Caetano Padial SabinoBiolambda Científica e Comercial LTDA · General Management
Caetano Padial Sabino
PhD
About
57
Publications
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Introduction
Researcher, inventor and entrepreneur, Caetano works since 2010 to help bring photomedicine technologies to mainstream applications. He is PhD in Pharmaceutical Sciences at University of Sao Paulo and also is CEO at BioLambda, a start-up company specialized in innovative equipments for scientific, medical and industrial applications of microbial inactivation mediated by light.
Additional affiliations
January 2016 - present
January 2016 - April 2021
January 2015 - December 2015
Education
January 2012 - December 2015
Publications
Publications (57)
Background:
Photodynamic therapy (PDT) has been long used to treat localized tumors and infections. Currently, microbial inactivation data is reported presenting survival fraction averages and standard errors as discrete points instead of a continuous curve of inactivation kinetics. Standardization of this approach would allow clinical protocols t...
Microbial drug-resistance demands immediate implementation of novel therapeutic strategies. Antimicrobial photodynamic therapy (aPDT) combines the administration of a photosensitizer (PS) compound with low-irradiance light to induce photochemical reactions that yield reactive oxygen species (ROS). Since ROS react with nearly all biomolecules, aPDT...
The global dissemination of the novel coronavirus disease (COVID-19) has accelerated the need for the implementation of effective antimicrobial strategies to target the causative agent SARS-CoV-2. Light-based technologies have a demonstrable broad range of activity over standard chemotherapeutic antimicrobials and conventional disinfectants, neglig...
The rapid and continuous spread of SARS-CoV-2, responsible for COVID-19, has been challenging global health systems and many antimicrobial strategies have been proposed to face the COVID-19 pandemic crisis. In this scenario, ultraviolet lamps emitting ultraviolet C (UV-C) germicidal radiation (peak emission at 254 nm) are in the spotlight to provid...
The unbridled dissemination of multidrug‐resistant pathogens is a major threat to global health and urgently demands novel therapeutic alternatives. Antimicrobial photodynamic therapy (aPDT) has been developed as a promising approach to treat localized infections regardless of drug resistance profile or taxonomy. Even though this technique has been...
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is mainly transmitted by airborne droplets generated by infected individuals. Since this and many other pathogens are able to remain viable on inert surfaces for extended periods of time, contaminated surfaces play an important role in SARS-CoV-2 fomite transmission. Cosmetic products are...
A significant amount of epidemiological evidence has underlined that human-to-human transmission due to close contacts is considered the main pathway of transmission, however since the SARS-CoV-2 can also survive in aerosols, water, and surfaces, the development and implementation of effective decontamination strategies are urgently required. In th...
Background
Bovine digital dermatitis (BDD) is one of the most important diseases that affect dairy cows. Methylene blue-mediated antimicrobial photodynamic therapy (MB-APDT) emerges as a promising technique to treat superficial infections in bovines.
Methods
Twenty BDD lesions located at the skin horn transition of the claw of pelvic limbs of 16 c...
Introduction
The production of β-lactamases by Gram-negative bacteria is among the most important factors of resistance to antibiotics, which has contributed to therapeutic failures that currently threaten human and veterinary medicine worldwide. Antimicrobial photodynamic therapy and antimicrobial blue light have a broad-spectrum antibacterial act...
Introduction
Infections caused by hypervirulent and/or hypermucoviscous Klebsiella pneumoniae strains are frequently reported worldwide. Since convergence of hypervirulence and drug-resistance emerged as a serious clinical problem, novel therapeutic strategies are worthy of investigation. In this regard, antimicrobial photodynamic therapy and blue...
Cutaneous leishmaniasis (CL) is a neglected disease that promotes destructive lesions. Difficulties in treatment are related to accessibility of drugs, resistance and toxicity. Antimicrobial photodynamic therapy (APDT) has been emerging as a promising treatment for CL. In this work, we evaluated methylene blue (MB)-mediated APDT (MB-APDT) on Leishm...
Food safety and quality management play a pivotal role in the dairy industry. Milk is a highly nutritious food that also provides an excellent medium for growth of pathogenic microorganisms. Thus, dairy industry focuses most of their processes and costs on keeping contamination levels as low as possible. Thermal processes for microbial decontaminat...
We report the occurrence and genomic analysis of extended-spectrum β-lactamase (CTX-M)-producing Escherichia coli in anthropogenically polluted coastal waters of Southeast Brazil. E. coli strains belonging to sequence types (STs) ST10, ST38, ST155 and ST1284 exhibited a wide resistome, with genes conferring resistance to medically relevant antimicr...
Background: International clones of multidrug-resistant Escherichia coli have been a leading cause of human and animal infections worldwide. Microbial inactivation by blue light has been proposed as an effective treatment for superficial infections and surface contamination. Aim: To evaluate the inactivation efficacy of blue light irradiation again...
