Brigitte Vannier

Brigitte Vannier
Université de Poitiers | UP · Pôle Biologie Santé

2rct.labo.univ-poitiers.fr

About

58
Publications
15,378
Reads
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1,449
Citations
Additional affiliations
September 2000 - present
September 1999 - present
Université de Poitiers
Position
  • Professor (Associate)
Description
  • PDE 6 Activity (1999-2006) Bioinformatics, R , Phytochemistry / Pharmacology/ anticancer activity
September 1999 - January 2015
Université de Poitiers
Position
  • Professor (Associate)
Education
September 1991 - October 1995
Université Paris-Sud 11
Field of study
  • Molecular Biology Endocrinology
September 1990 - September 1991
Université Paris-Sud 11
Field of study
  • Molecular Biology Endocrinology
September 1989 - September 1990
Sorbonne Université
Field of study

Publications

Publications (58)
Article
Full-text available
Antioxidant and anti-glycation properties of nine Moroccan plants of the Lamiaceae family used in traditional medicine, has been evaluated by after extraction by hot (HAE) or cold (CAE) maceration and by ethanol (EE). Anti-glycation activity was performed on BSA-Methylglyoxal system and was measured by fluorescence and native electrophoresis. The E...
Article
Conventional clustering algorithms optimize a single criterion, which may not conform to diverse needs of multidimensional data science. This paper proposes a new clustering algorithm that solves multiple clustering issues, called clustering by Marked Point Process (ClusterMPP). It is a new, efficient, scalable and unsupervised density-based cluster...
Article
Full-text available
Use of bioinformatics tools in life sciences have become necessary to analyze experimental data since omics have emerged in this field. The large amount of data is a challenge for the biological community as most biologists are not familiar to informatics and statistical interpretation and concepts. An interdisciplinary collaboration is welcome to...
Article
Full-text available
The EGF-family of tyrosine-kinase receptors activates cytoplasmic pathways involved in cell proliferation, migration and differentiation in response to specific extracellular ligands. Beside these canonical pathways, the nuclear localization of the ErbB receptors in primary tumours and cancer cell lines led to investigate their role as transcriptio...
Article
Full-text available
The present work describes the isolation and anticancer activity of Tapinanthus sp. which is a hemi parasitic plant harvested on Combretum glutinosum, the host plant. Phytochemical study afforded a new flavonoid glycoside, tapinantoside (1) isolated for the first time from natural source, alongside six known compounds (2-7). Structure of compounds...
Article
Full-text available
Background: Cancer incidence has been growing in an alarming rate worldwide and new therapeutics are needed, particularly for intractable and chemoresistant cases. We evaluated the cytotoxic effects of Combretum fragrans F. Hoffm (Combretaceae) on glioblastoma (U87MG and C6) and prostate (PC-3) cancer cell lines. Methods: The cytotoxic effect of...
Article
Full-text available
A new phenyl alkyl ester and a new combretin triterpene derivative from Combretum fragrans F. Hoffm (Combretaceae) and antiproliferative activity Abstract: This work concerns the isolation and structure elucidation of compounds obtained from the extract of leaves and stem bark of Combretum fragrans F. Hoffm. Both extracts and some isolated compound...
Article
Clustering via marked point processes and influence space, Is-ClusterMPP, is a new unsupervised clustering algorithm through adaptive MCMC sampling of a marked point processes of interacting balls. The designed Gibbs energy cost function makes use of k-influence space information. It detects clusters of different shapes, sizes and unbalanced local...
Chapter
Microarray technologies become a powerful technique for simultaneously monitoring expression patterns of thousands of genes under different conditions. However, it is important to identify gene groups that manifest similar expression profiles and are activated by similar conditions. ClusterMPP: Clustering by Marked Point Process is a new microarray...
Article
Full-text available
CD36 is a multifunctional membrane-type receptor glycoprotein that reacts with oxidized low-density lipoprotein and long-chain fatty acid (LCFA). However, much remains to be understood about the molecular mechanism of the cardio-myopathy observed in CD36-KO mice. In this study, we identify different genes pathways involved in response to CD36 cardi...
Article
Full-text available
CD36 is a multifunctional membrane-type receptor glycoprotein that reacts with oxidized low-density lipoprotein and long-chain fatty acid (LCFA). However, much remains to be understood about the molecular mechanism of the cardio-myopathy observed in CD36-KO mice. In this study, we identify different genes pathways involved in response to CD36 cardi...
Data
Transcriptional effects of the nuclear ErbB380kDa isoform at the protein level. PC3 cells were transiently transfected with si control, siErbB3A or siErbB3B 48h before cytosolic (C) and nuclear (N) extraction. Western blotting was performed using antibodies to PPIG (sc-100699, SCBT), GATA2 (sc-9008, SCBT), RUNX2 (sc-10758, SCBT), CCND1 (#2922, CST)...
Article
