Carlos Angulo

Centro de Investigaciones Biológicas del Noroeste S.C. | CIBNOR

Totoaba (Totoaba macdonaldi), an ancient fish endemic to the Gulf of California (Sea of Cortez), has faced significant challenges in recent decades. Since 1990, several serious issues have been raised regarding conservation, fisheries, and sustainable aquaculture. In the search for solutions for sustainable Totoaba aquaculture in Mexico, research f...

Aquaculture is the fastest-growing food industry worldwide because it allows faster intensive production in a limited space and short time. However, the trade-off of this production scheme has led to infectious disease outbreaks that harm food production with economic impacts. Immunostimulants have entered the industry to fight against diseases by...

Cannabis sativa plant have been widely investigated for their specific compounds with medicinal properties. Those bioactive compounds have exerted preventive and curative effects on non-communicable and infectious diseases. However, C. sativa extracts have been barely investigated although they constitute an affordable option to treat human disease...

Enteric diseases by pathogenic organisms are one of the leading causes of death worldwide, particularly in low‐income countries. Despite antibiotics, access to clean water and vaccination are the most economically affordable options to prevent those infections and their health consequences. Vaccines, such as those approved for rotavirus and cholera...

Ticks are obligate hematophagous ectoparasites that affect animals, and some of them transmit a wide range of pathogens including viruses, bacteria, and protozoa to both animals and humans. Several vaccines have shown immunogenicity and protective efficacy against ticks in animal models and definitive hosts. After several decades on anti-tick vacci...

In recent decades, interest has been aroused worldwide in the use of silicon in nutrition; however, information on its effect on nutrition and metabolism of fish is limited. The objective of the research was to evaluate the effect of dietary supplementation with organic silicon on the growth performance, blood biochemistry, digestive enzymes, morph...

Citation: Granados-Amores, A.; Campa-Córdova, Á.I.; Acosta-Salmón, H.; Angulo, C.; Zenteno-Savín, T.; Rodríguez-Jaramillo, C.; Saucedo, P.E. Antioxidant Activity and Oxidative Damage Associated with Seeding Surgery for Pearl Culture in the Winged Pearl Oyster Pteria sterna. Antioxidants 2024, 13, 723. https:// Abstract: To evaluate the antioxidant...

Microalgal emergence is a promising platform with two‐decade historical background for producing vaccines and biopharmaceuticals. During that period, microalgal‐based vaccines have reported successful production for various diseases. Thus, species selection is important for genetic transformation and delivery methods that have been developed. Altho...

The ‘sacred leaf’ or “Hoja Santa” (Piper auritum Kunth) has a great value for Mexican culture and has gained popularity worldwide for its excellent properties from culinary to remedies. To contribute to its heritage, in this project we proposed the green synthesis of silver oxide nanoparticles (Ag2O NPs) using an extract of “Hoja Santa” (Piper auri...

The synthesis of gold nanoparticles (AuNPs) by bottom-up methods, such as redox reactions using amino acids and gold salts, has turned out to be a novel method for obtaining nanoparticles due to the reducing properties of these biomolecules and the ability to give the nanoparticle peculiar physicochemical characteristics for its biological applicat...

¿Qué es una vacuna? Un líquido que contiene un patógeno muerto, debilitado o partes de él que se aplica para protegernos de una enfermedad. Entonces, ¿qué es una nanovacuna? Es una vacuna que usa una nanopartícula para transportarla dentro del cuerpo. Pero claro, una duda sería ¿qué es una nanopartícula? Es una sustancia, cosa o material de tamaño...

Antimicrobial resistance is an important health concern globally, and probiotics are considered an alternative to minimize it. The present study examined the in vitro probiotic characteristics and in vivo immunomodulatory potential of Bacillus sp. 62A — an extremophile bacterium. Bacillus sp. 62A was evaluated in vitro for its cytotoxicity, hemolyt...

This study analyzed the antibiotic resistance of pathogenic bacteria from poultry produced in Mexico. Antibiotic resistance to pathogenic bacteria was evaluated by agar diffusion method using Enrofloxacin, Oxytetracycline, Sulfamethoxazole-Trimethoprim, and Streptomycin. The nine bacteria were sensible to Sulfamethoxazole-Trimethoprim, indicating i...

The aquaculture due to the high cost and continued shortage of animal protein sources, the development of alternative plant protein sources has been one of the main challenges to establish sustainable aquaculture as economically viable. Objective of this investigation was to determine the chemical composition and effect of Tithonia diversifolia on...

Schizochytrium sp. is a unicellular heterotrophic marine protist, with global distribution used at an industrial scale for saturated fatty acid and polyunsaturated fatty acid production. Diverse methodologies for the genetic transformation of Schizochytrium sp. have been developed to express heterologous proteins, such as through Agrobacterium tume...

