Bunyamin Tar'an

University of Saskatchewan | U of S · Department of Plant Sciences

With the expanding interest in plant-based proteins in the food industry, increasing emphasis is being placed on breeding for protein concentration and quality. Two protein quality traits i.e., amino acid profile and protein digestibility, were assessed in replicated, multi-location field trials from 2019 to 2021 in pea recombinant inbred line popu...

The warming Canadian summers have become a major abiotic stress to crops including pea. In the past decade, attempts were made in the understanding of heat stress effect and genomic mapping for heat responsive traits in field pea. In this study, a new recombinant inbred line population (PR-24) consisting of 39 lines was tested in six trials in the...

Context The process of biological nitrogen fixation (BNF) through nodules of legumes benefits soil fertility, increases crop productivity and reduces the requirement for chemical fertilizers. Pea is an important pulse crop in temperate regions of the world. The N fixation capacity of pea in Canadian environments ranges from 50% to 55% of crop N req...

Heat shock proteins (HSPs) and abscisic acid (ABA) play important roles in plant heat responses, but have not been extensively studied in pea (Pisum sativum L.), whose heat susceptibility is well‐known. In this study, four pea varieties varying in heat tolerance based on field trials were evaluated. Plants were heat stressed for 3 h, 6 h, 12 h or 2...

This research aimed to identify quantitative trait loci (QTLs) associated with seed protein concentration in a recombinant inbred line (RIL) population of pea and aimed to validate the identified QTLs using chromosome segment-introgressed lines developed by recurrent backcrossing. PR-25, an RIL population consisting of 108 F7 bulked lines derived f...

Chickpea is a cool season crop that is highly vulnerable to abiotic stresses such as heat and drought. High temperature during early flowering and pod development stages significantly reduces the crop yield. The wild relatives of chickpeas can be potential donors for the introgression of heat and drought tolerance into cultivated chickpeas for crop...

CDC Hickie, a yellow cotyledon field pea (Pisum sativum L.) cultivar, was released in 2021 by the Crop Development Centre, University of Saskatchewan for distribution to Select seed growers through the Variety Release Committee of the Saskatchewan Pulse Growers. CDC Hickie has good lodging resistance, medium time to maturity, medium-sized, round se...

CDC Lewochko, a yellow cotyledon field pea (Pisum sativum L.) cultivar, was released in 2018 by the Crop Development Centre, University of Saskatchewan for distribution to Select seed growers through the Variety Release Committee of the Saskatchewan Pulse Growers. CDC Lewochko has good lodging resistance, medium time to maturity, medium-sized, roun...

CDC Tollefson, a yellow cotyledon field pea (Pisum sativum L.) cultivar, was released in 2021 by the Crop Development Centre, University of Saskatchewan for distribution to Select seed growers through the Variety Release Committee of the Saskatchewan Pulse Growers. CDC Tollefson has good lodging resistance, medium time to maturity, medium-sized, ro...

Zero hunger and good health could be realized by 2030 through effective conservation, characterization and utilization of germplasm resources ¹ . So far, few chickpea ( Cicer arietinum ) germplasm accessions have been characterized at the genome sequence level ² . Here we present a detailed map of variation in 3,171 cultivated and 195 wild accessio...

Field pea ( Pisum sativum L.), a cool-season legume crop, is known for poor heat tolerance. Our previous work identified PR11-2 and PR11-90 as heat tolerant and susceptible lines in a recombinant inbred population. CDC Amarillo, a Canadian elite pea variety, was considered as another heat tolerant variety based on its similar field performance as P...

Canning or boiling pulse seeds in water produces a by-product solution, called “aquafaba”, that can be used as a plant-based emulsifier. One of the major problems facing the commercialization of aquafaba is inconsistency in quality and functionality. In this study, chickpea aquafaba production and drying methods were optimized to produce standardiz...

Legumes are an excellent source of proteins and health‐promoting phytochemicals. Recognizing their importance in human nutrition and sustainable agricultural production, significant efforts are currently being made to accelerate genetic gain related to yield, stress resilience, and nutritional quality. Recent increases in genomic resources for mult...

