Bruno Miranda

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Verified

Bruno verified their affiliation via an institutional email.

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• Ph. D.
• Researcher (Fixed Term) at Italian National Research Council

A 3D plasmonic sensing platform that combines the properties of citrate gold nanoparticles (AuNPs) and poly-(ethylene glycol) diacrylate (PEGDA) hydrogels is proposed as a nanocomposite hybrid material for biosensing applications, whose optical properties and sensitivity can be tuned by varying the particle mean diameter as also predicted by the Mi...

Over the last 30 years, optical biosensors based on nanostructured materials have obtained increasing interest since they allow the screening of a wide variety of biomolecules with high specificity, low limits of detection, and great sensitivity. Among them, flexible optical platforms have the advantage of adapting to non-planar surfaces, suitable...

A hybrid plasmonic transducer made of a Poly‐(ethylene glycol) diacrylate (PEGDA) hydrogel and citrate gold nanoparticles detects the biotin‐streptavidin interaction at picomolar (× 10⁻¹² m ) concentrations. The all‐solution fabrication strategy, herein proposed, is large‐scale, easily tunable, and low‐cost; nevertheless, this innovative device is...

Sulfur dioxide (SO2) and sulfites are well-known additives in winemaking due to their preservative properties. Although they can prevent oxidation and inhibit microbial growth, they pose health risks and require limitations on their use. Consequently, the total level of SO2 is regulated and several quantification strategies have been proposed. The...

The integration of advanced materials and photonic nanostructures can lead to enhanced biodetection capabilities, crucial in clinical scenarios and point‐of‐care diagnostics, where simplified strategies are essential. Herein, a molecularly imprinted polymer (MIP) photonic nanostructure is demonstrated, which selectively binding to transforming grow...

IgM is the major circulating Ig isotype in teleost fish, showing in Antarctic fish unique features such as an extraordinary long hinge region, which plays a crucial role in antibody structure and function. In this work, we describe the replacement of the hinge region of a murine monoclonal antibody (mAb) with the peculiar hinge from Antarctic fish...

The synthesis of metallic plasmonic nanoparticles (NPs) faces challenges in stability and reproducibility, especially with silver. Here, we present a protocol for tunable synthesis of spherical silver NPs (AgNPs) with stable optical properties. We describe steps for preparing solutions, morphological characterization of AgNPs by transmission electr...

The combination of plasmonic nanoparticles and hydrogels results in nanocomposite materials with unprecedented properties that give rise to powerful platforms for optical biosensing. Herein, we propose a physicochemical characterization of plasmonic hydrogel nanocomposites made of polyethylene glycol diacrylate (PEGDA) hydrogels with increasing mol...

Antimicrobial activity of many AMPs can be improved by lysine-to-arginine substitution due to a more favourable interaction of arginine guanidinium moiety with bacterial membranes. In a previous work, the structural and functional characterization of an amphipathic antimicrobial peptide named RiLK1, including lysine and arginine as the positively c...

IgM is the major circulating Ig isotype in teleost fish, showing in Antarctic fish unique features such as an extraordinary long hinge region, which plays a crucial role in antibody structure and function. In this work, we describe the replacement of the hinge region of a murine monoclonal antibody (mAb) with the peculiar hinge from Antarctic fish...

Herein, a novel completely green biosensor was designed exploiting both the biological and instrumental components made of eco-friendly materials for the detection of herbicides encapsulated into biodegradable nanoparticles for a sustainable agriculture. Similar nanocarriers, indeed, can deliver herbicides to the correct location, reducing the amou...

Interstitial fluid (ISF) extraction and analysis are challenges that can be tackled by Hollow MicroNeedles (HMNs) technology, overcoming most of the difficulties associated with in situ detection. Herein, a plasmonic transducer, composed of gold nanoparticles embedded in poly(ethylene glycol) diacrylate (PEGDA) hydrogels, is integrated in the inner...

Plasmonic Transducers In article number 2101425, Bruno Miranda, Carlo Forestiere, and co‐workers report on hydrogel‐based, high‐sensitivity, hybrid plasmonic transducers for monitoring biomolecular interactions. The hydrogel network physically stabilizes gold nanoparticles, which act as biochemical optical transducers both in spectroscopy and fluor...

Current advances in the fabrication of smart nanomaterials and nanostructured surfaces find wide usage in the biomedical field. In this context, nanosensors based on localized surface plasmon resonance exhibit unprecedented optical features that can be exploited to reduce the costs, analytic times, and need for expensive lab equipment. Moreover, th...

Inorganic diatomite nanoparticles (DNPs) have gained increasing interest as drug delivery systems due to their porous structure, long half-life, thermal and chemical stability. Gold nanoparticles (AuNPs) provide DNPs with intriguing optical features that can be engineered and optimized for sensing and drug delivery applications. In this work, we co...

The electromagnetic scattering from a network of high-permittivity dielectric thin wires with the network’s size smaller than or almost equal to the operating wavelength is investigated. A simple lumped-element theory for the polarization current intensities induced in the wires is developed. The network elements are capacitances and inductances be...

Localized surface plasmon resonance (LSPR) and metal-enhanced-fluorescence (MEF)-based optical biosensors exhibit unique properties compared to other sensing devices that can be exploited for the design point-of-care (POC) diagnostic tools [1]. Plasmonic devices exploit the capability of noble-metal nanoparticles of absorbing light at a well-define...

An easy and fast fabrication strategy to obtain Photochromic Films (PFs) for naked-eye detection of oxygen is presented. These PFs are based on the photoreductive activity of TiO2 nanoparticles combined with the redox-driven color switching property of methylene blue, embedded in a photocurable and tunable air-permeable polyethylene glycol diacryla...

The electromagnetic scattering from interconnections of high-permittivity dielectric thin wires with sizes smaller than (or almost equal to) the operating wavelength is investigated. A simple lumped element model for the polarization current intensities induced in the wires is proposed. The circuit elements are capacitances and inductances between...

Microneedles (MNs) are an emerging technology in pharmaceutics and biomedicine, and are ready to be commercialized in the world market. However, solid microneedles only allow small doses and time-limited administration rates. Moreover, some well-known and already approved drugs need to be re-formulated when supplied by MNs. Instead, hollow micronee...

Proteins are a class of intriguing biomolecules since they are involved in major biological and vital processes. As a result of their variety and complexity, proteins are widely used in biotechnological applications and they are the most used macromolecules in the biosensing field. Proteins orientation on the transducer's substrate is crucial for b...

Biosensing systems based on microneedles can overcome the stratum corneum of the skin, i. e. the outer natural barrier of the human body, without any pain and detect the target analytes directly in the interstitial fluid. Moreover, microneedle-based devices (MNDs) can combine diagnostic sensing and therapeutic administration of drugs in one single...

The recent advances in nanofabrication processes offer encouraging opportunities for designing highly sensitive detection tools. One example is metal-enhanced fluorescence (MEF)-based biosensors: by effectively coupling the metal nanostructures with the fluorescent dye used for the detection of the target molecule, MEF-based sensors exhibit higher...

High-index objects constitute nanoscale optical conduits for the displacement current density field because its normal component to the object walls vanishes. In this paper, we demonstrate that by squeezing the section of a high-index ring resonator, the conservation of the flux of displacement current density naturally imposes the enhancement and...