Camillo Almici

Camillo Almici
ASST Spedali Civili di Brescia · Trasfusion Medicine

laurea in Medicina

About

150
Publications
6,801
Reads
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2,382
Citations
Introduction
The primary task of our laboratory is to support the BMT program at the ASST Spedali Civili in Brescia. We are in charge of the manipulation and cryopreservation of hematopoietic progenitor cell units from patients and from HLA-matched familiar or unrelated donors in the allogeneic setting. Besides our routine clinical duties, our research activity concerns mesenchymal stromal cells expansion from different sources, and more recently, our efforts have focused on the role of circulating endothelial cells (CEC) in allo-transplantation. By using different CEC identification and counting procedures, we have been involved in studying the possible correlations of CEC count changes with the endothelial complications of allogeneic hematopoietic stem cells transplantation.
Additional affiliations
January 2005 - present
A.O. Spedali Civili di Brescia
Position
  • Medical Director, Lab for Manipulation and Cryopreservation of Stem Cells
January 1998 - December 2004
Spedali Civili di Brescia
Position
  • Medical Staff
September 1992 - February 1993
University of Minnesota Twin Cities
Position
  • Instructor
Education
September 2011 - November 2011
Scuola di Direzione in Sanità – Regione Lombardia
Field of study
  • Formazione manageriale in sanità
September 2008 - September 2008
Agenzia Italiana del Farmaco – Area Produzione e Controllo, Ufficio Autorizzazione Officine
Field of study
  • Decreto Legislativo 24 aprile 2006, n.219
September 2002 - March 2003
Scuola di Direzione in Sanità – Regione Lombardia
Field of study
  • Formazione manageriale in sanità

Publications

Publications (150)
Article
Full-text available
Circulating endothelial cells (CEC) and their progenitors (EPC) are restricted subpopulations of peripheral blood (PB), cord blood (CB), and bone marrow (BM) cells, involved in the endothelial homeostasis maintenance. Both CEC and EPC are thought to represent potential biomarkers in several clinical conditions involving endothelial turnover/remodel...
Article
Full-text available
Background. Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is burdened by life-threatening complications, with graft-versus-host disease (GvHD) being the major cause of morbility and mortality. Recently, clinical and physiopathologic evidences showed that vascular endothelium can be a target of GvHD in the early phase and circulatin...
Article
Full-text available
Introduction We investigated the frequency of detection and the prognostic and predictive significance of circulating tumor cells (CTCs) in patients with recurrent/metastatic (R/M) head and neck carcinoma (HNC) before starting systemic therapy. Patients and methods Using the CellSearch technology, CTCs were assessed prospectively in peripheral blo...
Article
Full-text available
High dose chemotherapy with autologous peripheral blood stem cell rescue has been reported as feasible and effective in HIV-associated lymphoma. Although a sufficient number of stem cell seems achievable in most patients, there are cases of stem cell harvest failure. The aim of this study is to describe the mobilization policies used in HIV-associa...
Article
Full-text available
A role of endothelial cells (ECs) in Primary Myelofibrosis (PMF) was supposed since JAK2 mutation was found in endothelial precursor cells (EPCs) and in ECs captured by laser microdissection. By Cell Search method, the circulating endothelial cells (CECs) from 14 PMF patients and 5 healthy controls have been isolated and compared by NGS with CD34+H...
Article
Full-text available
Gelatin–dextran hydrogel scaffolds (G-PEG-Dx) were evaluated for their ability to activate the bone marrow human mesenchymal stromal cells (BM-hMSCs) towards mineralization. G-PEG-Dx1 and G-PEG-Dx2, with identical composition but different architecture, were seeded with BM-hMSCs in presence of fetal bovine serum or human platelet lysate (hPL) with...
Article
Selection of feasible hybrid-hydrogels for best chondrogenic differentiation of human mesenchymal stromal cells (hMSCs) represent an important challenge in cartilage regeneration. In this study, three-dimensional hybrid hydrogels obtained by chemical crosslinking of poly (ethylene glycol) diglycidyl ether (PEGDGE), gelatin (G) without or with chito...
