Camila A. Knecht- Doctor of Engineering
- PostDoc Position at National Scientific and Technical Research Council
Camila A. Knecht
- Doctor of Engineering
- PostDoc Position at National Scientific and Technical Research Council
Study of antibiotic resistance in herbicide-resistant environmental bacteria in the wetlands of the Paraná River delta
About
17
Publications
2,228
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Introduction
I am currently working at the Institute of Research in Medical Microbiology and Parasitology (IMPaM) dependent on the National Scientific and Technical Research Council (CONICET) and the University of Buenos Aires (UBA). I am part of a research group focused on understanding the mechanisms of resistance to antibiotics. My topic is the identification of genetic platforms that mobilize antibiotic resistance in environmental strains resistant to herbicides in the wetlands of the Paraná River Delta.
Current institution
Additional affiliations
March 2023 - present
Education
October 2015 - June 2020
Field of study
- Antibiotic resistance in wastewater treated with constructed wetlands
March 2006 - June 2014
Universidad Nacional de Rosario
Field of study
- Biotecnología
Publications
Publications (17)
Determining the prevalence of antimicrobial resistance (AMR) in non-clinical settings is vital for better management of the global AMR crisis. Untreated and even treated wastewaters are important sources that release AMR into the environment. Methodologically, it is difficult to generate a comprehensive in situ profile of antibiotic resistance gene...
Objectives:
The worldwide dissemination of carbapenemase-producing Escherichia coli lineages belonging to high-risk clones poses a challenging public health menace. The aim of this work was to investigate genomic features of a colonizing multidrug resistant strain of KPC-producing E. coli from our institution.
Methods:
WGS was done by Illumina M...
According to the World Health Organization, carbapenem-resistant Enterobacteriaceae (CRE) belong to the highest priority group for the development of new antibiotics. Argentina-WHONET data showed that Gram-negative resistance frequencies to imipenem have been increasing since 2010 mostly in two CRE bacteria: Klebsiella pneumoniae and Enterobacter c...
Objectives
: Enterobacter cloacae complex (ECC) has lately awakened interest due to its increasing resistance to carbapenems codified by several genes all over the globe. Even though there are some sequence types (ST) which represent high-risk clones, there is substantial clonal diversity in the ECC. This work aimed to perform whole-genome sequenci...
The environmental prevalence of antimicrobial resistance (AMR) has come into focus under the One Health concept. Wastewater treatment systems are among the significant sources of AMR in the environment. In such systems, it is uncertain to which extent antimicrobials present at sub-inhibitory concentrations constitute a selective pressure for bacter...
Objectives
Isolation of colistin- and carbapenem-resistant Klebsiella pneumoniae (CCR-Kp) is increasing in hospital settings worldwide, which is related to increased morbidity, mortality and healthcare costs. The aim of this work was to perform whole-genome sequencing (WGS), genomic and phylogenetic analysis, and conjugation assays of an extensivel...
In many countries, emission of insufficiently treated wastewater into water bodies appears to be an important factor in spreading clinically relevant antimicrobial resistant bacteria. In this study, we looked for the presence of Enterobacteriaceae strains with resistance to 3rd generation cephalosporin antibiotics in four urban wetlands in southwes...
There is little information about environmental contamination with antibiotic resistance genes (ARG) in Sub-Saharan Africa, home to about 1 billion people. In this study we measured the abundance of three genes (sul1, sul2, and intI1) used as indicators of environmental contamination with ARGs in the sediments of four urban wetlands in southwestern...
Cold storage is a common procedure for liver preservation in a transplant setting. However, during cold ischemia, the liver suffers molecular alterations that can affect its performance. Also, deleterious mechanisms set forth in the storage phase are exacerbated during reperfusion. This study aimed to identify liver proteins associated with injury...
Wastewater treatment plants are hotspots for the development and
spreading of antibiotic resistant bacteria (ARB) and antibiotic
resistance genes (ARG).
Constructed wetlands (CW) are considered an alternative
wastewater treatment option for removal of ARB and ARG.
Questions
Questions (3)
Hi! I am using DADA2 and phyloseq in R and I get slightly different results if I run the commands more than once with the same sample. I am working with 16S rRNA gene amplicons (Illumina, single end reads). Is there some randomness involved in this pipeline?
Any help would be appreciated! Thank you!
Aeromonas isolation agar should be boiled and not autoclaved in order to be sterilized. The problem is that, when it starts boiling (or even before), the agar starts to form clumps. The agar plates seem to work fine, but it is more difficult to spread the samples on it.
Does someone has any suggestion/idea?
Here the data sheet of the media: http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma-Aldrich/Datasheet/1/17118dat.pdf
