Björn Nitzsche

Björn Nitzsche
University of Leipzig · Department für Nuklearmedizin

DVM, PhD

About

38
Publications
4,544
Reads
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1,481
Citations
Citations since 2016
19 Research Items
710 Citations
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2016201720182019202020212022020406080100
2016201720182019202020212022020406080100
2016201720182019202020212022020406080100
Introduction
Computational neuroscience in large gyrencephalic animal stroke model to understand diffusion and perfusion features of stroke development.
Additional affiliations
August 2015 - present
University of Leipzig
Position
  • PostDoc Position
Description
  • Large (such as sheep) and small (rodents, rabbits) animal models of stroke including several image modalities (DCW-MRI, pASL,structuralMRI) processing, analysis,experimental neurosurgery, neuropathology (poststroke dementia), neurological deficits
November 2013 - September 2014
Sonovum AG
Position
  • Researcher
February 2012 - October 2013
Sonovum AG
Position
  • CSO
Education
April 2007 - January 2012
University of Leipzig
Field of study
  • Veterinary Medicine, Pathology
October 2001 - March 2007
University of Leipzig
Field of study
  • Veterinary Medicine
June 1996 - January 2000
ASTA Medica AG
Field of study
  • Biology Laborant

Publications

Publications (38)
Article
Full-text available
The surfactant protein-G (SP-G) has recently been discovered in the brain and linked to fluid balance regulations. Stroke is characterized by impaired vessel integrity, promoting water influx and edema formation. The neurovascular unit concept (NVU) has been generated to cover not only ischemic affections of neurons or vessels but also other region...
Article
Translation of acute ischemic stroke research to the clinical setting remains limited over the last few decades with only one drug, recombinant tissue-type plasminogen activator, successfully completing the path from experimental study to clinical practice. To improve the selection of experimental treatments before testing in clinical studies, the...
Article
Selective therapeutic hypothermia (TH) showed promising preclinical results as a neuroprotective strategy in acute ischemic stroke. We aimed to assess safety and feasibility of an intracarotid cooling catheter conceived for fast and selective brain cooling during endovascular thrombectomy in an ovine stroke model. Transient middle cerebral artery o...
Article
Full-text available
Ischemic stroke causes cellular alterations in the “neurovascular unit” (NVU) comprising neurons, glia, and the vasculature, and affects the blood-brain barrier (BBB) with adjacent extracellular matrix (ECM). Limited data are available for the zone between the NVU and ECM that has not yet considered for neuroprotective approaches. This study descri...
Chapter
The human brain receives approximately 15% of the cardiac output and therefore is the most demanding organ in respect to blood flow supply. This fact emphasizes the importance of perfusion as a key factor in a variety of cerebrovascular and other diseases including stroke, migraine, and brain tumors. Today, numerous imaging techniques are able to v...
Article
Full-text available
Gut integrity impairment leading to increased intestinal permeability (IP) is hypothesized to be a trigger of critically illness. Approximately 15-20% of human ischemic stroke (IS) victims require intensive care, including patients with impaired level of consciousness or a high risk for developing life-threatening cerebral edema. Local and systemic...
Article
Gut integrity impairment leading to increased intestinal permeability (IP) is hypothesized to be a trigger of critically illness. Approximately 15–20% of human ischemic stroke (IS) victims require intensive care, including patients with impaired level of consciousness or a high risk for developing life-threatening cerebral edema. Local and systemic...
Data
Announcement for launching http://animal-brain-mapping.com. Magnetresonance-Tomographic-Imaging (MRI) of sheep and dogs brain are of utmost interest in clinical routine and neuroscientific research. However, post-processing of canine or ovine MR images are complicated due to rarely available standards. We have established templates, tissue proba...
Article
Intracerebral hemorrhage (ICH) is an important stroke subtype, but preclinical research is limited by a lack of translational animal models. Large animal models are useful to comparatively investigate key pathophysiological parameters in human ICH. To (i) establish an acute model of moderate ICH in adult sheep and (ii) an advanced neuroimage proces...
Article
Full-text available
As part of the neuronal cytoskeleton, neurofilaments are involved in maintaining cellular integrity. In the setting of ischemic stroke, the affection of the neurofilament network is considered to mediate the transition towards long-lasting tissue damage. Although peripheral levels of distinct neurofilament subunits are shown to correlate with the c...
Article
Stereotaxic systems and automatic tissue segmentation routines enable neuronavigation as well as reproducible processing of neuroimage datasets. Such systems have been developed for humans, non-human-primates, sheep, and rodents, but not for dogs. Although dogs share important neurofunctional and -anatomical features with humans, and in spite of th...
Article
Full-text available
As part of the extracellular matrix (ECM), perineuronal nets (PNs) are polyanionic, chondroitin sulfate proteoglycan (CSPG)-rich coatings of certain neurons, known to be affected in various neural diseases. Although these structures are considered as important parts of the neurovascular unit (NVU), their role during evolution of acute ischemic stro...
Article
Full-text available
Interspecies differences, anatomical and physiological aspects as wells as simplified study designs contribute to an overestimation of treatment effects and limit the transferability of experimental results into clinical applications. Confounders of cell therapies for cerebrovascular disorders (CVD) include common CVD comorbidities, frequent medica...
