present invention describes a method for the stimulation of the natural defense and the induction of resistance to diseases in plants by simultaneous activation of genes related to the route of the salicylic acid, the jasmonic acid/ethylene and the hypersensitive response. The invention also includes the use of brassinosteroids for the preventi...
Transgenic crops expressing Bt toxins have substantial benefits for growers in terms of reduced synthetic insecticide inputs, area-wide pest management and yield. This valuable technology depends upon delaying the evolution of resistance. The ‘high dose/refuge strategy’, in which a refuge of non-Bt plants is planted in close proximity to the Bt cro...
In teleost fish, secretion of the growth hormone (GH) is regulated by several hypothalamic factors that are influenced by the physiological state of the animal. GH in fish is involved in many physiological processes that are not overtly growth-related such as: saltwater osmoregulation, antifreeze protein synthesis, and the regulation of sexual matu...
In teleost fish, secretion of the growth hormone (GH) is regulated by several hypothalamic factors that are influenced by the physiological state of the animal. GH in fish is involved in many physiological processes that are not overtly growth-related such as: saltwater osmoregulation, antifreeze protein synthesis, and the regulation of sexual matu...
RESUMEN Desde abril del 2004 se inició la extensión del bionematicida HeberNem en todo el país, hasta lograr en el presente la generalización de este producto en los cultivos protegidos de hortalizas (tomate, melón, pepino y pimiento), donde se ha demostrado que su Efectividad Técnica puede ser del 70 al 95% en el control de fitonematodos parásitos...
RESUMEN Los resultados de las pruebas toxicológicas y ecotoxicológicas realizadas al bionematicida HeberNem y su agente activo la cepa bacteriana Tsukamurella paurometabola C-924, demostraron su inocuidad al hombre, los animales y al medio ambiente. Se obtuvieron licencias y permisos de seguridad biológica que posibilitaron la aplicación de HeberNe...
The expression and production of pharmaceutical, industrial and veterinary proteins in plants is an attractive approach. These expression hosts bear an enormous production potential in terms of volume, easy processing of the starting material, and safety of the final product due to the lack of pathogens able to infect animal and human cells. Antibo...
The present invention reveals a nucleic acid sequence from Nicotiana megalosiphon encoding for an anti-pathogenic protein. The invention comprises the use of this nucleic acid molecule in transgenic plants of agricultural interest to confer resistance to pathogens. The invention also includes a bioproduct that comprises this anti-pathogenic protein...
The present invention reveals a nucleic acid sequence from Nicotiana megalosiphon encoding for an anti-pathogenic protein. The invention comprises the use of this nucleic acid molecule in transgenic plants of agricultural interest to confer resistance to pathogens. The invention also includes a bioproduct that comprises this anti-pathogenic protein...
Genetically engineered plants are used as protein production platform to manufacture biopharmaceuticals with high quality (e.g., posttranslational modification such as glycosylation and dimerization). Also the creation of plant-based expression systems can be achieved quickly and reliably within only weeks. On top, due to the intrinsic fast growth...
The expression and production of pharmaceutical, industrial and veterinary proteins in plants is an attractive approach. These expression hosts bear an enormous production potential in terms of volume, easy processing of the starting material, and safety of the fi nal product due to the lack of pathogens able to infect animal and human cells. Antib...
About 80% of the currently more than 200 biopharmaceuticals were approved after
2000, and more than 10 of those reached blockbuster status (>US$ 1 billion per
annum) in 2004 already. All together, with the highest growth rates (compound
annual growth rate of 20%) within the entire pharma market, biopharmaceutical
sales in 2006 were about US$ 60 bil...
The highly pathogenic H5N1 avian influenza virus (AIV) has caused important economic losses and public health problems, mainly in Asia, Africa and Europe. Counteracting this virus by vaccination of poultry is crucial. In our study, recombinant antigens based on the fusion of the extracellular segments of both molecules, hemagglutinin H5 of AIV and...
The highly pathogenic H5N1 avian infl uenza virus (AIV) has caused important economic losses and public health problems, mainly in Asia, Africa and Europe. Counteracting this virus by vaccination of poultry is crucial. In our study, recombinant antigens based on the fusion of the extracellular segments of both molecules, hemagglutinin H5 of AIV and...
This invention relates to pesticide and anti parasitic compositions
for the control of pests, diseases and parasites attacking
plants and animals. The compositions include, at least one
chitinolytic agent or a chitinolytic activity-inducing agent,
and sulfide or a sulfide-producing agent from microorganisms
or chemical compounds, wherein the chitin...
