Bogdan V. Parakhonskiy

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• PhD
• Scientist at Ghent University

Mass and heat energy transfer are critical for crystallization, particularly for unstable polymorphs like calcium carbonate. This study explores how different energy transfer methods affect the rapid synthesis of calcium carbonate particles, emphasizing heat and mass transfer, nucleation site formation, and crystal growth. Methods investigated in t...

Image segmentation is one of the typical computer vision tasks that has received great success with the recent advance of deep-learning methods. However, it is still a challenging problem, particularly when encountering limited data. In this paper, we present a new strategy for particle image segmentation, which relies on extensive data augmentatio...

Particle assembly is a promising technique to create functional materials and devices from nanoscale building blocks. However, the control of particle arrangement and orientation is challenging and requires careful design of the assembly methods and conditions. In this study, the static and dynamic methods of particle assembly are reviewed, focusin...

Nitrogen source–modified biochar was produced from rice straw–derived biochar (RSBC) via pyrolysis carbonization using nitrogen sources such as ammonium chloride, dicyandiamide, melamine, thiourea, and urea. The characteristics of the resulting biochars were compared, including their elemental compositions, surface functional groups, and morphology...

Biomaterials composed of food polysaccharides are of great interest for future biomedical applications due to their great biocompatibility, tunable mechanical properties, and complex architectural designs that play a crucial role in the modulation of cell adhesion and proliferation. In this work, a facile approach was designed to obtain novel 3D al...

The importance of the clearance of dead cells has been shown to have a regulatory role for normal tissue homeostasis and for the modulation of immune responses. However, how mechanobiological properties of dead cells affect efferocytosis remains largely unknown. Here, we report that the Young's modulus of cancer cells undergoing ferroptosis is redu...

Studying microplastics and nanoplastics (MNP) in environmental matrices is extremely challenging, and recent developments in labelling techniques may hold much promise to further our knowledge in this field. Here, we reviewed MNP labelling techniques and applications to provide the first systematic and in-depth insight into MNP labelling. We classi...

Magnetic systems have always been considered as attractive due to their remarkable versatility [...]

Hybrid materials or hybrids incorporating organic and inorganic constituents are emerging as a very potent and promising class of materials due to the diverse but complementary nature of their properties. This complementarity leads to a perfect synergy of properties of the desired materials and products as well as to an extensive range of their app...

This is the first comprehensive study of the impact of biodegradation on the structure, surface potential, mechanical and piezoelectric properties of poly(3‐hydroxybutyrate) (PHB) scaffolds supplemented with reduced graphene oxide (rGO) as well as cell behavior under static and dynamic mechanical conditions. There is no effect of the rGO addition u...

Transferring the layer-by-layer (LbL) coating approach from planar surfaces to spherical templates and subsequently dissolving these templates leads to the fabrication of polyelectrolyte multilayer capsules. The versatility of the coatings of capsules and their flexibility upon bringing in virtually any material into the coatings has quickly drawn...

Nanoarchitectonics, like architectonics, allows the design and building of structures, but at the nanoscale. Unlike those in architectonics, and even macro-, micro-, and atomic-scale architectonics, the assembled structures at the nanoscale do not always follow the projected design. In fact, they do follow the projected design but only for self-ass...

Being extra strong but super easy to break – entities with such properties would be desired for many applications, particularly in drug delivery, where polyelectrolyte multilayer capsules took a prominent place due to applications in catalysis, intracellular delivery, and carriers of biomolecules and enzymes. Assembly of such capsules has been typi...

Polymeric microcapsules composed by the layer-by-layer (LbL) approach have been used for various applications including drug delivery into cells and in vivo, conducting enzyme catalyzed reactions, performing sensoric functions. Typically, LbL-assembled microcapsules have been formulated via alternating deposition of positively and negatively charge...

To mitigate the hindered transport of multifunctional diagnostics and therapeutics nanoagents in the tumour tissue, we introduce a new drug delivery concept, which relies on drug container passive accumulation in the tumour vasculature followed by a rapid release of small-molecule payload that penetrates through capillary walls to the tumour inters...

Targeted delivery of pharmaceuticals is promising for efficient disease treatment and reduction in adverse effects. Nano or microstructured magnetic materials with strong magnetic momentum can be noninvasively controlled via magnetic forces within living beings. These magnetic carriers open perspectives in controlling the delivery of different type...

Cancer is a serious health problem mainly characterized by unregulated cell divisions. It is known that malign cells display cancer-specific glycans that can be recognized by lectins, proteins with capacity of specific binding to carbohydrates. One such protein is the Dioclea violacea lectin (DVL), a known antiproliferative lectin that has been app...

