Boris Rubinsky

Boris Rubinsky
University of California, Berkeley | UCB · Department of Mechanical Engineering

PhD, MIT, 1981

About

469
Publications
87,687
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16,455
Citations
Additional affiliations
June 1986 - June 1987
University of Cambridge
Position
  • Visiting Fellow
June 1980 - present
University of California, Berkeley
Position
  • Professor (Full)

Publications

Publications (469)
Article
A 3D printing technology that facilitates continuous printing along a combination of cartesian and curvilinear coordinates, designed for in vivo and in situ bioprinting is introduced. The combined cartesian/curvilinear printing head motion is accomplished by attaching a biomimetic, flexible, "tendon cable" soft robot arm to a conventional cartesian...
Article
Full-text available
The eutectic is fundamental to multicomponent systems, defining the lowest temperature at which a given liquid phase is stable. Although binary aqueous eutectics have been broadly characterized at atmospheric pressure, eutectic data at high pressure are scarce, leaving critical gaps both in the data available for modeling and in our understanding o...
Article
Full-text available
Aqueous supercooling provides a method by which to preserve biological matter at subfreezing temperatures without the deleterious effects of ice formation. The extended longevity of the preserved biologic is a direct result of a reduction in the rate of metabolism with decreasing temperature. However, because the nucleation of ice from a supercoole...
Article
Stable aqueous supercooling has shown significant potential as a technique for human tissue preservation, food cold storage, conservation biology, and beyond, but its stochastic nature has made its translation outside the laboratory difficult. In this work, we present an isochoric nucleation detection (INDe) platform for automated, high-throughput...
Article
Full-text available
Background Tumor Treating Fields (TTFields), are a novel, non-invasive tissue ablation technology for treatment of cancer. Tissue ablation is achieved through the continuous delivery of a narrow range of electromagnetic fields across a tumor, for a period of months. TTFields are designed to affect only cells that divide and to interfere with the ce...
Article
We introduce an isochoric (constant-volume) supercooling cryomicroscope (ISCM), enabling the ice-free study of biological systems and biochemical reactions at subzero temperatures at atmospheric pressure absent ice. This technology draws from thermodynamic findings on the behavior of water in isochoric systems at subfreezing temperatures. A descrip...
Article
Full-text available
Porous dielectric membranes that perform insulator-based dielectrophoresis or electroosmotic pumping are commonly used in microchip technologies. However, there are few fundamental studies on the electrokinetic flow patterns of single microparticles around a single micropore in a thin dielectric film. Such a study would provide fundamental insights...
Article
Full-text available
Low-temperature biopreservation and 3D tissue engineering present two differing routes towards eventual on-demand access to transplantable biologics, but recent advances in both fields present critical new opportunities for crossover between them. In this work, we demonstrate sub-zero centigrade preservation and revival of autonomously beating thre...
Article
An efficient global cold food chain is critical to the sustainability of the growing world population, and it is anticipated that the global frozen food market will reach $404.8 billion by 2027. Frozen foods are typically stored under conventional industry-standard isobaric (constant-pressure) conditions at sub-freezing temperatures, however, which...
Preprint
Full-text available
Stable aqueous supercooling has shown significant potential as a technique for human tissue preservation, food cold storage, conservation biology, and beyond, but its stochastic nature has made its translation outside the laboratory difficult. In this work, we present an isochoric nucleation detection (INDe) platform for automated, high-throughput...
Article
Full-text available
Irreversible electroporation (IRE) is a non-thermal tissue ablative technology that has emerging applications in surgical oncology and regenerative surgery. To advance its therapeutic usefulness, it is important to understand the mechanisms through which IRE induces cell death and the role of the innate immune system in mediating subsequent regener...
Article
There is growing interest in the use of isochoric (constant volume) freezing for cryopreservation of biological matter. The goal of this study is to generate fundamental experimental data on the pressure temperature relation during the freezing of an isochoric system of aqueous solutions of two compounds, glucose and glycerol. Glucose and glycerol...
Article
Full-text available
We present herein an experimental study on the ice-nucleation kinetics of two recently introduced aqueous supercooling modalities—oil-sealed isobaric supercooling and isochoric supercooling. A series of constant-cooling rate experiments compare the apparent nucleation temperatures of pure water supercooled under these modalities with conventional o...