Article
Full-text available
Human platelet lysate (hPL) is considered a valid substitute to fetal bovine serum (FBS) in the expansion of mesenchymal stromal cells (MSC), and it is commonly produced starting from intermediate side products of whole blood donations. Through freeze–thaw cycles, hPL is highly enriched in chemokines, growth factors, and adhesion and immunologic mo...
Article
Full-text available
Regenerative medicine aims to restore damaged tissues and mainly takes advantage of human mesenchymal stromal cells (hMSCs), either alone or combined with three-dimensional scaffolds. The scaffold is generally considered a support, and its contribution to hMSC proliferation and differentiation is unknown or poorly investigated. The aim of this stud...
Article
Full-text available
One of the main hurdles to improving scaffolds for regenerative medicine is the development of non-invasive methods to monitor cell proliferation within three-dimensional environments. Recently, an electrical impedance-based approach has been identified as promising for three-dimensional proliferation assays. A low-cost impedance-based solution, ea...
Article
Background. We recently reported that Circulating Endothelial Cell (CEC) count changes represent a promising marker to monitor endothelial damage in patients undergoing allogeneic hematopoietic stem cell transplant (allo-HSCT), potentially becoming a valuable tool in the diagnostic definition of GVHD. Besides confirming an increase of CEC counts at...
Article
Full-text available
Cartilage tissue engineering remains problematic since no systems are able to induce signals that contribute to native cartilage structure formation. Therefore, we tested the potentiality of gelatin‐polyethylene glycol scaffolds containing three different concentrations of chitosan (CH) (0%, 8% and 16%) on chondrogenic differentiation of human plat...
Data
Supplementary_material_with_revision – Supplemental material for 3D gelatin-chitosan hybrid hydrogels combined with human platelet lysate highly support human mesenchymal stem cell proliferation and osteogenic differentiation
Data
Supplementary_Table_1_rev – Supplemental material for 3D gelatin-chitosan hybrid hydrogels combined with human platelet lysate highly support human mesenchymal stem cell proliferation and osteogenic differentiation
Article
Full-text available
Bone marrow and adipose tissue human mesenchymal stem cells were seeded in highly performing 3D gelatin–chitosan hybrid hydrogels of varying chitosan content in the presence of human platelet lysate and evaluated for their proliferation and osteogenic differentiation. Both bone marrow and adipose tissue human mesenchymal stem cells in gelatin–chito...
Article
Full-text available
Physio-pathologic interrelationships between endothelial layer and graft-versus-host disease (GVHD) have been described leading to assess the entity “endothelial GVHD” as the early step for clinical manifestations of acute GVHD. The availability of the CellSearch system has allowed us to monitor Circulating Endothelial Cells (CEC) changes in alloge...
Article
Background: The heterogeneity of metastatic breast cancer (MBC) necessitates novel biomarkers allowing stratification of patients for treatment selection and drug development. We propose to use the prognostic utility of circulating tumor cells (CTCs) for stratification of patients with stage IV disease. Methods: In a retrospective, pooled analys...
Article
Introduction: Autologous stem cell transplantation (ASCT) is considered safe and effective in patients with HIV-associated lymphoma (HIV-Ly). The reported studies usually analyze togheter different histological subtypes, including classical Hodgkin lymphoma (HL) and a variety of aggressive non Hodgkin lymphoma (NHL), and different indications for A...
Article
Melanoma is an immunogenic neoplasm infiltrated by T cells, although these adaptive T cells usually fail to eradicate the tumor. Plasmacytoid dendritic cells (PDCs) are potent regulators of the adaptive immune response and can eliminate melanoma cells via TLR-mediated effector functions. The PDC compartment is maintained by progressively restricted...
Article
Full-text available
Circulating endothelial cells (CEC) represent a restricted peripheral blood (PB) cell subpopulation with high potential diagnostic value in many endothelium-involving diseases. However, whereas the interest in CEC studies has grown, the standardization level of their detection has not. Here, we undertook the task to align CEC phenotypes and counts,...