Cytotoxic effects of an aqueous extract of a plant used in traditional Congolese medicine Klainedoxa gabonensis Pierre ex Engl. (KG) were studied on the aggressive U87 glioblastoma cell line. An aqueous extract (E2) was prepared from barks of KG and tested at different concentrations using an MTT assay (IC50 of 65μg/ml). The E2 KG extract exerts a...
Chapter
This chapter briefly discusses how to extract signal from microarray data. It then considers the problem of differentially expressed (DE) genes detection in a replicated experiment. The main goal of both intraplatform and interplatform microarray technologies can be achieved in detecting DE genes when the microarray experiment is performed appropri...
Article
Full-text available
Introduction: We have carried out our study on the chemical composition; anti-glycation andradical scavenging activities of Origanum compactum, Rosmarinus officinalis and Pelargonium asperum essential oils (EO) that were harvested in the northeast of Morocco. These plants have been traditionally used in medicine as a chief ingredient of many polyhe...
Article
Full-text available
Microarray experiments enable the simultaneous measure of expression levels of large amount of genes and have many applications. A widespread one is finding set of genes that are differentially expressed. Significance Analysis of Microarrays (SAM) helps to produce those sets using multiple testing techniques. There is unfortunately not yet a public...
Article
Full-text available
Abstract: In response to the rapid development of DNA Microarray Technologies, many differentially expressed genes selection algorithms have been developed, and different comparison studies of these algorithms have been done. However, it is not clear how these methods compare with each other, especially when we used different developments tools. He...
Article
Full-text available
In response to the rapid development of DNA Microarray Technologies, many differentially expressed genes selection algorithms have been developed, and different comparison studies of these algorithms have been done. However, it is not clear how these methods compare with each other, especially when we used different developments tools. Here, we c...
Article
Full-text available
We have previously shown that CD36 is a membrane protein that facilitates long chain fatty acid (FA) transport by muscle tissues. We also documented the significant impact of muscle CD36 expression on heart function, skeletal muscle insulin sensitivity as well as on overall metabolism. To identify a comprehensive set of genes that are differentiall...
Article
Full-text available
Saturated Fatty Acids (SFA) have been reported to alter organelle integrity and function in many cell types, including muscle and pancreatic β-cells, adipocytes, hepatocytes and cardiomyocytes. SFA accumulation results in increased amounts of ceramides/sphingolipids and saturated Phospholipids (PL). In the present study, using a yeast-based model t...
Conference Paper
Full-text available
La plateforme de programmation Matlab propose des outils de lecture, de traitement et d’analyse des biopuces. La recherche de gènes différentiellement exprimés est une des méthodes que propose cette plateforme, mais jusqu'à ce jour, elle n’a pas fourni un moyen d’appliquer l’algorithme SAM (Significance Analysis of MicroArray) . Cependant le SAM re...
Article
Full-text available
We have previously shown that CD36 is a membrane protein that facilitates long chain fatty acid (FA) transport by muscle tissues. We also documented the significant impact of muscle CD36 expression on heart function, skeletal muscle insulin sensitivity as well as on overall metabolism. To identify a comprehensive set of genes that are differentiall...
Article
Full-text available
This paper proposes our algorithm for gene selection in microarray data analysis comparing conditions with replicates. Based on background noise computation in replicate array, this algorithm uses the global False Discovery Rate based on 'Between' group and 'Within' group comparisons of replicates to select the set of differential expressed genes....
Conference Paper
Motivation: In the analysis of experiments that involves the high density of oligonucleotide chips, it is important to generate list of genes or 'targets' from the genome wide data set that contains a lot of information. Gene selection is a process that seeks to identify the most significant genes which reveal large expressions changes between the...
Article
Full-text available
This paper proposes the authors’ algorithm for gene selec tion in microarray data analys is comparing conditions with replicates. Based on background noise computa tion in replicated arrays, this algorithm uses the global False Discovery Rate bas ed on ‘Inter’ and ‘Intra’ group comparisons of re plicates to select different ial expressed gene sets....
Conference Paper
Full-text available
This paper proposes our algorithm for gene selection in microarray data analysis comparing conditions with replicates. Based on background noise computation in replicate array, this algorithm uses the global False Discovery Rate based on ‘Between ’ group and ‘Within ’ group comparisons of replicates to select the set of differential expressed genes...
Article
Full-text available
In photoreceptors, phosphodiesterase 6 (PDE6) is regulated in response to light, due to the shuttling of a regulatory subunit (PDE6gamma) between the catalytic subunits of PDE6 and the activated form of transducin. We showed previously that PDE6gamma is able to interact with the Src-homology type 3 (SH3) domain of formin-binding protein 17 (FBP17),...
