Byung-Soo Chun

• Pukyong National University

This study focused on the valorization of the roots of Saccharina japonica (brown algae) using subcritical water extraction (SSE) and hot water extraction (SHW). The quantities of bioactive compounds from both extracts were also analyzed. The antioxidant and α-glucosidase inhibitory activities of these extracts were evaluated under various extracti...

Algae, widely as a valuable marine biomass, are appreciated globally for their unique chemical compositions and exceptional nutritional benefits. Scientists are increasingly focusing on valorizing algae biomass to recover polysaccharides and bioactive extracts. Conventional methods commonly used to extract bioactive compounds have several limitatio...

The study aimed to identify the optimal subcritical water extraction (SWE) parameters for phlorotannin (marine polyphenols) from Ecklonia stolonifera using response surface methodology (RSM). The reaction temperature, time, and the solid-to-solvent ratio were optimized, and the best extraction conditions were 198.5°C, 36.21 min, and 12.23 g/mL, res...

This study focused on extracting anthocyanin (AC) from purple sweet potato (PSP; Pyrus communis (L.) Lam) using ultrasound-assisted natural deep eutectic solvents (NADES) and incorporating them into biodegradable smart packaging. The effects of water content (40–60%), ultrasound power (40–60%), and extraction time (15–45 min) were optimized using t...

This paper presents an eco-friendly approach to recovering phospholipids (PLs) from the Conger myriaster head (CMH) via supercritical carbon dioxide (SC–CO2) extraction using ethanol as a cosolvent. The lipidomic profile and biological activities of the extracted PLs were investigated. Purity assessments, PL composition analysis, and fatty acid pro...

The subcritical water extraction of Undaria pinnatifida (blade, sporophyll, and root) was evaluated to determine its chemical properties and biological activities. The extraction was conducted at 180 °C and 3 MPa. Root extracts exhibited the highest phenolic content (43.32 ± 0.19 mg phloroglucinol/g) and flavonoid content (31.54 ± 1.63 mg quercetin...

Species of Sargassum genus are known to be rich sources of bioactive compounds. However, there is a lack of studies comparing extraction methods for these bioactive components. This study aimed to compare the total phenolic contents, total antioxidant capacity, tyrosinase inhibitory effect, sargahydroquinoic acid (SHQA) and sargachromenol (SCM), tw...

Marine organisms are an important source of bioactive compounds, among which marine carotenoids have garnered attention for their health benefits. Hundreds of carotenoids are extracted from different natural biomass; among them marine carotenoids always have special appeal due to their unique properties. This review discusses recent literature on m...

Environmental pollution is a significant problem due to the improper disposal of plastics and shrimp shells outdoors. Therefore, the synthesis of biodegradable film from waste materials is highly important. The novelty of this research lies in the extraction of protein hydrolysates and chitosan from shrimp shells, as well as the fabrication of biod...

Phycoerythrin (PE) extract from red algae Laver (Pyropia dentata) was used in the fabrication of a smart packaging film. The PE showed pH sensitivity, implying unique color shifts at different pH. The film colors altered from red through purple-brown to colorless with increasing pH. The smart packaging films were prepared by casting method composed...

This study aimed at the consecutive extraction of neutral and polar lipids (phospholipids) from skipjack tuna (Katsuwonus pelamis) byproducts using an entrainer (ethanol) combination of green extraction system supercritical carbon dioxide. The head, viscera, and skin of tuna fish were subjected to extraction. The highest proportion of lipids was ob...

This study assessed the effectiveness of fatty acid methyl esters (FAME) derived from skipjack tuna (Katsuwonus pelamis) head and viscera oil as a potential antimicrobial agent. The oil was extracted using lipase esterification and Soxhlet extraction methods. The functional pork sausage was then prepared with sodium alginate embedded with fish oil...

