Bruce A. Kimball

• Faculty Member at Monell Chemical Senses Center

Infections have been shown to alter body odor. Because immune activation accompanies both infection and immunization, we tested the hypothesis that classical immunization might similarly result in alteration of body odors detectable by trained biosensor mice. Using a Y-maze, we trained biosensor mice to distinguish between urine odors from rabies-v...

Changes in body odor are known to be a consequence of many diseases. Much of the published work on disease-related and body odor changes has involved parasites and certain cancers. Much less studied have been viral diseases, possibly due to an absence of good animal model systems. Here we studied possible alteration of fecal odors in animals infect...

The vomiting (emetic) reflex is documented in numerous mammalian species, including primates and carnivores, yet laboratory rats and mice appear to lack this response. It is unclear whether these rodents do not vomit because of anatomical constraints (e.g., a relatively long abdominal esophagus) or lack of key neural circuits. Moreover, it is unkno...

Foraging behavior is an expression of learning, context, and experience arising from integration of sensory information obtained during feeding with postingestive consequences of food ingestion. Although it has been well established that gustatory and olfactory systems of the mouth and nose provide sensory information to the consumer (in the form o...

Chronic wasting disease (CWD) has become a major concern among those involved in managing wild and captive cervid populations. CWD is a fatal, highly transmissible spongiform encephalopathy caused by an abnormally folded protein, called a prion. Prions are present in a number of tissues, including feces and urine in CWD infected animals, suggesting...

Individuals may have a different body odor, when they are sick compared to healthy. In the non-human animal literature, olfactory cues have been shown to predict avoidance of sick individuals. We tested whether the mere experimental activation of the innate immune system in healthy human individuals can make an individuals’ body odor be perceived a...

Wildlife disease surveillance and monitoring poses unique challenges when assessing rates of population vaccination, immunity, or infection prevalence. Non-invasively detected biomarkers can help reduce risk to both animal and field personnel during wildlife disease management activities. In this study, we investigated the utility of fecal microbio...

Nicotine is an addictive drug whose popularity has recently increased, particularly among adolescents, because of the availability of electronic nicotine devices (i.e., “vaping”) and nicotine e-liquids containing additives with rich chemosensory properties. Some efforts to understand the role of these additives in nicotine reward suggest that they...

Nicotine is an addictive drug whose popularity has recently increased, particularly among adolescents, due to the availability of electronic nicotine devices ( i . e ., “vaping”) and nicotine e-liquids containing additives with rich chemosensory properties. Some efforts to understand the role of these additives in nicotine reward suggest that they...

Background: Sinonasal inverted papilloma (SNIP) is a benign neoplasm with aggressive features, including a high recurrence rate and a propensity for malignant transformation. Accurate diagnosis with complete resection and the need for close long-term surveillance is widely accepted as standard management. In this study, we investigate whether SNIP...

Recent avian influenza infection outbreaks have resulted in global biosecurity and economic concerns. Mallards are asymptomatic for the disease and can potentially spread AI along migratory bird flyways. In a previous study, trained mice correctly discriminated the health status of individual ducks on the basis of fecal odors when feces from post-i...

Dead neonatal mice are currently used as bait for delivery of toxin to invasive brown treesnakes (Boiga irregularis) on Guam; once deployed in the field the mice are highly attractive to the snakes but only for about four days. An artificial bait containing a mixture of fats mimicking those in skin of the mice is also highly attractive to the snake...

Alterations of the volatile metabolome (the collection of volatiles present in secretions and other emanations) that occur in response to inflammation can be detected by conspecifics and chemometric analyses. Using a model system where mouse urinary metabolites are altered by treatment with lipopolysaccharide (LPS; found in the outer cell membrane...

The rise of e-cigarette popularity has sparked interest in the role of palatable flavors on nicotine use. Despite growing evidence that sweet flavorants enhance nicotine reward, their influence on nicotine consumption has not been studied extensively. In addition, the impact that flavored nicotine use in adolescence could have on nicotine reward an...

Outbreaks of avian influenza virus (AIV) infection included the spread of highly pathogenic AIV in commercial poultry and backyard flocks in the spring of 2015. This resulted in estimated losses of more than $8.5 million from federal government expenditures, $1.6 billion from direct losses to produces arising from destroyed turkey and chicken egg p...

