Bo-Jui Chang

Bo-Jui Chang
University of Texas Southwestern Medical Center | UT Southwestern · Lyda Hill Department of Bioinformatics

Ph.D

About

52
Publications
14,275
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
703
Citations
Citations since 2017
34 Research Items
608 Citations
2017201820192020202120222023050100150
2017201820192020202120222023050100150
2017201820192020202120222023050100150
2017201820192020202120222023050100150
Additional affiliations
September 2017 - present
University of Texas Southwestern Medical Center
Position
  • Instructor
Description
  • Light sheet fluorescence microscopy (LSFM), Super-resolution structured illumination microscopy (SR-SIM),
July 2016 - August 2017
Academia Sinica
Position
  • PostDoc Position
Description
  • lattice light sheet microscopy
September 2011 - June 2016
Goethe-Universität Frankfurt am Main
Position
  • PostDoc Position
Description
  • super-resolution structured illumination microscopy (SR-SIM), light sheet-based fluorescence microscopy (LSFM), coherent structured illumination light sheet-based fluorescence microscopy (csiLSFM)

Publications

Publications (52)
Article
Full-text available
Significance Structured illumination microscopy (SIM) is a superresolution technique that illuminates the specimen with a sine-modulated pattern. The frequency of the illumination pattern, i.e., the inverse of the resolution, is limited by the angular aperture of the objective lens. SIM in combination with light-sheet-based fluorescence microscopy...
Article
In light sheet-based fluorescence microscopy (LSFM), only the focal plane is illuminated by a laser light sheet. Hence, only the fluorophores within a thin volume of the specimen are excited. This reduces photo-bleaching and photo-toxic effects by several orders of magnitude compared with any other form of microscopy. Therefore, LSFM (aka single/se...
Article
Full-text available
We developed a structured illumination microscopy (SIM) system that uses a spatial light modulator (SLM) to generate interference illumination patterns at four orientations - 0 degrees, 45 degrees, 90 degrees, and 135 degrees, to reconstruct a high-resolution image. The use of a SLM for pattern alterations is rapid and precise, without mechanical c...
Article
Full-text available
Structured illumination microscopy relies on reconstruction algorithms to yield super-resolution images. Artifacts can arise in the reconstruction and affect the image quality. Current reconstruction methods involve a parametrized apodization function and a Wiener filter. Empirically tuning the parameters in these functions can minimize artifact...
Article
Type 3 fimbriae are important adhesive filaments that assist Klebsiella pneumoniae to establish an infection. Different MrkD adhesin variants on the fimbriae are known to display distinct adherence capability for the bacteria to bind extracellular matrix proteins, although the difference has not been determined physically. For this reason, the adhe...
Article
Full-text available
Structured illumination microscopy (SIM) doubles the spatial resolution of a fluorescence microscope without requiring high laser powers or specialized fluorophores. However, the excitation of out-of-focus fluorescence can accelerate photobleaching and phototoxicity. In contrast, light-sheet fluorescence microscopy (LSFM) largely avoids exciting ou...
Article
Tissue microenvironments affect the functional states of cancer cells, but determining these influences in vivo has remained a challenge. We present a quantitative high-resolution imaging assay of single cancer cells in zebrafish xenografts to probe functional adaptation to variable cell-extrinsic cues and molecular interventions. Using cell morpho...
Article
The ligand-activated transcription factor aryl hydrocarbon receptor (AHR) regulates cellular detoxification, proliferation and immune evasion in a range of cell types and tissues, including cancer cells. In this study, we used RNA-sequencing to identify the signature of the AHR target genes regulated by the pollutant 2,3,7,8-tetrachlorodibenzodioxi...
Article
Fast volumetric imaging of large fluorescent samples with high-resolution is required for many biological applications. Oblique plane microscopy (OPM) provides high spatiotemporal resolution, but the field of view is typically limited by its optical train and the pixel number of the camera. Mechanically scanning the sample or decreasing the overall...
Preprint
Full-text available
Structured illumination microscopy (SIM) doubles the spatial resolution of a fluorescence microscope without requiring high laser powers or specialized fluorophores. However, the excitation of out-of-focus fluorescence can accelerate photobleaching and phototoxicity. In contrast, light-sheet fluorescence microscopy (LSFM) largely avoids exciting ou...