Article
Full-text available
Isochoric freezing is a pressure freezing technique that could be used to retain the beneficial effects of food storage at temperatures below their freezing point without ice damage. In this study, potato cylinders were frozen in an isochoric system and examined using full factorial combinations of three processing procedures (immersed in water, va...
Preprint
Full-text available
Low-temperature ex vivo preservation and tissue engineering based on human induced pluripotent stem cells (hiPSC) represent two of the most promising routes towards on-demand access to organs for transplantation. While these fields are often considered divergent from one another, advances in both fields present critical new opportunities for crosso...
Article
Full-text available
This study investigated the potential of isochoric freezing to preserve tomatoes. Isochoric freezing is an emerging technology that preserves biological matter at subfreezing temperatures without any ice damage. Isochoric freezing was compared with freezing under isobaric conditions and with preservation techniques used in the food industry: cold s...
Article
Full-text available
Objective An experimental study for testing a simple robust algorithm on data derived from an electromagnetic radiation device that can detect small changes in the tissue/fluid ratio in a realistic head configuration. Methods Changes in the scattering parameters (S 21 ) of an inductive coil resulting from injections of chicken blood in the 0–18 ml...
Article
The stability of solid–liquid interfaces during solidification is a physical phenomenon of fundamental interest with a wide range of practical applications, including the freezing of biological matter for medical and agricultural purposes. Much of the classical research in this field treats solidification in isobaric (constant-pressure) systems in...
Article
Isochoric freezing, different from isobaric (conventional) freezing, allows for storage below freezing temperatures without significant damage from ice formation. While several types of tissues have been successfully stored in sub‐zero isochoric conditions, it is unknown how isochoric freezing affects pathogenic microorganisms. Thus, the objective...
Article
The enhanced interest in greater convenience foods has recently led to the expansion of minimally processed potato products. This study investigated the effects of isochoric freezing on pre‐peeled potato cubes, including quality attributes (microstructure, texture, and color), nutritional value (ascorbic acid (AA) content, total phenolic content, a...
Article
Full-text available
The aim of this work was to evaluate the effects of isochoric freezing on the quality of tilapia fillet. Isochoric freezing was compared to chilling, super-chilling, and freezing. Isochoric freezing showed muscle color alterations similar to the other preservation methods. All preservation methods resulted in softer fillets, with the isochoric froz...
Article
Full-text available
Stabilization of supercooled liquids presents a fundamental challenge with broad practical implications. Here, we unify divergent aspects of bubble dynamics, thermodynamics, and nucleation theory to explore the influence of confinement on cavitation-induced nucleation, a principal destabilization mechanism in supercooled systems. We demonstrate tha...
Article
Full-text available
When delivered across a cell, certain pulsed electric fields can cause an increase in the cell membrane permeability through a biophysical process known as electroporation. The current signals during the electric pulses could be used as a method for noninvasive electroporation measurement because of the sharp change in the conductivity of cells due...
Article
This review summarizes the use of high-voltage electrical pulses (HVEPs) in clinical oncology to treat solid tumors with irreversible electroporation (IRE) and electrochemotherapy (ECT). HVEPs increase the membrane permeability of cells, a phenomenon known as electroporation. Unlike alternative ablative therapies, electroporation does not affect th...
Article
Full-text available
Supercooling has recently emerged as a highly promising, multi-scale technique for low-temperature preservation of organs and tissues, preventing damaging ice formation while requiring relatively low doses of added cryoprotectants. However, current supercooling techniques are not thermodynamically stable; mild agitations can cause rapid and destruc...
Article
Full-text available
Water expands upon freezing. What happens when water is cooled below 0 °C in an undeformable, constant-volume container? This is a fundamental question in materials thermodynamics, and is also relevant in biological, geological, and technological applications in which ice forms under nano-, meso-, or macroscale confinement. Here, we analyze the pha...
Article
Three-dimensional (3D) bioprinting is one of the fastest advancing and most promising techniques for tissue engineering. However, despite numerous developments in3D bioprinting, issues of size scalability remain. Two primary factors limiting the scale of printable objects are the structural properties of the bioinks and the un-sustainable time it t...
Article
Full-text available
Background Percutaneous image-guided tissue ablation (IGA) plays a growing role in the clinical management of solid malignancies. Electroporation is used for IGA in several modalities: irreversible electroporation (IRE), and reversible electroporation with chemotoxic drugs, called electrochemotherapy (ECT). It was shown that the combination of elec...