The rise of e-cigarette popularity has sparked interest in the role of palatable flavors on nicotine use. Despite growing evidence that sweet flavorants enhance nicotine reward, their influence on nicotine consumption has not been studied extensively. In addition, the impact that flavored nicotine use in adolescence could have on nicotine reward an...

Animals detect sick conspecifics by way of body odor that enables the receiver to avoid potential infectious transmission. Human observational studies also indicate that different types of disease are associated with more or less aversive smells. In addition, body odors from otherwise healthy human individuals smell more aversive as a function of e...

For humans, like other social animals, behaviour acts as a first line of defence against pathogens. A key component is the ability to detect subtle perceptual cues of sick conspecifics. The present study assessed the effects of endotoxin-induced olfactory and visual sickness cues on liking, as well as potential involved mechanisms. Seventy-seven pa...

Rabies is a zoonotic disease caused by infection with rabies virus, which circulates naturally in several wild carnivore and bat reservoirs in the United States (US). The most important reservoir in the US from an animal and public health perspective is the raccoon (Procyon lotor). To prevent the westward expansion of a significant raccoon rabies e...

Prairie voles (Microtus ochrogaster Wagner) cause extensive damage in agricultural, suburban, and urban environments. Control of these animals has historically relied on the use of anticoagulant rodenticides and zinc phosphide. However, shyness to zinc phosphide baits has reduced its efficacy. The aim of this study was to evaluate the factors invol...

Abstract Body odors change with health status and the odors of sick animals can induce avoidance behaviors in healthy conspecifics. Exposure to sickness odors might also alter the physiology of healthy conspecifics and modify the odors they produce. We hypothesized that exposure to odors of sick (but non-infectious) animals would alter the odors of...

Contagious disease is a major threat to survival, and the cost of relying on the immune system to defeat pathogens is high; therefore, behavioral avoidance of contagious individuals is arguably an adaptive strategy. Animal findings demonstrate the ability to detect and avoid sick individuals by the aid of olfactory cues, and a recent study indicate...

Broadening our knowledge of olfactory communication in strictly monogamous systems can inform our understanding of how chemosignals may facilitate social and reproductive behavior between the sexes. Compared to other social and mating systems, relatively little is known about olfactory communication in strictly monogamous non-human primates. Furthe...

Pheromones are useful tools for the management of invasive invertebrates, but have proven less successful in field applications for invasive vertebrates. The brown treesnake, Boiga irregularis, is an invasive predator that has fundamentally altered the ecology of Guam. The development of control tools to manage Boiga remains ongoing. Skin-based, li...

Several studies demonstrate that inflammation affects body odor. Volatile signals associated with inflammation induced by pyrogens like LPS are detectable both by conspecifics and chemical analyses. However, little is known about the mechanisms which translate detection of a foreign molecule or pathogen into a unique body odor, or even how unique t...

Chemical signals arising from body secretions and excretions communicate information about health status as have been reported in a range of animal models of disease. A potential common pathway for diseases to alter chemical signals is via activation of immune function-which is known to be intimately involved in modulation of chemical signals in se...

The brown treesnake (Boiga irregularis) was accidentally introduced to Guam in the 1940s from the Admiralty Islands. A native of Australia, Papua New Guinea, and the Solomon Islands, the brown treesnake (BTS) continues to threaten the economy and ecology of Guam and is currently the subject of a cooperative program to control snake populations on t...

In the present study, we tested whether the volatile metabolome was altered by mutations of the Alzheimer’s disease (AD)-implicated amyloid precursor protein gene (APP) and comprehensively examined urinary volatiles that may potentially serve as candidate biomarkers of AD. Establishing additional biomarkers in screening populations for AD will prov...

Background: Boiga irregularis is a widespread invasive species on Guam and has led to extirpation of most of the island's native avifauna. There are presently no microsatellite markers for this invasive species, hence we developed highly polymorphic microsatellite markers to allow for robust population genetic studies on Guam. Findings: We isola...