Article
Full-text available
HIV-infected patients with high risk non-Hodgkin lymphoma (NHL), identified by the International Prognostic Index (IPI),1, 2 have a sub-optimal outcome if treated with standard chemotherapy. In the combination antiretroviral therapy (cART) era, in a randomised trial, the CR rate with CHOP+Rituximab was 46.6% in patients with aa-IPI 2–3. Moreover, t...
Article
Full-text available
The physio-pathologic interrelationships between endothelium and GvHD have been better elucidated and have led to definition of the entity ‘endothelial GvHD’ as an essential early phase prior to the clinical presentation of acute GvHD. Using the CellSearch system, we analyzed circulating endothelial cells (CEC) in 90 allogeneic hematopoietic stem c...
Article
Full-text available
A human bone marrow-derived mesenchymal stromal cell (MSCs) and cord blood-derived CD34+ stem cell co-culture system was set up in order to evaluate the proliferative and differentiative effects induced by MSCs on CD34+ stem cells, and the reciprocal influences on gene expression profiles. After 10 days of co-culture, non-adherent (SN-fraction) and...
Data
IPA analysis of MSCs after co-culturing with CB-CD34+ cells. The plot is representative of cardiovascular system development and function, cellular development and haematological system development and function networks. (TIFF)
Data
Gene Set Enrichment Analysis (GSEA) of MSCs-alone vs. co-cultured MSCs. The table reports Hellmark Genes, Oncogenic-linked Genes, Immune-linked Genes and Pathways enriched after co-culturing. Each category is reported in a different sheet, where the Gene Set Name, the number of Genes in Gene Set (K), the Description, the number of Genes in Overlap...
Data
Flow cytometry detection of CB-CD34+ cells after immunomagnetic enrichment. (TIFF)
Data
IPA analysis of CB-CD34+ cells co-cultured with MSCs. The network is representative of cellular growth and proliferation, haematological system development and function and haematopoietic pathways. (TIFF)
Data
IPA analysis of MSCs after co-culture with CB-CD34+ cells. The plot is representative of cell-to-cell signaling and interaction, cellular movement, and immune cell trafficking networks. (TIFF)
Data
Characterisation of BM-derived MSC differentiation potential. Osteogenic, chondrogenic and adipogenic differentiation was assessed after culture in proper media and specific staining. (TIFF)
Data
Schematic representation of the cellular growth and proliferation network as identified by IPA pathway analysis and specifically related to hematopoiesis. Key regulator genes are highlighted by blue circles. (TIFF)
Data
Gene Set Enrichment Analysis (GSEA) of CB-alone vs. total SN+AD. The table reports Hellmark Genes, Oncogenic-linked Genes, Immune-linked Genes and Pathways enriched after co-culture. Each category is reported in a different sheet, where the Gene Set Name, the number of Genes in Gene Set (K), the Description, the number of Genes in Overlap (k), the...
Data
Flow cytometry detection of CB-CD34+ cells. HSCs were considered to be CD45+ dim CD34+. (TIFF)
Data
IPA analysis on CB-CD34+ cells co-cultured with MSCs. The network is representative of cell cycle, cell death and survival pathways. (TIFF)
Data
IPA analysis of CB-CD34+ cells co-cultured with MSCs. The network is representative of cell-to-cell signaling and interaction pathways. (TIFF)
Data
Ex vivo expansion of CB-CD34+ cells with MSCs. The table summarise the results about the assessment of the ex vivo expansion of CB-CD34+ cells grown in presence of MSCs. Briefly, 5×105 CB-CD34+ cells were cultured alone or in presence of a layer of MSCs for 10 days; the fold increase in total cell number was calculated from the original CD34+ cells...
Data
Ingenuity Pathway Analysis (IPA) of CB-alone vs. total SN+AD. The table reports the networks in which the differentially expressed genes (in bold characters) are involved. Columns list the Molecules in Network, the Score, the Focus-molecules and the direct related Top Diseases and Functions Molecules. (DOCX)
Data
Ingenuity Pathway Analysis (IPA) of MSCs-alone vs. co-cultured MSCs. The table reports the networks in which the differential expressed genes (in bold characters) are involved. Columns list the Molecules in Network, the Score, the Focus-molecules and the direct related Top Diseases and Functions Molecules. (DOCX)
Article
Full-text available
Background: Circulating tumor cells have been described in prostate cancer patients at diagnosis and in the metastatic phase but little is known on their role at biochemical PSA recurrence. Patients and methods: Patients radically cured with either prostatectomy or radiotherapy were sequentially included at PSA recurrence. The presence of CTCs w...
