B.K. Kolita Kamal Jinadasa

National Aquatic Resources Research and Development Agency | NARA · Analytical Chemistry Laboratory

Mercury (Hg) is a global environmental pollutant that has been the cause of many public health concerns. It is transferred through trophic level and bio magnification in the food chain. Total Hg level was measured by cold vapour atomic absorption spectrometry in muscle tissue of 176 Swordfish (Xiphiasgladius) samples ranging from 11.8-112.0 kg tota...

Indian Backwater oysters (Crassostrea madrasensis) are available en mass around Sri Lanka but nutritional value and seasonal changes have not been studied sufficiently. Proximate composition and percentage edibility (PE) for wild and cultured oysters in Kala Oya estuary and Puttalam lagoon were studied. Influence of environmental factors on proxima...

The study was conducted to assess the health risks of selected metals in cultured and wild Crassostrea madrasensis in Sri Lanka over 13 months. Metal concentrations varied monthly, and Zn and Cu concentrations significantly varied between cultured and wild oysters. The mean metal concentrations (mg kg−1) were accumulated in order of Zn (1220.60) >...

Biogenic amines are basic nitrogen compounds, which are formed due to the decarboxylation of amino acids or/and the amination and the transformation of aldehydes and ketones. Histamine is a heterocyclic biogenic amine present in a wide range of food products, including fish and fishery products, meat and their products, and other dairy products and...

Alginates are natural polysaccharides that are extracted from brown seaweed varieties and it is widely used for their rheological properties. The main step in the extraction protocol of sodium alginate is alkaline extraction. Sodium alginate was produced by dipping the seaweed in 1% formaldehyde and 2.5% of sodium carbonate solution and properties...

The term “edible oils” (EO) refers to complex mixtures of lipids including main triacylglycerols (TAGs) or triglycerides, lower amounts of monoacylglycerols (MAGs), diacylglycerols (DAGs), phospholipids (PLs), free fatty acids (FAs), sterols and other minor components such as vitamins and antioxidants (Indelicato et al., 2017). They have several fu...

Ultrasound-Assisted Extraction (UAE) has attracted much attention in recent years due to its rapid extraction capability, high extraction efficiency, and being an environmentally friendly and low-cost methodology. This review gives a general overview of UAE and specifically its recent applications on fish and aquatic living resources. Moreover, the...

Mercury (Hg) is a well-known toxicant which enters the marine environment by both natural and anthropogenic sources. Consumption of fish and other seafood that contain methylmercury (MeHg) is a leading source of Hg exposure in humans. Considerable efforts have been made to mitigate the Hg presence and reduce its risks to humans. In this review the...

Fish and seafood are popularly consumed all over the world for their rich nutritional qualities and numerous health benefits. In this chapter, we present overview of the major arsenic (As) species found in the seafood, its accumulation pathway, toxicity, and health effects, regulation analytical techniques, and risk assessment. Further, the bioavai...

Fish and seafood are popularly consumed all over the world for their rich nutritional qualities and numerous health benefits. In this chapter, we present overview of the major arsenic (As) species found in the seafood, its accumulation pathway, toxicity, and health effects, regulation analytical techniques, and risk assessment. Further, the bioavai...

The Oyster Crassostrea madrasensis is abundant in Kala Oya estuary. This edible oyster is collected from both wild and cultures in Sri Lanka. As filter feeders, they are well known to accumulate toxic metals from the surrounding environment hence, quantifying nonessential trace metals and understanding spatial and temporal variations is essential....

This chapter discusses in detail the potential of several combined extraction and microextraction techniques for pretreating food waste. The discussion is focused on the combination of well-established methodologies such as ultrasound/microwave-assisted extraction and microwave-assisted acid digestion used for target extraction with preconcentratio...

The purpose of this investigation was to study the shelflife of spotted sardinella (Amblygaster sirm) fish stored in ice. During the storage period of 21 days, sensory, biochemical and microbial evaluation including pH, histamine, Total Volatile Base Nitrogen (TVB-N), Aerobic Plate Count (APC), Torry meter and sensory analysis were done at 3-day in...

Numerous studies have shown growing information indicating the contribution of food to the dietary exposure of arsenic (As) through consumption of different food items in many different regions over the world. However, few review papers with regard to As in Sri Lankan foods are available in databases. Thus, a critical review and assessment of a num...