Bacterial levan [β(2,6)–linked polyfructan] has potential applications in the food, bio-energetic, medical, pharmaceutical,
and other industries. The lack of technically and economically feasible large-scale production systems limits
the commercial exploitation of levan. Gluconacetobacter diazotrophicus secretes a levansucrase (LsdA, EC 2.4.1.10)
t...
The nematodes constitute one of the most phyto-pathogenic species in agriculture, using chemical and biological control agents. The Tsukamurella paurometabola is a bacterial strain with possibilities of being used as a nematicide; thus, it is necessary to evaluate the effect of its administration in lab animals with the objective of estimating its...
Fungi and oomycete-borne plant disease constitute the largest limiting factor for crop productivity and yields both in Cuba and worldwide. Vast amounts of fungicidal chemicals have to be spent every year for their control, despite considerable environmental damage and the risk such chemicals pose for human health. Searching for biotechnological dis...
World Health Organization has a great concern about the spreading of avian influenza virus H5N1. To counteract its massive spread, poultry vaccination is highly recommended together with biosecurity measures. In our study, a recombinant vaccine candidate based on the fusion of extracellular segments of hemagglutinin (HA) H5 of avian influenza virus...
This research was conducted with the aim of controlling the tick Riphicephalus (Boophilus) microplus through Herber biogar immunogen in cattle in the town of Guanare, Portuguesa, Venezuela. 28 units were investigated with a mass of 1 776 cattle breeding animals (Holstein × Cebú) dual purpose were taken two treatments in a completely randomized desi...
La presente investigación se realizó con el objetivo de controlar la garrapata
Riphicephalus (Boophilus) microplus por medio del inmunógeno Herber biogar
en bovinos en el municipio de Guanare, Portuguesa, Venezuela. Se
investigaron 28 unidades con una masa bovina de 1 776 animales mestizos
(Holstein x Cebú) de doble propósito y se conformaron dos t...
La presente investigación se realizó con el objetivo de controlar la garrapata
Riphicephalus (Boophilus) microplus por medio del inmunógeno Herber biogar
en bovinos en el municipio de Guanare, Portuguesa, Venezuela. Se
investigaron 28 unidades con una masa bovina de 1 776 animales mestizos
(Holstein x Cebú) de doble propósito y se conformaron dos t...
Biological control of nematodes is a valuable alternative to the use of chemical nematicides in agriculture, because of the high negative impact generated by such chemicals in agroecosystems. From the Gram positive bacterium Tsukamurella paurometabola C-924, the bionematicide HeberNem-L™ has been developed, which is presented as a liquid formulatio...
Growth hormone (GH) is a single chain polypeptide of approximately 22 kDa, produced by the pituitary gland and with pleiotropic functions among vertebrates. It mainly regulates body growth, being also involved in reproduction, immunity and osmoregulation in teleost fish. In order to obtain the tilapia (Oreochromis hornorum) growth hormone (tiGH) in...
La bacteria Tsukamurella paurometabola cepa C-924, en una formulación líquida (HeberNem-L), se registró como nematicida biológico con el permiso no. 001/2007 (del órgano de Registro Nacional de Sanidad Vegetal), t. 4, folio 1364. El objetivo de este trabajo es mostrar los resultados del uso de HeberNem en distintos sistemas productivos. El área tra...
Agrobacterium tumefaciens technology is the battle horse for tomato genetic transformation. However, tomato varieties with low regeneration capacity are very difficult to transform. In the past, tomato transformation through Agrobacterium infection was focused on varieties capable of high regeneration yield, while successful transformation of low r...
E2 is the major envelope glycoprotein present on the outer surface of the classical swine fever virus (CSFV). It is exposed as a homodimer originated by disulfide linkage and represents an important target for the induction of neutralizing immune responses against the viral infection. The E2his glycoprotein nucleotide sequence used in this work con...
The nematocidal agent, Tsukamurella paurometabola C-924, was cultured in a 300 l bioreactor. Spray-dried formulations of this microorganism were prepared using sucrose. At an outlet temperature 62 degrees C, survival rates between 12 and 85% were reached with sucrose up to 10% (w/w). The stability study of the powders showed that the best storage c...
Antibodies have been one of the proteins widely expressed in tobacco plants for pharmaceutical purposes, which demand contaminant free preparations. Rubisco constitutes 40-60% of tobacco leaf soluble proteins; therefore it is the major potential protein contaminant of plantibodies, while mycotoxins are toxic compounds that could be introduced durin...
