Bushra Jamil

BJ Micro Lab (SMC Private) Limited

Community-associated, methicillin-resistant Staphylococcus aureus (MRSA) lineages have emerged in many geographically distinct regions around the world during the past 30 y. Here, we apply consistent phylodynamic methods across multiple community-associated MRSA lineages to describe and contrast their patterns of emergence and dissemination. We gen...

Community-associated, methicillin-resistant Staphylococcus aureus (MRSA) lineages have emerged in many geographically distinct regions around the world during the past 30 y. Here, we apply consistent phylodynamic methods across multiple community- associated MRSA lineages to describe and contrast their patterns of emergence and dis- semination. We...

Purpose This study aims to investigate new tissue conditioner (TC) formulations involving chitosan nanoparticles (CSNPs) and essential oils (EO) for their antifungal potential, release kinetics, and hardness. Materials and methods CSNPs were synthesized, and the separate solutions of CSNP were prepared with two types of EO, i.e., Oregano oil and L...

Staphylococcus aureus is an important healthcare-associated bacterium that causes a multitude of infections in humans such as superficial skin and soft tissue infections, necrotizing pneumonia, foodborne illnesses and postsurgical infections. Treatment of S. aureus infections has become more complicated due to the emergence of Methicillin-Resistant...

Community-associated, methicillin-resistant Staphylococcus aureus (MRSA) lineages have emerged in many geographically distinct regions around the world during the past 30 years. Here, we apply consistent phylodynamic methods across multiple community-associated MRSA lineages to describe and contrast their patterns of emergence and dissemination. We...

Burn injuries are the most prevalent and devastating form of skin trauma. Current study aimed to fabricate novel chitosan-based composite films of vancomycin for wound healing applications. The developed vancomycin-chitosan films were evaluated for various quality attributes and were subjected to anti-bacterial activity against methicillin resistan...

This investigation is vital contribution to the healthcare system utilizing techniques of nanobiotechnology. It interestingly applies chitosan capped CuO nanoparticles in the field of medicine and restorative dentistry. The CuO nanoparticles and CuO-Chitosan nanoparticles are prepared by co-precipitation, and their characterization is performed usi...

Antibiotic resistance is a health challenge worldwide. Carbapenem resistance in Gram-negative bacteria is a major problem since treatment options are very limited. Tigecycline and colistin are drugs of choice in this case, but resistance to these drugs is also high. The aim of this study was to describe the diversity of resistance mechanisms in car...

Rapidly emerging drug resistance in all classes of pathogenic microorganisms has become a challenging task and a global health issue in recent years. There are very limited alternative options available to cure infectious diseases, as the rate of rise in drug resistance in infectious agents is higher than the arrival of new antimicrobial drugs. The...

Objective: The purpose of the study was to assess bacteriological quality of drinking water in Rawalpindi and Islamabad-Pakistan by determining the presence of coliforms and E.coli. Material and Methods: This cross-sectional descriptive study was conducted at the Department of Microbiology, Armed Forces Institute of pathology, Rawalpindi from Sep 2...

Theranostic approaches have been suggested for various ailments, particularly cancer, microbial diseases, AIDS, and many others. This is a kind of personalized treatment where the treatment is guided according to the individual molecular profile or on the basis of biomarker identification. Combination of diagnostics and therapeutic strategy into a...

Worldwide the infectious diseases are a major threat to the human population. However, the most important parameter is the prompt and sensitive diagnosis of pathogens. Therefore, tremendous efforts have been made for the development of rapid and portable detection techniques. The identification and treatment of infectious diseases at the nano and m...

Escalating antibiotic resistance has warranted to develop strategies for appropriate use of antibiotics. Antimicrobial stewardship (AMS) seems therefore a need of the hour. Misuse, abuse and overuse of available antimicrobials is the pivotal cause of decline in antibiotics’ efficiency. Development of resistance to treatment of last resort have forc...

This book is specifically designed to provide information about various nanocarriers developed so far under nanotheranostics for a sustained, controlled and targeted co-delivery of diagnostic and therapeutic agents. In addition to that diverse theranostic application of nanotechnology and the limitations to attain the goal is also addressed. It int...

Objective: To determine the prevalence of resistant pathogens and their antimicrobial susceptibility pattern in an intensive care unit. Methods: The cross-sectional observational study was conducted at Foundation Hospital, Rawalpindi, Pakistan, from May to September 2016, and comprised tracheal tubes which were collected in sputum culture bottle...

Eggs are a healthy and nutritious food source, but may be contaminated by bacteria. Previous studies have reported the presence of staphylococci in eggs of farmed chickens, but no study has evaluated the staphylococcal population of eggs from household chickens. In this study, staphylococci from eggs (n=275) of household chickens collected from Nov...

Patients with end-stage renal disease (ESRD) are immunocompromised and are more at risk to develop and acquire Mycobacterium tuberculosis (MTB) infection. However, risk assessment is uncertain. The objective of current research was to study the frequency of MTB infection in ESRD patients . For this purpose, bronchoalveolar lavage (BAL) samples were...

It is believed that the future of medicine lies in nanotechnology where the medicines would be tailor-made according to the requirement of the individual. With the amassed recognition of the potential of nanomaterials they are not only used in medicines but in many other consumer products. Nanomaterials exists in nature everywhere and many others a...

