Botond Siklodi

CEVA · Analytical Method Transfer Platform

During the production of clostridial vaccines large numbers of mice are used for various in-process control tests. Replacement in vitro assays had been developed for the testing of the toxins and toxoids of several clostridial species, but none of these assays had been assessed in an international collaborative study. Under the common aegis of the...

Inter-laboratory study validation of in vitro toxicity and antigenicity assays for Clostridium septicum vaccine antigens Botond Siklodi1, Keith Redhead2, Arnold Daas3, David Le Tallec3, Lukas Bruckner4 and Marie-Emmanuelle Behr-Gross3 1Ceva-Phylaxia Veterinary Biologicals Co. Ltd., Hungary; 2Vaccine & Assay Consultancy Ltd., United Kingdom; 3Counc...

A new member of the chicken TNF superfamily has recently been identified, namely receptor activator of NF-κB ligand (RANKL), as have its signalling receptor, RANK, and its decoy receptor, osteoprotegerin (OPG). In mammals, RANKL and RANK are transmembrane proteins expressed on the surface of Th1 cells and dendritic cells (DC) respectively, whereas...

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We observed a remarkable synergism of adenoviruses and LPS in triggering the production of TNF in intact animals. We found that in mice pre-exposed to adenoviruses, LPS injections generated extremely high levels of TNF with altered kinetics. The elevated TNF synthesis stemmed mostly from posttranscriptional up-regulation of TNF production, although...

The effect of peptide length on the stability of peptide-HLR-DR1 (DR1) complexes was analyzed using two peptide series of increasing length, each containing a 7mer core with five DR1-binding anchors, extended stepwise with A1a residues at the N- and C-terminus, respectively. The A1a extensions, although did not affect binding affinity, significantl...

Transgenic (Tg) mouse models are unique tools for investigating regulatory mechanisms of the immune system. Mice bearing a T cell receptor (TCR) BV8S2 transgene derived from an encephalitogenic T cell clone are highly susceptible to experimental autoimmune encephalomyelitis (EAE), a T cell-mediated neurological disorder. Although the pathogenesis o...

Novel single chain MHC class II constructs consisting of only the β1 and α1 domains were produced in E. coli covalently linked to an encephalitogenic peptide known to induce T cell mediated experimental autoimmune encephalomyelitis (EAE) in Lewis rats, or complexed with these peptides after purification. The β1α1/MBP-72-89 complex bound specificall...

A panel of 26 monoclonal antibodies (mAbs) against glycoprotein B (gB) of Aujeszky's disease (pseudorabies) virus (ADV), a glycoprotein complex consisting of three glycoproteins, gBa, gBb, and gBc, was produced by two research groups and was used for the topographical epitope mapping of gB. An epitope map was constructed in which the identified epi...

The effect of peptide length on the lifespan of peptide-MHC class II complexes on live antigen presenting cells (APC) has been investigated using a designer peptide series of 8-12 amino acid residues in length. The affinity of these peptides for HLA-DRB1*0101 and HLA-DRB1*0401 in solution was independent of their length. However, the stability of t...

Certain HLA class II-specific monoclonal antibodies (mAb) cause up to 90% decrease in the cell surface expression of class II molecules. This down-regulation is isotype-specific, i.e. DR-specific mAb do not affect the expression of DP and DQ molecules. However, antibodies binding to one DR allotype down-regulate both allotypes in heterozygous antig...

A simple immunization method to obtain high-affinity monoclonal antibodies to progesterone is described in this article. The method is based on the theory of affinity maturation. A long interval between antigen priming and booster ("latent immunization") permits an undisturbed completion of affinity maturation, resulting in the accumulation of memo...