Bohdan O Manko

Ivan Franko National University of Lviv | ivan franko · Department of Human and Animal Physiology

The exocrine function of pancreatic acini cells is a highly energy-intensive process. A cell always needs to maintain a stable level of ATP balancing between states of activation and rest. Low-energy is one of the possible mechanisms contributing to the development of pancreatic diseases. The most commonly encountered disease of the pancreas is acu...

Chronic alcohol consumption may cause pancreatitis and alcohol-related liver diseases. Both adaptation and damage of liver mitochondria in animals on chronic ethanol and high-fat diets were demonstrated. It is currently not clear if ethanol or its metabolites such as fatty acid ethyl esters can cause mitochondrial damage to the pancreas. The presen...

Background. Mitochondria can influence cancer cells both indirectly via reactive oxygen species mediation and directly through mitochondrial biogenesis. Energy production in mitochondria is crucial as it facilitates the synthesis of essential molecules needed for cellular biosynthesis, growth, and proliferation. The development of new anticancer dr...

Відомо , що етанол впливає на окисні процеси у мітохондріях печінки, а це може бути одним із механізмів розвитку алкогольної хвороби печінки. Відомий імуносупресант циклоспорин А також є інгібітором мітохондріальної пори перехідної провідності. Однак фармакологічна взаємодія етанолу та циклоспорину А недостатньо вивчена. Мета цього дослідження – до...

Objectives Amino acids play an essential role in protein synthesis, metabolism and survival of pancreatic acini. Adequate nutritional support is important for acute pancreatitis treatment. However, high concentrations of arginine and lysine may induce acute pancreatitis. The study aimed to identify the most suitable L-amino acids as safe energy sou...

An unhealthy diet often is a cause of obesity, chronic inflammation, and metabolic disruption in multiple organs. However, the direct influence of elevated lipid or sugar consumption on liver, pancreatic, and sperm mitochondria is not well understood. The aim of the study was to investigate the functional activity of mitochondria of liver, pancreat...

Plasma amino acid levels are altered upon many pathological conditions including acute pancreatitis. It is unclear whether amino acids can be used as specific biomarker of acute pancreatitis severity or recovery. Development of acute pancreatitis is associated with mitochondrial dysfunction and decreased cytosolic ATP level. Sodium pyruvate is cons...

Background. Assessment of cell viability is crucial in cell studies. Testing plasma membrane integrity is a traditional approach of evaluating cell viability. Mitochondrial functional capacity closely correlates with plasma membrane integrity and overall cell health. This study aimed to investigate whether any aspect of mitochondrial adaptive capac...

Amino acids play an essential role in protein synthesis, metabolism and survival of pancreatic acinar cells. Adequate nutritional support is important for acute pancreatitis treatment. However, some amino acids, such as arginine and lysine, are toxic for pancreatic acinar cells in high concentrations. The study aimed to select the candidate amino a...

The development of a new anticancer drugs targeted at energy metabolism of tumor cells is a promising­ approach for cancer treatment. The aim of our study was to investigate the action of thiazole derivative N-(5-benzyl-1,3-thiazol-2-yl)-3,5-dimethyl-1-benzofuran-2-carboxamide (BF1) and its complex with PEG based polymeric nanoparticle (PEG-PN) on...

Plasma amino acid levels are altered upon many pathological conditions including acute pancreatitis. It is unclear whether amino acids can be used as specific biomarker of acute pancreatitis severity or recovery. Development of acute pancreatitis is associated with mitochondrial dysfunction and decreased cytosolic ATP level. Sodium pyruvate is cons...

BACKGROUND-AIM A piece of evidence suggests that male obesity negatively affects sperm quality. Presently, the increased sugar consumption is considered the main cause of obesity. The results of few studies indicate that a high-fat diet has a detrimental effect on sperm mitochondrial respiration, yet the effects of additional sugar supplementation...

Objectives: The objective of this study was to test whether pyruvate and glutamine affect the ethanol and cholecystokinin (CCK) effects on the mitochondrial function, viability, and morphology of rat pancreatic acini. Methods: Respiration was measured with Clark oxygen electrode. Mitochondrial membrane potential, reduced nicotinamide adenine din...

During glutamine catabolism is produced ammonia, which can be toxic to cells. In hepatic encephalopathy neuron mitochondria ammonia causes the formation of free radicals, the opening of the mitochondrial permeability transition pore, oxidative phosphorylation disruption and swelling. It is still unknown whether the utilization of glutamine in the m...

Insulin increases the basal and agonist-stimulated secretion of pancreatic acinar cells, which leads to increase of energy demand and requires sufficient oxidative substrates supply. Cholecystokinin substantially increases the respiration rate of pancreatic acinar cells upon pyruvate oxidation. However, it is not clear how insulin affects mitochond...

When conducting studies on isolated hepatocytes, it is important to obtain cells that retain the functional properties that are characteristic of the whole organ. Increased blood viscosity during liver perfusion, decreased perfusion pressure in blood vessels, and hence hypoxia, are among the factors that may affect the functional state of isolated...

Mitochondrial Ca2+ overload may trigger the opening of mitochondrial permeability transition pore (mPTP) and its prolonged activation leads to cell death. ATP synthase is considered as a possible molecular component of the pore. The aim of this study was to investigate the state of oxidative phosphorylation at Ca2+-induced activation of mPTP in per...

The ability of the mitochondria to compensate for energy expenditure of cells largely depends on the availability of the oxidative substrates, transported across the intact plasma membrane with molecular carriers of limited affinity. The aim of this study was to investigate the dependence of adaptive respiratory responses of mitochondria of intact...