Regulatory agencies such as the FDA require the structure and amino acid sequence icharacterization of recombinant monoclonal antibodies (MAbs) to grant marketing approval. Characterizing such complex, inherently heterogeneous molecules is a significant analytical challenge that requires a broad array of physicochemical tests. Mass spectrometry (MS...
Within a short period of time, the teaching and popularization of Biotechnology has become a need not only for the academic world, but for society as well. The advances in this field have repercussions within the scientific community, as well as politicians, businessmen, manufacturers, lawyers, journalists and the general population alike. At the s...
Biological control of nematodes is a valuable alternative to the use of chemical pesticides in agricultural practices. Tsukamurella paurometabola C-924 is a microorganism whose nematocidic action has been reported. This bacterial strain was isolated from soils of Cuba as a good candidate for the biological control of phytonematodes and its action m...
The use of transgenic plants for the production of pharmaceutical compounds has received increasing attention in the last few years. However, many technological and regulatory issues regarding the practical exploitation of this alternative system of production remain to be solved; a situation that explains the lack of commercial products derived fr...
The area of land viable for agriculture is diminishing each year due to topsoil erosion, loss of soil fertility, water insecurity and climatic change, factors which limit productivity and threaten crop yields. The changing climate will exaggerate the existing differences between developed and developing countries and may also see pests and diseases...
Boophilus microplus has developed resistance against a range of chemical acaricides which has stimulated the development of alternative methods such as vaccination against ticks. In Cuba, the Bm86-based recombinant vaccine Gavac has been successfully used in a number of controlled laboratory and field trials in cattle against B. microplus. In this...
This paper provides an evaluation of a plant-derived HBsAg-specific antibody in the immunopurification of the recombinant HBsAg for vaccine purposes. This plant-derived antibody was obtained from different batches of 100-200kg of tobacco leaves and coupled to Sepharose CL-4B with high efficiency. The plant-derived antibody immunoaffinity matrix pur...
The application of bioengineering to plants for production of biological products for human and animal use has expanded in recent years. The reasons for this expansion are several and include advances in the technology for novel production systems and the need for very large quantities of therapeutic proteins. The process of growing pharmaceutical...
Temporary immersion bioreactors are an efficient tool for plant mass propagation because they increase multiplication rate
and plant quality. Little knowledge is available on the ecosystem and physiological behavior of plantlets when using this
new culture technique. In order to evaluate the effects of the conditions on physiological change of pine...
logró aislar, identificar y determinar una nueva cepa nematicida: Corynebacterium paurometabolum cepa C-924,
que resultó el más eficiente biocontrolador de altas poblaciones de fitonemátodos, entre ellos Meloidogyne incognita.
Se demostró que el mecanismo de acción de esta bacteria se debe a las quitinasas y al sulfuro de hidrógeno que
produce de m...
Se desarrollaron metodologías de trabajo in vitro, que permitieron estudiar 158 cepas de bacterias, con ello se
logró aislar, identificar y determinar una nueva cepa nematicida: Corynebacterium paurometabolum cepa C-924,
que resultó el más eficiente biocontrolador de altas poblaciones de fitonemátodos, entre ellos Meloidogyne incognita.
Se demostró...
The temporary immersion bioreactor has been found to be an important tool for sugarcane micropropagation, allowing higher
shoot formation rates and cost reduction. This research was conducted to demonstrate the agricultural value of temporary immersion
bioreactor-derived sugarcane plants. The experiment was carried out for about 2 yr to study the f...
Sugarcane shoot formation was followed using a temporary immersion system. Plant fresh weight, plant dry weight, shoot number
and phenolic excretion to the culture medium were recorded during shoot formation. Shoot number increased for 30 days of culture
but formation of new shoots was greatly reduced from 31 to 40 days. Phenolic excretion also inc...
In this paper, we describe a simplified cryopreservation protocol and its application to embryogenic calluses of three commercial varieties of sugarcane. We also present data on the effect of extended cryopreserved storage duration on the survival and plantlet production of calluses of one sugarcane variety.
A procedure for the mass propagation of pineapple plants (Ananas comosus L. Merr) using a temporary immersion technique is described. This procedure involved three distinct phases in the automated
temporary immersion system: shooting, bud differentiation and elongation. To establish this protocol, we used in vitro shoots
obtained from established l...
A simplified freezing process was developed and successfully applied to embryogenic calluses of three sugarcane commercial hybrids (Saccharum sp. cv. CP 5243, C 91-301 and C 1051-73). To obtain optimal survival, the calluses were pretreated with a cryoprotective solution containing 10% DMSO and 0.3 to 0.75M sucrose. For freezing, the samples were i...
