Boris Jovanović

Boris Jovanović
Iowa State University | ISU · Department of Ecology, Evolution and Organismal Biology

Ph.D.
Microplastics monitoring in freshwater shallow lakes

About

61
Publications
46,069
Reads
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2,239
Citations
Introduction
The main focus of my research is in the area of Nanotoxicology and Aquatic Ecotoxicology. I am interested in studying non-soluble suspended materials of anthropogenic origin - mainly nanoparticles and microplastics and their effects on the environment. My research is a combination of laboratory experimentation, in situ exposure, field studies, and mesocosms.
Additional affiliations
January 2018 - present
Iowa State University
Position
  • Professor (Assistant)
June 2012 - September 2017
Ludwig-Maximilians-University of Munich
Position
  • Group Leader
January 2008 - June 2012
Iowa State University
Position
  • Research Assistant

Publications

Publications (61)
Article
Full-text available
From 1916-2011, an estimated total of 165,050,000 metric tonnes of titanium dioxide pigment were produced worldwide. Current safety regulations on the usage of the TiO2 pigment as an inactive ingredient additive in human food are based on legislation from 1969 and are arguably outdated. This paper compiles new research results to provide fresh data...
Article
Full-text available
Over the course of 78 days, 9 outdoor mesocosms, each with 1350 L capacity, were situated on a pontoon platform in the middle of a lake and exposed to 0 µg L−1 TiO2, 25 µg L−1 TiO2, or 250 µg L−1 TiO2 nanoparticles in the form of E171 TiO2 human food additive five times a week. Mesocosms were inoculated with sediment, phytoplankton, zooplankton, ma...
Article
The ubiquitous presence of microplastics (MP) in aquatic ecosystems can affect organisms and communities in multiple ways. While MP research on aquatic organisms has primarily focused on marine ecosystems and laboratory experiments, the community-level effects of MP in freshwaters, especially in lakes, are poorly understood. To examine the impact o...
Article
Full-text available
The European Marine Strategy Framework Directive and the United States Microbead Free Waters Act are credited for being ambitious in their goals for protecting the marine environment from microplastics pollution. As a result, the microplastic pollution of marine environments and the incidence of microplastic ingestion by fish is rapidly receiving a...
Article
As the levels of plastic use in global society have increased, it has become crucial to develop statute, regulation, and policy to regulate plastics of all sizes including both microplastics and nanoplastics (MPs/NPs). In this paper, the published literature on the current laws passed by Congress and regulations developed by various federal agencie...
Chapter
Open educational resources (OER) offer opportunities for pedagogical change through participatory practices in higher education. Open pedagogy emerged as a teaching practice to empower learners in the creation of OER content. This chapter presents three cases where open pedagogy was put into practice in a higher education institution. The three cas...
Article
Benthic communities contain some of the most threatened organisms in aquatic habitats due to different anthropogenic pressures. The high abundance of microplastics (MPs) in sediments will continue to increase in the future, further increasing the probability of interactions between macroinvertebrates and MPs. In the present study, a benthic communi...
Article
Due to their many practical applications, engineered cerium dioxide (CeO2) nanoparticles (NPs) are commonly used today, although there are justified concerns about the environmental risks of their usage. Studies to date have shown that CeO2 NPs do not pose a lethal hazard to hydrobiocenoses at environmentally relevant concentrations, but that they...
Article
Identifying leading publications, authors, and countries in microplastic and nanoplastic research is beneficial for regulatory decisions, determining standardized research methodology, and solidifying definitions. Here, bibliometric analysis was performed using Web of Science's Core Collection to evaluate publication trends. A total of 3820 publica...
Conference Paper
Full-text available
Present within the OECD priority list for safety testing of manufactured nanomaterials, cerium oxide nanoparticles (nano-CeO2), present one of today's’ major contaminants of the environment. The present study was carried out in order to explore the potential of the geometric morphometric approach in unraveling sublethal implications of nano-CeO2 ex...
Conference Paper
Full-text available
The conference paper presents the effects of microplastic mixture on community structure of freshwater benthic macroinvertebrates in-situ. Research was conducted in microcosm setup, under natural conditions and lasted for 100 days.
Article
Drosophila is among the most commonly use models for toxicity assessment of different types of nanoparticles. This study aims to examine the effects of a constant exposure to the low concentration of human food grade titanium dioxide nanoparticles (TiO2 E171) on Drosophila melanogaster wing morphology over multiple generations. Subsequently, the Ge...
Article
Full-text available
Petasites hybridus (Common butterbur) is extensively used in traditional medicine, and is currently gaining interest and popularity as a food supplement and for its medicinal properties. It contains a large number of active compounds of potential therapeutic activity, but also toxic pyrrolizidine alkaloids. Science-based information is needed to su...
Article
Cyanobacteria and microalgae are abundant biota groups in eutrophic freshwater ecosystems, serving as a food source for many aquatic organisms, including the larvae of non-biting midges (Chironomidae). Many species of cyanobacteria are toxin producers, which can act as stressors to other organisms. The present study aimed to analyze and compare the...
Article