Article
Light sheet fluorescence microscopy (LSFM) uses a thin sheet of light to excite only fluorophores within the focal volume. Light sheet microscopes (LSMs) have a true optical sectioning capability and, hence, provide axial resolution, restrict photobleaching and phototoxicity to a fraction of the sample and use cameras to record tens to thousands of...
Article
Full-text available
We introduce a cost-effective and easily implementable scan unit that converts any camera-based microscope with optical sectioning capability into a multi-angle projection imaging system. Projection imaging reduces data overhead and accelerates imaging by a factor of >100, while also allowing users to readily view biological phenomena of interest f...
Preprint
A key challenge in cancer research is to identify functional cell states as they relate to specific tissue microenvironments. In this work, we present a quantitative high-resolution imaging assay of cancer cell morphology in zebrafish xenografts to probe functional adaptation to variable cell extrinsic cues and molecular interventions. We focus on...
Preprint
Full-text available
For most human cells, anchorage is a key necessity for survival. Cell-substrate adhesion activates diverse signaling pathways, without which cells undergo anoikis – a form of programmed cell death ¹ . Acquisition of anoikis resistance is a pivotal step in cancer disease progression, as metastasizing cancer cells often lose firm attachment to surrou...
Article
Full-text available
We present an Oblique Plane Microscope that uses a bespoke glass-tipped tertiary objective to improve the resolution, field of view, and usability over previous variants. Owing to its high numerical aperture optics, this microscope achieves lateral and axial resolutions that are comparable to the square illumination mode of Lattice Light-Sheet Micr...
Preprint
Full-text available
We introduce a cost-effective and easily implemented scan unit which enables any camera-based microscope to perform projection imaging from diverse viewing angles. We demonstrate this capability on Lattice Light-Sheet and Oblique Plane Microscopy by rapidly delivering projection images with an uncompromised lateral resolution and high optical contr...
Article
Full-text available
In optical microscopy, the slow axial scanning rate of the objective or the sample has traditionally limited the speed of volumetric imaging. Recently, by conjugating either a movable mirror to the image plane in a remote-focusing geometry or an electrically tuneable lens (ETL) to the back focal plane, rapid axial scanning has been achieved. Howeve...
Article
The axial resolving power of a light-sheet microscope is determined by the thickness of the illumination beam and the numerical aperture of its detection optics. Bessel-beam based optical lattices have generated significant interest owing to their reportedly narrow beam waist and propagation-invariant characteristics. Yet, despite their significant...
Preprint
Full-text available
The axial resolving power of a light-sheet microscope is determined by the thickness of the illumination beam and the numerical aperture of its detection optics. Bessel-based optical lattices have generated significant interest owing to their potentially narrow beam waist and propagation-invariant characteristics. Yet, despite their significant use...
Conference Paper
Ewing Sarcoma (ES), a pediatric cancer driven by the oncogenic fusion protein EWS-FLI1, recurs or metastasizes in 1 in 3 patients with no clear correlation to accumulation of secondary mutations, raising the possibility that environmental cues may drive metastasis. Previous work in the field has observed distinct cellular morphologies of ES cells i...
Preprint
Full-text available
We present a single-objective light-sheet microscope, also known as an oblique-plane microscope, that uses a bespoke glass-tipped tertiary objective and improves the resolution, field of view, usability, and stability over previous variants. Owing to its high numerical aperture optics, this microscope achieves the highest lateral resolution in ligh...
Preprint
Full-text available
In optical microscopy, the slow axial scanning rate of the objective or the sample has traditionally limited the speed of 3D volumetric imaging. Recently, by conjugating either a movable-mirror to the image plane or an electrotuneable lens (ETL) to the back-focal plane respectively, rapid axial scanning has been achieved. However, mechanical actuat...
Article
Full-text available