Field pea ( Pisum sativum L.), a cool-season legume crop, is known for poor heat tolerance. Our previous work identified PR11-2 and PR11-90 as heat tolerant and susceptible lines in a recombinant inbred population. CDC Amarillo, a Canadian elite pea variety, was considered as another heat tolerant variety based on its similar field performance as P...

We report here the synthesis and biological testing of 3'-(phenyl alkynyl) abscisic ABA analogs, a new class of potent ABA antagonists. These ABA analogs incorporate a rigid framework of eight carbon atoms attached at the 3'-carbon atom of ABA that prevents folding of the ABA analog-bound receptor required for ABA signalling. The two-step synthesis...

አህፅሮት የዚንክ ንጥረ-ይዘት ለሚያጥርባቸው የእርሻ መሬት የዚንክ ማዳበሪያ መጨመር በምርትም ሆነ በምርት የዚንክ ንጥረ-ይዘት ላይ አወንታዊ እመርታ እንደሚያሰገኝ ይታመናል፡፡ ሆኖም የሚጨመረውን የማዳበሪያ መጠን የሚወስነዉ የአፈር ውስጥ የዚንክ ንጥረ ይዘት እጥረት መጠን፣ የአፈሩ ዓይነት፣ የስብል ዓይነትና ዝርያ እንዲሁም የአጨማመር ዘዴ ነዉ፡፡ የዚህ ጥናት ዓላማ የሽምብራን ምርትና የምርት ዚንክ ንጥረ-ይዘት መጠንን የሚያሻሽል የዚንክ ማዳበሪያ መጠንን ለዚንክ ንጥረ-ይዘት መጠን አነስተኛ ለሆነ መሬት ለመወሰን ነው፡፡ የአፈር ዚንክ መጠን ዝቅተኛ...

ABA antagonists have potential application as growth regulators to improve germination and seedling growth at low temperatures for oilseeds and pulses grown in regions with short seasons such as those in western Canada. Towards development of practical ABA antagonists, a series of 3′-substituted ABA analogs were synthesized and screened in seed ger...

Chickpea is a widely produced pulse crop, but requires processing prior to human consumption. Protein bioavailability and amino acid quantity of chickpea flour can be altered by multiple factors including processing method. For this reason, the protein quality of processed chickpea flour was determined using in vivo and in vitro analyses for proces...

Key message: Integration of genomic technologies with breeding efforts have been used in recent years for chickpea improvement. Modern breeding along with low cost genotyping platforms have potential to further accelerate chickpea improvement efforts. The implementation of novel breeding technologies is expected to contribute substantial improveme...

Folates are important metabolic cofactors essential for human growth and development. Eighty-five accessions from a pea genome wide association study (GWAS) panel were evaluated for folate profile using ultra-performance liquid chromatography coupled with mass spectrometry. A GWAS was conducted to identify SNP markers associated with the folate pro...

Genome-wide association study (GWAS) was conducted to identify loci associated with agronomic (days to flowering, days to maturity, plant height, seed yield and seed weight), seed morphology (shape and dimpling), and seed quality (protein, starch, and fiber concentrations) traits of field pea (Pisum sativum L.). A collection of 135 pea accessions f...

Core ideas: Quantitative trait locus (QTL) analyses for carotenoids in chickpea were completed for three F2 populations. A moderate number of QTLs and candidate genes associated with carotenoid concentration in chickpea seeds were identified. Green cotyledon color is positively associated with provitamin A carotenoids. Three F2 populations derived...

Iron (Fe) deficiency is one of the most common nutritional disorders, and is mainly due to insufficient intake of bioavailable Fe. Chickpea (Cicer arietinum L.) was examined as a potential vehicle for Fe fortification. Fortificants (FeSO4·7H2O (ferrous sulfate hepta-hydrate), FeSO4·H2O (ferrous sulfate mono-hydrate) and NaFeEDTA (ethylenediaminetet...

