Bo Dong

Bo Dong
Ocean University of China | OUC · Department of Marine Biology

PhD

About

123
Publications
19,546
Reads
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2,449
Citations
Citations since 2017
52 Research Items
1313 Citations
2017201820192020202120222023050100150200250
2017201820192020202120222023050100150200250
2017201820192020202120222023050100150200250
2017201820192020202120222023050100150200250
Additional affiliations
November 2010 - September 2014
RIKEN
Position
  • Dr.
October 2006 - October 2010
University of Bergen
Position
  • PostDoc Position
June 2000 - October 2006
Chinese Academy of Sciences
Position
  • Dr.

Publications

Publications (123)
Article
Full-text available
Significance Robust pattern formation is a ubiquitous question of developmental biology. In 1952, Turing proposed in a seminal paper that this could be achieved by a hydrodynamical instability of two diffusing species reacting with each other, but direct experimental evidence of how a mechanism is implemented biologically is still lacking. In this...
Article
Full-text available
Tunicates occupy the evolutionary position at the boundary of invertebrates and vertebrates. It exhibits adaptation to broad environmental conditions and is distributed globally. Despite hundreds of years of embryogenesis studies, the genetic basis of the invasive habits of ascidians remains largely unknown. The leathery sea squirt, Styela clava, i...
Article
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Ventral bending of the embryonic tail within the chorion is an evolutionarily conserved morphogenetic event in both invertebrates and vertebrates. However, the complexity of the anatomical structure of vertebrate embryos makes it difficult to experimentally identify the mechanisms underlying embryonic folding. This study investigated the mechanisms...
Article
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Evolutionary developmental biology, or Evo-Devo for short, has become an established field that, broadly speaking, seeks to understand how changes in development drive major transitions and innovation in organismal evolution. It does so via integrating the principles and methods of many subdisciplines of biology. Although we have gained unprecedent...
Article
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Evolutionary developmental biology (evo-devo) has been among the most fascinating interdisciplinary fields for decades, which aims to elucidate the origin and evolution of diverse developmental processes. The rapid accumulation of omics data provides unprecedented opportunities to answer many interesting but unresolved evo-devo questions. However,...
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Halocynthia roretzi, the edible ascidian, has been demonstrated to be an important source of bioactive natural metabolites. Here, we reported a novel terpenoid compound named Halorotetin A that was isolated from tunic ethanol extract of H. roretzi by silica gel column chromatography, preparative layer chromatography (PLC), and semipreparative-HPLC....
Article
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The glycoprotein hormone (GPH) system is fundamentally significant in regulating the physiology of chordates, such as thyroid activity and gonadal function. However, the knowledge of the GPH system in the primitive chordate ascidian species is largely lacking. Here, we reported an ancestral GPH system in the ascidian (Styela clava), which consists...
Article
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Osmoregulation is essential for organisms to adapt to the exterior environment and plays an important role in embryonic organogenesis. Tubular organ formation usually involves a hyperosmotic lumen environment. The mechanisms of how the cells respond and regulate lumen formation remain largely unknown. Here, we reported that the nuclear factor of ac...
Article
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Actin is a ubiquitous protein and plays essential roles on cellular structure maintenance and cellular motility in both muscle and non-muscle tissues. Multiple genes encoding muscle actin have been identified from the ascidians, including those expressed in the larval tail muscle, the adult body-wall muscle, and adult heart muscle. In this study, a...
Article
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Halocynthia roretzi has been cultured and utilized as a nutritious seafood in Southeastern Asia for many years because of its abundance active molecules. Here, we reported that feeding of H. roretzi tissues resulted in improving oral glucose tolerance in rats. To screen the active components, we identified 950 chemical compounds from H. roretzi met...
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Objectives: CDC45 is the core component of CMG (CDC45-MCMs-GINS) complex that plays important role in the initial step of DNA replication in eukaryotic cells. The expression level of cdc45 is under the critical control for the accurate cell cycle progression. Loss-of-function of cdc45 has been demonstrated to inhibit cell proliferation and leads t...
Preprint
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Zygotic genome activation (ZGA), a universal process in early embryogenesis that occurs during the maternal-to-zygotic transition, involves reprogramming in the zygotic nucleus that initiates global transcription. In recent decades, knowledge of this process has been acquired from research on various model organisms; however, a consensus explanatio...
Article
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In this study, we performed the genome-wide domain analysis and sequence alignment on the genome of Styela clava, and obtained a repertoire of 204 putative GPCRs, which exhibited a highly reduced gene number compared to vertebrates and cephalochordates. In this repertoire, six Class C GPCRs, including four metabotropic glutamate receptors (Sc-GRMs)...