Article
Efforts are currently directed towards improving the quality of vegetables after freezing and thawing. One of the methods under investigation is isochoric freezing. In this study, we evaluated isochoric freezing for preserving the quality of baby‐leaf spinach. We compared the properties of thawed spinach frozen to −4°C in an isochoric system with t...
Article
Hemorrhagic stroke is one of the leading causes of premature death among economically disadvantaged populations. Treatments of these conditions require an early diagnosis. While CT and MRI are the medical gold standard for early diagnosis, these imaging modalities are rarely available in low-and middle-income countries. We present an unsophisticate...
Article
The present study is part of an ongoing effort to develop a simple diagnostic technology for detecting internal bleeding in the brain, which can be used in lieu or in support of medical imaging and thereby reduce the cost of diagnostics in general, and in particular, would make diagnostics accessible to economically disadvantaged populations. The s...
Article
Horizontal directional solidification techniques have been broadly utilized for the freezing of biological matter under conditions in which the freezing rate during solidification must be controlled and known. Directional solidification is used for diverse applications such as fundamental research on freezing of biological materials, cryopreservati...
Article
Full-text available
Freezing of the aqueous solutions that comprise biological materials, such as isotonic physiological saline, results in the formation of ice crystals and the generation of a hypertonic solution, both of which prove deleterious to biological matter. The field of modern cryopreservation, or preservation of biological matter at sub-freezing temperatur...
Article
Irreversible electroporation (IRE), is a new non-thermal tissue ablation technology in which brief high electric field pulses are delivered across the target tissue to induce cell death by irreversible permeabilization of the cell membrane. A deficiency of conventional IRE is that the ablation zone is relatively small, bounded by the irreversible e...
Article
While three-dimensional (3D) printing of biological matter is of increasing interest, current linear 3D printing processes lack the efficiency at scale required to mass manufacture products made of biological matter. This paper introduces a device for a newly developed parallel additive manufacturing technology for production of 3D objects, which a...
Article
Full-text available
Background: The safety and efficacy of irreversible electroporation (IRE) in treating hepatic, biliary, and pancreatic malignancies are active areas of clinical investigation. In addition, recent studies have shown that IRE may enable regenerative surgery and in vivo tissue engineering. To use IRE effectively in these clinical applications, it is...
Article
While biological systems are typically studied under isobaric (constant pressure) conditions, recent reports on the bio-thermodynamics of isochoric (constant volume) systems point to their potential for subfreezing-temperature preservation of biological matter. This preliminary study, in which we report that pancreatic islets can survive multi-day...
Article
Full-text available
Background: Nonthermal irreversible electroporation is a minimally invasive surgery technology that employs high and brief electric fields to ablate undesirable tissues. Nonthermal irreversible electroporation can ablate only cells while preserving intact functional properties of the extracellular structures. Therefore, nonthermal irreversible ele...
Article
Full-text available
A new technique is introduced which addresses the need for faster additive manufacturing methods for tissue scaffolds and frozen foods in large-scale industrial applications, inspired by print lithography. It is particularly relevant to biological matter, which is composed mostly of water. Instead of point-by-point printing in three dimensions (3D)...
Article
In engineering and medicine, there is a growing interest in using textiles made of composites with enhanced thermal properties. One such type of textile is fabric impregnated with ceramics and mineral particles. This material has high emissivity in the infrared range and may have therapeutic benefits for treatments of diseases, like Raynaud's syndr...
Article
Due to the perishable nature of fruits and the importance of reducing food waste, an effective preservation technique is required to prolong the shelf life and maintain the physical and nutritional properties of seasonal fruits. In this study, we evaluated isochoric freezing for preserving the quality of sweet cherries. We examined the physical cha...
Article
Objective: This numerical study was designed to evaluate the feasibility of using an inductive coil for monitoring the changes in the volume of a hematoma in the head in situ and to compare the inductive coil performance to that of a spiral antenna based on the radar principle. Methods: Numerical analysis was used to solve the complete set of Ma...
Article
In comparison with isobaric (constant pressure) freezing, isochoric (constant volume) freezing reduces potential mechanical damage from ice crystals and exposes stored biological matter to a lower extracellular concentration, at the price of increased hydrostatic pressure. This study evaluates the effects of isochoric freezing to low temperatures a...