Conference Paper
Introduction: Mesenchymal stromal cells (MSCs) play a main role in the organization of the hematopoietic niche (BM-niche) by promoting cell and matrix interactions and by secreting different growth factors (i.e. SDF-1, SCF, TPO, IL-6, IL-11). Intra-Bone Stem Cell Transplantation (IB-HSCT), based on the direct inoculation of umbilical cord blood CD3...
Article
BACKGROUND: Based on therapeutic equivalence extrapolated from originator, the use of biosimilar Granulocyte-Colony Stimulating Factor (G-CSF) in the context of stem cell harvest was approved by the European Medicines Agency (EMA), however “ad hoc” studies are limited. AIMS: To assess the efficacy and safety profile of biosimilar compared with orig...
Article
Background. Outcome of HIV-associated NHL (HIV-NHL) with adverse prognostic features is not satisfactory with standard therapy. High dose therapy (HDT) and autologous stem cell transplantation (ASCT) has proven safe and active in HIV-NHL in salvage setting. Aims. To define the role of HDT and ASCT in the upfront treatment of HIV-NHL at high risk, i...
Article
Autologous stem cell transplantation (ASCT) is a potentially curative treatment of lymphoma, but peripheral blood stem cell (PBSC) mobilization fails in some patients. PBSC mobilizing agents have recently been proved to improve the PBSC yield after a prior mobilization failure. Predictive parameters of mobilization failure allowing for a preemptive...
Conference Paper
Introduction: Allo-HSCT can be burdened by life-threatening complications, being GVHD the major cause of morbility and mortality. Clinical and physio-pathological evidences showed that vascular endothelium could be a target of GVHD in very early phase; therefore markers of endothelial damage are warranted as valuable support in GVHD diagnosis. We c...
Article
Full-text available
We aimed to assess the clinical validity of circulating tumour cell (CTC) quantification for prognostication of patients with metastatic breast cancer by undertaking a pooled analysis of individual patient data. We contacted 51 European centres and asked them to provide reported and unreported anonymised data for individual patients with metastatic...
Article
Background: Clinical validity of CTCs (CellSearch®) in metastatic breast cancer (MBC) patients has previously been assessed in studies with limited statistical power. We aimed to pool all European studies to obtain high-level evidence on the prognostic value of CTCs, to investigate their effects across different clinico-pathological characteristics...
Article
Allo-HSCT can be burdened by life-threatening complications, being GvHD the major cause of morbility and mortality. Clinical and physio-pathological evidences showed that vascular endothelium could be a target of GvHD in very early phase; therefore markers of endothelial damage are warranted as valuable support in GvHD diagnosis. We conducted a stu...
Article
Full-text available
The stromal progenitors of mesodermal cells, mesenchymal stromal cells (MSCs), are a heterogeneous population of plastic adherent fibroblast-like cells with extensive proliferative capacity and differentiation potential. Human MSCs have now been isolated from various tissues including bone marrow, muscle, skin, and adipose tissue, the latter being...
Article
3124 Background Outcome of aggressive HIV-associated NHL (HIV-NHL) with adverse prognostic features is not satisfactory with standard therapy. High dose therapy (HDT) and autologous stem cell transplantation (ASCT) has been demonstrated safe and active in HIV-NHL in the salvage setting. Aims To define the role of HDT and ASCT in the upfront treat...
Conference Paper
Full-text available
Background We evaluated the safety and efficacy of pmab, a fully human monoclonal antibody against the epidermal growth factor receptor, by comparing PRT with CRT in pts with LASCCHN.
Article
The mechanism of dissemination of locally advanced head and neck cancer (LAHNC) is far to be resolved. Circulating Tumour Cells (CTC) have been identified as a prognostic factor in metastatic breast and prostate cancer. This prospective multi-centric analysis studied the possible role of CTC identification in LAHNC. CTC were searched in 73 patients...