Camila Lima

Camila Lima
Laval University | ULAVAL · Department of Animal Sciences

PhD in Biotechnology

About

45
Publications
2,797
Reads
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226
Citations
Citations since 2017
36 Research Items
222 Citations
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2017201820192020202120222023010203040506070
2017201820192020202120222023010203040506070
2017201820192020202120222023010203040506070

Publications

Publications (45)
Preprint
Full-text available
Pyruvate, the end-product of glycolysis in aerobic conditions, is produced by cumulus cells, and is converted in Acetyl-CoA into the mitochondria of both cumulus cells (CCs) and oocytes as a master fuel input for the tricarboxylic acid cycle (TCA). The citrate generated in the TCA cycle can be directed to the cytoplasm and converted back to acetyl-...
Article
Metabolism and epigenetics, which reciprocally regulate each other in different cell types, are fundamental aspects of cellular adaptation to the environment. Evidence in cancer and stem cells has shown that the metabolic status modifies the epigenome while epigenetic mechanisms regulate the expression of genes involved in metabolic processes, ther...
Article
In the present study, we investigated the spent culture media of in vitro produced (IVP) bovine embryos which did (group Pregnant) or did not (group Non‐pregnant) establish pregnancy after transfer. For that purpose, IVP embryos on D5 were transferred to individual droplets for the last 48 hours of culture. Embryos at the blastocyst stage were then...
Article
Porcine spermatogonia stem cells (pSSCs) are the only type of somatic stem cell that can pass genetic information to the successive generations. Little is known about pSSCs vitality in vitro, and due to their increasing importance in stem cell research, here, we optimized a protocol to culture pSSCs and explored their potential fate in vitro. Utili...
Article
Metabolic and molecular profiles were reported as different for bovine embryos with distinct kinetics during the first cleavages. In this study, we used this same developmental model (fast vs slow) to determine if the relationship between metabolism and developmental kinetics affects the levels of acetylation or tri-methylation at histone H3 lysine...
Article
Reproducing the environment to which the embryo is naturally exposed may be an alternative to improve viability of embryos produced in vitro. In the first part of this work, we describe a novel culture media, namely Embryonic Culture Supplementation (ECS100). The composition of this media was based on the contents of carbohydrates and amino acids f...
Article
The quantity of lipids available for the initial development processes of in vitro-produced (IVP) embryos can reflect directly on embryonic quality and pregnancy rates. Therefore, the objective of this study was to investigate the lipid profile of IVP embryos from Bos indicus females with different antral follicle counts (AFCs). For this purpose, o...
Article
The present study aimed to identify biomarkers to assess the quality of in vitro produced (IVP) bovine embryos in the culture media. IVP embryos on Day (D) 5 of development were transferred to individual drops, where they were maintained for the last 48 h of culture. Thereafter, the medium was collected and the embryos were transferred to the recip...
Poster
DNA methylation/demethylation is one of several epigenetic mechanisms by which metabolism regulates gene expression. More specifically, α-ketoglutarate (αKG) and succinate (Suc) are tricarboxylic acid cycle metabolites that may decrease and increase, respectively, the activity of DNA demethylases. Because pre-implantation embryos undergo reprogramm...
Poster
During invitro maturation (IVM), bovine oocytes undergo important metabolic, epigenetic, and transcriptional changes for the acquisition of developmental competence. Particularly, metabolic changes that alter the availability of cytoplasmic acetyl-CoA, the main substrate for histones acetylation, may alter the epigenetic profile of the oocyte, with...
Poster
Changes in the dynamics of energy metabolism can affect the sophisticated molecular control of different cell types including embryonic stem cells (Zhang et al. 2018 Cell Metab. 27, 332-338; https://doi.org/10.1016/j.cmet.2018.01.008). In this study, we modulated pathways related to acetyl-CoA generation, the major donor for histone acetylation, an...
Poster
Invitro production (IVP) of embryos is designed to reproduce an environment that resembles the female reproductive tract. However, the system does not perform optimally in terms of quality and embryo production. A major setback lies in the loss of dynamics observed in a static invitro system, which might affect the availability of substrates that r...
Poster
Lipid metabolism provides a potent source of energy and has an important role in the acquisition of oocyte competence. However, there are conflicting reports about how lipid exposure during invitro maturation (IVM) impacts the gamete and further embryo development. In this study, we performed IVM of oocytes in the presence of lipid-rich culture med...
Poster
Bovine spermatozoa are specialised cells that require high ATP production for flagellar movement and other physiological events necessary for fertilization. Glycolysis and oxidative phosphorylation (OXPHOS) are the most studied energy pathways in sperm cells and involve metabolites such as glucose, pyruvate, and lactate. Although glycolysis has bee...
Article
Full-text available
Previous studies have discussed the importance of an optimal range of metabolic activity during preimplantation development. To avoid factors than can trigger an undesirable trajectory, it is important to learn how nutrients and metabolites interact to help launching the correct developmental program of the embryo, and how much the in vitro culture...
Article
Full-text available