This work aims to prepare the functional extracts from Undaria pinnatifida sporophyll using a consecutive green extraction methodology known as supercritical carbon dioxide (SC-CO2) along with subcritical water extraction (SWE). For the SC-CO2 extraction, a variable pressure from 20 to 30 MPa was applied at a constant temperature of 50 °C and compa...

In this study, the smart food packaging films from subcritical water-modified native oat starch (NOS) were developed using betalain from beetroot as a pH biosensor was studied. NOS was modified by SCW at varying temperatures; henceforth modified oats starch (MOS) was designated as MOS@115, MOS@130, MOS@145, MOS@160, MOS@175, and MOS@190. FTIR, XRD,...

This research aimed to valorize brewery spent grain (BSG) using subcritical water hydrolysis (SWH) modified by six different natural deep eutectic solvents (DES) and determining their bipotentialities. DES-induced SWH BSG hydrolysates showed the highest sugar content 46.63 ± 2.10 mg glucose, protein 228.08 ± 5.52 mg BSA, total flavonoids 22.87 ± 0....

Fishery production is exponentially growing, and its by-products negatively impact industries' economic and environmental status. The large amount of bioactive micro-and macromolecules in fishery by-products, including lipids, proteins, peptides, amino acids, vitamins, carotenoids, enzymes, collagen, gelatin, chitin, chitosan, and fucoidan, need to...

Bioactive compounds were extracted from the brown seaweed Ecklonia stolonifera using subcritical water hydrolysis, a green extraction technique to valorize raw biomass. The reaction temperature for the hydrolysis ranged between 120 °C and 220 °C and the products were compared with those obtained by methanol extraction. Reaction temperature played a...

This study aimed to apply protein-rich red algal (Pyropia yezoensis) hydrolysates to formulate functional food using spray drying techniques. Four wall materials (WMs), namely gelatin, whey protein, starch, and dextrin, were used to observe the physicochemical properties and comparative efficiency in spray drying powders (SDP) formation. The effici...

In this study, we characterized the bioactive properties of three important brown seaweed species, Sargassum thunbergii, Undaria pinnatifida, and Saccharina japonica, by subcritical water extraction (SWE), as these species are well known for their beneficial health effects. Their physiochemical properties, including potential antioxidant, antihyper...

This study aimed to prepare gelatin-fucoidan microspheres with enhanced doxorubicin binding efficiency and controllable biodegradation using fish gelatin combined with low molecular weight (LMW) gelatin and fucoidan at fixed ratios. The MW of gelatin was modified by subcritical water (SW), which is known as a safe solvent, at 120 °C, 140 °C, and 16...

Supercritical carbon dioxide (SC-CO2) extraction and subcritical water hydrolysis were utilized to extract the polyunsaturated fatty acid (PUFA) enriched edible oils and amino acid rich hydrolysates from head and viscera of yellow corvina (YC). The omega-3 fatty acid content in the SC-CO2 extracted head oil (SHO) and viscera oil (SVO) was 7.62% and...

Gelatin/carrageenan (Ge/Car) active packaging films incorporated with turmeric essential oil (TEO) encapsulated in zein nanoparticles (ZNP) were developed. The efficacy of these active packaging films and their antimicrobial properties were also investigated to ensure their practical application. Three different types of nanocomposite films (Ge/Car...

Oligochitosan (OC) from shrimp shells derived chitosan was prepared using subcritical water hydrolysis (SWH) and their physicochemical and bio-functional properties were observed in this study. Reaction time of SWH was varied from 15 min to 60 min and the temperature from 140 °C to 170 °C. Gel permeation chromatography (GPC) confirmed that the mole...

Diabetic foot ulcers are imperfections in the process of wound healing due to hyperglycemic conditions. Here, a nanoemulgel fabricated with oregano essential oil nanoemulsion, assisted by low-level laser therapy, was investigated for its efficacy in diabetic wound healing. A hydrogel- based healing patch, fabricated using biological polymers namely...