We report the first annotated chromosome-level reference genome assembly for pea, Gregor Mendel’s original genetic model. Phylogenetics and paleogenomics show genomic rearrangements across legumes and suggest a major role for repetitive elements in pea genome evolution. Compared to other sequenced Leguminosae genomes, the pea genome shows intense g...

Key message A high-density linkage map of chickpea using 3430 SNPs was constructed and used to identify QTLs and candidate genes for ascochyta blight resistance in chickpea. Abstract Chickpea cultivation in temperate conditions is highly vulnerable to ascochyta blight infection. Cultivation of resistant cultivars in combination with fungicide appl...

Unfortunately there was an error in the name of the gene “Ca-AKL18” in the discussion section.

Whole genome profiling (WGP) is a sequence-based physical mapping technology and uses sequence tags generated by next generation sequencing for construction of bacterial artificial chromosome (BAC) contigs of complex genomes. The physical map provides a framework for assembly of genome sequence and information for localization of genes that are dif...

Unique sequence tags identified by sequencing ends of restriction fragments of 295,680 BAC clones.

Number of BAC clones in each contig built based on the sequence similarities of unique sequence tags.

Distribution of BAC clones in contigs built based on the sequence similarities of unique sequence tags.

Fingerprinted Contig output file to visualize all the BAC contigs and overlap of each BAC in the reported contigs.

BAC clones corresponding to the unique sequence tags identified by sequencing ends of restriction fragments of 295,680 BAC clones.

Whole genome profiling (WGP) is a sequence-based physical mapping technology and uses sequence tags generated by next generation sequencing for construction of bacterial artificial chromosome (BAC) contigs of complex genomes. The physical map provides a framework for assembly of genome sequence and information for localization of genes that are dif...

Figure S1 Frequency distribution ascochyta blight disease scores in 92 RILs of CPR‐01 population developed from a cross between ICCV 96029 and CDC Frontier.

Table S1 Summary of analysis of variance for ascochyta blight scores in the CPR‐02 RILs tested under greenhouse (combined data of two experimental repeats of three replications each) and field (combined and individual data from field conditions at Elrose, Saskatchewan, Canada in 2014 and 2015). Table S2 Summary of QTLs for resistance to ascochyta...

Whole genome profiling (WGP) is a sequence-based physical mapping technology and uses sequence tags generated by next generation sequencing for construction of bacterial artificial chromosome (BAC) contigs of complex genomes. The physical map provides a framework for assembly of genome sequence and information for localization of genes that are dif...

Background: The objective of this research was to map quantitative trait loci (QTLs) of multiple traits of breeding importance in pea (Pisum sativum L.). Three recombinant inbred line (RIL) populations, PR-02 (Orb x CDC Striker), PR-07 (Carerra x CDC Striker) and PR-15 (1-2347-144 x CDC Meadow) were phenotyped for agronomic and seed quality traits...

Whole‐genome sequencing‐based bulked segregant analysis (BSA) for mapping quantitative trait loci (QTL) provides an efficient alternative approach to conventional QTL analysis since it significantly reduces the scale and cost of analysis with comparable power to QTL detection using full mapping population. We tested the application of next‐generati...

Practical application: Iron fortification of dehulled lentil dal may change organoleptic attributes that can influence consumer acceptability. Sensory evaluation by consumers helps to determine the effect on appearance, odor, taste, texture, and overall acceptability of fortified lentils. In this study, consumer acceptability was evaluated with pa...

Domesticated species are impacted in unintended ways during domestication and breeding. Changes in the nature and intensity of selection impart genetic drift, reduce diversity and increase the frequency of deleterious alleles. Such outcomes constrain our ability to expand the cultivation of crops into environments that differ from those under which...

Plant roots host symbiotic arbuscular mycorrhizal (AM) fungi and other fungal endophytes that can impact plant growth and health. The impact of microbial interactions in roots may depend on the genetic properties of the host plant and its interactions with root-associated fungi. We conducted a controlled condition experiment to investigate the effe...

CDC Inca, a yellow cotyledon field pea (Pisum sativum L.) cultivar, was released in 2015 by the Crop Development Centre, University of Saskatchewan for distribution to select seed growers through the Variety Release Committee of the Saskatchewan Pulse Growers. CDC Inca has good lodging resistance, medium-sized round seeds, and good yielding ability...

