Bipasha Bhattacharjee

Bipasha Bhattacharjee
University of Kentucky | UKY · College of Agriculture

Doctor of Philosophy
Looking for collaborators in various domains in plant sciences and technical writing.

About

5
Publications
24,750
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
44
Citations
Introduction
Doctorate in Biological Sciences from @ AcSIR; Working on plant-virus interactions and development of host resistance to viral stress; DST-INSPIRE SRF-PhD (Gold medallist) @ CSIR-IHBT; NET 2016; Fulbright Doctoral Fellow to University of Kentucky, Lexington, USA 2020-2021
Additional affiliations
August 2014 - May 2015
North Eastern Hill University
Position
  • DBT-NER studentship
Education
August 2020 - August 2021
University of Kentucky
Field of study
  • Plant microbe interactions under Nehru-Fulbright Doctoral Fellowship

Publications

Publications (5)
Article
Full-text available
Nuclear factor-Y (NF-Y) transcription factors (TFs) are conserved heterotrimeric complexes present and widespread across eukaryotes. Three main subunits make up the structural and functional aspect of the NF-Y TFs: NF-YA, NF-YB and NF-YC, which bind to the conserved CCAAT- box of the promoter region of specific genes, while also interacting with ea...
Chapter
Plants get exposed to various types of microorganisms in real time, which positively or negatively regulates their growth and development. On contact or sensing foreign biotic agents, intricate molecular and physiological changes are triggered within the plant system, which leads to the activation of a host of pathways relating to defense and morph...
Article
Full-text available
A persistent issue in the agricultural sector worldwide is the intensive damage caused to crops by the geminivirus family of viruses. The diverse types of viruses, rapid virus evolution rate, and broad host range make this group of viruses one of the most devastating in nature, leading to millions of dollars’ worth of crop damage. Geminiviruses hav...
Article
Plants deploy RNA silencing as a natural defence against invading viruses involving sequence-specific degradation of the viral RNAs. As a counter-defence strategy, viruses encode suppressor proteins that simultaneously target different steps of the silencing machinery. Tomato leaf curl Palampur virus (ToLCPalV) is a bipartite begomovirus in Geminiv...
Article
Chitinases are varied sized proteins which have the ability to degrade chitin and are present in a huge range of organisms like fungi, yeasts, arthropods, humans etc. and have been getting increased attention due to their biocontrol properties. In silico analysis sheds light on the extensive properties of this plant protein. In this paper, a partic...

Questions

Questions (25)
Question
I have a comparative transcriptome dataset, with a number of upregulated and downregulated genes. I want to construct a protein-protein interactome network to understand the node and hub genes and modules. Should just the upregulated DEGs be taken for the same or total?
Question
Could anyone offer an alternative to the GRG-X? Have been trying to use it but failing without the adobe flash player!
Question
Hi all,
I have been trying to use the GAL4 system of Y2H for checking interactions between plant proteins and viral proteins. The protocol that I use is the Clontech manual protocol and the yeast strain is AH109 and I use it for co-transformation. We have the genes cloned in PGBKT7 and PGADT7. We streak the AH109 in YPDA plates supplemented with adenine and transform the yeast using YPD broth + adenine. However, after plating the co-transformants in SD-LT plates, we get very poor transformants and even the transformed control plasmids do not seem to be growing on the plates. We use the TE/LiAc protocol. Inputs needed! Thank you
Question
I have been having a strange issue growing AH109 and using it for transformation. Usually, I would plate it in YPDA and culture it at YPDA too, the generic protocol that is used for a two-hybrid transformation. However, as I plate it in -LT plates, I get a vigorous growth of pink coloured yeast. I have been unable to get the white colonies at all for some time and I have changed bottles of media, amino acid dropouts and even laminar airflows. I am attaching pictures of the same. I even tried transferring them in -alth x@gal plates but there too I got pink growth. Please help!
Question
Hi all,
I have been using YPDA from some time now to grow yeast, but I haven't been getting yeast growth in 3-4 days. The colonies that appear are tiny and when I am doing head to head interaction studies, they grow poorly on SD-alth x-a-gal media. I have stopped using YPD because the yeast growth in SD-alth is so not good too. Please let me know what I can do. I had only once had very good growth on YPDA with 3-4mm size yeast colonies, but the ones that are growing now are slow and very small, even though stock is same!
Question
I have two arabidopsis mutants of SALK and Wisconsin Lox. I genotyped them with LP-RP primers and the LBB and P745 primers to check the homozygous lines. When I've isolated the RNA from it and used the cDNA to run a PCR with the same LP RP primers, I'm getting an amplification of two bands in wild type columbia and a single band in all the TDNA mutants. Can anyone help me figuring out the results?
Question
I am transforming yeast grown on YPDA media and then plating them on SD/-Trp/-Leu. After obtaining the transformants, I dilute the colonies in water and plate on SD/-Trp/-Leu/-His /-Ade X-@ Gal media. While I am able to get the blue coloration on positive controls, the yeast does not seem to grow at all and cannot be photographed nor can infer on the interaction properly. It is just a blue translucent type growth. I use 20mg/ml x-@ Gal after autoclaving. The SD media is pH checked. Please give your opinions!
Question
I have two constructs of arabidopsis genes cloned in pet28 vector, transformed in rosetta. The glycerol stocks made three years back were streaked in kan plates, single colony checked with pcr and confirmed and induced using general protocol ( primary culture of 5ml, secondary culture of 5ml-OD 0.8, induction of 4ml secondary culture with 1mM IPTG for 4hours at 37°C, sonication in 1X PBS, sample processing with loading dye at 100°C for 5min, ice for 5min and loading in PAGE gel). Unfortunately the profile of induced and uninduced are exactly same as run in gel. The person who had cloned it 3 years back was able to induce the protein in the supernatent with the very same conditions. Can anyone point out what wrong could I be doing? I'm attaching a gel image representative of the kind of result that I'm seeing.
Thanks a ton.
Question
So, I have been cloning a lot of genes over the years in various plant transformation and overexpression vectors and even though its a struggle always to generate positive clones, I usually am able to confirm the sequences and use them for expression studies.
I am now cloning a truncated plant TF in the Tobacco Rattle virus (TRV2) system after confirming its clone in PgemT Easy cloning vector and have been failing from the past three months. I have used to sites NcoI and Xho1 for directional cloning, the Promega rapid ligation system and have kept for ligation at various time intervals ranging from short to overnight ligation, using minimum 50ng vector to 150 ng in concentration at a ratio of 1:1,2:1,3:1 and 5:1. However, on using TRV2 specific primers I am only getting amplification of the vector at about 150bp instead of getting about 450bp amplification (insert+vector). Please suggest me few tips.
Question
Hi all, I need an info on the CABS flex and how it works for protein protein interaction simulations. I have docked structures obtained using cluspro and would like to subject them to MD. Please give me an insight on it if anyone has used it.

Network

Cited By