Numerous studies have shown growing information indicating the contribution of food to the dietary exposure of arsenic (As) through consumption of different food items in many different regions over the world. However, few review papers with regard to As in Sri Lankan foods are available in databases. Thus, a critical review and assessment of a num...

Mercury (Hg) in fish and aquatic products is a potential threat to human health and international trade, and guidelines of international advisory bodies are established for assessing Hg in these foods. As fish are the most consumed animal protein in Sri Lanka, this assessment summarises for the first-time total mercury concentrations in Sri Lankan...

In the present study conducted for analysed the fatty acid composition of the Blue Swimmer Crabs (BSC) collected from Negombo, down south and Mannar coastal region in Sri Lanka. The fatty acid composition was analysed by Gas Chromatography (GC) techniques after preparation of the Fatty Acid Methyl Esters (FAME). The results showed that stearic acid...

In this study, muscle of blue swimmer crabs (Portunus pelagicus) were analysed for the content of trace metal contaminants. The crabs were collected at the landing sites along the coast of Mannar, Negombo and down south. Totally 60 individuals were collected during the period of May to November, 2014 and the samples were kept in ice and sent to the...

Mercury (Hg) and cadmium (Cd) are contaminants of great environmental concern due to their natural and anthropogenic origin, their ability of bioaccumulate through the food chain and their harmful effects on human health. In this study, the Hg and Cd accumulation in the muscle of 65 yellowfin tuna (Thunnus albacares) collected from two different ca...

The aim of this study was to compare the effect of three different culinary treatments (Chili curry, Coconut milk curry and Ambulthial) on the content of main Poly-Unsaturated Fatty Acids (PUFA) of three marine fish species namely, yellowfin tuna (Thunnus albacares), swordfish (Xiphias gladius) and spotted sardine (Amblygastersirm). After culinary...

A sensitive and widely applicable High-Performance Liquid Chromatography (HPLC) with fluorescence detector (FLD) method for determination hydrolysed amino acids in fish and aquatic products was developed and validated through study. The method involved a microwave-assisted sample extraction and protein hydrolysed with the hydrochloric acid and prop...

The marketplace contains a choice of both cultured and wild oysters, often subtle differences in taste and characteristics are observed between wild and cultured oysters. Therefore, seasonal variations of total lipid and total fatty acid compositions were studied in both, wild and cultured populations of Crassostrea madrasensis in Gangewadiya in Ka...

Background A total of 181 samples of cereals and legumes, including mustard, maize, finger millet, sesame, cowpea/black-eyed pea, Urad dal /split bean, foxtail millet, long bean and green gram from the North Central Province of Sri Lanka were analysed for arsenic (As) and cadmium (Cd) levels using atomic absorption spectrophotometry (AAS). Finding...

Commercially important marine fish, yellowfin tuna (YFT), and swordfish (SF), collected from the Indian Ocean around Sri Lanka were analysed for mercury (Hg) and cadmium (Cd) content. The ranges for Hg were < 0.07–1.60 and < 0.07–4.30 mg/kg, while for Cd < 0.006–0.134 and 0.006–0.180 mg/kg in YFT and SF, respectively. In SF 13.3% and in YFT 9.2% ex...

Mercury (Hg) and cadmium (Cd) are non-essential trace elements that transfer through the trophic chain which ultimately bio-accumulate and biomagnify in the upper trophic level. The total Hg (THg) and Cd levels of the muscle tissues of swordfish, Xiphias gladius caught in three different areas in the Indian Ocean around Sri Lanka were determined. T...

Total Diet Study (TDS) is a world-recognized approach in quantifying nutrients and contaminants in complete diets. In the present study, the TDS approach was used to determine the dietary exposure to mercury (Hg) and cadmium (Cd) by regular consumption of yellowfin tuna (YFT), swordfish (SWF) and sardinella (SAF) fish in Gampaha district, Sri Lanka...

Five tropical seaweeds (Sargassum wightii, Ulva lactuca, Sargussum turbinaria, Kappaphycus alvarezii, and Gracilaria verrucosa) found in the sea around Sri Lanka were evaluated for the nutritional value that was used as a complementary source of protein, fiber, ash, oil, essential and non-essential trace elements for human and animals. The moisture...

