Brian R Murphy

Brian R Murphy
Trinity College Dublin | TCD · Department of Botany

RSci, MRSB, M.I.Biol.I., B.A. Hons., PhD, Botany

About

65
Publications
17,463
Reads
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530
Citations
Citations since 2016
38 Research Items
481 Citations
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2016201720182019202020212022020406080100
2016201720182019202020212022020406080100
2016201720182019202020212022020406080100
Additional affiliations
August 2021 - present
CropBiome Ltd
Position
  • Consultant
Description
  • Brian Murphy is a Founder, Director and Senior Scientific Adviser with the newly launched biotech company CropBiome Ltd. (www.cropbiome.com). CropBiome is currently engaged in a series of projects examining the commercial potential of naturally occurring microorganisms to improve the growth and yield of field grown cereal crops. We are also extending the scope of our research to other important crop species.
September 2015 - November 2016
Trinity College Dublin
Position
  • Research Associate

Publications

Publications (65)
Article
Full-text available
Fraxinus excelsior populations are in decline due to the ash dieback disease Hymenoscyphus fraxineus. It is important to understand genotypic and environmental effects on its fungal microbiome to develop disease management strategies. To do this, we used culture dependent and culture independent approaches to characterize endophyte material from co...
Article
Full-text available
Bioremediation is an ecologically-friendly approach for the restoration of heavy metal-contaminated sites and can exploit environmental microorganisms such as bacteria and fungi. These microorganisms are capable of removing and/or deactivating pollutants from contaminated substrates through biological and chemical reactions. Moreover, they interact...
Article
Full-text available
Strawberries are important global soft-fruit crop, but optimal production is constrained by the diseases and chemical treatments. Grey mould disease, caused by the pathogen Botrytis cinerea, is a devastating pathogen of strawberry crops worldwide. In this study,we recovered fungal leaf endophytes from a wild relative of strawberry, Fragaria vesca,...
Chapter
Endophytes for a Growing World - edited by Trevor R. Hodkinson March 2019
Book
The book brings together papers covering the most recent scientific research from the top endophyte researchers in the world. It presents the state of the art in our knowledge and technical capacity and explores future directions of this work. It is highly relevant and timely because of the need to improve global food security and its sustainabilit...
Chapter
Endophytes are any microbes that can live within plants. We divide them into three major groups: endosyms (endosymbionts ), endopaths (pathogens) and endosympaths (those that exist in both forms along a mutualism – parasitism continuum). Within these groups, endophytologists recognise harmful pathogenic microbes and a diverse range of beneficial/ c...
Chapter
In vitro tissue culture systems are required for plant– microbe interaction studies on European ash, Fraxinus excelsior . Methods are needed for plant micropropagation and for physiological experimentation including pathogen / resistance testing and biocontrol studies. For example, systems are required for experiments on ash dieback disease, caused...
Chapter
The wild relatives of agricultural crops represent a largely untapped source of beneficial microbial endophytes that have potential for agricultural applications. Much of the research into the effects of endophytes on crop species has focused on a relatively small selection of well- characterised bacterial or fungal strains. However, many of these...
Chapter
Barley is an important crop worldwide with production largely used for animal feed and alcoholic beverages. Diseases are a major limiting factor to its production. These have, up until recently, been controlled by agrochemicals. However, legislation on the use of agrochemicals, especially within the European Union, is being tightened and there is g...
Chapter
Endophytes have the potential to contribute to the sustainable production of bioenergy crops such as the perennial rhizomatous grass Miscanthus . They can improve plant growth on marginal land that is otherwise unsuitable for conventional agriculture and can also reduce the need for environmentally damaging chemical inputs including fertilisers and...
Article
Full-text available
Endophytes are microorganisms that spend all or part of their lives inside plant tissue without causing disease symptoms. Strains of fungal endophytes have been shown to have great potential as beneficial crop inoculants for biocontrol, biofertilisation and biostimulation. In this study, we carried out rep-licated glasshouse trials to examine the e...
Chapter
Full-text available
Throughout their existence on Earth, fungi and plants have been intricately linked, and their interactions span a vast continuum from detrimental to beneficial. Although negative interactions have received more attention, due in part to their impacts on crops, forests and horticulturally important plants, positive plant–fungal interactions are not...