Moringa oleifera is one of the most promising plants in aquaculture because it improves the health status, zootechnical parameters and resistance against diseases. This research evaluates the physicochemical, antioxidant values of spray-dried Moringa oleifera seed extract microencapsulates obtained at 140 and 180 °C with whey protein concentrate (W...

Chagas disease—caused by the parasite Trypanosoma cruzi—is a neglected tropical disease for which available drugs are not fully effective in the chronic stage and a vaccine is not available yet. Microalgae represent a promising platform for the production and oral delivery of low-cost vaccines. Herein, we report a vaccine prototype against T. cruzi...

Around 1.7 million people die annually due to enteric infections, which are mainly caused by ETEC (Enterotoxigenic E. coli), V. cholera, V. parahaemolyticus, and Salmonella. There are currently licensed vaccines against cholera and salmonella, whose distribution is chain-dependent making difficult to efficiently distribute them in poor countries. I...

The growing antibiotic resistance and low-efficient vaccines make searching for alternatives a need to fight infectious diseases in newborn calves. Thus, trained immunity could be used as a tool to optimize immune response against a wide range of pathogens. Although β-glucans have shown to induce trained immunity, it has not been demonstrated in bo...

p> Background . The live weight (LW) and morphometric variables of sheep are useful characteristics to generate adequate criteria for genetic improvement and conservation of Creole animal resources. Objective . To establish morphological relationships and through them predict LW of females and males in Creole sheep of the Oaxacan Mixteca, Mexico. M...

In recent decades, microalgal biotechnology has become one of the most valuable sources of functional feed additives. Many microalgae, such as Chlorella sp., Tetraselmis sp., Navicula sp., Phaeodactylum tricornutum, Porphyridium cruentum, Nannochloropsis gaditana, Dunaliella salina, Lobosphaera sp. and Schizochytrium sp., have been considered valua...

El dengue es una infección viral que prevalece en regiones tropicales y es causada por uno de cuatro serotipos del virus del dengue denominados DENV1, DENV2, DENV3 y DENV4 transmitidos al hombre, principal hospedero del virus, a través de la picadura de mosquitos del género Aedes. Un inadecuado control del mosquito y la infraestructura de salud def...

This study aimed to examine the effect of Debaryomyces hansenii CBS 8339 on innate immune responses in mice. Thirty BALB/c mice were randomly treated with phosphate buffered saline (PBS) (control) and two D. hansenii (Dh) doses: Dh 10ˆ6 CFU (colony forming units) and Dh 10ˆ8 CFU daily for 15 days. Spleen, blood, and gut samples were taken on days 7...

Antibiotic bacterial resistant is a huge concern worldwide and probiotics offer an alternative to mitigate it. This study explores Cystobasidium benthicum LR192 as possible probiotic through microbiological and immunological analyses in mouse model. C. benthicum LR192 was isolated from lichens in a hyperarid environment in Baja California Sur, Mexi...

Los probióticos son bichos diminutos cuyo consumo genera efectos benéficos en la salud. De hecho, la palabra probiótico significa 'pro-vida'. Algunas levaduras, como la utilizada para hacer pan o cerveza, pueden ser probióticos para animales y humanos. Desde hace algunos años, los estudios con la leva-dura Debaryomyces hansenii han demostrado que é...

Cacti" are rich sources of phytochemicals with antioxidant activity, and their use is mainly focused on infusions in traditional medicine in Mexico. This study characterizes the chemical compounds found in Cylindropuntia cholla root by gas chromatography coupled to mass spectrometry (GC-MS) and determines the total content of polyphenols and flavon...

Administration of immunostimulants in fish is a preventive method to combat infections. A wide variety of these biological molecules exist, among which one of the yeast wall compounds stands out for its different biological activities. The β-glucan that forms the structural part of yeast is capable of generating immune activity in fish by cell rece...

Cuantificación de polifenoles y flavonoides en hierba de la flecha (Sebastiania bilocularis) y su efecto tóxico para cabras durante las estaciones del año en Baja California Sur Abstract Arrow grass (Sebastiania bilocularis) is a native plant of southwest of México and grows from San Ignacio to Los Cabos in Baja California Sur and is characterized...

MINCLE is a C‐lectin receptor mainly expressed in macrophages to recognize carbohydrates enhanced by the innate immune response in mammals and fish. Toxin A (ToxA) from Vibrio parahaemolyticus N16 was evaluated as an antigenic protein; it was observed to induce IgM production, increase cytokine gene expression, and offer immunoprotection against ba...

Vaccines have saved millions of humans and animals from deadly diseases. Many vaccines are still under development to fight against lethal diseases. Indeed, subunit vaccines are a versatile approach with several advantageous attributes, but they lack strong immunogenicity. Nanotechnology is an avenue to vaccine development because nanoparticles may...