Blubberlip Snapper (BLS, Lutjanus rivulatus) are the reef-associated high commercial value fish species in Sri Lanka. Twenty amino acids (mg/kg) and seventeen fatty acids (% of the lipid fraction) were analyzed of the edible muscle portion in 18 individual BLS. The average length and weight of the female and male fish were significantly different (...

Consumption of contaminated fish is the major source of the human exposure to total mercury (T-Hg) and methyl mercury.Thisstudy reports the fish consumption habitsof three groups based on the age categoryand their occupation; “Children” (age 9-20 years) and two groups of “Adults” (age above 21 years) based on their occupation; “Adults-Fishermen”(fi...

Background: Quantification of toxic trace metals in biota is very important to monitor both ecosystem health and public health. Stomopneustes variolaris is a widespread species of sea urchin in the world that have edible gonads that are in high demand. This study attempted to examine the levels of accumulated toxic trace metals and their relation-...

Sodium (Na) and potassium (K) are essential trace minerals, plays an important role in health and disease conditions, and studies about them have been attracting significant interest. The aim of the present study was to assess the Na and K concentration in selected common food samples available in the Sri Lankan market. Sri Lankan consumes too much...

The edible oyster Crassostrea madrasensis is naturally found in Kala-oya estuary and the presence of tourist industry has created small scale culture based fisheries within coastal communities. But little attention is paid towards the studies on nutritional composition of oysters. Monthly variation of total lipid and fatty acid composition were det...

Twenty organochlorine pesticide residues (OCP) were analysed in the muscle tissue of the seven selected fish species (n=42) known as Tilapia (Oreochromis sp.), Striped snakehead (Channa striata), Eel (Anguilla sp.), Pearl spot (Etroplus suratensis), Long whiskers catfish (Mystus gulio), Stinging catfish (Heteropneustes fossilis), Zigzag eel (Mastac...

The thermal effects on nutritional quality of two species of squids (Loligo duvauceli, Sepioteuthis lessoniana) and cuttlefish (Sepia latimanus, Sepiella inermis) were investigated before and after thermal processing. Proximate composition and energy values were analysed according to AOAC official procedures. The moisture content of all fresh fille...

Dried fish is one of the traditionally preserved foods in Sri Lanka.Since dried fish is often an important component of daily meal, this study was aimed to assess the quality of selected dried fish varieties in the local market. Dried fish samples of nine selected varieties were collected under three categories, viz. locally produced, imported drie...

This study attempted to describe the length and weight frequency, and length-weight relationship in the black sea urchin, Stomopneustes variolaris, in Sri Lanka. The sampling sites Mount-Lavinia (n=43), Beruwala (n=99) and Tangalle (n=55) were selected from South-west coast in Sri Lanka. The shell length and body weight were measured separately for...

Sri Lanka is a developing country in the South Asian region of the world. In the Country's efforts in the overall development of its people, dietary surveys are essential to estimate nutrient intake, as it plays a major role in the health and well being leading to productivity. Sugars are a class of carbohydrates and thus one of the most common ele...

A high-performance liquid chromatography method is described for quantitative determination and validation of histamine in fish and fishery product samples. Histamine is extracted from fish/fishery products by homogenizing with tri-chloro acetic acid, separated with Amberlite CG-50 resin and C18-ODS Hypersil reversed phase column at ambient tempera...

Sea urchin samples (Stomopneustes variolaris) were collected from the selected (3 sampling locations) western and southern coastal area around the Sri Lanka (n=193) from the time period of May to October, 2014. Sampled animals were measured for biometrical parameters, dissected and separated roe tissues, which were analysed for the proximate compos...

Skipjack tuna samples (n= 44) were collected from representative fish landing sites around Sri Lanka. Proximate composition, fatty acid profile and major essential trace minerals were analyzed according to their respective standard methods, strictly adhering with quality control procedures. As per the results, Skipjack tuna is a rich source of prot...

Peradeniya Aglinic acid is a hydrocolloid of a polysaccharide origin, which is extracted from brown seaweeds. It is commonly used as a stabilizer, thickener, and a gelling agent in a food industry, and also as a matrix material in pharmaceuticals. The objectives of this study were to develop a method for the extraction of alginic acid from Sargussu...

Peradeniya Shark (Carcharhinus falciformis) is one of the most abundant shark species in the pelagic zone around the world and accounts for more than 60% of total shark landings in Sri Lanka. Essential trace elements; iron (Fe), copper (Cu), and zinc (Zn) and non-essential trace elements; lead (Pb), cadmium (Cd), mercury (Hg) and arsenic (As), foun...