Among various types children's health challenges, Neuroblastoma is the most serious solid neoplasm forming outside the cranium. S. niphonius is a valuable edible fish that has been widely used for a meal. In this study, we obtained phospholipid extract from the bone of S. niphonius with the supercritical CO2 extraction method and tested anticancer...

Cancer is one of the world's most serious health problems and the top cause of mortality today. S. variolaris is a valuable sea creature that has long been used as a folk medicine to cure and prevent ailments. A subcritical water extraction was used method to obtain crude extract from spikes of S. variolaris. The extract had shown antiproliferative...

We produced squalene-rich cod liver oil (CLO) and solid-lipid particles (SLP) were produced using supercritical carbon dioxide (SC-CO2) extraction and gas saturated solutions (PGSS) processes respectively. Polyethylene glycol (PEG) was used for encapsulation. The encapsulation yield and efficiency (EE) of the SLPs obtained from the oil under 25 MPa...

Bacterial infection is a major complication associated with bioimplant materials, including titanium (Ti) based orthopedic joints and dental implants. Thus, the fabrication of Ti surfaces with antibacterial activity is highly important. Black phosphorus (BP) is a recently discovered promising two-dimensional semiconductor for various biomedical app...

Among various types children’s health challenges, Neuroblastoma is the most serious solid neoplasm forming outside the cranium. S. niphonius is a valuable edible fish that has been widely used for a meal. In this study, we obtained phospholipid extract from the bone of S. niphonius with the supercritical CO 2 extraction method and tested anticancer...

Cod liver oil (CLO) was extracted using conventional hexane and pressing methods or ecofriendly supercritical carbon dioxide (SC-CO2). The extraction main parameters including temperature (A), pressure (B), and CO2 flow rate (C) were optimized using response surface methodology. The oil chemical composition, safety, thermostability, and biological...

Artina pectinata (Comb pen shell, CPS) is a high-protein source that contains a variety of essential amino acids. Subcritical water hydrolysis (SWH) was used to recover amino acids from the posterior adductor muscle (PAM), anterior adductor muscle (ADM), and mantle. The temperatures ranged from 120 °C to 200 °C, and the pressure and time of hydroly...

Artina pectinata (Comb pen shell, CPS) is a high-protein source that contains a variety of essential amino acids. Subcritical water hydrolysis (SWH) was used to recover amino acids from the posterior adductor muscle (PAM), anterior adductor muscle (ADM), and mantle. The temperatures ranged from 120 °C to 200 °C, and the pressure and time of hydroly...

This study investigated the effect of sequential extraction techniques and pretreatment methods in enhancing the recovery of high‐value products from spent coffee ground. Supercritical carbon dioxide (SC‐CO2) deoiling was performed to recover non‐polar lipids, and subcritical water (SCW) hydrolysis of deoiled spent coffee ground (SCG) was conducted...

Green extraction methodologies using supercritical carbon dioxide (SC-CO2) and subcritical water were used to valorize Conger myriaster skin (CMS). The omega-3 fatty acid content in CMS oil extracted using SC-CO2 was 18.62 ± 0.08%, and vitamin A, D, E, and K2 contents were 467.38 ± 0.46 μg/100 g, 8.31 ± 0.02 mg/100 g, 143.42 ± 4.61 mg/100 g, and 1....

Cancer is currently one of the foremost health challenges and a leading cause of death worldwide. Cervical cancer is caused by cofactors, including oral contraceptive use, smoking, multiparity, and HIV infection. One of the major and considerable etiologies is the persistent infection of the oncogenic human papilloma virus. G. applanatum is a valua...

In this study, the optimization of pressurized liquid extraction (PLE) of total phenolic content (TPC), gallic acid (GA), and p-hydroxybenzoic acid (PHBA) from Pseuderanthemum palatiferum (Nees) Radlk. leaves was conducted using response surface methodology. The extract at the optimum conditions of TPC, namely OP, was characterized using GC–MS, HPL...