Research on microplastics (MP) in soils is much complicated due to the lack of dedicated (extraction) methodologies and the strong matrix interferences for MP detection, and there is almost no research on the dynamics of the smallest MP in soil. Here we compared the possible detection of the smallest MP fraction (1-2 µm) by µ-Raman spectroscopy and...

Because free therapeutic drug molecules often have adverse effects on normal tissues, deliver scanty drug concentrations and exhibit a potentially low efficacy at pathological sites, various drug carriers have been developed for preclinical and clinical trials. Their physicochemical and toxicological properties are the subject of extensive research...

Hydrogels, which are versatile three-dimensional structures containing polymers and water, are very attractive for use in biomedical fields, but they suffer from rather weak mechanical properties. In this regard, biocompatible particles can be used to enhance their mechanical properties. The possibility of loading such particles with drugs (e.g. en...

Being extra strong but super easy to break – entities with such properties would be desired for many applications, particularly in drug delivery, where polyelectrolyte multilayer capsules took a prominent place due to applications in catalysis, intracellular delivery, and carriers of biomolecules and enzymes. Assembly of such capsules has been typi...

To mitigate side effects in systemic administration, anticancer drugs are encapuslated in nanocontainers. The nanocontainers are impermeable through normal vessel walls but can permeate and retain in the tumor, albeit their diffusive transport in the tumor interstitium towards pharmacological targets is drastically hindered by the tumor microenviro...

In current orthopedic practice, bone implants used to-date often exhibit poor osteointegration, impaired osteogenesis, and, eventually, implant failure. Actively pursued strategies for tissue engineering could overcome these shortcomings by developing new hybrid materials with bioinspired structure and enhanced regenerative potential. In this study...

To develop materials for drug delivery and tissue engineering and to study their efficiency with respect to ossification, it is necessary to apply physicochemical and biological analyses. The major challenge is labor-intensive data mining during synthesis and the reproducibility of the obtained data. In this work, we investigated the influence of t...

Encapsulation of cells has been an active area of research. Among various methods for encapsulation, Layer-by-Layer (LbL) offers extensive flexibility in the design of surfaces and their interfacial nanoarchitectonics. A diverse range of functionalities have been recently demonstrated for cell encapsulation including protection and improved circula...

Ribosome inactivating proteins (RIPs) are capable of removing a specific adenine from 28S ribosomal RNA, thus inhibiting protein biosynthesis in an irreversible manner. In this study, recombinant OsRIP1, a type 1 RIP from rice (Oryza sativa L.), was investigated for its anti-proliferative properties. Human cervical cancer HeLa cells were incubated...

Nanofibrillar Hydrogels for Cell Growth In article number 2002202, Andre G. Skirtach and co-workers develop nanofibrillar hydrogels by a new and simple self-assembly procedure upon thermal annealing that is shown to be an effective cell growth platform. Biological and medical implications of nanofibrillar structures are discussed. Developing nanofi...

Piezoelectricity is considered one of the key functionalities in biomaterials to boost bone tissue regeneration, however, integrating biocompatibility, biodegradability and 3D structure with pronounced piezoresponse remains a material challenge. Herein, novel hybrid biocompatible 3D scaffolds based on biodegradable poly(3-hydroxybutyrate) (PHB) and...

Nanofibrillar structures are of importance in biomedicine, including lung, cardiovascular, liver, skin, neuroscience research, and tissue engineering. Developing advanced materials and interfaces should contribute to uncovering the mechanisms of diseases aiming to find cure. The similarity between the extracellular matrix (ECM) of soft tissue and h...

Carbone nanotubes (CNTs) possess distinct properties, for example, hardness, which is very complementary to biologically relevant soft polymeric and protein materials. Combining CNTs with bio-interfaces leads to obtaining new materials with advanced properties. In this work, we have designed novel organic-inorganic hybrid coatings by combining CNTs...

As the next generation of materials for bone reconstruction, we propose a multifunctional bioactive platform based on biodegradable piezoelectric polyhydroxybutyrate (PHB) fibrous scaffolds for tissue engineering with drug delivery capabilities. To use the entire surface area for local drug delivery, the scaffold surface was uniformly biomineralize...

Originally regarded as auxiliary additives, nanoparticles have become important constituents of polyelectrolyte multilayers. They represent the key components to enhance mechanical properties, enable activation by laser light or ultrasound, construct anisotropic and multicompartment structures, and facilitate the development of novel sensors and mo...