Prototheca spp. are pathogenic algae with important zoonotic potential. Most importantly, these algae often infect dairy cattle. Since there is no effective therapy against the algae, the standard recommendation is the disposal or culling of infected cows to avoid outbreaks. This study investigated the ability of blue light to inactivate pathogenic...
Background:
Cutaneous leishmaniasis (CL) is a vector-borne disease caused by obligate protist parasites from the genus Leishmania. The potential toxicity as well as the increased resistance of standard treatments has encouraged the development of new therapeutical strategies. Photodynamic inactivation (PDI) combines the use of a photosensitizer an...
Background:
Carbapenem-resistant bacterial infections are a critical problem in veterinary medicine with limited treatment options.
Objective:
To describe effective probiotic and photodynamic therapy of a dog with gut colonization and ear infection caused by a hospital-associated lineage of carbapenemase (VIM-2)-producing Pseudomonas aeruginosa....
The emergence and rapid dissemination of colistin-resistant Escherichia coli carrying the plasmid-mediated mcr-1 gene has created an urgent need to develop specific screening methods. In this study, we evaluate four assays based on the inhibition of the MCR-1 activity by EDTA: i) a combined disk test (CDT) comparing the inhibition zones of colistin...
Photodynamic inactivation (PDI) has been reported to be effective to eradicate a wide variety of pathogens, including antimicrobial-resistant microorganisms. The aim of this study was to identify the potential molecular targets of PDI depending on growth phase of Candida albicans. Fungal cells in lag (6 h) and stationary (48 h) phases were submitte...
The emergence and rapid spread of colistin-resistant Escherichia coli carrying the mcr-1 gene has generated an urgent need to strengthen surveillance. We have performed a meticulous investigation of strains of this sort, resulting in the first identification of international clones of E. coli carrying IncX4 plasmid-mediated mcr-1 and blaCTX-M genes...
Candida albicans biofilm is a main cause of infections associated with medical devices such as catheters, contact lens and artificial joint prosthesis. The current treatment comprises antifungal chemotherapy that presents low success rates. Photodynamic inactivation (PDI) involves the combination of a photosensitizing compound (PS) and light to gen...
Systemic inflammation is closely related to the development of insulin resistance and type-2 diabetes, since the activation of pro-inflammatory pathways leads to inhibition of insulin signaling. Although photobiomodulation (PBM) has proven beneficial effects on the treatment of inflammatory disorders, the phototherapeutic approach to manage the chr...
In addition to clinical PDT applications regarding antimicrobial and antineoplastic activity, photodynamic reactions have also been used in several other practices such as for fish tank decontamination, water treatment, antiangiogenic therapy for age-related macular degeneration, decontamination of surfaces, and even inactivation of pathogens for b...
PDT requires a multimodality approach for dosimetry because it works based on three essential components: light, photosensitizer, and molecular oxygen. Since these components are found in variable amounts inside target cells, PDT dosimetry is rather intricate. This chapter intends to address, with little mathematical complexity, the physical and ch...
Photodynamic therapy (PDT) is a clinically approved practice for treatment of cancer and infectious diseases. PDT involves systemic or topical administration of a photosensitizer (PS), followed by irradiation of the target area with light of a wavelength matching the absorption band of the PS. In the presence of oxygen, photochemical reactions trig...
Classical pharmacology is normally concerned with defined molecular structures that can bind to specific proteins and either inhibit or enhance the protein function to achieve some biological response with therapeutic benefit. In photodynamic therapy (PDT) context, we rarely rely on such target specificity to achieve therapeutic success. Although s...
Photodynamic therapy (PDT) rapidly produces large amounts of reactive oxygen species (ROS) to induce death of photosensitized cells. As previously described in Chap. 2, excited photosensitizer (PS) molecules can either donate electrons (type 1) or energy (type 2) to ground-state oxygen to produce superoxide radicals (O2•−) or singlet oxygen (1O2)....
Photodynamic therapy (PDT) was discovered over 100 years ago when it was observed that certain dyes could kill microorganisms when exposed to light in the presence of oxygen. Since those early days, PDT has mainly been developed as a cancer therapy with regulatory approvals and clinical trials steadily accumulating for different types of cancer and...
Nowadays, it is clear that the activity of different photosensitizers (PSs) has a strong potential for moving photodynamic therapy (PDT) to clinical practice. Present technologies as dedicated light sources, new PSs, and nanotechnology are emerging strategies to promote PDT as a reliable, cost-effective, and safe approach to veterinary medicine. Th...
Photodynamic therapy (PDT) harnesses the power of light in an elegant method to produce cytotoxic agents in a spatially and temporally controlled manner and specifically damage target cells and tissues. For photodynamic reactions to occur, the PS molecule must absorb at least one photon to be promoted to a sufficiently long-lived excited state and...
This chapter presents the brightest historical milestones behind the development of photodynamic therapy (PDT). We initially present how photodynamic reactions were first observed by scientists from three different countries in the beginning of the twentieth century. Oskar Raab, from Germany, observed by accident that protozoan cells stained with f...