Article
Full-text available
Agricultural crops growing in saline soils produce sub-optimal yields due to the negative effects of excess salts on plant growth and development. Salinity stress for crops is likely to increase as a result of climate change and the consequent salinisation of soils, particularly in coastal areas. In this study, we recovered fungal endophytes from t...
Chapter
Full-text available
Although negative interactions have received more attention, due in part to their impacts on crops, forests and horticulturally important plants, positive plant–fungal interactions are not only ubiquitous but also vitally important to sustaining life on this planet. In this chapter, we highlight what is currently known about the beneficial interact...
Article
Full-text available
The rapid development of high-throughput techniques and expansion of bacterial databases have accelerated efforts to bring plant microbiomes into cultivation. We introduced plant-only-based culture media as a successful candidate to mimic the nutritional matrices of plant roots. We herein employed a G3 PhyloChip microarray to meticulously character...
Conference Paper
Full-text available
The use of chemicals to fertilize crops incurs economic and environmental costs and it is widely recognized that the current level of chemical fertilizer use is unsustainable in many intensive farming systems. Any methods that can reduce fertilizer input and still maintain acceptable yields would be of great benefit to both the farmer and the envir...
Article
Full-text available
The use of chemicals to fertilize crops incurs economic and environmental costs and it is widely recognized that the current level of chemical fertilizer use is unsustainable in many intensive farming systems. Any methods that can reduce fertilizer input and still maintain acceptable yields would be of great benefit to both the farmer and the envir...
Article
Full-text available
Environmental stresses are limiting factors in optimal agricultural crop yield, and these stresses, especially drought, are likely to become more acute due to future climate change. Crop wild relatives contain environmentally selected endophytes that can help to increase stress resistance. Our previous work with barley cultivars has shown a positiv...
Article
Full-text available
The development of endophyte inoculants for agricultural crops has been bedevilled by the twin problems of a lack of reliability and consistency, with a consequent lack of belief among end users in the efficacy of such treatments. We have developed a successful research pipeline for the production of a reliable, consistent and environmentally targe...
Conference Paper
The use of beneficial fungi as crop inoculants is constrained by the need for application of fungicides to most field-grown crops. The fungicides often suppress the ‘good’ as well as the ‘bad’ fungi, reducing the ability of the beneficial fungi to benefit the crop. Root endophytic fungi have been shown to improve agronomic traits in spring barley a...
Conference Paper
The wild relatives of agricultural crops represent a tremendous and largely untapped source of beneficial endophytes that have potential for agricultural applications. Much of the research into the effects of endophytes on crop species have focused on a relatively small selection of well-characterised strains. However, many of these strains can hav...
Article
The use of chemicals to fertilize crops incurs economic and environmental costs and it is widely recognized that the current level of chemical fertilizer use is unsustainable in many intensive farming systems. Any methods that can reduce fertilizer input and still maintain acceptable yields would be of great benefit to both the farmer and the envir...
Article
Full-text available
Plant-fed versus chemicals-fed rhizobacteria of Lucerne: Plant-only teabags culture media not only increase culturability of rhizobacteria but also recover a previously uncultured Lysobacter sp., Novosphingobium sp. and Pedobacter sp. Abstract In an effort to axenically culture the previously uncultivable populations of the rhizobacteria of Lucerne...
Conference Paper
Endophytes associated with crops have potential as beneficial inoculants in agriculture, but little is known of their genetic diversity and phylogenetic relationships. We carried out the first ever ecological and phylogenetic survey of the culturable fungal root endophytes of a wild barley species. Fungal root endophytes were isolated from ten popu...
Article
Full-text available
The potential of fungal root endophytes to suppress vertically transmitted infections in barley cultivars has only been studied in relatively few symbiotic associations, and is virtually unknown for endophytes derived from wild barley species. Here, we show that fungal root endophytes derived from wild populations of Hordeum murinum ssp. murinum L....
Article
Full-text available