Article
Full-text available
Phosphodiesterase 6 (PDE6) is the primary effector of phototransduction in vertebrate photoreceptors. Previous studies described the expression of the regulatory subunit of rod PDE6 (Pgamma-rod) in non-photosensitive tissues of the adult rat and the effects of this protein on MAP kinase pathways. Upon examination of the Pgamma-rod sequence, we dete...
Article
Full-text available
The regulatory mechanisms of oocyte maturation remain poorly understood. Although gonadotropins play a major role in these processes, they have generally been considered to act on somatic supportive cells, but not directly on germ cells. We have raised high affinity monoclonal antibodies against LH and FSH receptors. When using the latter to study...
Article
Full-text available
Gonadotropin receptors belong to a subgroup of G-protein coupled receptors characterized by a large extracellular domain responsible for the binding of the hormone. Soluble, hormone-binding, alternative splicing variants of the LH receptor, are present in high concentration. A mannose rich precursor form of LH and FSH receptor is accumulated inside...
Article
Full-text available
Capacitative Ca2+ entry (CCE) is Ca2+ entering after stimulation of inositol 1,4,5-trisphosphate (IP3) formation and initiation of Ca2+ store depletion. One hallmark of CCE is that it can also be triggered merely by store depletion, as occurs after inhibition of internal Ca2+ pumps with thapsigargin. Evidence has accumulated in support of a role of...
Article
Full-text available
The follicle-stimulating hormone receptor (FSHR) is physiologically localized in the basolateral compartment of the membrane of Sertoli cells. This localization is also observed when the receptor is experimentally expressed in Madin-Darby canine kidney cells. We thus used in vitro mutagenesis and transfection into these polarized cells to delineate...
Article
Full-text available
Transient receptor potential (Trp) proteins form ion channels implicated in the calcium entry observed after stimulation of the phospholipase C pathway. Kyte-Doolittle analysis of the amino acid sequence of Trp proteins identifies seven hydrophobic regions (H1-H7) with potential of forming transmembrane segments. A limited sequence similarity to vo...
Article
Full-text available
The thyrotropin (TSH) and follicle-stimulating hormone (FSH) receptors are present mainly on the basolateral cell surface in the thyroid gland and in Sertoli cells, whereas in ovarian and in testicular cells, the luteinizing hormone (LH) receptors are distributed throughout the cell surface. When expressed in Madin-Darby canine kidney (MDCK) cells,...
Article
Gonadotrophin and thyrotrophin receptors belong to a subgroup of G-protein-coupled receptors. These receptors are characterized by a large extracellular domain that is responsible for the binding of the hormone. Soluble receptors, such as some luteinizing hormone receptors, arise from premessenger RNA alternative splicing, or, in the case of thyroi...
Article
Monoclonal antibodies have been raised against the LH/CG receptor [1] and have allowed to perform immunochemical studies of the receptor in target cells. Three different forms of the LH/CG receptor are physiologically expressed: a mature approximately 85 kDa transmembrane species corresponding to the full length receptor, a approximately 68 kDa hig...
Article
Full-text available
Gonadotrophin and thyrotrophin receptors belong to a subgroup of G-protein-coupled receptors. These receptors are characterized by a large extracellular domain that is responsible for the binding of the hormone. Soluble receptors, such as some luteinizing hormone receptors, arise from premessenger RNA alternative splicing, or, in the case of thyroi...
Article
Gonadotropin and thyrostimulin receptors belong to a subgroup of G- protein coupled receptors characterized by a large extracellular domain responsible for the binding of the hormone. Soluble receptors arise from pre- messenger RIVA alternative splicing (LH receptor) or cleavage and shedding of the ectodomain (TSH receptor). FSH and TSH receptors a...
Article
Full-text available
During the last 2 years, our laboratory has worked on the elucidation of the molecular basis of capacitative calcium entry (CCE) into cells. Specifically, we tested the hypothesis that CCE channels are formed of subunits encoded in genes related to the Drosophila trp gene. The first step in this pursuit was to search for mammalian trp genes. We fou...
Article
Full-text available
The extracellular domain of the human FSH receptor was expressed in Escherichia coli as a fusion protein with ubiquitin. It was tagged with a poly-His tract which was used for its purification. Immunization of mice allowed the preparation of high affinity antireceptor monoclonal antibodies. The latter fell into two categories: some of them were inh...
Article
Full-text available
The extracellular and intracellular domains of the human thyrotropin receptor were expressed in Escherichia coli and the proteins were used to produce monoclonal anti-receptor antibodies. Immunoblot studies and immunoaffinity purification showed that the receptor is composed of two subunits linked by disulfide bridges and probably derived by proteo...

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