The aim of this study was to analyze Sargassum thunbergii extracts prepared via subcritical water extraction (SWE). The total maximum contents of phlorotannin, total sugars, and reducing sugars of the S. thunbergii extracts were (13.57 ± 0.19) mg phloroglucinol equivalent (PGE)/g of dry sample, (61.95 ± 0.75) mg glucose/g of dry sample, and (48.51±...

Japanese Spanish mackerel (JSM) (Scomberomorus niphonius) is a marine fish species containing health-beneficial polyunsaturated fatty acids (PUFAs). In the present study, the quality of JSM by-products oils extracted by supercritical CO2 (SC-CO2) and organic solvent extraction was compared in terms of physico-chemical properties of the oils. Eicosa...

Caulerpa racemosa (sea grapes) and Ulva lactuca (sea lettuces) are edible green seaweeds and good sources of bioactive compounds for future foods, nutraceuticals and cosmeceutical industries. In the present study, we determined nutritional values and investigated the recovery of bioactive compounds from C. racemosa and U. lactuca using hot water ex...

In this study, polysaccharides from oyster mushrooms (Pleurotus ostreatus) were extracted using subcritical water (SW) from 120 °C to 200 °C. Polysaccharides obtained at the selected condition that gave the highest yield of the polymer were used for chemical modification. Results showed that SW at 180 °C recovered the highest amount of polysacchari...

The ever-increasing concern about environmental damage caused by plastic waste and its resulting threats on aquatic life has created a greater interest to replace plastics with sustainable and biodegradable alternatives. In this study, packaging film were developed from two abundant wastes, fish skin and spent coffee ground (SCG). The films were pr...

Medical societies and public health agencies rigorously emphasize the importance of adequate disinfection of flexible endoscopes. The aim of this work was to propose a novel opto-chemical disinfection treatment against Staphylococcus aureus grown in mature biofilm on Teflon-based endoscope channel models. Laser irradiation using near-infrared and b...

The present study assesses hepatoprotective effects of raw oyster lyophilized powder (OP) and subcritical water treated oyster powder (SOP) on D-galactosamine (D-GalN)-induced toxicity and acute ethanol intoxication in mice. High-performance liquid chromatography analysis revealed that four phenolic compounds and glucose were identified from the SO...

The cover image is based on our original article 'Edible oil extracted from anchovies using supercritical CO2: Availability of fat-soluble vitamins and comparison with commercial oils' https://doi.org/10.1111/jfpp.15441.

Marine phospholipids (PLs) rich in ω-3 polyunsaturated fatty acids (PUFAs) have drawn keen interest recently among the researchers and consumers, and could be assumed as amazing compounds for pharmacological roles. Due to containing ω-3 PUFAs, marine PLs are highly prone to oxidation in conventional extractions accomplished by the incorporation of...

This study investigated the in vitro bioactivities of extracts obtained from viscera, spines, shells, and gonads of Stomopneustes variolaris using subcritical water extraction (SWE) at 110°C, 150°C, 190°C, and 230°C and Soxhlet extraction. The highest amounts of phenolics (22.68±0.05 mg GAE/g), flavonoids (27.11±0.10 mg RE/g), and proteins (40.25±0...

In this study, oil was extracted from anchovies using supercritical carbon dioxide (SC‐CO2). Additionally, a comparative experiment was conducted with organic solvent‐extracted anchovy oil and commercial fish oil. Omega‐3 fatty acid contents were found to be 32.10±0.45 to 32.15±0.38% for anchovy fish oil. The results of the acid value and the perox...

The following are available online at www.mdpi.com/1660-3397/19/3/137/s1, Table S1. Proximate composition of lyophilized Atrina pectinata powder; Figure S1. Schematic diagram of laboratory scale subcritical water hydrolysis system used in this experiment; Figure S2. Chromatogram of total amino acids from the raw A. pectinata powder; Figure S3. Chro...