The effect of microplastics (MP) exposure on the chironomid species Chironomus riparius Meigen, 1804 was investigated using the OECD sediment and water toxicity test. Chironomid larvae were exposed to an environmentally relevant low microplastics concentration (LC), a high microplastics concentration (HC) and a control (C). The LC was 0.007 g m-2 o...
Conference Paper
Full-text available
The potential of two chironomid species: Chironmus riparius and C. tentans in bioindication with the focus on relevant endpoints in assessing nanoparticle ecotoxicity in fresh waters.
Article
Cerium oxide nanoparticles (nano‐CeO2) are included in the OECD priority list of engineered nanomaterials for the assessment of their environmental impact. The present study was carried out in order to assess the nano‐CeO2 toxicity to freshwater midge Chironomus riparius larvae at concentrations of 2.5, 25, 250 and 2500 mg of nano‐CeO2 per kg of se...
Experiment Findings
https://en.wikibooks.org/wiki/Perspectives_of_Aquatic_Toxicology
Conference Paper
Microplastics (MPs) are ubiquitous pollutants found in marine, freshwater, and terrestrial ecosystems, and MP pollution is a major environmental concern globally. Despite this, the impact of MPs on freshwater ecosystems is largely under-looked, with most research concentrating on marine systems and organisms. Owing to their small and wide range of...
Conference Paper
Full-text available
This conference paper presents our research on CeO2 nanoparticle toxicity to freshwater midge C. tentans and points out relevant endpoints that are sensitive detectors of nano-CeO2 presence in the sediments.
Conference Paper
Full-text available
The conference paper presents the effects of different types of microplastic polymers on Chironomus riparius, regarding the life traits and morphological changes in the mandibles and mentums of 4th instar larvae and morphological changes in the wings of adults.
Article
The release of nanoparticles to the environment can affect health of the exposed organisms. MicroRNAs have been suggested as potential toxicology biomarkers, however the information about use of microRNA in aquatic organisms exposed to nanoparticles (NP) is limited. In silico analysis from publicly available gene expression data was performed. Data...
Poster
Full-text available
This research was conducted in order to determine influence of mixture of microplastic particles (MP) on non-biting midges Chironomus spp. in laboratory and mesocosm setup.
Article
Full-text available
Abstract In this study, fruit flies (Drosophila melanogaster) were exposed to an estimated daily human E171 consumption concentration for 20 generations. Exposure to E171 resulted in: a change in normal developmental and reproductive dynamics, reduced fecundity after repetitive breeding, increased genotoxicity, the appearance of aberrant phenotypes...
Article
The present study was carried out in order to assess the influence of environmentally relevant concentrations of TiO2 nanoparticles (E171 human food grade) toxicity on the freshwater midge Chironomus tentans. Tested concentrations were 125, 250, 500, 1000, 2000 and 4000 mg of E171 TiO2 per 1 kg of sediment, for the experiment aiming at life trait t...
Poster
Full-text available
- Chironomus tentans was exposed to food grade E171 TiO2 nanoparticles - Mouthpart deformities of chironomids were analyzed with geometric morphometry - Dietary exposure to TiO2 caused losing the first inner tooth in the mandibles - Mortality and emergence ratio were affected with higher concentrations (>1000 mg/kg)
Article
Among aquatic organisms, fish are particularly susceptible to ingesting microplastic particles due to their attractive coloration, buoyancy, and resemblance to food. However, in previous experimental setups, fish were usually exposed to unrealistically high concentrations of microplastics, or the microplastics were deliberately contaminated with pe...
Article
Comparative analysis of the chemical composition and biological activity of the essential oils from the needles of four Pinus species (P. mugo subsp. mugo, P. nigra subsp. nigra, P. sylvestris and P. peuce) from the central Balkans was performed. Although different chemical profiles of the dominant terpenes for each species were determined, essenti...
Chapter
Nanoparticles have been present on our planet for billions of years. While naturally occurring nanoparticles are nothing new, man-made nanoparticles are of much more recent history. At the moment the yearly production quantity of nanomaterials is largely underestimated, as production of incidental nanoparticles is not being monitored although many...
Conference Paper
Full-text available
The effect on mouthpart morphological changes was investigated by the geometric morphometric approach.
Article
Water pollution with large and small-scale plastic litter is an area of growing concern. Macro plastic litter is a well-known threat to aquatic wildlife; however, effects of micro and nano-sized plastic particles on the health of organisms are not well understood. Small scale plastic particles can easily be ingested by various aquatic organisms and...
Thesis
Full-text available
Titanium dioxide nanoparticles (TiO2) have the potential to cause adverse effects on the health of aquatic animals and humans, but the understanding of the underlying mechanisms is limited. Major task of this thesis was to connect gaps in the current knowledge of TiO2 toxicology and mode of action with a comprehensive research including molecular,...
Article
Full-text available
Dimethyl sulfoxide (DMSO) is commonly used as a solvent for organic compounds but its toxic properties can affect both in vitro and in vivo studies. Thus, evaluation of DMSO toxicity must be performed on the model organism before its application as a solvent in the target studies. Present study aimed to determine the lethal concentration required t...
Technical Report
Full-text available
The aim of the present study was to reconstruct total Russian fisheries catch in the Black Sea and Sea of Azov for the period 1950 to 2010. Using catches presented by FAO on behalf of the USSR and Russian Federation as a baseline, total removals were estimated by adding estimates of unreported commercial catches, discards at sea, and unreported rec...