Recent advances in light-sheet microscopy have enabled sensitive imaging with high spatiotemporal resolution. However, the creation of thin light-sheets for high axial resolution is challenging, as the thickness of the sheet, field of view and confinement of the excitation need to be carefully balanced. Some of the thinnest light-sheets created so...
Article
Full-text available
We present cleared-tissue axially swept light-sheet microscopy (ctASLM), which enables isotropic, subcellular resolution imaging with high optical sectioning capability and a large field of view over a broad range of immersion media. ctASLM can image live, expanded, and both aqueous and non-aqueous chemically cleared tissue preparations. Depending...
Article
Full-text available
Combining super-resolution structured illumination microscopy (SIM) and lattice lightsheet microscopes (LLSMs) has always been an ideal approach for high spatiotemporal resolution in 3D applications. We propose a simpler method to perform 2D-SIM with three phases, which is 5/3 faster and less sensitive to optical alignment compared to 3D-SIM in LLS...
Preprint
Full-text available
Cells modify their shape in response to the extracellular environment through dynamic remodeling of the actin cytoskeleton by actin-binding proteins (ABPs) 1-4. The relation between actin dynamics and spreading is well-understood for cells on flat glass coverslips; much less is known about cell morphogenesis in compliant three-dimensional environme...
Preprint
Full-text available
Recent advances in light-sheet microscopy have enabled sensitive imaging with high spatiotemporal resolution. However, the creation of thin light-sheets for high axial resolution is challenging, as the thickness of the sheet, field of view and confinement of the excitation need to be carefully balanced. Some of the thinnest light-sheets created so...
Article
Full-text available
Background Every biological experiment requires a choice of throughput balanced against physiological relevance. Most primary drug screens neglect critical parameters such as microenvironmental conditions, cell-cell heterogeneity, and specific readouts of cell fate for the sake of throughput. Methods Here we describe a methodology to quantify prol...
Preprint
Full-text available
We present cleared tissue Axially Swept Light-Sheet Microscopy (ctASLM), which achieves sub-micron isotropic resolution, high optical sectioning capability, and large field of view imaging (870×870 µm2 ) over a broad range of immersion media. ctASLM can image live, expanded, and both aqueous and organic chemically cleared tissue preparations and pr...
Article
Full-text available
We introduce field synthesis, a theorem and method that can be used to synthesize any scanned or dithered light sheet, including those used in lattice light-sheet microscopy (LLSM), from an incoherent superposition of one-dimensional intensity distributions. Compared to LLSM, this user-friendly and modular approach offers a simplified optical desig...
Preprint
Full-text available
We introduce Field Synthesis, a theorem that can be used to synthesize any scanned or dithered light-sheet, including those used in lattice light-sheet microscopy (LLSM), from an incoherent superposition of one-dimensional intensity distributions. This user-friendly and modular approach offers a drastically simplified optical design, higher light-t...
Preprint
Full-text available
Background Every biological experiment requires a choice of throughput balanced against physiological relevance. Most primary drugs screens neglect critical parameters such as microenvironmental conditions, cell-cell heterogeneity, and specific readouts of cell fate for the sake of throughput. Methods Here we describe a methodology to quantify pro...
Article
Full-text available
Structured illumination microscopy (SIM) was recently adapted to coherent imaging, named structured oblique-illumination microscopy (SOIM), to improve the contrast and resolution of a light-scattering image. Herein, we present high-resolution laterally isotropic SOIM imaging with 2D hexagonal illuminations. The SOIM is implemented in a SIM fluoresc...
Article
Full-text available
Three-dimensional structured illumination microscopy (3D-SIM) is a wide-field technique that can provide doubled resolution and improved image contrast. In this work, we demonstrate a simple approach to 3D-SIM - using three-beam interference with circular polarization to generate the pattern of structured illumination, so that the modulation contra...
Patent
Full-text available
The present invention discloses an optical system to generate incoherent structured illumination and an optical imaging system using incoherent structured illumination. The optical system includes: at least one coherent light source, a spatial light modulator, a plurality of optical lenses, a rotating diffuser for destroying the coherence of the st...