The recovery of amino acids and other important bioactive compounds from the comb penshell (Atrina pectinata) using subcritical water hydrolysis was performed. A wide range of extraction temperatures from 140 to 290 °C was used to evaluate the release of proteins and amino acids. The amount of crude protein was the highest (36.14 ± 1.39 mg bovine s...

For tissue engineering applications, biodegradable scaffolds containing high molecular weights (MW) of collagen and sodium alginate have been developed and characterized. However, the properties of low MW collagen-based scaffolds have not been studied in previous research. This work examined the distinctive properties of low MW collagen-based scaff...

A sequential recovery of free amino acids from blue mussel (Mytilus edulis) by subcritical water after deoiling was performed. Hydrolysis temperatures ranged from 120 °C to 240 °C, pressure and reaction time were fixed at 3 MPa and 30 min, respectively. Results showed that hydrolysis efficiency reached 90.65% at 240 °C. Total amino acids in the lyo...

The present work focuses on astaxanthin (ASX) extraction from shrimp wastes using ultrasound-assisted (UAE) natural deep eutectic solvents (NaDESs) and the utilization of ASX-rich NaDES extracts (ASX-NaDESs) for preparing biodegradable active packaging. The effects of hydrogen bond donor (HBD)/ acceptor (HBA) molar ratio, ultrasound amplitude, extr...

In this study, subcritical water was used to crosslink gelatin and alginate (G/A) at different temperatures (120 °C, 130 °C, 140 °C, 150 °C, and 160 °C) under constant pressure and reaction time (3 MPa, 30 min). Structural and morphological characterization was performed using Fourier-transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD)...

Sea urchin gonads are a high-priced delicacy across Southeast Asia. These gonads make up a small portion of the total body mass of sea urchins. The remaining parts, like the shells, spines and viscera are discarded as waste. Recent research has used conventional extraction techniques to demonstrate the bioactive potential of these sea urchin byprod...

Every year, a large amount of by‐products produced by fish processing industries is discarded or used for less profitable purposes. In this study, we will describe a technique for producing high‐quality, edible oil from the viscera of yellowtail fish (Seriola quinqueradiata) which can be served as a source of omega polyunsaturated fatty acids. Yell...

Recently, green synthesis of silver/silver chloride nanoparticles (Ag/AgCl-NPs) has gained a lot of interest because of the usage of natural resources, rapidness, eco-friendliness, and benignancy. Several researchers reported that silver-based biogenic NPs have both antimicrobial and anticancer properties. In the present study, Ag/AgCl-NPs were syn...

This study aims to compare the biofunctional properties of wild cultivated ginseng and cultivated ginseng extracts obtained by subcritical water extraction. The compounds that contribute to the biofunctional properties of ginseng extracts were analyzed by different conditions (100°C/40 bar–240°C/90 bar). When the temperature was increased, ginsneg...

In the present study, astaxanthin (ASX)-enriched oil extracted from Tiger shrimp waste, Peneaus monodon, and Japanese Spanish mackerel, Scomberomous niphonius, skin in concurrent extraction by supercritical carbon dioxide and quality properties of the oil was evaluated. The extraction condition was optimized by response surface methodology to obtai...

Achatina fulica, which is one of the land snails with high protein content, was decomposed by compressed hot water at various temperatures (100 °C–300 °C) and characterized its physicochemical properties, Angiotensin I-converting enzyme (ACE) inhibitory, Acetylcholinesterase (AChE) inhibitory, and antioxidant activities. The physical properties of...

The research work was aimed to produce 2-monoacylglycerol rich omega-3 polyunsaturated fatty acids from supercritical carbon dioxide (SC-CO2) extracted Atlantic salmon frame bone oil (SFBO) by using sn-1,3 lipase catalyzed ethanolysis. SFBO was extracted by SC-CO2 at 45 °C, 25 MPa at carbon dioxide flow rate of 27 g/min for 3 h. Four sn-1,3 specifi...