Observational studies have suggested that with time, some diseases result in a characteristic odor emanating from different sources on the body of a sick individual. Evolutionarily, however, it would be more advantageous if the innate immune response were detectable by healthy individuals as a first line of defense against infection by various path...

Observational studies have suggested that with time, some diseases result in a characteristic odor emanating from different sources on the body of a sick individual. Evolutionarily, however, it would be more advantageous if the innate immune response were detectable by healthy individuals as a first line of defense against infection by various path...

We examined the repellency of a commercially available animal repellent to determine the efficacy of its application to objects that are attractive to coyotes (Canis latrans). Specifically, we aimed to both prevent chewing behavior by coyotes on a nylon-like strapping material, which is used to construct barrier-arresting systems on military airstr...

[This corrects the article on p. e60537 in vol. 8.].

Predators are capable of causing damage to domestic livestock through-out North America. Lethal responses for managing livestock depredations may include the use of sodium cyanide in M-44 devices. Currently, several states have banned the use of M-44s and several other states are forecast to ban these devices. Therefore, additional tools are being...

To determine how black-tailed deer Odocoileus hemionus columbianus respond to phytochemical cues while browsing in heterogeneous phytochemical environments, we offered captive and free-range deer cloned rooted cuttings and seedlings of western redcedar Thuja plicata selected for varying monoterpene content. Black-tailed deer were thus allowed to br...

Certain phenotypic traits of plants vary with latitude of origin. To understand if tannin concentration varies among populations of tamarisk (Tamarix spp.) according to a latitudinal gradient, an analytical method was adapted from an enological tannin assay. The tannin content (wet basis) of tamarisk foliage collected from 160 plants grown in a com...

A multiyear study was conducted to determine if soil amendment combined with topical application of elemental sulfur could be employed to reduce deer browse damage to four conifer species. Fertilizer and sulfur were applied to conifer seedlings at seven sites near Corvallis, OR. Growth and browse damage data were collected for all seedlings over a...

Rejection of bitter substances is common in many species and may function to protect an animal from ingestion of bitter-tasting toxins. Since many plants are bitter, it has been proposed that high tolerance for bitterness would be adaptive for herbivores. Earlier studies conducted on herbivorous guinea pigs (Cavia porcellus) have been used to suppo...

Mammalian herbivores are responsible for significant damage that may total billions of dollars to a variety of agricultural products, including crops, landscape ornamentals, and timber. The challenge of the resource manager is to minimize the conflict between herbivore and human activity while recognizing that consuming plant material is the herbiv...

Laboratory rats and mice are known to lack a vomiting response and the dimensions of the abdominal esophagus might be an important constraint (Andrews, 1995, Physiol. Zool.). However, a broad evaluation of Rodentia is lacking. Here we determined the behavioral responses and esophageal and diaphragm anatomy from 4 of the 5 Suborders.Weused prototypi...

Learned avoidance of a specific food resulting from negative postingestive consequences is known as taste aversion learning (TAL) or flavor aversion learning. Although TAL causes avoidance behavior, taste aversion is a unique category of avoidance. Taste aversions arise specifically from exposure to stimuli that produce nausea and a qualitative shi...

This presentation reviews browse resistant field trials that have been conducted to measure deer responses to experimental manipulations of western redcedar foliar chemistry. Selective breeding was employed to produce planting stock of seedlings and cuttings with varying levels of monoterpenes. These studies examined black-tailed deer preferences a...

Four repellents representing different modes of action (neophobia, irritation, conditioned aversion, and flavor modification) were tested with captive white-tailed deer in a series of two-choice tests. Two diets differing significantly in energy content were employed in choice tests so that incentive to consume repellent-treated diets varied accord...

Several protein sources were tested as deer repellents with captive deer. Black-tailed deer were offered Western redcedar seedlings treated with corn gluten meal, feather meal, poultry blood, hydrolyzed casein, or a control (latex sticker used for treatment proteins). After 37 days, feather meal and hydrolyzed casein provided equivalent protection...

The negative sensory properties of casein hydrolysates (HC) often limit their usage in products intended for human consumption, despite HC being nutritious and having many functional benefits. Recent, but taxonomically limited, evidence suggests that other animals also avoid consuming HC when alternatives exist. We evaluated ingestive responses of...

