Bradley LuskAmerican Association for the Advancement of Science | AAAS · USAID
Bradley Lusk
PhD, Biological Design
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24
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Introduction
Additional affiliations
August 2009 - December 2015
Publications
Publications (24)
Platinum group metal (PGM) miners and recycling facilities are losing ~$2 billion (~10% of the total market value) of PGM annually in wastewater and tailing. The membrane biofilm reactor (MBfR) is a modular biotechnology which contains a microbial community that can reduce and recover PGM at mining, refining, or manufacturing sites at concentration...
Here we present a new text analysis tool that consists of a text analysis service and an author search service. These services were created by using or extending many existing Free and Open Source tools, including streamlit, requests, WordCloud, TextStat, and The Natural Language Tool Kit. The tool has the capability to retrieve journal hosting lin...
We analyzed the microbial community of multicolored speleosol deposits found in Grand Canyon Caverns, a dry sulfuric karst cave in northwest Arizona, USA. Underground cave and karst systems harbor a great range of microbial diversity; however, the inhabitants of dry sulfuric karst caves, including extremophiles, remain poorly understood. Understand...
Approximately four billion years ago, the first microorganisms to thrive on earth were anaerobic chemoautotrophic thermophiles, a specific group of extremophiles that survive and operate at temperatures ∼50 – 125°C and do not use molecular oxygen (O2) for respiration. Instead, these microorganisms performed respiration via dissimilatory metal reduc...
In the 21st century, conversations regarding responsible research and innovation (RRI) place a strong emphasis on the need for inclusion of historically underrepresented communities in the science, technology, engineering, and math (STEM) disciplines. However, many of the current methodologies for integration with STEM require members of underrepre...
Platinum group metal (PGM) miners and recycling facilities are losing ~$2 billion (~10% of the total market value) of PGM annually in wastewater and tailing. The membrane biofilm reactor (MBfR) is a modular biotechnology which contains a microbial community that can reduce and recover PGMs and potentially precious metals inclduing gold (Au) at mini...
In this study, anode biofilms of the thermophilic (60 °C) Gram-positive anode-respiring bacterium (ARB) Thermincola ferriacetica were studied to determine the presence of a proton-dependent electron transfer response. The effects of pH, the presence of an electron donor (acetate), and biofilm growth were varied to determine their influence on the e...
An enriched mixed culture of thermophilic (60°C) bacteria was assembled for the purpose of using cellulose to produce current in thermophilic microbial electrolysis cells (MECs). Cellulose was fermented into sugars and acids before being consumed by anode-respiring bacteria (ARB) for current production. Current densities (j) were sustained at 6.5 ±...
Fig. S1. (a–f) Representative fermentation profiles tracked over 11 days from six serum bottles.
Fig. S2. Picture of a thermophilic H‐type microbial electrochemical cell in an incubator at 60 °C.
Fig. S3. CLSM LIVE/DEAD analysis revealed a live biofilm layer (Lf shown in yellow) approximately 40‐60 μm thick.
We assessed the effects of pH and buffer concentration on current production and growth of biofilms of Thermincola ferriacetica – a thermophilic, Gram-positive, anode-respiring bacterium (ARB) – grown on anodes poised at a potential of −0.06 V vs. SHE in microbial electrolysis cells (MECs) at 60 °C. T. ferriacetica generated current in the pH range...
Anode-respiring bacteria (ARB) catalyze the complete oxidation of organic compounds (e.g. acetate, glucose) into electrical current and carbon dioxide. ARB naturally produce a biofilm at the electrode surface of up to 100 micrometers, where even cells on the outer part of the biofilm are participating in current production. The specific pathway by...
Thermoanaerobacter pseudethanolicus 39E (ATCC 33223), a thermophilic, Fe(III)-reducing, and fermentative bacterium, was evaluated for its ability to produce current from four electron donors - xylose, glucose, cellobiose, and acetate -- with a fixed anode potential (+ 0.042 V vs. SHE) in a microbial electrochemical cell (MXC). Under thermophilic co...
A 3.19-Mbp draft genome of the Gram-positive thermophilic iron-reducing
Firmicutes
isolate from the
Peptococcaceae
family,
Thermincola ferriacetica
Z-0001, was assembled at ~100× coverage from 100-bp paired-end Illumina reads. The draft genome contains 3,274 predicted genes (3,187 protein coding genes) and putative multiheme
c
-type cytochromes.
Thermoanaerobacter pseudethanolicus (ATCC 33223) was studied for its electrochemical properties using an H-type Microbial Electrolysis Cell (MEC) reactor. Four electron donors were evaluated in this study e.g. cellobiose, glucose, xylose and acetate. Results showed that this microbe simultaneously ferments a wide range of hexose and pentose sugars...
Thermincola ferriacetica is a recently isolated thermophilic, dissimilatory Fe(III)-reducing, Gram-positive bacterium with capability to generate electrical current via anode respiration. Our goals were to determine the maximum rates of anode respiration by T. ferriacetica, and to perform a detailed microscopic and electrochemical characterization...