While DNA and messenger RNA (mRNA) based therapies are currently changing the biomedical field, the delivery of genetic materials remains the key problem preventing the wide introduction of these methods into clinical practice. Therefore, the creation of new methods for intracellular gene delivery, particularly to hard-to-transfect, clinically rele...

Biodegradable piezoelectric 3D polymer scaffolds attract great attention due to possibilities to mimic the functional and mechanical properties of the extracellular matrix, avoiding secondary surgery, and electrically stimulated tissue repair. However, the preservation of piezoelectric response and improvement of wettability of hydrophobic fibrous...

The possibilities created by tuning the architecture of hydrogels open up extensive opportunities for their application in tissue engineering and biomedicine. A simple and effective procedure is developed here, based on thermal annealing, which affects the alignment of the fibrils and results in a mesh‐like structure. Atomic force microscopy and cr...

Developing materials for tissue engineering and studying the mechanisms of cell adhesion is a complex and multifactor pro-cess that needs analysis using physical chemistry and biology. The major challenge is the labor-intensive data mining as well as requirements of the number of advanced techniques. For example, hydrogel-based biomaterials with ce...

Encapsulation of enzymes allows to preserve their biological activities in various environmental conditions, such as exposure to elevated temperature or to proteases. This is particularly relevant for in vivo applications, where proteases represent a severe obstacle to maintaining the activity of enzymes. Polyelectrolyte multilayer capsules are sui...

Whey protein isolate (WPI) is a by-product from the production of cheese and Greek yoghurt comprising β-lactoglobulin (β-lg) (75%). Hydrogels can be produced from WPI solutions through heating; hydrogels can be sterilized by autoclaving. WPI hydrogels have shown cytocompatibility and ability to enhance proliferation and osteogenic differentiation o...

Implementation of an effective focal cell adhesion represents a significant challenge because it requires to develop appropriate materials and processes together with assuring that cells would interact with it effectively. Various coatings are under development in the area of biomaterials including hydrogels and polymeric surfaces. Here, we analyse...

Biomaterials engineered with specific cell binding sites, tunable mechanical properties, and complex architectures are essential to control cell adhesion and proliferation. The influence of the local properties, such as the local hardness and stability on the interaction with cells, has not been yet fully understood and exploited. This is particula...

Сomposite bioceramic and hydrogel-based containers harbouring alkaline phosphatase are generated through encapsulation of this enzyme by its immobilization into CaCO3-based bioceramic materials in combination with a hydrogel assembly technique and subsequent gelification. A refined way of synthesis and modification allows preparing the enzyme deliv...

Mineralization of hydrogel biomaterials with calcium phosphate (CaP) is considered advantageous for bone regeneration. Mineralization can be both induced by the enzyme alkaline phosphatase (ALP) and promoted by calcium-binding biomolecules, such as plant-derived polyphenols. In this study, ALP-loaded gellan gum (GG) hydrogels were enriched with gal...

In this review, materials based on polymers and hybrids possessing both organic and inorganic contents for repairing or facilitating cell growth in tissue engineering are discussed. Pure polymer based biomaterials are predominantly used to target soft tissues. Stipulated by possibilities of tuning the composition and concentration of their inorgani...

The research presents the designing new hybrid biocompatible materials aimed to bone tissue engineering with enhanced osteoconductivity and functionality. The scaffolds consisted of electrospun polymeric matrix, modified with porous calcium carbonate (vaterite) coatings, were developed and studied. The subcutaneous implantation tests in vivo with w...

Polyelectrolyte multilayer (PEM) capsules, constructed by LbL (layer-by-layer)-adsorbing polymers on sacrificial templates, have become important carriers due to multifunctionality of materials adsorbed on their surface or encapsulated into their interior. They have been also been used broadly used as analytical tools. Chronologically and tradition...

Surface-enhanced Raman scattering (SERS) is a high-performance technique allowing detection of extremely low concentrations of analytes. For such applications, fibrous polymeric matrices decorated with plasmonic metal nanostructures can be used as flexible SERS substrates for analysis of analytes in many application. In this study, a three-dimensio...

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Multifunctional probes play an increasing role even beyond applications in biomedicine. Multifunctionality introduced by the dual types of complementary probes is always attractive because, in this case, functionalized objects inherit the function of both materials. Porous calcium carbonate microparticles are becoming popular carriers of biomolecul...

