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Bruno Daniel Frans Pot

Bruno Daniel Frans Pot
Yakult Europe BV · Science

PhD

About

255
Publications
94,434
Reads
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24,098
Citations
Introduction
Reseach interest are probiotics, from characterisation, mechanisms of action to regulation. Strongly promoting the collaboration between academic and industry research. Involved in research organisations as ISAPP, IPA, PRI, LABIP, ILSI... to promote honnest and science-based communication to consumers and non-specilaised health care professionals.
Additional affiliations
November 2016 - present
Yakult Europe
Position
  • Managing Director
May 2001 - October 2016
Institut Pasteur de Lille
Position
  • Directeur de Recherche
Description
  • Main topic is probiotic research.
May 2001 - present
Vrije Universiteit Brussel
Position
  • Vrije Universteit Brussels
Description
  • Courses on Food Microbiology, Ecology and Hygiene

Publications

Publications (255)
Article
Full-text available
The gut and brain link via various metabolic and signalling pathways, each with the potential to influence mental, brain and cognitive health. Over the past decade, the involvement of the gut microbiota in gut–brain communication has become the focus of increased scientific interest, establishing the microbiota–gut–brain axis as a field of research...
Chapter
As the body of knowledge on the interaction between the microbiome and its host expands, the industry is taking this opportunity to develop new approaches to improve overall and specific health management. An increasing number of stakeholders are now involved in the development of products containing living microorganisms, aimed at positively influ...
Preprint
Full-text available
Live biotherapeutic products constitute an emerging therapeutic approach to prevent or treat inflammatory bowel diseases. Lactobacillus acidophilus is a constituent of the human microbiota with probiotic potential, that are illustrated by direct and indirect antimicrobial activity against several pathogens and improvement of intestinal inflammation...
Article
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Traditional probiotics are increasingly being used in a medical context. The use of these products as drugs is considerably different from the traditional use as food or food supplements, as, obviously, the target population is different (diseased versus healthy or at risk population). Besides the target population, also the regulatory context is d...
Article
Full-text available
Since alterations of the gut microbiota have been shown to play a major role in obesity, probiotics have attracted attention. Our aim was to identify probiotic candidates for the management of obesity using a combination of in vitro and in vivo approaches. We evaluated in vitro the ability of 23 strains to limit lipid accumulation in adipocytes and...
Article
Full-text available
Prevention and treatment: When living microbes become medicines Regulatory frameworks must be stretched to include microbe-based medicines such as “live biotherapeutic products” (LBPs). Some LBPs are similar by nature to probiotics, health-enhancing living microbes, except that they are meant to treat or prevent disease in sick or vulnerable indivi...
Article
Full-text available
Still relevant after 19 years, the FAO/WHO definition of probiotics can be translated into four simple and pragmatic criteria allowing one to conclude if specific strains of microorganisms qualify as a probiotic for use in foods and dietary supplements. Probiotic strains must be (i) sufficiently characterized; (ii) safe for the intended use; (iii)...
Article
Full-text available
Recent developments in the understanding of the relationship between the microbiota and its host have provided evidence regarding the therapeutic potential of selected microorganisms to prevent or treat disease. According to Directive 2001/83/EC, in the European Union (EU), any product intended to prevent or treat disease is defined as a medicinal...
Article
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The ninth International Yakult Symposium was held in Ghent, Belgium in April 2018. Keynote lectures were from Professor Wijmenga on using biobanks to understand the relationship between the gut microbiota and health; and Professor Hill on phage–probiotic interactions. Session one included talks from Professor Plӧsch on epigenetic programming by nut...
Article
Full-text available
The genus Lactobacillus comprises 261 species (at March 2020) that are extremely diverse at phenotypic, ecological and genotypic levels. This study evaluated the taxonomy of Lactobacillaceae and Leuconostocaceae on the basis of whole genome sequences. Parameters that were evaluated included core genome phylogeny, (conserved) pairwise average amino...
Article
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Crohn’s disease is linked to a decreased diversity in gut microbiota composition as a potential consequence of an impaired anti-microbial response and an altered polarization of T helper cells. Here, we evaluated the immunomodulatory properties of two potential probiotic strains, namely a Bifidobacterium animalis spp. lactis Bl 5764 and a Lactobaci...
Article
Full-text available
Streptococcus thermophilus is a major starter for the dairy industry with great economic importance. In this study we analyzed 23 fully sequenced genomes of S. thermophilus to highlight novel aspects of the evolution, biology and technological properties of this species. Pan/core genome analysis revealed that the species has an important number of...