Article
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It has long been known that abundant symbiotic bacteria exist in the tunic and gut of marine ascidians, and that these play crucial roles in host development, physiological metabolism, and environmental adaptation. However, the identity, roles and functions of these symbiotic bacteria are known for only a few strains. In this study, we isolated and...
Article
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Marine ascidian is becoming one of the main sources of an antitumor drug that has shown high bioactivity and extensive application in cancer treatment. Halocynthia roretzi, an edible marine sea squirt, has been demonstrated to have various kinds of biological activities, such as anti-diabetic, anti-hypertension, and enhancing immunity. In this stud...
Article
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Synthesis of cellulose and formation of tunic structure are unique traits in the tunicate animal group. However, the regulatory mechanism of tunic formation remains obscure. Here, we identified a novel microRNA cluster of three microRNAs, including miR4018a, miR4000f, and miR4018b in Ciona savignyi. In situ hybridization and promoter assays showed...
Article
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The phylum of Chordata is defined based on the discovery of a coelom-like dorsal notochord in ascidian and amphioxus embryos. Chordata can be classified into three subphylums, Cephalochordata, Urochordata, and Vertebrata, united by the presence of a notochord at some point during development. The origin of the notochord, the signature anatomical st...
Article
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MicroRNAs (miRNAs) regulate multicellular processes and diverse signaling pathways in organisms. The detection of the spatiotemporal expression of miRNA in vivo is crucial for uncovering the function of miRNA. However, most of the current detecting techniques cannot reflect the dynamics of miRNA sensitively in vivo. Here, we constructed a miRNA-ind...
Article
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The sea potato Acaudina spp. is a species of sea cucumber, belonging to the class Holothuroidea under Phylum Echinodermata. It becomes blooming recently in the East Sea of China, causing serious ecological problems. However, until now there are no molecular data for its larval identification and population genetic analysis. In this study, we firstl...
Article
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Tunicates include diverse species, as they are model animals for evolutionary developmental biology study. The embryonic development of tunicates is known to be extensively regulated by transcription factors (TFs). Styela clava, the globally distributed invasive tunicate, exhibits a strong capacity for environmental adaptation. However, the TFs wer...
Article
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MicroRNAs are frequently clustered in the genome and polycistronically transcribed, regulating targeted genes in diverse signaling pathways. The miR-17-92 cluster is a typical miRNA cluster, playing crucial roles in the organogenesis and homeostasis of physiological processes in vertebrates. Here, we identified three miRNAs (csa-miR-92a, csa-miR-92...
Article
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Progressive unfolding of gene expression cascades underlies diverse embryonic lineage development. Here, we report a single-cell RNA sequencing analysis of the complete and invariant embryonic cell lineage of the tunicate Ciona savignyi from fertilization to the onset of gastrulation. We reconstructed a developmental landscape of 47 cell types over...
Article
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Cell and tissue polarity due to the extracellular signaling and intracellular gene cascades, in turn, signals the directed cell behaviors and asymmetric tissue architectures that play a crucial role in organogenesis and embryogenesis. The notochord is a characteristic midline organ in chordate embryos that supports the body structure and produces p...
Article
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Background Gut microbiota plays important roles in host animal metabolism, homeostasis and environmental adaptation. However, the interplay between the gut microbiome and urochordate ascidian, the most closet relative of vertebrate, remains less explored. In this study, we characterized the gut microbial communities of urochordate ascidian (Halocyn...
Article
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The transient larva-bearing biphasic life cycle is the hallmark of many metazoan phyla, but how metazoan larvae originated remains a major enigma in animal evolution. There are two hypotheses for larval origin. The ‘larva-first’ hypothesis suggests that the first metazoans were similar to extant larvae, with later evolution of the adult-added bipha...
Article
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An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Preprint
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Background Gut microbiota plays important roles in host animal physiology, homeostasis, metabolism, and environmental adaptation while the interplay between the gut microbiome and urochordate ascidian, the most closet relative of vertebrate, remains less explored. In this study, we characterized the gut microbial communities of urochordate ascidian...
Preprint
Full-text available
Background Gut microbiota plays important roles in host animal metabolism, homeostasis and environmental adaptation. However, the interplay between the gut microbiome and urochordate ascidian, the most closet relative of vertebrate, remains less explored. In this study, we characterized the gut microbial communities of urochordate ascidian ( Halocy...