CDC Spectrum, a yellow cotyledon field pea (Pisum sativum L.) cultivar, was released in 2016 by the Crop Development Centre, University of Saskatchewan for distribution to select seed growers through the Variety Release Committee of the Saskatchewan Pulse Growers. CDC Spectrum has good lodging resistance, medium-sized round seeds, and good yielding...

CDC Spruce, a green cotyledon field pea (Pisum sativum L.) cultivar, was released in 2016 by the Crop Development Centre, University of Saskatchewan for distribution to select seed growers through the Variety Release Committee of the Saskatchewan Pulse Growers. CDC Spruce has good lodging resistance, medium-sized round seeds, and good yielding abil...

CDC Canary, a yellow cotyledon field pea (Pisum sativum L.) cultivar, was released in 2017 by the Crop Development Centre, University of Saskatchewan for distribution to select seed growers through the Variety Release Committee of the Saskatchewan Pulse Growers. CDC Canary has good lodging resistance, medium-sized round seeds, early maturity, and g...

CDC Athabasca, a yellow cotyledon field pea (Pisum sativum L.) cultivar, was released in 2016 by the Crop Development Centre, University of Saskatchewan for distribution to select seed growers through the Variety Release Committee of the Saskatchewan Pulse Growers. CDC Athabasca has good lodging resistance, large-sized round seeds, and good yieldin...

CDC Forest, a green cotyledon field pea (Pisum sativum L.) cultivar, was released in 2017 by the Crop Development Centre, University of Saskatchewan for distribution to select seed growers through the Variety Release Committee of the Saskatchewan Pulse Growers. CDC Forest has good lodging resistance, medium-sized round seeds, and good yielding abil...

Crop wild relatives are a reservoir of phenotypic variation not present in the germplasm of culti- vated species and thus have great potential for crop improvement. However, issues of genetic compatibility often interfere with effective utili- zation of crop wild relative taxa. Among chick- pea (Cicer arietinum L.) crop wild relatives, Cicer echino...

Field pea (Pisum sativum) is an important pulse crop globally for human consumption and livestock feed. A panel of 92 diverse pea cultivars was evaluated across nine environments and genotyped using 1536 single nucleotide polymorphisms (SNPs) arranged in a GoldenGate array. Population structure analysis revealed three subpopulations roughly consist...

In climates that experience short growing seasons due to drought, heat or end-of-season frost, early flowering is a highly desirable trait for chickpeas. In this study, we mapped, sequenced and characterized Efl1, an ortholog of Arabidopsis ELF3 that confers early flowering in chickpea. In a RIL population derived from a cross between CDC Frontier...

Iron (Fe) deficiency is a major human health concern in areas of the world in which diets are often Fe deficient. In the current study, we aimed to identify appropriate methods and optimal dosage for Fe fortification of lentil (Lens culinaris Medik.) dal with FeSO4·7H2O (ferrous sulphate hepta-hydrate), NaFeEDTA (ethylenediaminetetraacetic acid iro...

Ascochyta blight is one of the major diseases of chickpea worldwide. The genetic resistance to ascochyta blight in chickpea is complex and governed by multiple QTLs. However, the molecular mechanism of quantitative disease resistance to ascochyta blight and the genes underlying these QTLs are still unknown. Most often disease resistance is determin...

Ascochyta blight (AB) is an important disease of pea which can cause severe grain yield loss under wet conditions. In our previous study, we identified two quantitative trait loci (QTLs) abIII-1 and abI-IV-2 for AB resistance and these QTLs were consistent across locations and/or years in an inter-specific pea population (PR-19) developed from a cr...

Field experiments were conducted at three locations with Zn deficient soils in southern Ethiopia during 2012 and 2013 cropping seasons to evaluate the effects of Zn fertilization strategies and varietal differences on Zn content and plant performance of chickpea (Cicer arietinum L.). Factorial combinations of three Zn fertilization strategies and f...