Individual body weights (g) of rats (top) and guinea pigs (bottom). BW1 was taken as a baseline, following arrival to the facility; BW2 was taken after the Training diets, BW3 was taken after the HC tests, BW4 was taken after the GE tests; BWs taken once per week; note the different y-axis scales. For the rats, all males were heavier than females b...

Individual body weights (g) of mice. Top: Mus, middle: Peromyscus maniculatus, bottom: P. leucopus; species abbreviations are shown on the y-axis label (note different scales). Diets animals had been fed prior to each BW measurement: BW3–4 = Training; BW6–7 = HC, BW9–10 = GE; all others = Maintenance; BWs taken 2–3 d apart. (1.99 MB TIF)

Species diets determined from stomach contents, feces, and direct and indirect observation of feeding in free-ranging, wild populations. (0.04 MB DOC)

Three independent experiments were conducted to evaluate hydrolyzed casein deer repellent to minimize browse damage in reforestation efforts. In the first experiment, western redcedar seedlings were treated with 12% hydrolyzed casein and a latex sticker or one of two commercial deer repellents in the nursery prior to a 45-day cold storage period. T...

To evaluate methods for promoting consumption of tamarisk plants by beavers (Castor canadensis), we determined the feeding responses by captive beavers to diets that contained tannins and sodium chloride (hereafter referred to as tamarisk diet). In two-choice tests, beavers consumed equivalent quantities of tamarisk diet and control diet. Treatment...

Various species of rodents and lagomorphs were used in bioassays to determine the effectiveness of protein hydrolysates (specifically hydrolyzed casein and gelatin) as herbivore repellents. Mixed sex groups of captive rabbits, pocket gophers, voles, and mountain beavers were offered hydrolyzed casein or gelatin test diets in single-choice tests fol...

When casein, a milk protein, is hydrolyzed, it renders human foods that contain it (e.g., hypoallergenic infant formula, cheeses) distasteful to many people. This rejection of hydrolyzed casein (HC)-containing foods has recently been found to also occur in a non-human species (deer, Odocoileus spp.). Identifying other animals that avoid HC would fa...

The relationship between food flavors and postingestive feedback enables mammalian herbivores to procure nutrients and avoid toxins within ever-changing environments. We conducted four experiments with red-winged blackbirds (Agelaius phoeniceus) in captivity to determine the relative roles of color and flavor cues paired with negative postingestive...

Goats were first offered a novel, maple-flavoured diet paired with either lithium chloride (LiCl) or oxalic acid (OA) delivered intraruminally. Aversion to maple diet persisted for 6 days, regardless of toxin. Following a 10-day intermission, aversion to maple diet was reacquired (spontaneous recovery) among subjects previously dosed with LiCl but...

Previously it has been shown that the floral scent of snapdragon flowers consists of a relatively simple mixture of volatile organic compounds (VOCs). These compounds are thought to be involved in the attraction of pollinators; however, little is known about their effect on other organisms, such as neighboring plants. Here, we report that VOCs from...

We have identified hydrolyzed casein as a promising repellent for minimizing damage to forest resources inflicted by browsing ungulates. Eight and twelve percent hydrolyzed casein formulations prepared in water with a latex-based agricultural sticker significantly reduced browse damage by captive black-tailed deer (Odocoileus hemionus columbianus)...

Symbiosis between legume species and rhizobia results in the sequestration of atmospheric nitrogen into ammonium, and the early mechanisms involved in this symbiosis have become a model for plant-microbe interactions and thus highly amenable for agricultural applications. The working model for this interaction states that the symbiosis is the outco...

A mixture of 16.47±0.038 L concentrated capsicum oleoresin with 163.8±3.63 L water and a total of 17.2 L concentrated soybean oil with 177.1 L water was dispensed over five 4.87 m×4.87 m plots via a grid of 128 augured holes (≈15 cm-diameter×≈30 cm deep) in a compacted, sandy loam soil. During the next 9 days, a total of 37 soil samples were obtain...

