Carla Pinheiro

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• PhD
• Assistant Professor at Universidade NOVA de Lisboa

Cork, extracted sustainably from cork oak ( Quercus suber ), plays a pivotal role in various ecological, social, and economic aspects of Mediterranean regions. Influenced by genetic and environmental factors, the genetic control of cork production remains poorly understood. This study delves into the involvement of dirigent proteins (DIR) in cork l...

The Mediterranean diet (MD), known for its global popularity, emphasizes fresh, high-quality plant-based foods. It is associated with a reduced risk of several noncommunicable diseases (NCDs). This diet also promotes sustainability by encouraging the consumption of seasonal produce and reducing food waste. A key feature of the Mediterranean diet is...

Soups are one of the most consumed meals around the world. Due to the vegetables used in its preparation, they are recognised as one of the healthiest meals. The consumption of soups provides several health benefits due to the nutritional and nutraceutical properties present in their components. Coincidently, or not, consumption of soup is recommen...

Summary: A diet rich in plant-based has been associated with multiple health benefits, including a reduction in the risk of developing non-communicable diseases caused by unhealthy habits. Soups, rich in bioactive compounds, are common food in the Mediterranean diet, being associated with an overall mortality reduction and lower risk of developing...

Cork oak (Quercus suber) covers 22% of the Portuguese forest area. This Mediterranean agroforestry system generates diverse goods and services, Portugal being the world’s largest cork producer. Cork, the outer layer that covers the trunk of the cork oak tree, is well known as the raw material of bottle stoppers. However, as it is rich in phenolic c...

To explore the connection between chloroplast and coffee resistance factors, designated as SH1 to SH9, whole genomic DNA of 42 coffee genotypes was sequenced, and entire chloroplast genomes were de novo assembled. The chloroplast phylogenetic haplotype network clustered individuals per species instead of SH factors. However, for the first time, it...

Plants have evolved sophisticated mechanisms to coordinate carbon metabolism during growth and development both under optimal and stress conditions. In coffee-rust biotrophic interactions, plants try to limit pathogen access to nutrients (e.g., sugars and sugar derivatives) and trigger immune responses, while Hemileia vastatrix (Hv) attempts to cir...

In Mediterranean traditional agricultural systems, chickpea (Cicer arietinum L.), the third most important pulse crop in the world, is a spring crop and yield is extremely affected by soil water availability during the reproductive phase. As a consequence, there is a need to develop chickpea germplasm with resistance to drought and other abiotic st...

Chickpea (Cicer arietinum) is a major player in the FAO Zero Hunger program "toolkit". It is the second most cultivated food legume, a source of sustainable protein (and other nutrients) and contributes to improved soil health and lower fertilization input. Grain yield, seed protein content and nutritional quality were found to be largely affected...

To explore the connection between chloroplast and coffee resistance factors, designated as SH1 to SH9, the whole genome of 42 coffee genotypes was sequenced and de novo assembled. The chloroplast phylogenetic haplotype network clustered individuals per species instead of SH factors. However, for the first time, it allowed the molecular validation o...

Cork oak (Quercus suber) covers 22% of the Portuguese forest area. This Mediterranean agroforestry system generates diverse goods and services, Portugal being the world’s largest cork producer. Cork, the outer layer that covers the trunk of the cork oak tree, is well known as the raw material of bottle stoppers. However, as it is rich in phenolic c...

Cork oak (Quercus suber) covers 22% of the Portuguese forest area. This Mediterranean agroforestry system generates diverse goods and services, Portugal being the world’s largest cork producer. Cork, the outer layer that covers the trunk of the cork oak tree, is well known as the raw material of bottle stoppers. However, as it is rich in phenolic c...

