Caio Leal-DutraUniversity of Copenhagen · Section for Ecology and Evolution
Caio Leal-Dutra
Phd
About
21
Publications
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Introduction
Additional affiliations
September 2020 - present
Education
November 2015 - January 2020
March 2013 - March 2015
Publications
Publications (21)
Fungus-farming ants cultivate multiple lineages of fungi for food, but, because fungal cultivar relationships are largely unresolved, the history of fungus-ant coevolution remains poorly known. We designed probes targeting >2000 gene regions to generate a dated evolutionary tree for 475 fungi and combined it with a similarly generated tree for 276...
The naturally selected fungal crop (Leucoagaricus gongylophorus) farmed by leafcutter ants shows striking parallels with artificially selected plant crops domesticated by humans (e.g., polyploidy, engorged nutritional rewards, dependence on cultivation). To date, poorly resolved L. gongylophorus genome assemblies based on short-read sequencing have...
Leafcutter ants are dominant herbivores in the Neotropics and rely on a fungus (Leucoagaricus gongylophorus) to transform freshly gathered leaves into a source of nourishment rather than consuming the vegetation directly. Here we report two virus-like particles that were isolated from L. gongylophorus and observed using transmission electron micros...
Pterulaceae was formally proposed to group six coralloid and dimitic genera: Actiniceps (=Dimorphocystis), Allantula, Deflexula, Parapterulicium, Pterula, and Pterulicium. Recent molecular studies have shown that some of the characters currently used in Pterulaceae do not distinguish the genera. Actiniceps and Parapterulicium have been removed, and...
The genus Parapterulicium was first introduced to accommodate two Brazilian species of coralloid fungi with affinities to Pterulaceae (Agaricales). Despite the coralloid habit and the presence of skeletal hyphae, other features, notably the presence of gloeocystidia, dichophyses and papillate hyphal ends, differentiate this genus from Pterulaceae s...
The naturally selected fungal crop ( Leucoagaricus gongylophorus ) farmed by leafcutter ants shows striking parallels with artificially selected plant crops domesticated by humans (e.g., polyploidy, engorged nutritional rewards, dependence on cultivation). To date, poorly resolved L. gongylophorus genomes based on short-read sequencing have constra...
The genus Podaxis was first described from India by Linnaeus in 1771, but several revisions of the genus have left the taxonomy unclear. Forty-four Podaxis species names and nine intraspecific varieties are currently accepted, but most fungarium specimens are labelled Podaxis pistillaris . Recent molecular analyses based on barcoding genes suggest...
Leafcutter ants farm a fungal cultivar (Leucoagaricus gongylophorus) that converts inedible vegetation into food that sustains colonies with up to millions of workers. Analogous to edible fruits of crops domesticated by humans, L. gongylophorus has evolved specialized nutritional rewards—swollen hyphal cells called gongylidia that package metabolit...
Leafcutter ants farm a fungal cultivar (Leucoagaricus gongylophorus) that converts inedible vegetation into food that sustains colonies with millions of workers. Like fruits of crops domesticated by humans, L. gongylophorus has evolved specialized nutritional rewards -tiny swollen hyphal cells called gongylidia that package metabolites eaten by ant...
Symbiosis between insects and fungi arose multiple times during the evolution of both groups, and some of the most biologically diverse and economically important are mutualisms in which the insects cultivate and feed on fungi. Among these are bark beetles, whose ascomycetous cultivars are better known and studied than their frequently-overlooked a...
The taxonomy of Polyporales is complicated by the variability in key morphological characters across families and genera, now being gradually resolved through molecular phylogenetic analyses. Here a new resupinate species, Crystallicutis damiettensis sp. nov. found on the decayed trunks of date palm (Phoenix dactylifera) trees in the fruit orchards...
The taxonomy of Polyporales is complicated by the variability in key morphological characters across families and genera, now being gradually resolved through molecular phylogenetic analyses. Here a new resupinate species, Flavoceraceomyces damiettense (NOM. PROV.) found on the decayed trunks of date palm (Phoenix dactylifera) trees in the fruit or...
Pterulaceae was formally proposed to group six coralloid and dimitic genera [Actiniceps (=Dimorphocystis), Allantula, Deflexula, Parapterulicium, Pterula and Pterulicium]. Recent molecular studies have shown that some of the characters currently used in Pterulaceae Corner do not distinguish the genera. Actiniceps and Parapterulicium have been remov...
Species used in the Russulales analyses and their GenBank accession numbers of nrITS and nrLSU sequences
Questions
Questions (3)
I have a column like the one in the image attached that I've used with sepharose.
I would like to know how to clean them to reuse (with sepharose again).
Hi all,
I'm trying to clean up some DNA for genomic sequencing (Nanopore and Illumina), but i'm still struggling with the purity of my DNA.
My 260/280 ration is ok, that is not an issue, however my 260/230 is always lower than 1.6.
Firstly I thought it could be contaminants carryover from my extract, but I just ran some tests on Lambda phage DNA and got the same problem.
I got some lambda DNA and run some tests with addition of different volumes of my homemade Ampure beads (https://openwetware.org/wiki/SPRI_bead_mix).
I tried 1:1, 1:2 and 1:9 and my 230 ratios were again lower than 1.5.
Is it possible the beads are carrying some PEG? If so it wont be a problem for the libraries preps since the ligation buffers uses peg as well, right?
Can you see other source of 230nm contamination?
Thank you in advance,
Caio
I have extracted DNA from fungal mycelia but when I checked on Nanodrop, most of my samples have a 260/230 ratio < 2.
I think the contamination might be from polysaccharides, since some of the media might have been input in the extraction.
I would like to find the best method for not loose much DNA in the process.
Is it possible to cleanup with Ampure Beads? what would be the ratio sample:beads?
Precipitation of polysaccharides with KAc is a possible option?
Thank you.