Hydrolyzed casein (HC) and retail products that contain HC are evaluated as repellents to minimize deer damage to trees and shrubs. Three different experiments demonstrate that HC is an effective deer repellent. Technical-grade HC completely eliminated browse damage to evergreen shrubs (Gaultheria shallon Pursh.) and conifers (Thuja plicata Donn.)...

An analytical method to evaluate propionylpromazine hydrochloride (PPZHCl) in tranquilizer formulations was developed using high-performance liquid chromatography (HPLC). During analysis of aged quality-control samples, a previously unreported chromatographic response was observed at a shorter retention time than PPZHCl. Further investigation of fo...

Multiple experiments were conducted with a mammalian herbivore to determine how experience with plant secondary metabolites (terpenes and tannins) influenced acquisition of new aversions to diets containing these same plant metabolites. Goats (Capra aegagrus hircus) were employed as behavioral models for this study. Twenty-four subjects were assign...

A method is described for quantitative analysis of monoterpenes in western redcedar (Thuja plicata) foliage by gas chromatography with flame ionization detection. Response factors for monoterpenes identified in redcedar are evaluated to determine similarities among monoterpene responses. Evaluation demonstrates that redcedar monoterpenes yield dete...

Toxicant coated grain-based baits are widely used to control rodent pests throughout the world, but where alternative food sources are available, bait acceptance and efficacy are often less than optimal. In an attempt to develop baiting strategies to increase bait acceptance and efficacy of zinc phosphide-coated baits, a sugar-enhanced rolled oat b...

The mountain beaver is a semi-fossorial rodent of the Pacific Northwest and is among a variety of herbivores that retard plant growth and cause tree seedling deformities and mortality. Douglas-61 seedlings are planted in the Pacific Northwest from February through March, a period coinciding with mountain beaver parturition. Previous research sugges...

An analytical method employing hydrophobic interaction chromatography (HIC) combined with parallel detectors, provided a quantitative method for the determination of soluble carbohydrates in conifer needle tissues. Evaporative light‐scattering detection (ELSD), in parallel with atmospheric pressure chemical ionization mass spectrometry (APCI‐MS), y...

An analytical method is required for the analysis of monoterpenes in animal plasma to support a pharmacokinetic study. Monoterpenes common to sagebrush are extracted from sheep plasma by employing solid-phase extraction (SPE), followed by analysis of the extracts by gas chromatography with flame ionization detection. The analytes are quantitated ve...

Deer foraging on tree seedlings is recognized as the most widespread detriment to reforestation efforts. Non-lethal approaches to reduce deer damage to seedlings are highly desirable. Avoidance of natural secondary metabolites contained in conifers may provide feasible means to develop non-lethal measures. Other studies have demonstrated that sheep...

The widespread manufacture of plastics requires the similarly ubiquitous use of plasticizers. Plasticizers such as bis(2-ethylhexyl) adipate (DOA) and bis(2-ethylhexyl) phthalate (DOP) enhance polymer strength and flexibility and are found in polymeric products such as cosmetics, detergents, and building and storage products (1). However, these add...

20,25-Diazacholesterol is being evaluated as a contraceptive for the nonlethal control of avian and mammalian wildlife pests. The identification of an analyte in blood which was highly correlated with absorbed dose and efficacy is valuable for determining effective formulations and dosing variables. Such an analyte or biomarker is also valuable for...

1. Plant Metabolism 2. Phytochemical Variation in Plants 3. Plant Defense Theory 4. Mammalian Metabolism of Phytochemicals 5. Mammalian Herbivory Related Chapters

Since the early 1990's, an increase in damage associated with the activities of black (Coragyps atratus) and turkey (Cathartes aura) vultures has been observed. These activities include extensive damage to real and personal property. Vultures display an affinity for objects made of synthetic materials such as vinyl or plastic, and frequently damage...

We studied the persistence of capsicum oleoresin in a sandy loam soil. Five capsicum–soil concentrations (6.00%, 3.00%, 1.50%, 0.75%, and 0.00% wt : vol) were prepared in separate plastic boxes without lids; soils were elevated above wire-screen sub-floors to allow percolation and were exposed outdoors to ambient conditions. Plexiglass shields were...