Seaweed is consumed as nutritive food materials in many Asian countries such as Japan, Korea and China. In the present study, we analyzed proximate composition of two Korean seaweeds Sargassum horneri and Ecklonia cava . Crude protein content of S. horneri and E. cava were 8.57% and 9.11%, whereas 1.03% and 1.40% of total lipid was found from S. ho...

This study was aimed at isolation of pepsin-solubilised collagen (PSC) from Mackerel (Scomber japonicus) bone and skin in order to effectively valorise these abundant wastes. The yield of PSC (8.10%) from skin was considerably higher than that from bone (1.75%). Based on the protein patterns, both PSCs were type Ι, and consisted of two α-chains. Fo...

Bacterial collagenolytic protease-extracted Bigeye tuna skin collagen was subjected to catalysts-assisted subcritical water hydrolysis. The degree of hydrolysis was the highest for hydrolysates obtained using sodium bicarbonate catalysts. The color and pH values of the hydrolysates varied depending of the catalysts used. Four different assays of an...

Hypnea musciformis is an edible tropical red seaweed species and still considered as an underexploited seaweed species in Indonesia. In this study, H. musciformis was hydrolyzed using the subcritical water extraction (SCWE) system. Different solid to liquid ratios (S/L ratios 1:150, 1:100, and 1:50) and six different temperature conditions (120–270...

Fish collagen is gaining immense interest because the use of mammalian collagen is restricted due to disease transmission and religious issues. So, collagen was extracted and characterized from three different parts (skin, scale, and bone) of bigeye tuna using the acid and pepsin extraction methods. The yield of acid-soluble collagen (ASC) and peps...

The present study investigated the recovery of antioxidative compounds obtained from Pseuderanthemum palatiferum (Nees) Radlk. and their biological activities using subcritical water in comparison with hot water, Soxhlet, and methanol extraction. In this study, high‐performance liquid chromatography (HPLC) and three commonly used assays, that is, 2...

This study aimed to create P. yezoensis hydrolysates, via subcritical water hydrolysis (SWH), which can enhance food functionality. P. yezoensis hydrolysates were produced at (120 to 230) °C and 30 bar. Extraction was performed using hot water and ethanol for comparison. The maximum radical-scavenging activity of DPPH and ABTS was (16.63 ± 0.20) mg...

Pacific oyster muscle hydrolysates were prepared by pressurized hot water extraction (PHWE) at process conditions ranging 125 °C/20 bar 275 °C/140 bar to investigate various physico-chemical and bio-functional parameters. Degree of hydrolysis was increased with increasing temperature and pressure and at 275 °C/140 bar, hydrolysis was found to be hi...

Penaeus japonicus hydrolysates were produced by hot-compressed water treatment at 100 °C – 200 °C and 30 bar for 10 min. Hydrolysate yield varied from 44.87 ± 1.74% to 88.55 ± 2.15% with the maximum yield obtained at 200 °C. Hydrolysates showed alkaline pH, which incrementally increased with temperature. Protein, phenolic compounds, and Maillard re...

Various catalysts such as formic acid, acetic acid, sodium hydroxide, sodium bicarbonate, carbon dioxide and nitrogen gas were used for hydrolyzates production from deoiled mackerel muscle by subcritical water hydrolysis. Above 99% hydrolysis yield was obtained using sodium bicarbonate catalyst at 260 °C/70 bar. Histamine and heavy metals concentra...

Emulsion-based delivery systems have been fabricated and developed to increase the bioavailability of astaxanthin and alpha-tocopherol as active compounds for various biomedical applications. Astaxanthin-alpha tocopherol nanoemulsion (ATNE) is well known for its potential 6.-6.30 effect. The current study investigated ATNE by spontaneous (SENE) and...