Article
This study examined the temporal physiological and molecular events following the treatment of the liver with a tissue ablation modality that combined electroporation with electrolysis (E2). Rat liver was treated with an E2 waveform and the tissue examined, 1 h, 3 h, 6 h and 24 h with: H&E, Masson Trichrome, TUNEL stains and Western blot. H&E and T...
Article
Ice-free vitreous cryopreservation (vitrification) is regarded as the principal method for avoiding ice crystallization damage in cryopreserved tissues and organs. We previously established the fundamental thermodynamics of isochoric (constant volume) systems for cryopreservation, and now extend this novel approach to vitrification in an isochoric...
Article
Isochoric (constant volume) freezing has been recently suggested as a new method for cell and organ preservation. As a first step in studying the effect of isochoric freezing on mammalian cells, Madin-Darby canine kidney epithelial cells (MDCK), were frozen in an isochoric system, in a simple extracellular phosphate buffered solution to -10 °C (96....
Article
Full-text available
It was recently shown that electrolysis may play a substantial detrimental role in microfluidic electroporation. To overcome this problem, we have developed a non-electrolytic micro/nano electroporation (NEME) electrode surface, in which the metal electrodes are coated with a dielectric. A COMSOL based numerical scheme was used to simultaneously ca...
Article
Isochoric (constant volume) preservation at subfreezing temperatures is being investigated as a novel method for preserving cells and organs. This study is a first initial effort to evaluate the efficacy of this method for heart preservation, and to provide a preliminary outline of appropriate preservation parameters. To establish a baseline for fu...
Chapter
Full-text available
Electroporation phenomena can be traced back to the eighteenth century when red spots on human animal skin (Lichtenberg figures) were observed in the areas where electric fields were applied. Once the cause of this phenomenon was understood and control over the parameters that produce electric fields was achieved, a quick adoption of the use of pul...
Article
Objective: The goal of this study was to evaluate the technological feasability of a new technology for catheter-based ablation for treatment of AFib that combines freezing with electrolysis. Materials and methods: The study was performed on a pH dye stained gel phantom of the pulmonary vein. Freezing was induced with a cryosurgical probe insert...
Article
Full-text available
Background This theoretical study examines the use of radar to continuously monitor “accumulation of blood in the head” (ACBH) non-invasively and from a distance, after the location of a hematoma or hemorrhage in the brain was initially identified with conventional medical imaging. Current clinical practice is to monitor ABCH with multiple, subsequ...
Data
The change in absolute input impedance for different ratio of signal to noise (SNR). The ordinate is the change in absolute input impedance for different ratio of signal to noise (SNR) at the input to the antenna, at frequencies of 100 MHz, 500 MHz and 1 GHz. Green line is the change in absolute impedance without noise, blue data points include a S...
Data
The difference between the absolute change in impedance with SNR input and without. (TIF)
Data
The change in absolute impedance input for Gaussian noise type error in the electrical parameters. The ordinate is the change in absolute input impedance for Gaussian noise type error in the assumed values of electrical parameters employing 1 GHz. The abscissa is ABCH volume. (TIF)
Chapter
The phenomenon of electroporation can be traced back to the eighteenth century when red spots on skin (Lichtenberg figures) were observed in the areas where electric fields were applied. Once the cause of this occurrence was understood and controlled, induction of electric fields was achieved; the use of pulsed electric fields was adopted in the ar...
Article
Objective Preservation of biological materials at subzero Centigrade temperatures, cryopreservation, is important for the field of tissue engineering and organ transplantation. Our group is studying the use of isochoric (constant volume) systems of aqueous solution for cryopreservation. Previous studies measured the pressure-temperature relations i...
Data
Supporting information data for Fig 4. (XLSX)
Data
Supporting information data for Fig 6. (XLSX)
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Supporting information data for Fig 7. (XLSX)
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Supporting information data for Fig 2. (XLSX)
Data
Supporting information data for Fig 8. (XLSX)
Article
We report results from an acute, single case study in the pig liver on the effects of a tissue ablation protocol (we named cryoelectrolysis) in which 10 min of cryosurgery, with a commercial cryosurgical probe, are delivered after 10 min of electrolysis generated by a current of about 60 mA. The histological appearance of tissue treated with cryoel...
Article
Full-text available
Micro and nano technologies are of increasing importance in microfluidics devices used for electroporation (electroporation – the permeabilization of the cell membrane with brief high electric field pulses). Electrochemical reactions of electrolysis occur whenever an electric current flows between an electrode and an ionic solution. It can have sub...