Nanovaccine development is a growing research field in which the development of new carriers and bioconjugation approaches is a priority. In this sense, this report describes for the first time, the development of a novel conjugate that consists of gold nanoparticles (AuNPs) obtained by a one-step synthesis using an immunogenic peptide of the Lipop...

The stressing effect of stocking density and rearing time was assessed on shrimp Penaeus vannamei reared intensively in floating cages. Juvenile shrimp were stocked in 9 m2 cages at 200, 250, and 300 ind m-2 for a 25-day grow-out period. Total soluble protein concentration, superoxide dismutase (SOD), and catalase (CAT) activities were used as stre...

Nucleobases, nucleosides and nucleotides can act as chemical markers and immunnostimulants. Ultra-high-performance liquid chromatography with electrospray ionization and quadrupole time of flight-mass spectrometry was used to determine bioactive compounds from different yeast species isolated from extremal marine environments, Yarrowia lipolytica,...

Antibiotic usage to control infectious diseases in shrimp aquaculture has led to serious problems on antimicrobial resistance. An alternative to mitigate this issue is the use of probiotics, which can be easily administered by feed and water. This study examines immunomodulatory and protective effects of the marine yeasts Debaryomyces hansenii CBS8...

Invertebrates' immune defense mechanisms play a critical role in pathogen recognition and elimination. De novo assembly and annotation of the Argopecten ventricosus transcriptome were performed for the immune-related gene identification. Scallops (height: 4.4 cm) were challenged with inactivated Vibrio parahaemolyticus IPNGS16. The RNA from differe...

Fusarium spp. damages the roots of crops, its control is with synthetic fungicides, however, marine actinomycetes can be an option to the use of agrochemicals. The objective of this work was the identification and morphological characterization of marine actinomycetes as antagonists to Fusarium solani (Mart.) Sacc. Fusarium spp. was isolated from d...

Excessive use of antibiotics has led to an increase of pathogenic bacteria with multiple antibiotic resistance. Hypersaline and hyperthermal environments promote the development of several microorganisms that can potentially act as immunostimulants. Thus, the aim of this study was bioprospecting marine bacteria from these environments using mouse l...

Trained immunity is a biological concept that has been demonstrated in different animal species, including human beings. Evidences indicate that innate immune cells can be trained and have a “memory”. Under this concept, studies have shown that a first stimulus can potentiate immune responses upon a second one or protect upon homologous or heterolo...

This study was conducted to investigate the effects of dietary incorporation of a medicinal plant (Mexican Chenopodium ambrosioides L.) on growth parameters, cholesterol level, immune and antioxidant parameters in serum and antioxidant enzyme gene expressions in intestine of Pacific red snapper before and upon Aeromonas hydrophila challenge. Fish w...

Vaccines for human use have conventionally been developed by the production of (1) microbial pathogens in eggs or mammalian cells that are then inactivated, or (2) by the production of pathogen proteins in mammalian and insect cells that are purified for vaccine formulation, as well as, more recently, (3) by using RNA or DNA fragments from pathogen...

p> Background. Successful immunomodulatory effects of yeast structural and secretory bioactive compounds have been demonstrated on several animal species. Likewise, studies have shown the ability of Debaryomyces hansenii to stimulate both immune and antioxidant responses on fish, crustaceans, mollusks, and recently, goats. Objective. To analyze the...

Yarrowia lipolytica has been widely used in food industry but scarcely explored as probiotics. Thus, the aims of this study were to characterize in vitro the probiotic potential, antioxidant capacity, and antimicrobial activity of the marine yeast Y. lipolytica D-1 and N-6 strains. Dietary administration effect was evaluated in vivo on immunologica...

Turnera diffusa commonly called Damiana is a wild shrub traditionally used as aphrodisiac, diuretic, and for cancer and diabetes treatments. Its extracts are rich in flavonoid, phenolic, and terpenoid compounds that have been reported as potential reductors for metal ions. In this sense, this study reports a novel and facile method for green gold n...

Microalgae come to age as a vaccines production host and delivery vehicle. Antigens have been selected based on their protective effects or by in silico tools. The design has been focused on the type of antigen and its subcellular localization. Currently, several genetic engineering tools have been developed because microalgal cells as production h...

The yeast Yarrowia lipolytica has been industrially adopted for docosahexaenoic acid and eicosapentaenoic acid production under good manufacturing practices over 2 decades. In recent years, it has claimed attention for novel biotechnological applications, such as a functional feed additive for animals. Studies have demonstrated that this yeast is s...

The overuse of antibiotics in aquaculture has led to serious concerns on microbial resistance and chemical residues. Novel sources of immunostimulants could help to solve this problem by stimulating the immune system to fight against pathogens. Therefore, this study aims to explore the immunostimulant potential of Cystobacidium benthicum-β-glucans...