Our goal was to build, characterize and test a red light-emitting diode (LED) device suitable for wound healing and disinfection of biomedical appliances. We designed and built a unique irradiator metallic box, for which irradiation distribution and spectral irradiance were calculated. In addition, we explored the device's potential in photobiology...
Background: Photodynamic therapy has been investigated in different areas of health through experimental conditions. Its action can alter fundamental structures for the survival of microorganisms without any development of microbial resistance.
Materials and Methods: Young sheep presenting with abscess in the left forelimb caused by Streptococcus s...
Red and near-infrared light have been widely employed in optical therapies. Skin is the most common optical barrier in non-invasive techniques and in many cases it is the target tissue itself. Consequently, to optimize the outcomes brought by lightbased therapies, the optical properties of skin tissue must be very well elucidated. In the present st...
Antimicrobial photodynamic therapy (aPDT), also known as photodynamic inactivation (PDI), photoactivated disinfection (PAD) or photodynamic antimicrobial chemotherapy (PACT), involves the administration of a photoactive dye or photosensitizer (PS) that can produce reactive oxygen species (ROS) upon irradiation with light of the correct wavelength t...
Antimicrobial photodynamic therapy (APDT) combined with endodontic treatment has been recognized as an alternative approach to complement conventional root canal disinfection methods on bacterial biofilms. We developed an in vitro model of bioluminescent Candida albicans biofilm inside curved dental root canals and investigated the microbial reduct...
Pododermatitis is currently one of most frequent and important clinical complications in seabirds kept in captivity or in rehabilitation centers. In this study, five Magellanic penguins with previous pododermatitis lesions on their footpad were treated with photodynamic therapy (PDT). All PDT treated lesions successfully regressed and no recurrence...
Candida spp. are recognized as a primary agent of severe fungal infection in immunocompromised patients, and are the fourth most common cause of bloodstream infections. Our study explores treatment with photodynamic therapy (PDT) as an innovative antimicrobial technology that employs a nontoxic dye, termed a photosensitizer (PS), followed by irradi...
Over the last few years, low-level light therapy (LLLT) has shown an incredible suitability for a wide range of applications for central nervous system (CNS) related diseases. In this therapeutic modality light dosimetry is extremely critical so the study of light propagation through the CNS organs is of great importance. To better understand how l...
has emerged as one of the most important pathogens in healthcare-associated infections worldwide due to its intrinsic and acquired resistance to many antibiotics, including vancomycin. Antimicrobial photodynamic therapy (aPDT) is an alternative therapeutic platform that is currently under investigation for the control and treatment of infections. P...
The objective of this study was to evaluate whether Candida albicans exhibits altered pathogenicity characteristics following sublethal antimicrobial photodynamic inactivation (APDI) and if
such alterations are maintained in the daughter cells. C. albicans was exposed to sublethal APDI by using methylene blue (MB) as a photosensitizer (0.05 mM) com...
Due to the great number of applications of Low-Level-Laser-Therapy (LLLT) in Central Nervous System (CNS), the study of light penetration through skull and distribution in the brain becomes extremely important. The aim is to analyze the possibility of precise illumination of deep regions of the rat brain, measure the penetration and distribution of...
This study analyzed the necessity of use of an optical fiber/diffusor when performing antimicrobial photodynamic therapy (PDT) associated with endodontic therapy. Fifty freshly extracted human single-rooted teeth were used. Conventional endodontic treatment was performed using a sequence of ProTaper (Dentsply Maillefer Instruments), the teeth were...
Several studies indicate that low level laser therapy (LLLT) accelerates the healing process, however, for a determined pathology, dosimetry remains difficult to be established. To understand the tissue optical properties under different conditions is extremely relevant since the dose delivered to the target tissue is known to be critical. The skin...
Candida albicans is the most frequent human opportunistic pathogenic fungus and one of the most important causes of nosocomial infections. In fact, diagnosis of invasive candidiasis presents unique problems. The aim of this work was to evaluate, by fluorescence image analysis, cellular labeling of C. albicans with CdTe/CdS quantum dots conjugated o...
Questions
Questions (2)
I need to quantify and identify oxidative damage on DNA (and proteins). I had some boiling extracts of gram-negative bacteria that showed interesting variation on UV-absorption after an oxidative challenge. I'd like to know how can I interpret this data.
Can anyone guide me, please?
I need to deoxygenate myoglobin samples with cost-effective chemical compounds and read its absorption spectrum.
Projects
Projects (4)
To investigate therapeutic options against multidrug-resistant bacteria based on: i) Synergistic effect resulting from the combination of different antimicrobial compounds; ii) Phage therapy; iii) Bacteriocins; iv) Photodynamic Therapy; vi) Nanoparticles; vii) Antigens and imunoadjuvants; and Drug delivery systems. Galleria mellonella is used as an invertebrate model to study.
The aim of this project is study of in vitro and in vivo photdynamic effect of natural anthraquinones on Cutaneous Leishmaniosis.