Article
Bioconjugated fluorescent nanodiamonds (FNDs) show potential for effectively targeted imaging and the enhanced photokilling of cancer cells. In this study, we investigated the mechanisms involved in the cellular uptake of surface-modified 140 nm FNDs and evaluated their cytotoxicity and phototoxicity following particle internalization. Through an a...
Article
Full-text available
A reflective light-scattering (RLS) microscope with structured illumination (SI) provides subdiffraction resolution and improves the image quality of gold nanoparticles in biological systems. The three-dimensional (3D)-structured pattern is rapidly and precisely controlled with a spatial light modulator and scrambled at the conjugate image plane to...
Thesis
雷射鑷夾由於其非侵入性及非機械破壞性的特點,且及所產生的捕捉力大小 接近許多生物力的範圍,因此常被應用於細胞與分子生物的研究中。在本論文 中,我們利用雷射鑷夾研究克雷白氏肺炎桿菌單一根第三型線毛與第四型膠原蛋 白的黏附力。線毛利用頂端的黏著素來黏附,而第三型線毛帶有四種不同的黏著 素。藉由雷射鑷夾,我們首次量測出這四種黏著素對單一根線毛與膠原蛋白的黏 附力:其中帶有MrkDv1 黏著素的線毛幾乎與膠原蛋白沒有黏附力,而帶有 MrkDv2、MrkDv3、及MrkDv4 黏著素的線毛與膠原蛋白的黏附力分別為2.03±0.03 pN,3.79±0.12 pN,及 2.87 ± 0.15 pN。我們另外也應用了雷射鑷夾於整合素αⅡbβ3 與蛇毒蛋白的黏附力量測。結果發現單一個野生型的蛇毒蛋白與整合素...
Article
Full-text available
Recent evidence demonstrated that conformational changes of the integrin during receptor activation affected its binding to extracellular matrix; however, experimental assessment of ligand-receptor binding following the initial molecular interaction has rarely been carried out at a single-molecule resolution. In the present study, laser tweezers we...
Article
The automation of a rapid and simple trapping-force calibration system is desired for optical tweezers to measure biological forces. One simple calibration method, the water-dragging-force method, is to calibrate the trapping force against a given water dragging force with an image-processing technique. However, the conventional image-processing te...
Conference Paper
Swimming activity of flagella is a main factor of the motility of bacteria. Flagella expressed on the surface of bacterial species serve as a primary means of motility including swimming. We propose to use optical tweezers to analyze the swimming activity of bacteria. The sample bacteria in the work is Pseudomonas aeruginosa, and it is a gram-negat...
Conference Paper
The conformational change of integrin αIIbβ3 plays an important role in clot formation. However, the correlation between the structure and the function of integrin αIIbβ3 in interacting with its ligand is still not clear. In this report, we focus on the dynamic variation of the binding between integrin αIIbβ3 and its ligand, rhodostomin by using a...
Conference Paper
Integrin receptors serve as both mechanical links and signal transduction mediators between the cell and its environment. Experimental evidence demonstrates that conformational changes and lateral clustering of the integrin proteins may affect their binding to ligands and regulate downstream cellular responses; however, experimental links between t...
Conference Paper
Sample tracking with a high spatial sensitivity is highly desired in force measurement with optical tweezers. However, the trick that sample tracking via forward scattering pattern detection would provide a higher sensitivity than that via regular image detection has never been investigated. In this paper, we systematically study the influences of...
Thesis
A typical optical tweezers 1 system is operated by precisely focusing a laser beam through an objective into a laser point on the sample plane of a microscope. This results in the trap of a single particle into the laser point via gradient dipole force 2 . In the past decade, such in-focus optical tweezers have been widely used in capturing, moving...
Conference Paper
Full-text available
Optical tweezers is a newly developed instrument, which makes possible the manipulation of micro-optical particles under a microscope. In this paper, we present the automation of an optical tweezers which consists of a modified optical tweezers, equipped with two motorized actuators to deflect a 1 W argon laser beam, and a computer control system i...

Questions

Question (1)
Question
I would like to find a laser to excite fluorophores in fluorescence microscope.  However, I also need to perform interference with this laser.  Due to the difficulty of aligning the system, the coherence length is better to have about a few centimeters.  Usually, the longer the coherence length also means the narrower the linewidth, which will then be less efficient for fluorophores excitation.  Therefore, I am asking if there is a compromised solution, or is there some ways to extend the coherence length of a semiconductor laser. I would need a laser at around 470 - 500 nm to excite green fluorescence protein (GFP).

Network

Cited By