Article
Full-text available
Background Disease models are useful for prospective studies of pathology, identification of molecular and cellular mechanisms, pre-clinical testing of interventions, and validation of clinical biomarkers. Here, we review animal models relevant to vascular cognitive impairment (VCI). A synopsis of each model was initially presented by expert practi...
Poster
Anantomia Histologia Embryologia Supplements ISSN 1439-0264 joint congress of EAVA (European Association of Veterinary Anatomists) and WAHVM (World Association for the History of Veterinary Medicine) Introduction: Neuroscientific large animal models pose advantages over rodents including the application of complex imaging-based in vivo investigatio...
Article
Anantomia Histologia Embryologia Supplements Proceeding of joint congress of EAVA (European Association of Veterinary Anatomists) and WAHVM (World Association for the History of Veterinary Medicine) ISSN 1439-0264 Introduction: Stereotaxic references and brain atlases play a key role in neuroscientific research enabling objective and comparable...
Chapter
According to the recommendation of international expert committees, large animal stroke models are demanded for preclinical research. Based on a brief introduction to the ovine cranial anatomy, a sheep model of permanent middle cerebral artery occlusion (MCAO) will be described in this chapter. The model was particularly designed to verify several...
Conference Paper
Full-text available
International academic and industrial expert consortia (STAIR, STEPS) suggest stringent preclinical research strategies for stroke including validation of novel treatment strategies in gyrencephalic animal models. However, many existing gyrencephalic animal models are expensive, constrained by ethical considerations or limited by species-specific a...
Article
Full-text available
Standard stereotaxic reference systems play a key role in human brain studies. Stereotaxic coordinate systems have also been developed for experimental animals including non-human primates, dogs and rodents. However, they are lacking for other species being relevant in experimental neuroscience including sheep. Here, we present a spatial, unbiased...
Conference Paper
Full-text available
Objective: Sheep play an important role in studies of neuroscience including imaging procedures, neuronavigation and electrophysiology due to the large brain size and body weight of this gyrencephalic species. The anatomical and vascular comparability to human being emphasizes the importance for ovine models of cerebrovascular diseases such as isch...
Article
Full-text available
Cerebrovascular diseases are significant causes of death and disability in humans. Improvements in diagnostic and therapeutic approaches strongly rely on adequate gyrencephalic, large animal models being demanded for translational research. Ovine stroke models may represent a promising approach but are currently limited by insufficient knowledge re...
Chapter
Full-text available
Assessment of biodistribution and monitoring of cell migration processes in vivo are essential for the safety of novel cell-based therapies for ischemic stroke and early-stage clinical trials, but are mainly lacking investigation in large animal models which are closer to the situation found in human patients. This chapter reports a series of exper...
Article
Stroke is the third most common cause of death in industrialized countries. The main therapeutic target is the ischemic penumbra, potentially salvageable brain tissue that dies within the first few hours after blood flow cessation. Hence, strategies to keep the penumbra alive until reperfusion occurs are needed. To study the effect of inhaled nitri...
Article
Full-text available
Translational researchers and clinicians recommend the use of large animal models in preclinical stroke research. This represents an important part of a strategy aiming to prevent past translational failures in future therapeutic developments. Thirty-five Merino rams were subjected to sham surgery (n = 3), one-branch middle cerebral artery occlusio...
Article
Full-text available
We aimed to evaluate the feasibility and efficacy of autologous umbilical cord blood mononuclear cell (UCMNC) transplantation on right ventricular (RV) function in a novel model of chronic RV volume overload. Four-month-old sheep (n = 20) were randomized into cell (n = 10) and control groups (n = 10). After assessment of baseline RV function by the...
Article
Full-text available
As effective stroke treatment by thrombolysis is bound to a narrow time window excluding most patients, numerous experimental treatment strategies have been developed to gain new options for stroke treatment. However, all approaches using neuroprotective agents that have been successfully evaluated in rodents have subsequently failed in clinical tr...
Article
Full-text available
A key challenge of functional genomics today is to generate well-annotated data sets that can be interpreted across different platforms and technologies. Large-scale functional genomics data often fail to connect to standard experimental approaches of gene characterization in individual laboratories. Furthermore, a lack of universal annotation stan...

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Projects

Projects (2)
Project
Project aims include: I. The creation of standard references (template, TPM, atlas) enabling comprehensible image processing, statistical image analysis and reporting of findings in the sheep brain II. The volumetric and spatial determination of cerebral age and sexual dimorphism within a healthy population of sheep according to atlas space III. Specific adaptation of the references (see I) to enable observer independent automatic analysis of large lesioned brain tissue IV. Volumetric and spatial effects of infarct development on remaining cortical, subcortical, ventricular structures in subacute and chronic altered sheep brain after middle cerebral artery occlusion
Archived project
Stereotaxic labeling of structural cortical and subcortical areas in ovine brain