Article
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Background/aims: Deregulation of the complex interaction among host genetics, gut microbiota and environmental factors on one hand and aberrant immune responses on the other hand, are known to be associated with the development of inflammatory bowel disease. Recent studies provided strong evidence that autophagy plays a key role in the etiology of...
Article
Background Taxonomy and nomenclature are important aspects of biological science, as they allow unambiguous communication about all living species. In contrast to higher life forms, microbes have no comparable sexual reproduction, upon which bacterial speciation can be based. Therefore, alternative criteria have to be used, which differ depending o...
Article
Full-text available
Lactobacillus delbrueckii subsp. lactis is employed in the production of various types of cheese. Here, we report the complete genome sequence of L. lactis ACA-DC 178 isolated from Greek Kasseri cheese. The chromosome of ACA-DC 178 contains 2,050,316 bp with a GC content of 49.6%. A total of 2,112 genes were identified in the genome sequence includ...
Article
Background: A potential positive effect of probiotics in cystic fibrosis (CF) on fecal calprotectin (FCP), pulmonary exacerbations and weight has been described in small controlled trials. Methods: A double-blind multicenter cross-over study (2 × 4 m) was performed looking at abdominal pain, nutritional status, pulmonary function, pulmonary exac...
Article
Full-text available
Lactobacillus acidipiscis belongs to the Lactobacillus salivarius clade and it is found in a variety of fermented foods. Strain ACA-DC 1533 was isolated from traditional Greek Kopanisti cheese and among the available L. acidipiscis genomes it is the only one with a fully sequenced chromosome. L. acidipiscis strains exhibited a high degree of conser...
Article
Alteration of the gut microbiota together with an increase in intestinal permeability and persistent inflammatory responses are thought to be critical factors in the pathogenesis of inflammatory bowel disease (IBD). Taking benefit of these knowledges, a proper strategy to address the selection of efficient probiotics is needed more than ever. The p...
Article
Full-text available
Gut microbiota play a key role in the development of metabolic disorders. Defining and correlating structural shifts in gut microbial assemblages with conditions related to metabolic syndrome have, however, been proven difficult. Results from 16S genomic DNA and 16S ribosomal RNA analyses of fecal samples may differ widely, leading to controversial...
Article
Full-text available
As normal inhabitants of diverse ecosystems, including the human gastrointestinal tract, the enterococci, and especially the two species Enterococcus faecalis and Enterococcus faecium, can be considered ubiquitous with regard to our natural environment. E. faecium has gained special importance thanks to beneficial strains marketed as probiotics, an...
Article
Full-text available
Alterations in the gut microbiota composition play a key role in the development of chronic diseases such as inflammatory bowel disease (IBD). The potential use of probiotics therefore gained attention, although outcomes were sometimes conflicting and results largely strain-dependent. The present study aimed to identify new probiotic strains that h...
Article
Full-text available
Probiotics such as L. plantarum WCFS1 can modulate immune responses in healthy subjects but how this occurs is still largely unknown. Immune-sampling in the Peyer Patches has been suggested to be one of the mechanisms. Here we studied the systemic and intestinal immune effects in combination with a trafficking study through the intestine of a well-...
Chapter
The ban of antibiotics at subtherapeutic levels as growth promoters in animals, along with the emerging challenges and prospects deriving from the use of probiotics and prebiotics in humans, opened the era of similar applications in the field of animal nutrition. Probiotics and prebiotics as novel additives in animal feeding aiming at host-protecti...
Article
Full-text available
Streptococcus thermophilus ACA-DC 2 is a newly sequenced strain isolated from traditional Greek yogurt. Among the 14 fully sequenced strains of S. thermophilus currently deposited in the NCBI database, the ACA-DC 2 strain has the smallest chromosome, containing 1,731,838 bp. The annotation of its genome revealed the presence of 1,850 genes, includi...
Article
Comprehension of underlying mechanisms of probiotic action will support rationale selection of probiotic strains and targeted clinical study design with a higher likelihood of success. This will consequently contribute to better substantiation of health claims. Here, we aim to provide a perspective from a microbiology point of view that such compre...
Article
Lactobacillus delbrueckii subsp. bulgaricus is widely used in the production of yogurt and cheese. In this study, we present the complete genome sequence of L. delbrueckii subsp. bulgaricus ACA-DC 87 isolated from traditional Greek yogurt. Whole-genome analysis may reveal desirable technological traits of the strain for dairy fermentations.