A nanocomposite film, chitosan (CS)-polyvinylpyrrolidone (PVP)-bentonite (BN) was fabricated to enhance wound healing processes as a new nanoplatform for wound dressing. Both physical properties and antibacterial activity of the proposed film were examined to validate its applicability and inhibitory effect for wound management. In vitro cytotoxici...

Herein, deep eutectic solvent combined with subcritical water extraction of seaweed polysaccharides were investigated from Saccharina japonica. In this regard, a deep eutectic solvent was added with sufficient amount of water and it was used as an extraction medium. To optimize, a Box–Behnken design was used to study the influences of the temperatu...

Fucoidan is a marine sulfated polysaccharide that possesses various biological activities. To enhance the functional properties of fucoidan, it was depolymerized using a green technique viz. subcritical water treatment (SCW) to produce a low molecular weight fucoidan. In this study, response surface methodology (RSM) was used to study the influence...

Fucoxanthin is a precious marine carotenoid with multiple bioactivities such as antioxidant, anti-obesity, and anti-cancer effects. Despite its valuable properties, fucoxanthin possesses high sensitivity when exposed to aerial, thermal, or light factors. Our study aims to extract fucoxanthin-rich oil (FO) from Saccharina japonica using supercritica...

In this study, chitin was exclusively extracted from shrimp shells (Marsupenaeus japonicas) through a green solvent called deep eutectic solvent (DES), and various types of DES were utilized to extract chitin. The physicochemical properties of the obtained chitin were compared with the conventional method. A high purity of chitin was obtained while...

Salmon oil rich in omega-3 polyunsaturated fatty acids and astaxanthin was microencapsulated in polyethylene glycol-6000 using particles from gas saturated solutions process. The process conditions viz. temperature (45–55 °C), pressure (15–25 MPa), oil and polymer ratio (1:2.5–1:10), and nozzle size (300–500 μm) were optimized for maximizing encaps...

Subcritical water hydrolysis was applied to obtain antioxidant and antimicrobial hydrolysates from tuna skin and isolated collagen. Different temperatures (150–300 °C) with a pressure (50–100 bar) and reaction time (5 min) were employed to find the optimum condition. Degree of hydrolysis was highest at 250 °C for both skin hydrolysate (SH) and coll...

Marine (PLs) are rich in omega-3 polyunsaturated fatty acids (ω-3 PUFAs), and the good emulsifying property of PLs makes them suitable for use in the food, pharmaceutical, and cosmetic industries. This research was attempted to evaluate Atlantic salmon frame bone (SFB) PLs extracted by using supercritical carbon dioxide (SC-CO2) with ethanol as co-...

Subcritical water extraction (SWE) of fucoidan from Saccharina japonica was investigated. To optimize, a Box-Behnken design (BBD) was used to study the influences of temperature [100–180 °C], pressure [20–80 bar], solid to liquid (S/L) ratio [0.04–0.09 g mL⁻¹], agitation speed (AS) [100–300 rpm], and reaction time (RT) [5–15 min]. By unraveling the...

In this study, marine tunicate Styela clava hydrolysate was produced by an environment friendly and green technology, pressurized hot water hydrolysis (PHWH) at different temperatures (125–275 °C) and pressure 50 bar. A wide range of physico-chemical and bio-functional properties such as color, pH, protein content, total carbohydrate content, reduc...

The separation of oil by a suitable technique from the Pacific oyster muscle is important for the utilization of the oil as a ω-3 polyunsaturated fatty acids (ω-3 PUFAs) source and production of bio-functional peptides/ oligosaccharides from oil-free residue. This study was conducted to prepare ω-3 PUFAs concentrate from supercritical carbon dioxid...

Collagen extracted from fish skin by using porcine-derived pepsin is not accepted by a large number of people due to religious constrains. The aim of the study was to explore bacterial collagenolytic proteases (CP) to extract fish skin collagen and characterize the extracted collagen. Two bacteria, Bacillus cereus FORC005 and Bacillus cereus FRCY9-...