Pineapple mealybug wilt (PMW) was first described in Hawaii in 1910 and has since spread wherever pineapple is cultivated. PMW is currently one of the most important field diseases of pineapple in Cuba, causing up to 40% crop loss. Leaves of plants affected with PMW turn bronze-red and lose turgidity, and the tips curve down. Root system size is de...
A new protocol was established for sugarcane cv. C-1051-73 shoot formation in a temporary immersion system. The two-step protocol
involves shoot formation in 50 ml of culture medium per explant and 1.0 mg l-1 paclobutrazol for 30 days followed by shoot
elongation by exposure to 1.0 mg l-1 gibberellic acid for 15 days. The multiplication rate was do...
Synthetic seed technology may be of value in breeding programs and allow the propagation of many elite genotype-derived plants in a short time. In this work, a range of artificial endosperm treatments of Cleopatra tangerine zygotic embryos were evaluated for suitability for encapsulation of somatic embryos. Different complexing ions in the form of...
A simplified freezing process was developed and successfully applied to embryogenic calluses of three sugarcane commercial hybrids (Saccharum sp. cv. CP 5243, C 91-301 and C 1051-73). To obtain optimal survival, the calluses were pretreated with a cryoprotective solution containing 10% DMSO and 0.3 to 0.75M sucrose. For freezing, the samples were i...
Plant regeneration from cultured immature inflorescence segments (35 mm) of sugarcane (Saccharum sp) var. CP 5243 was obtained via somatic embryogenesis. Embryogenic callus culture was initiated on MS medium supplemented with 2,4-D (13.5 µM) over 30 days. The callus was subcultured every 1520 days on MS medium supplemented with 2,4-D (4.5 µM), ar...
Different concentrations of dicamba were evaluated for the formation of callus from the base leaves obtained of in vitro growth plantlets (var. Smooth Cayenne and Red Spanish). From these explants were obtained callus of slow growing, hard, nodular and pale yellow, similar to embryogenetic callus described by other authors. Plant regeneration in th...
Different media for in vitro germination of pineapple hybrid seeds were tested. They had been obtained from a genetic improvement programme carried out in Ciego de Avila. The germination dynamics, conversion percentage, and the plantlet fresh weight were analyzed. The distilled water and salts + MS vitamin media were more advisable in the analyzed...
MS medium with 0.1 mg/L BA and 0.5 mg/L was adequate to shooting from stem segments. Cefotaxime (750 mg/L) controlled Agrobacterium tumefaciens growth in this medium and did not affect shooting statistically. An 8.9 % of GUS+ shoots was obtained following this procedure.
The purpose of this workshop was to provide research information and a forum for discussion on control of citrus tristeza closterovirus and its vector Toxoptera citricida.
SE ESTUDIO LA COMPOSICION QUIMICA DE LA TESTA,EL TEGMEN Y LOS COTILEDONES DE SEMILLAS BOTANICAS DE MANDARINA CLEOPATRA (CITRUS RESHNI HORT EX TAN),PROCEDENTES DE FRUTOS MADUROS.LOS CARBOHIDRATOS FUERON LOS COMPUESTOS DE MAYOR CONCENTRACION:EN LOS COTILEDONES Y EL TEGMEN,LA SACAROSA (14.08 Y 42.43 mg.g-1 DE MASA SECA,RESPECTIVAMENTE)Y EN LA TESTA,LA...
Los experimentos de endospermo artificial realizados con embriones naturales de mandarina Cleopatra (Citrus reshni Hort ex Tan) demostraron que la concentración de ácido abscísico (ABA) de 10-6 M provocó retraso en la germinación y conversión de los embriones naturales; los aminoácidos aceleraron el proceso de conversión mientras que el manitol lo...
With the aim to increase citrus fllowering under tropical conditions, two formulations of 2-chloroethyl phosphonic acid (Ethrel
and Flordimex), Alar (succinic acid-2, 2-dimethyl hydrazide) and Cycocel (2-chloroethyl-trimethyl ammonium chloride) were
applied during the flower induction period (December) to young “Valencia” orange trees at concentrat...
There are several world fums devoted to chemical trade; however, there are just a few standards set on this concern. Given its name, code anckriteria denoting product quality, as well as package shape and its specific capacity,.then the price and definitions of one or other firm are different. An underdeveloped, blocked country like Cuba should fig...
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