Article
Full-text available
Lactobacillus zymae is a Gram-positive lactic acid bacterium belonging to the Lactobacillus brevis clade. Here, we report the first complete genome sequence of L. zymae ACA-DC 3411, which was isolated from traditional Greek wheat sourdough. Whole-genome analysis may reveal adaptive traits of strain ACA-DC 3411 in the sourdough ecosystem.
Chapter
The analysis of matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) spectra has been integrated in the BioNumerics software. This chapter discusses the different algorithms available for processing the raw spectra. It discusses how to handle technical and biological replicates and illustrates the statistical...
Article
Full-text available
: Probiotics have been proposed for a number of indications ranging from the hypothetical long term immunomodulatory effects to proven benefits in the management of different clinical conditions.An increasing number of commercial products containing probiotics are available. In those products, irrespective if it is food, food supplement, medical fo...
Article
Full-text available
Gut microbiota dysbiosis plays a central role in the development and perpetuation of chronic inflammation in inflammatory bowel disease (IBD) and therefore is key target for interventions with high quality and functional probiotics. The local production of stable probiotic formulations at limited cost is considered an advantage as it reduces transp...
Article
Full-text available
In this study, we present the first complete genome sequence of Lac-tobacillus rennini ACA-DC 565, a strain isolated from a traditional Greek overripened Kopanisti cheese called Mana. Although the species has been associated with cheese spoilage, the strain ACA-DC 565 may contribute to the intense organoleptic characteristics of Mana cheese.
Article
Full-text available
Lactobacillus acidipiscis is a Gram-positive lactic acid bacterium belonging to the Lactobacillus salivarius clade. Here, we present the first complete genome sequence of L. acidipiscis isolated from traditional Greek Kopanisti cheese. Strain ACA-DC 1533 may play a key role in the strong organoleptic characteristics of Kopanisti cheese.
Article
Listeria monocytogenes (Lm) is a major human foodborne pathogen. Numerous Lm outbreaks have been reported worldwide and associated with a high case fatality rate, reinforcing the need for strongly coordinated surveillance and outbreak control. We developed a universally applicable genome-wide strain genotyping approach and investigated the populati...
Article
Full-text available
Listeria monocytogenes (Lm) is a major human foodborne pathogen. Numerous Lm outbreaks have been reported worldwide and associated with a high case fatality rate, reinforcing the need for strongly coordinated surveillance and outbreak control. We developed a universally applicable genome-wide strain genotyping approach and investigated the populati...
Article
Today the importance of the so-called health-promoting bacteria or probiotics has been well established. Many of these probiotics are being delivered through fermented food products, of which dairy foods are among the most important carriers. The purpose of this report is to review some of the newer applications of probiotics that are situated far...
Article
Full-text available
Multidrug resistant Acinetobacter baumannii and its closely related species A. pittii and A. nosocomialis, all members of the Acinetobacter calcoaceticus-baumannii (Acb) complex, are a major cause of hospital acquired infection. In the burn wound center of the Queen Astrid military hospital in Brussels, 48 patients were colonized or infected with A...
Article
Full-text available
Here we analyze the first complete genome sequence of Pyrococcus chitonophagus. The archaeon was previously suggested to belong to the Thermococcus rather than the Pyrococcus genus. Whole genome phylogeny as well as whole proteome comparisons using all available complete genomes in Thermococcales clearly showed that the species belongs to the Pyroc...
Article
An accurate amplified fragment length polymorphism (AFLP) method, including three primer sets for the selective amplification step, was developed to display the phylogenetic position of Photobacterium isolates collected from salmon products. This method was efficient for discriminating the three species Photobacterium phosphoreum, Photobacterium il...
Article
Alterations in gut microbiota composition and diversity were suggested to play a role in the development of obesity, a chronic subclinical inflammatory condition. We here evaluated the impact of oral consumption of a monostrain or multistrain probiotic preparation in high-fat diet-induced obese mice. We observed a strain-specific effect and reporte...
Article
Full-text available
Cancer cachexia is a multifactorial syndrome that includes muscle wasting and inflammation. As gut microbes influence host immunity and metabolism, we investigated the role of the gut microbiota in the therapeutic management of cancer and associated cachexia. A community-wide analysis of the caecal microbiome in two mouse models of cancer cachexia...
Conference Paper
Full-text available
Campylobacter infections are still the most common causes of diarrheal illness in the developed world. A key challenge to applying the widely available next generation sequencing technology to outbreak detection is the need to rapidly extract the relevant information from large datasets covering the whole genome sequences (WGS). We present a highly...