Citation: Pinheiro, C.; Emiliani, G.; Marino, G.; Fortunato, A.S.; Haworth, M.; De Carlo, A.; Chaves, M.M.; Loreto, F.; Centritto, M. Metabolic Background, Not Photosynthetic Physiology, Determines Drought and Drought Recovery Responses in C3 and C2 Moricandias. Int. J. Mol. Sci. 2023, 24, 4094. https://doi. Abstract: Distinct photosynthetic physio...

Chickpea (Cicer arietinum L.) is an important grain legume crop in the world. In the Mediterranean regions, and under rainfed conditions, grain production depends on the climatic variability over years. Particularly important are rainfall amount and distribution, as well as high temperatures during the reproductive phase. Problematic 1 There is a l...

Chickpea (Cicer arietinum L.) is an important grain legume crop in the world. In the Mediterranean regions, and under rainfed conditions, grain production depends on the climatic variability over years. Particularly important are rainfall amount and distribution, as well as high temperatures during the reproductive phase. Problematic 1 There is a l...

Coffee leaf rust (Hemileia vastatrix) is the most important disease of Coffea arabica. This project aims to identify coffee’s primary metabolic components essential for growth and development that are simultaneously involved in plant defense responses. We studied the interaction of two contrasting coffee genotypes [C. arabica S4 Agaro and Kawisari...

The increased warming and precipitation decline in the Mediterranean region makes it a climate change hotspot (Cos et al., 2022). The foreseen temperature increase in this region (0.03°C rise per year if the current trend is maintained), surpasses the global average increase (0.02°C rise per year; IPCC, 2019; UNEP, 2020). Additionally, the water sc...

Epidemics of coffee leaf rust (CLR) leads to great yield losses and huge depreciation of coffee marketing values, if no control measures are applied. Societal expectations of a more sustainable coffee production are increasingly imposing the replacement of fungicide treatments by alternative solutions. A protection strategy is to take advantage of...

The Mediterranean climate is characterized by hot dry summers and frequent droughts. Mediterranean crops are frequently subjected to high evapotranspiration demands, soil water deficits, high temperatures, and photo-oxidative stress. These conditions will become more severe due to global warming which poses major challenges to the sustainability of...

Cork is a renewable, non-wood high valued forest product, with relevant ecological and economic impact in the Mediterranean-type ecosystems. Currently, cork is ranked according to its commercial quality. The most valuable planks are chosen for cork stoppers production. Cork planks with adequate thickness and porosity are classified as stoppable qua...

Main conclusions Arabidopsis and Eutrema show similar stomatal sensitivity to drying soil. In Arabidopsis, larger metabolic adjustments than in Eutrema occurred, with considerable differences in the phytohormonal responses of the two species. Although plants respond to soil drying via a series of concurrent physiological and molecular events, droug...

Vascularization in complex human tissues remains a challenge in tissue engineering. Recent research on whole organ decellularization has showcased advantages regarding biocompatibility and structural integrity on the resulting extracellular matrix. In this work, we explored the possibilities of plant decellularization for human organ regeneration.

Sugar beet (Beta vulgaris L.) high yields have been achieved through irrigation and this crop is described as coping with mild salinity (40–120 mM NaCl). However, during seed germination, sugar beet is salinity sensitive and soil salinity should not exceed 3 dS/m. Wild beets, ancestors of sugar beet, are naturally able to germinate and grow in sali...

Apoiar e promover a fenotipagem de plantas em larga escala de coleções de germoplasma, com recurso a tecnologias cada vez mais eficientes e fáceis de operar. Em 2014 foi iniciada uma rede trans-europeia COST focada nesta temática, que reúne investigado-res e melhoradores de 28 países europeus. O melhoramento tradicional Desde sempre, agricultores e...

Main conclusion: The phenylpropanoid pathway impacts the cork quality development. In cork of bad quality, the flavonoid route is favored, whereas in good quality, cork lignin and suberin production prevails. Cork oaks develop a thick cork tissue as a protective shield that results of the continuous activity of a secondary meristem, the cork cambi...