Aim: A DNA microarray-based assay for the detection of antimicrobial resistance (AMR) genes was used to study carbapenemase-producing organisms at the Kidney Center of Rawalpindi, Pakistan. Methods: The evaluation of this assay was performed using 97 reference strains with confirmed AMR genes. Testing of 7,857 clinical samples identified 425 Gra...

ST239-MRSA-III is probably the oldest truly pandemic MRSA strain, circulating in many countries since the 1970s. It is still frequently isolated in some parts of the world although it has been replaced by other MRSA strains in, e.g., most of Europe. Previous genotyping work (Harris et al., 2010; Castillo-Ramírez et al., 2012) suggested a split in g...

The global emergence of metallo-β-lactamase (MBL) producing bacterial pathogens has rendered the current therapeutic options ineffective. This perturbing situation requires an inventive strategy to stop the dissemination of MBL producers and their associated infections. The present investigation reports the fabrication and evaluation of bio-based n...

The prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in Pakistan is known to be high, but very few studies have described the molecular epidemiology of the different MRSA clones circulating in the country. Forty-four MRSA isolates were collected from two tertiary care hospitals of the Rawalpindi district of Pakistan. All strains wer...

Multidrug resistance (MDR) in gram-negative pathogens is the emerging threat to clinicians. The current study was designed to determine the prevalence and pattern of multidrug resistance in gram-negative clinical isolates. It was conducted at the COMSATS Institute of Information Technology, Islamabad, Pakistan, from June to October 2014. Of the 8,...

SCCmec (staphylococcal cassette chromosome mec) elements are large, mobile genetic elements in staphylococci that carry methicillin resistance genes mecA or mecC. Additionally, they harbor recombinase and accessory genes. Twelve main SCCmec types and several variants have been described based on the identity of the mec complex and of the ccr recomb...

Background: Tremendous efforts through dedicated research have been made for the development of robust, point of care, portable, cost effective, sensitive and specific assays of microbial detection. Biosensing and nanotechnology offer novel strategies of designing state of the art devices and assay formats with enhanced sensitivity and all other d...

Escalating resistance to almost every class of antibiotics is reducing the utility of currently available antimicrobial drugs. A part of this menace is attributed to poor pharmacokinetics and pharmacodynamics of the drug. Improvement in drug delivery is the most challenging task encountered by the pharmaceutical industries; however, nanotechnology...

Table eggs are nutritionally important food consumed globally. Despite being protected inside the hard shell and a semipermeable membrane, the egg contents may be contaminated with microbes and thus become a possible carrier of infectious agents to humans. A number of medically significant bacterial species such as Salmonella enterica, Listeria mon...

Objective: To determine Candida colonisation/infection in renal transplant patients and to determine the resistance pattern against antifungal drugs. Methods: This prospective, observational study was conducted at Al-Sayyed Hospital, Rawalpindi, Pakistan, from January to October 2014, in collaboration with the Microbiology and Public Health Labo...

Background: Rapid molecular identification of resistance genes in Gram-negative bacteria is crucial for infection control and prevention, for surveillance and for epidemiological purposes. Furthermore, it may have a significant impact on determination of the appropriate initial treatment and thus it can greatly benefit critically ill patients. Mat...

Understanding the interplay between bacterial pathogens and antimicrobials is a key to realize the control over infections causing morbidity and mortality. An important current issue of contemporary medicine and microbiology is the search for new strategies for adequate therapy of infectious diseases associated with rapidly emerging multidrug-resis...

Antimicrobial resistance presents a significant challenge to scientists in the field of infectious diseases. The full knowledge of how antibiotics resistance is evolving and being transmitted between hosts in different ecosystems is taking on great importance. Necessary action includes research to define the scope of the problem including its vario...

Natural antimicrobial agents, particularly essential oils present an excellent alternative to current antibiotics due to their potent and broad-spectrum antimicrobial potential, unique mechanisms of action and low tendency to induce resistance. However their potential as a viable therapeutic alternative is greatly compromised due to their hydrophob...

Objective: To determine the prevalence of bacteraemia in patients with chronic kidney disease, particularly those on dialysis and those who had had renal transplant, and to evaluate resistance among the isolated strains. Methods: The cross-sectional study was conducted at Kidney Centre, Al-Sayyed Hospital, Rawalpindi, Pakistan, from June to Dece...

Bordetella parapertussis is the causative agent of a milder form of pertussis or whooping cough. Little is reported about the antibiotic resistance patterns and mechanism of drug resistance of Bordetella parapertussis. The objective of this study has been to investigate antimicrobial resistance, distribution of integrons and presence of gene casset...

Frequent incidents of antibiotic-resistant biofilm forming pathogens in community-associated and hospital-acquired infections have become a global concern owing to failure of conventional therapies. Nano-antibiotics (NABs) are de novo tools to overcome the multi-drug resistant mechanisms employed by the superbugs. Inhibition of biofilm formation is...

Escalating resistance to almost every class of antibiotics is reducing the utility of currently available antimicrobial drugs. A part of this menace is attributed to poor pharmacokinetics and pharmacodynamics of the drug. Improvement in drug delivery is the most challenging task encountered by the pharmaceutical industries; however nanotechnology c...

The genus Bacillus produces mainly polypeptide antibiotics such as bacitracin and polymyxin. Bacillus species were isolated from soil by soil sprinkle technique. And all were screened for the production of antibiotic. Bacillus subtilis MH-4 gave the maximum antimicrobial activity so finally selected for optimization. During optimization of culture...