Chapter
Le.vi.spi.ril' lum. L. adj. laevus left; M.L. dim. neut. n. spirillum small spiral; Levispirillum small spiral-shaped bacteria with left-turning helix. Proteobacteria / Alphaproteobacteria / Rhodospirillales / Rhodospirillaceae / Levispirillum Gram-negative, rigid, helical cells 0.4?0.7 ?m in diameter and 2?22 ?m in length. Helix is left-handed, de...
Chapter
Aq.ua.spi.ril' lum. L. aqua water; Gr. n. spira a spiral; N.L. dim. neut. n. spirillum a small spiral; Aquaspirillum a small water spiral. Proteobacteria / Betaproteobacteria / Neisseriales / Neisseriaceae / Aquaspirillum Rigid, helical cells, 0.2–1.4 µm in diameter; however, one species is vibrioid and one species contains straight-to-curved rods....
Chapter
Spor.o.spi.ril' lum. Gr. n. sporos a seed (spore); Gr. n. spira a spiral; M.L. dim. n. spirillum a small spiral; M.L. neut. n. Sporospirillum a small spore (-forming) spiral. Proteobacteria / Alphaproteobacteria / Rhodospirillales / Rhodospirillaceae / incertae Sedis XII. “Sporospirillum” Rigid, helical bacteria of enormous size, 1.8–4.8 μm in diam...
Chapter
O.ce.an.o.spi.ril' lum. M.L. n. oceanus ocean; Gr. n. spira a spiral; M.L. dim. neut. n. spirillum spirillum a small spiral; Oceanospirillum a small spiral (organism) from the ocean (seawater). Proteobacteria / Gammaproteobacteria / Oceanospirillales / Oceanospirillaceae / Oceanospirillum Rigid, helical cells 0.4–1.2 μm in diameter. Motile by bipol...
Article
Full-text available
Enterococcus gallinarum and E. casseliflavus are difficult to differentiate from other enterococci, particularly E. faecium. The former two species were found to produce acid from methyl-a-D-glucopyranoside in phenol red broth, while E. faecalisstrains and strains of the E. faecium species group, including E. faecium, E. durans,E. hirae, and E. mun...
Article
The potential benefits of probiotics on human health are the subject of an increasing number of publications. Studies show that their use can potentially have a very positive effect on public health care costs, notably with regard to the prevention of diarrhoea and chest infections. However, all health claims have, to date, been refused for foods c...
Article
Full-text available
Lactic acid bacteria are found in the gastrointestinal tract of mammals and have received tremendous attention due to their health-promoting properties. We report the development of two dual color luciferase-producing Lactobacillus plantarum and Lactococcus lactis for noninvasive simultaneous tracking in the mouse gastrointestinal tract. We previou...
Article
The species Bifidobacterium longum is currently divided into three subspecies, B. longum subsp. longum, B. longum subsp. infantis and B. longum subsp. suis. This classification was based on an assessment of accumulated information on the species' phenotypic and genotypic features. The three subspecies of B. longum were investigated using genotypic...
Article
Full-text available
The beneficial effects of carbohydrate-derived fibers are mainly attributed to modulation of the microbiota, increased colonic fermentation, and the production of short-chain fatty acids. We studied the direct immune responses to alimentary fibers in in vitro and in vivo models. Firstly, we evaluated the immunomodulation induced by nine different t...
Article
Standardization in comparison and interpretation of profiles is an integral part of the analysis of gels from pulsed-field gel electrophoresis. Using Listeria monocytogenes as an example, this chapter outlines the analytical process using the software BioNumerics.
Article
Lactic acid bacteria (LAB), yeasts and molds are employed as starters for the production of fermented foods. Adaptation of starter microorganisms in diverse food niches was accompanied by gene loss and gene gain events. Even though these events may differ between prokaryotic and eukaryotic starters, they reveal evolution toward high fermentation ca...
Article
Full-text available
Over the past decades the food industry has been revolutionized toward the production of functional foods due to an increasing awareness of the consumers on the positive role of food in wellbeing and health. By definition probiotic foods must contain live microorganisms in adequate amounts so as to be beneficial for the consumer's health. There are...
Article
Full-text available
Streptococcus macedonicus is an intriguing streptococcal species whose most frequent source of isolation is fermented foods similarly to Streptococcus thermophilus. However, S. macedonicus is closely related to commensal opportunistic pathogens of the Streptococcus bovis/Streptococcus equinus complex. We analyzed the pSMA198 plasmid isolated from t...