Article
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Abstract Ascidians are sessile marine chordate invertebrates found along seashores worldwide and are typically regarded as invasive organisms. Knowledge concerning their global genetic structure and subsequent invasive potential is limited. Here, we identified three ascidians—Ciona robusta, Ciona savignyi, and Styela clava from the northeast region...
Preprint
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In multicellular organisms, a single zygote develops along divergent lineages to produce distinct cell types. What governs these processes is central to the understanding of cell fate specification and stem cell engineering. Here we used the protochordate model Ciona savignyi to determine gene expression profiles of every cell of single embryos fro...
Article
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Objective The relationship between genomic variables (genome size, gene number, intron size, and intron number) and evolutionary forces has two implications. First, they help to unravel the mechanism underlying genome evolution. Second, they provide a solution to the debate over discrepancy between genome size variation and organismal complexity. P...
Article
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Cell morphology and tissue integrity are essential for embryogenesis. Caveolins are membrane proteins that induce the formation of surface pits called caveolae that serve as membrane reservoirs for cell and tissue protection during development. In vertebrates, caveolin 1 (Cav1) and caveolin 3 (Cav3) are required for caveola formation. However, the...
Article
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Marine ascidians are becoming important drug sources that provide abundant secondary metabolites with novel structures and high bioactivities. As one of the most chemically prolific marine animals, more than 1200 inspirational natural products, such as alkaloids, peptides, and polyketides, with intricate and novel chemical structures have been iden...
Article
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Morphogenesis is a process describing how the shapes of living tissues and bodies are created during development. Living and fossil organisms exhibit enormously diverse tissue architecture and body forms, although the functions of organs are evolutionally conserved. Current knowledge reveals that relatively conserved mechanisms are applied to contr...
Article
Lactadherin is an extracellular matrix glycoprotein with stimulating agglutination ability that plays crucial roles in animal immunology. In the present study, a novel lactadherin, Sc-lactadherin, was identified from the marine invertebrate chordate, Styela clava. Its full-length cDNA consisted of 579 bps, encoding 193 amino acids with a coagulatio...
Article
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The solute carrier 6 (SLC6) gene family, functioning as neurotransmitter transporters, plays the crucial roles in neurotransmission, cellular and organismal homeostasis. In this study, we found an expansion of SLC6 family gene in the genome of chordate invertebrate Ciona savignyi. A total of 40 candidate genes including 29 complete and 11 putative...
Article
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The sea potato (Acaudina molpadioides) is a species of sea cucumber of the family Holothuroidea under Phylum Echinodermata. In this study, we firstly sequenced and annotated the complete mitochondrial genome of A. molpadioides, which was 16,028 bps in size and contained 13 protein-coding genes, 2 rRNAs, 19 tRNA genes, and 1 putative control region....
Article
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Adhesion is essential for many marine sessile organisms. Unraveling the compositions and assembly of marine bioadheisves is the fundamental to understand their physiological roles. Despite the remarkable diversity of animal bioadhesion, our understanding of this biological process remains limited to only a few animal lineages, leaving the majority...
Article
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Adhesion is a vital physiological process for many marine molluscs, including the mussel and scallop, and therefore it is important to characterize the proteins involved in these adhesives. Although several mussel byssal proteins were identified and characterized, the study for scallop byssal proteins remains scarce. Our previous study identified t...
Article
Long noncoding RNAs (lncRNAs) play important roles in diverse developmental and pathological processes through chromatin reprogramming, cis regulation and posttranscriptional modification. They have been extensively studied in both vertebrates and invertebrates. However, the information of lncRNAs in urochordate is still lacking. In this study, we...
Article
Examining the physiological responses of mussels to thermal stress is crucial to evaluate their biogeographic distribution and ability to adapt to a changing climate. In the present study, we investigated the effects of acute cold (8 °C and 15 °C) and heat (35 °C and 42 °C) stress on the mortality rate, reactive oxygen species (ROS) production, mal...
Article
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Background: Metamorphosis takes place within the life cycle of most marine invertebrates. The marine ascidian is a classical model to study complex cellular processes and underlying molecular mechanisms involved in its larval metamorphosis. The detailed molecular signaling pathways remain elusive, though extracellular signal-regulated kinases (ERK...
Article
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Chondromodulin-1 (ChM-1) is an extracellular matrix protein that plays crucial roles in tumor cell growth and angiogenesis in vertebrates and humans. ChM-1 is highly expressed in the invertebrate Ciona savignyi, a marine ascidian chosen as a model. The effect of the recombinant Ciona mature ChM-1 peptide (Cs-mChM-1) on cell proliferation, migration...