The dependence of uncoupled respiratory capacity of intact pancreatic acini on oxidative substrate supply and functional cell state has not yet been studied in detail. In this study, the respiratory responses of isolated pancreatic acini to FCCP were measured with Clark electrode and mitochondrial membrane potential was assessed with rhodamine123 f...

To verify experimentally the model of permeabilized hepatocytes, the degree of cell permeability was assessed using trypan blue and polarographycally determined cell respiration rate upon succinate (0.35 mM) and α-ketoglutarate (1 mM) oxidation. Oxidative phosphorylation was stimulated by ADP (750 μM). Hepatocyte permeabilization depends on digiton...

The dependence of respiration rate of rat permeabilized acinar pancreacytes on oxidative substrates concentration was studied at various [Ca2+] - 10-8-10-6 M. Pancreacytes were permeabilized with 50 microg of digitonin per 1 million cells. Respiration rate was measured polarographically using the Clark electrode at oxidation of succinate or pyruvat...

The influence ofadenosine diphosphate (ADP) on respiration of pancreatic acinar cell mitochondria in situ was studied. The model of digitonin-treated pancreatic acini was used. It was found that succinate or a mixture of pyruvate, glutamate and malate intensified respiration ofpermeabilized cells. Low ADP concentration (100 microM) did not influenc...

The influence of adenosine diphosphate (ADP) on respiration of pancreatic acinar cell mitochondria in situ was studied. The model of digitonin-treated pancreatic acini was used. It was found that succinate or a mixture of pyruvate, glutamate and malate intensified respiration of permeabilized cells. Low ADP concentration (100 μM) did not influence...

Investigation of the relationship between the processes of Ca2+-signaling and mitochondrial oxidation of cells is an important problem of modern physiology. The reason for this is that the violation of these interdependencies might be the cause of various diseases, including acute pancreatitis. However, dependence of the rate of mitochondrial respi...

The dependence of respiration rate of rat permeabilized acinar pancreacytes on oxidative substrates concentration was studied at various [Ca2+] - 10-8-10-6 M. Pancreacytes were permeabilized with 50 microg of digitonin per 1 million cells. Respiration rate was measured polarographically using the Clark electrode at oxidation of succinate or pyruvat...

Aim: Acetylcholine as one of the main secretagogues modulates mitochondrial functions in acinar pancreacytes, presumably due to increase in ATP hydrolysis or Ca(2+) transport into mitochondria. The aim of this work was to investigate the mechanisms of carbachol (CCh) action on respiration and oxidative phosphorylation of isolated pancreatic acini....

Dependence of respiration of isolated rat pancreatic acini on conditions of their suspension stirring in the polarographic cell was investigated using Clark electrode. The suspension of isolated acini was obtained using collagenase. Cells within isolated acini retained the structural and functional intactness after isolation: 0.1% solution of trypa...

Mitochondria maintain numerous energy-consuming processes in pancreatic acinar cells, yet characteristics of pancreatic mitochondrial oxidative phosphorylation in native conditions are poorly studied. Besides, it is not known which type of solution is most adequate to preserve functions of pancreatic mitochondria in situ. Here we propose a novel ex...

In salivary glands secretory cells of Chironomus plumosus larvae IP3Rs, RyRs and SERCA are components of endoplasmic Ca2+-functional unit (Manko, 2008; 2011). According to the results of our previous studies (Velykopolska, Manko, 2009) activation of P2Y-receptors of the investigated cells leads to activation of the unit, causing release of Ca2+ fro...

The relationship between cellular metabolism and signalling is an important problem in physiology. Acetylcholine as one of the main secretagogues modulates mitochondrial functions in acinar pancreacytes (Voronina et al., 2002; 2004; 2010), presumably due to increase of ATP hydrolysis or Ca2+-transport into mitochondria. The aim of this work was to...

Using Clark oxygen electrode, dependence of mitochondrial functions on Ca(2+)-release channels activity of Chironomus plumosus L. larvae salivary glands suspension was investigated. Cells were ATP-permeabilized in order to enable penetration of exogenous oxidative substrates. Activation of plasmalemmal P2X-receptors (as well as P2Y-receptors) per s...

Respiration characteristics of mitochondria of the parental and giant cells of murine NK/Ly (Nemeth-Kellner lymphoma) were studied. The giant cell-enriched ascites were obtained by serial intraperitoneal injections of vinblastine in tumour-bearing mice. Ascites containing >70% giant cells were used. Their diameter of was over 17 μm (~2800 μm(3)), w...

Insulin effect on energy supplying processes in isolated rat hepatocytes at different duration of hormone action at succinate and a-ketoglutarate oxidation was investigated. Short-term insulin action in vitro does not influence the examined processes. In vivo insulin effect is characterized by time dependence with activation of ADP-stimulated succi...

In the last decade there has been a significant increase of articles related to giant cell transformation in different cancers. But it's still unknown what mechanisms are involved in such giant cancer cells. After vinblastine treatment NK/Ly lymphoma cells undergo giant cell transformation with several cytoskeletal and membrane changes. That's why...

It's known that vinblastine can cause giant cell transformation. In the last decade there has been a significant increase in articles related to giant cell transformation in cancer cells, and murine NK/Ly lymphoma cells undergo such transformation and can form giant cells after vinblastine treatment (Lootsik MD, Stoika RS and others). In 1924 Otto...

In this rewiew we discuss present concepts of functioning of Ca2+-transporting systems in se-cretory cells of exocrine glands. In details are described voltage-operated, receptor-operated and store-operated Ca2+-channals of plasma membrane, ryanodine receptors, inositol-1,4,5-receptors of endoplasmic reticulum, Ca2+-pumps of plasma membrane and end...