Modification of titanium (Ti) bone implant materials with hybrid organic-inorganic coatings is the novel promising approach to improve osteoconductivity and osteointegration of implants and prevent their failure after implantation. However, in these coatings, which are mostly hydrophilic, chemically active moieties capable to release oxidizing ions...

We studied the particularities of osteo- and angiogenesis in albino rats after implantation of polycaprolactone scaffolds mineralized with vaterite with adsorbed tannic acid in the femoral bone defect. It was found that the processes of angio- and osteogenesis in the bone tissue after scaffolds implantation depend on their biocompatibility. Implant...

10.1021/acsami.9b04936Elaboration of novel biocomposites providing simultaneously both biodegradability and stimulated bone tissue repair is essential for regenerative medicine. In particular, piezoelectric biocomposites are attractive because of a possibility to electrically stimulate cell response. In the present study, novel CaCO3-mineralized pi...

Transdermal administration via skin appendages enables both localized and systemic drug delivery, as well as minimizes incidental toxicity. However, the design of an appropriate effective method for clinical use remains challenging. Here, we introduce calcium carbonate-based carriers for the transdermal transportation of bioactive substances. The p...

The importance of thermodynamics does not need to be emphasized. Indeed, elevated temperature processes govern not only industrial scale production, but also self-assembly, chemical reaction, interaction between molecules, etc. Not surprisingly, biological processes take typically place at defined temperature. Here, we look at possibilities to rais...

Hybrid materials, or hybrids incorporating both organic and inorganic constituents, are emerging as a very potent and promising class of materials due to the diverse, but complementary nature of the properties inherent of these different classes of materials. The complementarity leads to a perfect synergy of properties of desired material and event...

Abstract We demonstrate a novel approach to the controlled loading of inorganic nanoparticles and proteins into submicron- and micron-sized porous particles. The approach is based on freezing/thawing cycles, which lead to high loading densities. The process was tested for the inclusion of Au, magnetite nanoparticles, and bovine serum albumin in bio...

This article reports on a study of the mineralisation behaviour of CaCO 3 deposited on electrospun poly(ε-caprolactone) (PCL) scaffolds preliminarily treated with low-temperature plasma.

We have designed sensors based on Ag nanoparticles synthesized in situ on the vaterite beads. In this article we demonstrate an approach to produce size controllable spherical and elliptical vaterite particles and discuss time-dependent in situ Ag nanoparticles synthesis and its potential effect on surface-enhanced Raman scattering. The time depend...

The enhancement of the Raman light scattering signal from the surface of the porous hybrid biocomposites based on polyhydroxybutyrate (PHB), calcium carbonate (CaCO3), and nanoplasmonic Ag particles is investigated. Based on PHB, fibrous scaffolds are obtained by the electrospinning technique. The fibrous scaffolds have been covered by CaCO3 and Ag...

Non-destructive, controllable, remote light-induced release inside cells enables studying time- and space- specific processes in biology. In this work we demonstrate remote release of tagged proteins in Caenorhabditis elegans (C. elegans) worms using a near-infrared laser light as a trigger from novel hydrogel shells functionalized with silver nano...

The aim is to assess skin microcirculation changes arising during subcutaneous implantation of polycaprolactone scaffolds mineralized by vaterite. The experiment was carried out on 30 albino rats divided into two groups: a negative control group and an experimental one. We implanted polycaprolactone scaffold with exhausted foreign protein subcutane...

Designing advanced biomaterials for tissue regeneration with drug delivery and release functionalities remains a challenge in regenerative medicine. In this research, we have developed novel composite scaffolds based on polymeric polycaprolactone fibers coated with porous calcium carbonate structures (PCL/CaCO3) for tissue engineering and have show...

Calcium carbonate (CaCO3) attracts scientific attention due to its essential role in both inorganic and bioorganic nature. Vaterite is the least thermodynamically stable CaCO3 polymorph, elicited great interest as an advanced biomaterial for tissue engineering, drug delivery and a broad range of personal care products. Numerous methods of vaterite...

Mineralized hydrogels are increasingly gaining attention as biomaterials for bone regeneration. The most common mineralization strategy has been addition of preformed inorganic particles during hydrogel formation. This maintains injectability. One common form of bone cement is formed by mixing particles of the highly reactive calcium phosphate alph...

Abstract FIELD: medicine. SUBSTANCE: for the transdermal delivery of biologically active substances (BAS), the application of containers with immobilized BAS to the surface of the skin is carried out, followed by transportation through the appendages of the skin. Porous calcium carbonate particles in a crystallographic modification of a vaterite wi...