Fruits may be exposed to several unfavourable mechanical, climatic and biological factors along ripening. Harvest and storage also challenge fruit integrity before fruits reach the consumer. In order to preserve its properties/characteristics it is essential that the structural and chemical integrity of the cuticle is maintained throughout fruit de...

Proteins and peptides able to resist gastrointestinal digestion and reach the intestinal mucosa have the potential to influence human health. Chickpea (Cicer arietinum L.) seed proteins are able to resist cooking (86.9% total protein) and/or in vitro simulated human digestion (15.9% total protein resists soaking, cooking and digestion with pepsin a...

Drought is one of the major constraints limiting crop production worldwide including grapevine. Investigations of drought tolerance genotypes by genetic engineering are an important goal in Vitis breeding program. Three dehydration-responsive RD22 genes (VviRD22) were identified in Vitis vinifera L. Here, we aim to evaluate the constitutive express...

The impact of water deficit on berry quality has been extensively investigated during the last decades. Nonetheless, there is a scarcity of knowledge on the performance of varieties exposed to a combination of high temperatures/water stress during the growing season and under vineyard conditions. The objective of this research was to investigate th...

The establishment of stress resilient sugar beets (Beta vulgaris spp. vulgaris) is an important breeding goal since this cash crop is susceptible to drought and salinity. The genetic diversity in cultivated sugar beets is low and the beet wild relatives are useful genetic resources for tolerance traits. Three wild beet populations (Beta vulgaris sp...

The extracellular space (ECS or apoplast) is the plant cell compartment external to the plasma membrane, which includes the cell walls, the intercellular space and the apoplastic fluid (APF). The present review is focused on APF proteomics papers and intends to draw information on the metabolic processes occurring in the ECS under abiotic and bioti...

Stomatal regulation of leaf gas exchange with the atmosphere is a key process in plant adaptation to the environment, particularly in regions with high atmospheric evaporative demand. Development of stomata, integrating internal signaling and environmental cues sets the limit for maximum diffusive capacity of stomata, through size and density and i...

The primary objective of crop breeding is to improve yield and/or harvest quality while minimizing inputs. Global climate change and the increase in world population are significant challenges for agriculture and call for further improvements to crops and the development of new tools for research. Significant progress has been made in the molecular...

A proteomic analysis of the apoplastic fluid (APF) of coffee leaves was conducted to investigate the cellular processes associated with incompatible (resistant) and compatible (susceptible) Coffea arabica-Hemileia vastatrix interactions, during the 24–96 hai period. The APF proteins were extracted by leaf vacuum infiltration and protein profiles we...

A proteomic analysis of the apoplastic fluid (APF) of coffee leaves was conducted to investigate the cellular processes associated with incompatible (resistant) and compatible (susceptible) Coffea arabica – Hemileia vastatrix interactions, during the 24– 96 hai period. The APF proteins were extracted by leaf vacuum infiltration and protein profiles...

O presente documento foi elaborado pela FILCORK - Associação interprofissional da fileira da cortiça no âmbito da Operação “Programa de Valorização da Cortiça no Alentejo” (ALENT-07-0827-FEDER-001499), uma iniciativa QREN apoiada pelo INALENTEJO.

The coffee leaf rust (Hemileia vastatrix) disease causes serious losses in coffee production and quality, with a huge depreciation of marketing values. The reduction of availability of effective approved fungicides, due to health and environmental concerns, makes it necessary to intensify research for the development of novel, effective and sustain...

The coffee leaf rust (Hemileia vastatrix) disease causes serious losses in coffee production and quality, with a huge depreciation of marketing values. The reduction of availability of effective approved fungicides, due to health and environmental concerns, makes it necessary to intensify research for the development of novel, effective and sustain...

PLANT BIOLOGY EUROPE FESPB/EPSO 2014 Congress Poster Presentation 2014, Dublin, Ireland

Mediterranean woody species are vulnerable to multiple stresses that negatively affect plant survival and productivity. Drought and heat are increasing threats to the agricultural and forestry systems making it urgently necessary to decipher the mechanisms at the basis of plant adaptation and survival. Proteomics allows characterisation of a large...