In many cell types, epigenetic changes are partially regulated by the availability of metabolites involved in the activity of chromatin-modifying enzymes. Even so, the association between metabolism and the typical epigenetic reprogramming that occurs during preimplantation embryo development remains poorly understood. In this work, we explore the...
Article
Full-text available
Understanding preimplantation embryonic development is crucial for the improvement of assisted reproductive technologies and animal production. To achieve this goal, it is important to consider that gametes and embryos are highly susceptible to environmental changes. Beyond the metabolic adaptation, the dynamic status imposed during follicular grow...
Article
Global mitochondrial DNA (mtDNA) methylation has been recently described in bovine and showed particular signatures in both gametes and embryos. Here, we investigated the distribution of mtDNA methylation through strand‐specific mapping of methylation sites to gain perspective on how epigenetic mechanisms can be involved in mitochondrial function....
Poster
Full-text available
Intermediates of the energy metabolism (as acetyl Co-A) can donate their acetyl group to introduce acetylation in histones, establishing a relationship between metabolism and epigenetic control in somatic and embryonic stem cells. Embryos with different kinetics during the first cleavages also have alterations in epigenetic profile as well as in me...
Article
The effectiveness of the use of natriuretic peptide C (NPPC) in the blocking of meiosis has already been proven in several species. However, there are no reports on the use of NPPC in the activation of metabolic processes in embryos. Whereas modulations of cAMP concentrations alter the lipid metabolism of bovine oocytes, the present study aims to e...
Article
Follicular fluid composition and the transcription pattern of granulosa cells were analysed to better comprehend associations between embryo development and morphokinetics. Bovine follicles were punctured and their respective follicular fluid and granulosa cells were collected. Cumulus–oocyte complexes derived from these follicles were matured and...
Article
Full-text available
Background The timing of the first cell divisions may predict the developmental potential of an embryo, including its ability to establish pregnancy. Besides differences related to metabolism, stress, and survival, embryos with different speeds of development present distinct patterns of gene expression, mainly related to energy and lipid metabolis...
Poster
During in vitro production (IVP), blastocysts can be differentiated based on the kinetics of early cleavage. These groups present distinct patterns of global DNA methylation, an epigenetic characteristic generally responsible for suppression (presence of methylation) or activation (absence of methylation) of genes from different biological pathways...
Article
Full-text available
Estabeleceu-se um protocolo novo e eficaz de cultivo individual de embriões bovinos sem o uso de cocultivo e sem compartilhamento de meio visando à análise do metabolismo individual do embrião. Para isso, embriões foram produzidos in vitro por protocolos convencionais em três diferentes tipos de meio: KSOM, SOFaa e KSOM seguido por SOFaa no dia 2....
Article
Full-text available
High oxygen levels during in vitro culture (IVC) can induce oxidative stress through accumulation of reactive oxygen species (ROS), negatively affecting embryo development. This study evaluated the effect of different O 2 tensions during IVC on bovine blastocyst development and transcriptional status, considering transcription factors that play an...
Data
Supplementary Material - Table I. Taqman assays used for gene expression evaluation by qRT-PCR. Supplementary Material - Table II. Genes with difference between the 5%O2 and 20%O2 groups separated by functional pathways. Supplementary Material - Table III. P value of the genes with relative difference between the 5%O2 and 20%O2 groups (in alphabeti...
Article
This observational study aimed to establishing a relationship between lipid peroxidation and endometriosis in women undergoing controlled ovarian hyperstimulation. A total of 79 women were divided into two groups: (i) controls (tubal or male factor); and (ii) endometriosis (stages III/IV). The endometriosis diagnosis was confirmed by videolaparosco...
Article
The kinetics of in vitro produced (IVP) bovine embryos is related to embryo viability, metabolism and epigenetic patterns. Therefore, we believe that embryos with different speeds of development also respond differently to stress. In the present study, we performed global metabolic analysis (MALDI-TOF Mass Spectrometry) of culture media, characteri...
Article
Full-text available
Introduction Secreted molecules could be correlated with the potential of embryonic development. The development of new technologies, such as mass spectrometry (MS), has enabled analyzes in culture medium to favor the determination of embryos viability in order to improve embryo selection. Objectives To perform a non-invasive characterization of th...
Article
Full-text available
Purpose: Polycystic ovary syndrome (PCOS) is an endocrine-metabolic disorder that leads to lower natural reproductive potential and presents a challenge for assisted reproductive medicine because patients may exhibit immature oocyte retrieval and a higher risk of ovarian hyper stimulation syndrome during in vitro fertilization (IVF) treatment. Thi...
Article
Full-text available
This study proposed lipid fingerprinting of human seminal plasma by mass spectrometry as an analytical method to differentiate biological conditions. For this purpose, we chose infertile men as a model to study specific conditions, namely: high and low seminal plasma lipid peroxidation levels (sub-study 1.1), high and low sperm nuclear DNA fragment...

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