Article
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The extracellular matrix (ECM) not only provides essential physical scaffolding for cellular constituents but also initiates crucial biochemical and biomechanical cues that are required for tissue morphogenesis. In this study, we utilized wheat germ agglutinin (WGA) staining to characterize the ECM architecture in ascidian embryos and larvae. The r...
Article
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Reconstructing the genomes of bilaterian ancestors is central to our understanding of animal evolution, where knowledge from ancient and/or slow-evolving bilaterian lineages is critical. Here we report a high-quality, chromosome-anchored reference genome for the scallop Patinopecten yessoensis, a bivalve mollusc that has a slow-evolving genome with...
Article
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For efficient respiration, tubular airways must be constructed with an optimal diameter and length for the dimensions of the body. In Drosophila, the growth of embryonic tracheal tubules proceeds in two dimensions, by axial elongation and diameter expansion. The growth forces in each dimension are controlled by distinct genetic programs and cellula...
Article
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Spliceosomal introns are present in diverse eukaryotic but not prokaryotic genes. However, their evolutionary origin is still arguable. Here, we address this question through an analysis of the cellulose synthase gene, which is present only in urochordate among the animal lineages due to horizontal transfer from a bacterial ancestor in the distant...
Article
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Coral has strong regeneration ability, which has been applied for coral production and biodiversity protection via tissue ball (TB) culture. However, the architecture, morphological processes, and effects of environmental factors on TB formation have not been well investigated. In this study, we first observed TB formation from the cutting tentacle...
Article
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Connection of tubules into larger networks is the key process for the development of circulatory systems. In Drosophila development, tip cells of the tracheal system lead the migration of each branch and connect tubules by adhering to each other and simultaneously changing into a torus-shape. We show that as adhesion sites form between fusion cells...
Data
Time course of dorsal branch migration and fusion and their relationship to the epidermis. GFP-moe was expressed in tracheal FCs and pAs by esg_FC-Gal4, and in the sqh-GFP-moe. Lower panel corresponds to a stacked x-z section in the boxed area of dorsal view in top panel. Scale bar in lower right is 10 μm and time stamp is shown in top-right. Origi...
Data
Effect of Kat60 on microtubule stability visualized by tau-RFP imaging. Left: control, right: Kat60 expressed by btl-Gal4. Related to Fig. 4F.
Data
Double labeling of tau-RFP (top, red) and GFP-moe (middle, green) driven by btl-Gal4. Related to Fig. 1e.
Data
Effect of myosin inhibition on fusion of DB. GFP-DN-Zip was co-expressed with p120ctn-RFP by btlGal4. Top panel; x-y view, bottom panel: y-z view. Most of FCs reached the midline and accumulated p120ctn-RFP at the interface. But p120ctn-RFP was not concentrated into a spot and FC failed to contract. Original movie used to prepare Fig. 3d.
Data
Dynamics of microtubule plus end. FC and TC of btl > EB1-GFP embryo were imaged. The branch was directed toward 10 o'clock direction. Left: original movie. Right: overlay of PIV tracks. Original movie used to prepare Fig. 4c.
Data
Effect of Kat60 on adherens junction formation. p120ctn-RFP expression in FCs was imaged in control (top) and Kat60 (bottom) expressing trachea. Central signal correspond to FC contact site. Frame rate 18 sec. Original movie used to prepare Fig. 4i.
Data
Microtubule dynamics in tracheal FC and pAs revealed by tau-GFP marker expressed by the esg_FCGal4 driver. Original movie used to prepare Fig. 1d.
Data
Dynamics of myosin localization in DB fusion site revealed by esg_FC > GFP-Zip. Related to Fig. 3a.
Data
High-speed imaging of E-cadherin trafficking during the first 3 minutes after contact of FCs. Related to Fig. 2f.
Data
Laser perturbation of fusion cell contraction. Embryos carrying btl-Gal4, UAS-GFP-moe and UASp120ctn-RFP was illuminated with 1440 nm laser (60mW) for 1 second at one of fusion cells (left) of DB5 (t=0). The embryo was imaged with PlanApo 60x NA1.40 oil IR lens with 4x zoom. Then 512 x 512 pixel images of 1-mm-thick sections were captured every 30...
Data
Double labeling of EB1-GFP (green) and membrane marker (red) imaged in a btl > EB1-GFP, mRFPCAAX embryo. Dorsal branch was directed toward 2 o'clock direction. EB1 comet repeatedly entered into filopodia. Original movie used to prepare Fig. 4d. Scale bar: 10μm.