Cork is one of the most valuable non-wood forest products and plays an important role in Mediterranean economies. The production of high-quality cork is dependent on both genome and environment, posing constraints on the industry because an ever-growing amount of bad-quality cork (BQC) development has been observed. In order to identify genes respo...

Allergies are nearly always triggered by protein molecules and the majority of individuals with documented immunologic reactions to foods exhibit IgE hypersensitivity reactions. In this study we aimed to understand if natural differences, at proteomic level, between maize populations, may induce different IgE binding proteins profiles among maize-a...

The seed proteome of two traditional maize inbred lines (pb269 and pb369) contrasting in grain hardness and in preferable use for bread-making was evaluated. The pb269 seeds, of flint type (i.e. hard endosperm) are preferably used by manufacturers while pb369 (dent, soft endosperm) is rejected. The hypothesis that the content and relative amounts o...

Coffee leaf rust, caused by the fungus Hemileia vastatrix Berk & Br., is the most widespread disease of Coffea arabica L. cultivars. Coffee – rust interactions are governed by the gene-for-gene relationship, being the resistance conditioned at least by nine major dominant genes (S H 1-S H 9) singly or associated. A cytologic and proteomic approach...

In the present study, field grown Aragonez (Syn. Tempranillo) grapevines (Vitis vinifera L.) were subjected to three irrigation regimes (conventional sustained deficit irrigation (DI), regulated deficit irrigation (RDI) and non-irrigated (NI)) during two successive seasons (2007–2008). An integrative study was performed in grape berry skin tissues...

SUMMARY Coffee leaf rust, caused by the fungus Hemileia vastatrix Berk & Br., is the most widespread disease of Coffea arabica L. cultivars. Coffee – rust interactions are governed by the gene-for-gene relationship, being the resistance conditioned at least by nine major dominant genes (SH1-SH9) singly or associated. A cytologic and proteomic appro...

The early (2-4 d) effects of slowly imposed soil water deficit on Lupinus albus photosynthetic performance, carbon metabolism, and hormonal balance in different organs (leaf blade, stem stele, stem cortex, and root) were evaluated on 23-d-old plants (growth chamber assay). Our work shows that several metabolic adjustments occurred prior to alterati...

We analysed the changes in the metabolites of Lupinus albus organs (leaf-blades, petioles, apexes, hypocotyls and roots) as a consequence of B deficiency. The deficiency did not affect malate concentration and induced only minor changes in the sugar content, suggesting that the carbohydrate metabolism is little affected by the deficiency. Contraril...

Background • Chickpea (Cicer arietinum L.) →3 rd most important grain legume crop in the world →1 st in the Mediterranean basin and south Asia • Drought stress (during the reproductive stage) →seed yield loss →decreased nutritional value 1,2 Goal →To evaluate seed quality (fat, starch, protein content and protein digestibility) in two elite cultiva...

Photosynthesis is one of the key processes to be affected by water deficits, via decreased CO2 diffusion to the chloroplast and metabolic constraints. The relative impact of those limitations varies with the intensity of the stress, the occurrence (or not) of superimposed stresses, and the species we are dealing with. Total plant carbon uptake is f...

The characterization of the wild beet relatives, as well as their use for the selection of useful traits, is limited by difficulties associated with the germination of these plants. Currently available germination protocols deliver low percentage, unsynchronised and lengthy germination. We tested several procedures likely to weaken the woody/corky...

A collection of 155 accessions of ancient Portuguese common beans (Phaseolus vulgaris L) was evaluated in relation to the content of 8 minerals (Zn, Cu, Fe, Mn, Ca, Mg, P and K) important for human nutrition. A high degree of variability for P. Fe, Zn, Cu, Mn and Ca was observed in the collection. Total correlation matrix analysis revealed the exis...