Article
Full-text available
Nano-TiO2 is immunotoxic to fish and reduces the bactericidal function of fish neutrophils. Here, fathead minnows (Pimephales promelas) were exposed to low and high environmentally relevant concentration of nano-TiO2 (2 ng g(-1) and 10 μg g(-1) body weight, respectively), and were challenged with common fish bacterial pathogens, Aeromonas hydrophil...
Article
The aim of the present study was to evaluate the nano-TiO2 toxicity to zebrafish embryos through evaluating the success in hatching in relationship with hours post-exposure instead of considering just the total hatching rate. Zebrafish embryos 4h post-fertilization were exposed to nTiO2 (0, 0.01, 10, and 1000µgmL(-1)) for 130h. The hatching rate (H...
Article
Full-text available
Titanium dioxide nanoparticles are photoactive and under natural sunlight, produces reactive oxygen species. Reactive oxygen species can be detrimental to many organisms, causing oxidative damage, cell injury, and death. Most studies investigating TiO2 nanoparticle toxicity did not consider photoactivation and performed tests either in dark conditi...
Article
Full-text available
Abstract Hydroxylated fullerenes are reported to be very strong antioxidants, acting to quench reactive oxygen species (ROS), thus having strong potential for important and widespread applications in innovative therapies for a variety of disease processes. However, their potential for toxicological side effects is still largely controversial and un...
Article
In this paper, the chemical composition and biological activity of the essential oil of Artemisia absinthium was studied. The aim of this study was to investigate the potential of ethnopharmacological uses of this plant species in the treatment of gastrointestinal diseases and wounds, and as an insect repellent. The aerial part of the plant was hyd...
Article
Full-text available
The increase in the use of manufactured titanium dioxide nanoparticles (nano-TiO2 ) is causing the rise of their concentration in the aquatic environment, including coral reef ecosystems. Caribbean mountainous star coral (Montastraea faveolata) has frequently been used as model species to study gene expression during stress and bleaching events. Th...
Article
Full-text available
The rapid increase in use of nanotechnology products is increasing the presence of metal, metal-oxide and carbon-based nanoparticles in the aquatic environment. These non-functionalized engineered nanoparticles can interact with the immune system of fish and invertebrates, and tip the ecological balance of population sustainability. Most nanopartic...
Article
Full-text available
Variation of 22 morphometric and 4 meristic characters of the species Alburnoides bipuuctatus from Nišava River were analyzed. Specimens were collected from four sampling stations along the putative mesohabitat gradient of river influence and were grouped into the categories with similar total length. Morphometric characters varied immensely betwee...
Article
Full-text available
The Nišava River plays an important role as the source for both drinking water and agricultural irrigation due to its hydrological and geomorphological characteristics as the largest river in the region of southeast Serbia. In this study we used the liver of the chub (Leiciscus cephalus) as a tool for biomonitoring heavy metal accumulation along th...
Thesis
Full-text available
Nanoparticles have the potential to cause adverse effects on the fish health, but the understanding of the underlying mechanisms is limited. Major task of this dissertation was to connect gaps in current knowledge with a comprehensive sequence of molecular, cellular and organismal responses toward environmentally relevant concentrations of engineer...
Article
Increased release of engineered nanoparticles to the environment suggests a rising need for the monitoring and evaluation of potential toxicity. Zebrafish frequently have been used as a model species in human and aquatic toxicology studies. In this study, zebrafish embryos were microinjected in the otic vesicle with a sublethal dose of engineered n...
Article
Fathead minnows Pimephales promelas were exposed to lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid [poly(I:C)] to observe immunological responses during simulated bacterial and viral challenge at the level of gene expression and granulocyte function. Complementary DNA libraries were created from LPS- and poly(I:C)-treated fish and c....
Article
Hydroxylated fullerenes act as potent inhibitors of cytochrome P450-dependent monooxygenases, and are reported to be very strong antioxidants quenching reactive oxygen species (ROS) production. Effects of nanosized hydroxylated fullerenes on fish neutrophil function and immune gene transcription was investigated using fathead minnow (Pimephales pro...
Article
Fathead minnows Pimephales promelas were exposed to lipopolysaccharide (LPS) and polyinosinicpolycytidylic acid [poly(I:C)] to observe immunological responses during simulated bacterial and viral challenge at the level of gene expression and granulocyte function. Complementary DNA libraries were created from LPS- and poly(I:C)-treated fish and c. 5...
Article
Effects of nanosized (<100 nm) titanium dioxide (TiO(2)) particles on fish neutrophils and immune gene expression was investigated using the fathead minnow (Pimpehales promelas). Expanded use of TiO(2) in the cosmetic industry has increased the potential exposure risk to aquatic ecosystems and human health. Effects of nano-TiO(2) on neutrophil func...
Article
Estuaries, lagoons and mudflat habitats represent a crucial biodiversity component in coastal waters and may act as vital nurseries and shelters for the juvenile stages of many commercial fish species. A study of the spatial distribution of inshore fish fauna around the shallow coastal habitats of North Bull Island (Dublin Bay, Ireland) was carried...