This study aimed to investigate quality of fish landed in Negombo area and distributed in suburban areas in Western province of Sri Lanka. Hundred samples of large fish (Katsuwonus pelamis and Euthynnus affinis) and 60 samples of small fish (Amblygaster sirm, Pterocaesio chrysozona, Stolephorus commersoni, and Sardinella albella) were sampled from...

Oyster fishery is one of the emerging small-scale fishery among poor coastal communities in Sri Lanka. However, oysters are highly esteemed seafood and considered a delicacy throughout the world, as they constitute a rich source of many of the elements, essential for providing a balanced diet. Nutrition content of bivalves can be changed according...

Mercury (Hg) is a naturally occurring metal in the earth's crust and can enter the aquatic environment through natural and anthropogenic activities. Part of Hg is converted to methyl-mercury (MeHg) and accumulates in fish through the food chain reaching its highest levels in large predatory fish such as tuna. Consumption of contaminated fish has be...

Mercury (Hg) is a naturally occurring metal in the earth's crust and can enter the aquatic environment through natural and anthropogenic activities. Part of Hg is converted to methyl-mercury (MeHg) and accumulates in fish through the food chain reaching its highest levels in large predatory fish such as tuna. Consumption of contaminated fish has be...

The aim of the current study was to determine the Quality Index of skipjack tuna th day of ice storage. In the current study, total aerobic plate count of skipjack tuna reached the 10 8 CFU/g levels, which indicates the initiation of spoilage on day 9. The 12 days shelf life = 0.96)

Sea urchins

A major challenge in commercial catfish farming is to feed the fish to achieve optimal growth with minimal waste. Protein and lipids play a major role in a fish feed as fish gathers energy through these high cost ingredients. Dentification of the optimal protein and lipid combination is important to buildup profitable aquaculture industry. The effe...

The present study was carried out to assess the health benefits vs. risks of consuming commercially important fish skipjack tuna (Katsuwonus pelamis) in Sri Lanka. Skipjack tuna samples (n=44) collected from fish landing sites around Sri Lanka were analysed for proximate composition by AOAC, 2000 standard methods: Fatty acids composition, histamine...

Abstract A major challenge in commercial catfish farming is to feed the fish to achieve optimal growth with minimal waste. Protein and lipids are playa major role in a fish feed as fish gathers energy through these high cost ingredients. Identification of the optimal protein and lipid combination is important to buildup profitable aquaculture indus...

Introduction Thailand catfish Pangasius sutchi is most commonly used as ornamental fish in Sri Lanka. Catfish were imported to Sri Lanka as they were difficult to breed successfully in Sri Lanka under the natural environmental conditions. But recent studies have found that Thailand catfish can be bred artificially in Sri Lanka. There is a considera...

The effects of dried powder of Amazon sail fin catfish (tank cleaner (TC)), (Pterygoplichthys pardalis) as a protein supplement in feeds of cage-cultured fish, seabass (Lates calcarifer) were studied in Negombo lagoon for 72 days. Three isonitrogenous (42.17±0.32%) and isocaloric (4306.15±58 cal g-1) diets were prepared by using TC powder and loca...

Currently, an aquatic ecosystem of Sri Lanka is faced with acute environmental problems due to trace metal pollution. Bomburuella is one of reservoirs situated in Nuwara-Eliya District, Sri Lanka. Trace metal pollution of this reservoir has taking placed due to various anthropogenic activities. Therefore, it is very important to determine the cause...

This study was conducted to investigate the effect of storage temperatures on the shelf life of yellowfin tuna (YFT) loins by studying of microbial, chemical and organoleptic changes. Shelf life of YFT was determined through changes in total aerobic bacterial plate counts (APC), total volatile basic nitrogen (TVB-N) trimethylamine (TMA), organolept...

This study was carried out to evaluate the quality of five marine fishes yellowfin tuna (Thunnus albacores, Bonnaterre, 1788), sailfish (Istiophorus platypterus, Shaw, 1792), slaya/sardine (Sardinella gibbosa, Bleeker, 1849), squid (Loligo duvaucelii, D' Orbigny, 1835) and shrimp (Fenneropenaeus indicus, H. Milne Edwards, 1837) based on total volat...