The use of beneficial fungi as crop inoculants is constrained by the need for application of fungicides because the pathogen controlling fungicides often concurrently suppress the efficacy of the beneficial mycobiont. Root endophytic fungi have been shown to improve agronomic traits in spring barley and may be less sensitive to the regularly applie...
Conference Paper
Fungal and bacterial endophytes show great promise as biocontrol and biofertilisation organisms in agriculture. However, most of the research which has demonstrated endophyte benefits for crops has been conducted in controlled environments, and the few field experiments that have been carried out have produced inconsistent results. While we underst...
Article
The use of bryophyte and lichen cover as a proxy for air relative humidity (RH) and temperature in tropical forests has been widely proposed. Many studies that have assessed the usefulness of such indicators have mostly focused on estimates from ground observations. Here we identify the usefulness of bryophyte and lichen cover to estimate RH and te...
Article
Full-text available
Endophytes associated with crops have potential as beneficial inoculants in agriculture, but little is known about their genetic diversity and phylogenetic relationships. We carried out the first ever ecological and phylogenetic survey of the culturable fungal root endophytes of a wild barley species. Fungal root endophytes were isolated from 10 po...
Conference Paper
Barley is subject to many pathogenic infections which cause significant economic losses. Growers have used an ever-changing arsenal of chemicals in an effort to control these pathogens. As well as being economically costly, chemical crop treatments for pathogens can have severe and long-lasting negative effects on the environment and reduce biodive...
Article
Crops often face severe and damaging local drought events and in some regions these episodes are predicted to become more frequent due to climate change. Some micro-organisms have been shown to improve drought tolerance and improve yield in crop plants. Here, we show that fungal root endophytes isolated from a wild barley species (Hordeum murinum s...
Article
Successfully addressing the challenge of providing future food security will require both improvements in crop yield as well as the cultivation of additional farmland. This may result in the steady increase of farming on marginal, arid, and semi-arid lands, especially in the developing world, leading in turn to greater biotic and abiotic stresses o...
Article
Full-text available
Fungal endophytes have great potential as biocontrol and biofertilisation agents for food crops, and it is important to understand and optimise the cultural conditions for endophyte emergence and growth. A range of commercial culture media were tested for their effect on endophyte recovery from the roots of their host (Hordeum murinum), and for the...
Conference Paper
Successfully addressing the challenge of providing future food security will require both improvements in crop yield as well as the cultivation of additional farmland. This may result in the steady increase of farming on marginal, arid, and semi-arid lands, especially in the developing world, leading in turn to greater biotic and abiotic stresses o...
Article
Overuse of chemical fertilisers in barley crops carries large economic and environmental costs and can lead to ecosystem degradation and loss of biodiversity. Methods of reducing chemical crop inputs using endophyte treatments have been demonstrated elsewhere. Here, we show that inoculation with six different fungal root endophytes isolated from wi...
Article
Full-text available
Fungal endophytes have great potential as biocontrol and biofertilisation agents for food crops, and it is important to understand and optimise the cultural conditions for endophyte emergence and growth. A range of commercial culture media were tested for their effect on endophyte recovery from the roots of their host (Hordeum murinum), and for...
Article
Full-text available
Abstract- Sustainable farming systems are required to allow crops to better cope with the simultaneous multiple stresses that they grow under or are likely to be exposed to under future climate change. Fungal endophytes could form part of the solution. They have been shown to improve important agronomic traits under a single stress, but few st...
Article
Ten fungal root endophytes were isolated from wild populations of Hordeum murinum ssp. murinum L. and inoculated onto untreated seeds of a barley cultivar using five artificial and one soil-based growth media. A co-inoculant of all ten isolates as well as two individual isolates successfully suppressed the development of seed-borne fungal infection...
Conference Paper
Plants respond to actual and potential colonisation by root associated fungal endophytes using both internal and external signals. Here, we show that exogenous environmental signals associated with stress resulted in increased fungal root endophyte recruitment, and are thus the first link with endogenous downstream signalling in the plant resulting...