Questions

Question (1)
Question
I am not a fruit fly person (rather a fish guy) but am designing a toxicology experiment with Drosophila, and I need to know how much food medium does Drosophila larvae eats per day? Are there any estimates - I would appreciate a link to the reference.
P.S. I do not need the estimate on the adult flies, just on the larvae.

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Projects

Projects (4)
Project
Current scientific research on microplastics is predominantly focused on marine environment and on individual species, while freshwater studies, including mesocosm studies, are lacking significantly. MICROFREAK aimed to perform world first freshwater, environmentally realistic, outdoor mesocosm microplastics study. Objectives of MICROFREAK were to follow changes in the biomass across trophic levels; follow the trophic transfer of microplastics; follow potential migration of microplastics from aquatic to terrestrial environment through insect vectors; determine changes in the community structure; and follow life cycle and deformities in chironomid larvae and adults as sublethal implications of microplastics exposure. The main goal of this study is to evaluate effects of microplastics (if any) in freshwater ecosystem by exposing water and sediment of mesocosms simultaneously to the environmentally relevant mixture of various microplastic polymers and not to individual microplastic types. Performing a mesocosm study with a mixture of microplastic is very important as in the nature we do not have freshwater systems that are contaminated only with a single plastic polymer. Different plastics types have different densities resulting in a different distribution patterns in the aquatic ecosystem while many aquatic organisms move/migrate throughout the day and may interact with different types of plastics from different compartments of the ecosystem. Thus, in order to truly understand the effects of microplastics on freshwater ecosystem we performed an outdoor mesocosm experiment with an environmentally relevant concentration of microplastics mixture and spiked water surface, water column, and sediment with microplastics by using different plastic densities Stay tuned for updates.
Project
Open Pedagogy emerges as a teaching approach that empowers learners in the co-creation of the course content and contributing the body of knowledge from which they are learning . This project aims to investigate the impact of the integration of open pedagogy assignments and activities into higher education classrooms. Example in progress: https://en.wikibooks.org/wiki/Perspectives_of_Aquatic_Toxicology