The effect of dietary inulin on the intestinal bacterial communities of Penaeus vannamei by 16S metagenomic analysis was assessed. PCR amplified the V3 region of the bacterial 16S rDNA. Sequencing reads were generated using the 2×150 (300 cycles) for the base-read length chemistry of the Illumina MiniSeq platform. The software Shaman and Microbiome...

Resumen La Moringa oleifera Lam es un árbol caducifolio de rápido crecimiento, debido a su plasticidad ecológica, perfil de metabolitos primarios y secundarios, composición mineral, y contenido de aminoácidos, es dentro del género Moringácea la especie más cultivada. Debido a esta es una planta que ha recibido especia atención para combatir la desn...

Mycobacterium avium subsp. paratuberculosis (MAP) is the etiological agent of Paratuberculosis, a contagious, untreatable, and chronic granulomatous enteritis that results in diarrhea, emaciation, and death in farmed ruminants (i.e., cattle, sheep, and goats). In this study, the Ag85B antigen from MAP was expressed in transgenic alfalfa as an attra...

This study determined (1) phytochemical and antioxidant properties of moringa (Moringa oleifera Lam.) seed extract obtained by Naviglio's method; (2) antimicrobial activity; and (3) immunostimulant properties using Longfin yellowtail leukocytes. The results by Fourier Transform Infrared (FTIR) spectroscopy showed that moringa seed extract (MoSE) co...

Some synthetic fungicides have been currently prohibited due to their adverse effects; thus, searching for alternatives to decrease their application is a priority worldwide. An alternative to the application of synthetic fungicides is chitosan -a natural biopolymer- because of its biocompatibility, biodegradability, and bioactivity. Chitosan has b...

This study investigated Lippia palmeri Watt (oregano) phytochemical compounds, their antioxidant capacity, and immunological effects on goat peripheral blood leukocytes (PBL), and on the presence of intermediate polar compounds in goat feces fed dietary oregano. The polar and nonpolar fractions of L. palmeri W. were characterized and phytochemical...

Among the aquaculture activity, which is among the most important in the world, there are factors that impede the expected development and cause losses of millions of dollars. One of these factors is vibriosis, one of the most infectious diseases and it has a great impact on this activity. The objective of this article is to expose the characterist...

Introduction Three decades of evidences have demonstrated that plants are an affordable platform for biopharmaceutical production and delivery. For instance, several plant-made recombinant proteins have been approved for commercialization under good manufacturing practice (GMP). Thus far, plant-based vaccine prototypes have been evaluated at pre- a...

Plant‐mediated nanoparticle synthesis is an eco‐friendly method designed to reduce toxicity. This research reports the effects of gold nanoparticle green synthesis on immune modulation for the first time, using aqueous extract from Turnera diffusa. First, the chemical composition showed that T. diffussa is abundant in compounds, such as oplopanone,...

Introduction Microorganisms can develop into a social organization known as biofilms and these communities can be found in virtually all types of environment on earth. In biofilms, cells grow as multicellular communities held together by a self-produced extracellular matrix. Living within a biofilm allows for the emergence of specific properties fo...

Debaryomyces hansenii BCS004 strain is a non‐pathogenic, polyamine and superoxide dismutase secreting marine yeast. The objective of this study was to investigate the marine yeast DhBCS004 as probiotics and evaluate its antimicrobial properties and dietary immunostimulant effect on gilthead seabream specimens when administered at 106 colony‐forming...

Immunostimulant and protective effects of Yarrowia lipolytica glucans against important pathogens, such as Escherichia coli, have not been investigated in goats and other ruminants. This study aimed to characterize Y. lipolytica N6-glucan (Yl-glucan) and its possible role in immunological signaling pathway activation and immunoprotection against E....

In the Aquaculture industry, which is in charge of sustainably supplying food of marine and freshwater origin (fish, mollusks, crustaceans, among others), there are risks that compromise production and sometimes put producers in very critical circumstances. Sanitary issues, caused by accumulated outbreaks which affected the shrimp farming industry...

Interest in green synthesis methods for nanoparticle production with potential use in biology, pharmacy, and medicine is a forefront field in research. Among the routes described in the literature, the use of plant extracts as reducing and capping agents is one of the most promising. Turnera diffusa commonly called Damiana is a wild shrub tradition...

Immunoprophylactic alternatives are needed to fight against acute hepatopancreatic necrosis disease (AHPND). This study addressed the effects of different routes of Bacillus administration (via diet or culture water) on growth performance, survival, and immune parameters of Litopenaeus vannamei challenged with Vibrio parahaemolyticus-AHPND. Bacillu...

Background: The Moringa genus is made up of thirteen species and is characterized by its rapid growth. Studies relating to gender in association with plant growth promoting bacteria (PGPB) are limited. Objective: To evaluate the growth, development, and quality of Moringa oleifera Lam seedlings inoculated with plant growth promoting bacteria under...