Article
Full-text available
The effective management and maintenance of a dry lowland heath ecosystem requires detailed knowledge of thcontributing environmental factors. In order to determine the major controls in such an ecosystem, a series of floristic relevés on thheathland and secondary woodland vegetation of the north-west side of the Ben of Howth, Howth, County Dublin,...
Article
Full-text available
Sebacinales are a large, potentially useful and ecologically diverse order of fungi that requires in-depth DNA sequencing for barcoding and phylogenetics, along with accurate morphological data. For identification using sequences it is essential to have data from well classified and studied reference collections so that genotype and phenotype can b...
Conference Paper
Fungal infections of crops are often devastating and costly. However, not all fungal infections are detrimental, and some are beneficial. Beneficial root infections often involve symbiotic root endophytes. Benefits to plants infected with endophytic root fungi include an increase in seed yield, enhanced resistance to pathogens and improved stress t...
Article
Full-text available
The effective management and maintenance of a dry lowland heath ecosystem requires detailed knowledge of the contributing environmental factors. In order to determine the major controls in such an ecosystem, a series of floristic relevés on the heathland and secondary woodland vegetation of the north-west side of the Ben of Howth, Howth, County Dub...
Article
Full-text available
Sebacinales are a large, potentially useful and ecologically diverse order of fungi that requires in-depth DNA sequencing for barcoding and phylogenetics, along with accurate morphological data. For identification using sequences it is essential to have data from well classified and studied reference collections so that genotype and phenotype can b...
Article
Full-text available
Purpose: The fungus Piriformospora indica was first isolated from plants growing in arid, hot desert conditions and has been shown to have significant potential as a biocontrol and biofertilising organism in barley under optimal growth conditions. However, it was not thought to be effective in plants grown in low temperatures and has consequently n...
Article
The fungal root endophytes Chaetomium globosum, Epicoccum nigrumand Piriformospora indicahave value as biocontrol and biofertilising organisms in barley, but have not been well tested at low temperatures. This study assessed the efficacy of the endophytes on barley varieties grown under low temperature stress with variable nutrient input. Seed from...
Article
Pathogenic fungal infections of barley can lead to costly crop losses. However, not all fungal infections are detrimental, and some are even beneficial. Beneficial root infections often involve symbiotic endophytic fungi. Benefits to barley and other plants infected with endophytic root fungi include an increase in seed yield, enhanced resistance t...
Article
This review presents the evidence for regarding fungal root infections in barley as friends as well as foes. Despite a widespread perception that fungal root endophytes will always confer benefits on their hosts, this review and other meta-analyses reveal among-study contradictions, probably due to experimental variability. Endophyte infection can...
Article
Fungal infections of barley have tremendous agricultural significance, and can be detrimental or beneficial. Beneficial root infections often involve endophytic fungi, but endophytic associations do not always confer benefits on their hosts. Endophyte infection can be negative, positive or neutral for the host. Benefits to barley and other plants i...

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Projects

Projects (3)
Project
The project is focused on the isolation and identification of beneficial endophytes to introduce in agronomic crops to stimulate their growth in stress condition, biotic and abiotic, with the prospective to reduce the amount of chemical input released into the soil.
Project
Trinity College Dublin are hosting ‘Sustain: Endophytes for a Growing World’, an international conference taking place from 28th to 29th August 2017 (Website: https://www.tcd.ie/Botany/sustain2017/sustain2017.php). The two-day conference will be centred on the core themes of manipulating the plant microbiome for sustainable plant production in a changing world. Climate change will present formidable problems for many areas of plant production, from agriculture to forestry and horticulture. Understanding the potential beneficial role of endophytes in helping plants to cope with the associated stresses will be a crucial factor in future sustainable plant production practices. The conference will highlight cutting-edge research using endophytes and will synthesise the most promising approaches to endophyte supported plant production.
Project
Our goal is to recover, test and commercialise novel endophyte strains for agricultural crops