Foraging on lettuce seeds and seedlings by horned larks (Eremophila alpestris) causes millions of dollars in losses to the California lettuce crop annually. Anthraquinone (AQ; 9,10-anthracenedione) has been shown to deter pest birds from consuming the seeds and seedlings of several plant species and was evaluated as a repellent to horned larks when...

We added a novel flavor, citric acid (CA), to a familiar test diet and conditioned an aversion in lambs to the flavored diet using lithium chloride. In 1 h feeding trials, we examined the roles of a novel flavor cue, choice and nutritional quality of alternatives on the persistence of an aversion. Availability of alternative foods increased the per...

The efficacy of three oral formulations (gelatin capsule, tablet, oil base) and five dosages (50, 100, 250, 500, 1000 microg) of cabergoline to disrupt reproduction in coyotes (Canis latrans) was evaluated. The type of formulation used had no effect on plasma progesterone and prolactin concentrations or on mean litter size. No adverse side effects...

An electrospray ionization tandem mass spectrometric method was developed for low-picogram detection of an ergot alkaloid, cabergoline, in coyote plasma extracts. Cabergoline is under investigation as an abortifacient in canid species. Central to the successful development of this method was the ability to introduce relatively large sample volumes...

Available evidence indicates that effective coyote attractants are blends of volatile substances. Typically, attractants are a combination of biological substances such as fermented glandular materials, urines, and rotted meats. Although effective, these attractants have several distinct disadvantages. Among these is the possibility that they are u...

The brown tree snake (Boiga irregularis) is an introduced pest in Guam, responsible for extensive agricultural damage, the extinction of several bird species, and severe and frequent electrical power outages. Rotenone, a naturally occurring pesticide, has been investigated as a possible chemical control agent. An analytical method was developed to...

In the traditional sense, food ingestion consists of prehending, masticating, swallowing, and digesting plant matter. It is also possible to ingest plants without eating them. Volatile compounds are inhaled directly into the lungs and transported from the lungs into the bloodstream. Volatiles in high concentrations could presumably produce toxicosi...

Silvicultural practices can in¯uence black bear (Ursus americanus) foraging preferences for Douglas-®r (Pseudotsuga menziesii) cambial-zone vascular tissues, but little is known about the role of genetics. To study the impact of genetic selection, vascular tissue samples were collected from Douglas-®r trees in six half-sib families from ®ve differe...

Deer Away Big Game Repellent(R) (BGR) effectively deters browsing deer, most likely by the release of sulfur odors and volatile fatty acids. Because many herbivores avoid these odors, a logical inference is that BGR may repel herbivores other than deer. To address this possibility, we tested whether BGR was repellent to eastern cottontail rabbits (...

The chemical constituents of coniferous vascular tissues play a role in bear forage selection. In particular, bear foraging preferences are related to the concentrations of simple sugars (nonstructural carbohydrates) and terpenes in the forage. Analyses of vascular tissue samples from trees collected in test plots indicated that both thinning and f...

The impact of live canopy pruning on the carbohydrate and terpene content of vascular tissue was investigated in the lower bole of Douglas-fir (Pseudotsuga menziesii). Cambial zone vascular tissue samples were collected from pruned and unpruned trees in the lower bole and within the live canopy. Current year's radial growth was estimated from the m...

The role of chemical constituents in the foraging behavior of black bears (Ursus americanas) was investigated using two field studies. Vascular tissue samples were collected from Douglas-fir (Pseudotsuga menziesii) trees recently foraged by black bears. Samples were extracted and analyzed by liquid and gas chromatography to determine carbohydrates...

Black bears forage on Douglas-fir vascular tissue in the spring, and this behavior can be severely detrimental to the health and economic value of a timber stand. Foraging is selective in that not all stands are damaged and, within a stand, one tree may be stripped while its neighbor is ignored or minimally sampled. A series of studies was conducte...

Coyotes were dosed with pentachlorobenzene (PeCB) and 1,2,4,5-tetrachlorobenzene (1,2,4,5-TeCB). Residues were determined in feces and adipose tissue at intervals up to six months post-dosing. PeCB was detectable in feces for six months post-dosing. 1,2,4,5-TeCB residues were of similar persistence and magnitude to those of PeCB. PeCB was metaboliz...