Bin Cao

Bin Cao
Nanyang Technological University | ntu · School of Civil and Environmental Engineering

B.Eng; Ph.D.

About

120
Publications
31,809
Reads
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4,582
Citations
Citations since 2016
62 Research Items
3900 Citations
20162017201820192020202120220200400600800
20162017201820192020202120220200400600800
20162017201820192020202120220200400600800
20162017201820192020202120220200400600800
Additional affiliations
September 2017 - present
Nanyang Technological University
Position
  • Professor
December 2011 - present
Singapore Centre on Environmental Life Sciences Engineering
Position
  • Principal Investigator
December 2011 - August 2017
Nanyang Technological University
Position
  • Professor

Publications

Publications (120)
Article
Full-text available
Quorum quenching (QQ) has been reported to be a promising approach for membrane biofouling control. Entrapment of QQ bacteria in porous matrices is required to retain them in continuously operated membrane processes and to prevent uncontrollable biofilm formation by the QQ bacteria on membrane surfaces. It would be more desirable if the formation a...
Article
The interaction between biofilms and disinfectant in drinking water distribution systems (DWDS) as well as the role of this interaction in the formation of disinfection byproducts (DBPs) has been extensively studied in recent years. In contrast, lysis of cells and/or release of intracellular biomolecules from inactivated/damaged cells and their fat...
Article
In membrane biofouling studies, quantification of biofouling is often conducted destructively and the results reflect only a snapshot of the biofouling processes. This limitation is mainly due to the lack of tools that allow us to monitor dynamics of biofouling without the need to disassemble the membrane testing systems. In this study, we develope...
Article
Full-text available
Importance: Bioaugmentation is a bioremediation approach that has been often described in literature but has been hardly to no successfully applied in practice. Many biological and environmental factors influence the overall performance of bioaugmentation. Among which, the interaction between the target pollutant and the augmented bacterial cells...
Article
Comamonas is one of the most abundant microorganisms in biofilm communities driving wastewater treatment. Little has been known about the role of this group of organisms and their biofilm mode of life. In this study, using Comamonas testosteroni as a model organism, we demonstrated the involvement of Comamonas biofilms in denitrification under bulk...
Article
Monochloramine (MCA) is a widely used secondary disinfectant to suppress microbial growth in drinking water distribution systems. In monochloraminated drinking water, a significant amount of extracellular DNA (eDNA) has been reported, which has many implications ranging from obscuring DNA-based drinking water microbiome analyses to posing potential...
Article
Urease is a dinickel enzyme commonly found in numerous organisms that catalyses the hydrolysis of urea into ammonia and carbon dioxide. The microbially induced carbonate precipitation (MICP) process medicated by urease-producing bacteria (UPB) can be used for many applications including, environmental bioremediation, soil improvement, healing of cr...
Article
Environmental DNA, i.e., DNA directly extracted from environmental samples, has been applied to understand microbial communities in the environments and to monitor contemporary biodiversity in the conservation context. Environmental DNA often contains both intracellular DNA (iDNA) and extracellular DNA (eDNA). eDNA can persist in the environment an...
Article
This review discusses the latest trend in recovering valuable metals such as lithium, cobalt, nickel, and manganese from spent lithium-ion batteries (LIBs) to avoid the technological world's critical metal demands. LIBs are a secondary source of valuable metals such as Li (5%-7%), Ni (5%-10%), Co (5%-25%), Mn (5-11%), and non-metal graphite. Recycl...
Article
Monochloramine (MCA) is often used as a secondary disinfectant in drinking water distribution systems (DWDS) for controlling the regrowth of microorganisms. Laboratory experiments to study the efficacy of MCA and formation of disinfection byproducts in DWDS all require the preparation of MCA. Different protocols for the preparation of MCA have been...
Article
Soil, a potential reservoir of antibiotic resistance genes (ARGs), is inhabited by numerous microorganisms. Many microorganisms in soil are embedded within a self-produced matrix of extracellular polymeric substances to form supracellular structures, i.e., biofilms. Representing the predominant microbial lifestyle in soil, soil biofilms are conside...
Article
Spent lithium-ion batteries (LIBs) are more hazardous due to the presence of several toxic metals such as cobalt, lithium, nickel, manganese, etc. as well as electrolytes such as LiPF6, LiBF4, or LiClO4. However, these spent LIBs are the secondary source of metals that can be extracted and reused in many ways to decrease their potential environment...
Article
Most studies on the diversity of magnetotactic bacteria (MTB) have been conducted on samples obtained from the Northern or the Southern hemispheres. The diversity of MTB in tropical Asia near the geo-equator, with a close-to-zero geomagnetic inclination, weak magnetic field and constantly high seawater temperature has never been explored. This stud...
Article
The genus Shewanella comprises about 70 species of Gram-negative, facultative anaerobic bacteria inhabiting various environments, which have shown great potential in various biotechnological applications ranging from environmental bioremediation, metal(loid) recovery and material synthesis to bioenergy generation. Most environmental and energy appl...
Article
Full-text available
Electrochemically active biofilms are capable of exchanging electrons with solid electron acceptors and have many energy and environmental applications such as bioelectricity generation and environmental remediation. The performance of electrochemically active biofilms is usually dependent on c-type cytochromes, while biofilm development is control...
Article
Compared with the chemically defined synthetic wastewater (SynWW), real wastewater has been reported to exhibit distinct effects on microbial community development. Whether and how soluble microbial products in real wastewater contribute to different effects of synthetic and real wastewater on the fate of exogenous bacteria remains elusive. In this...
Article
Biocement based on microbially induced carbonate precipitation has become a new construction material for soil cementation. One of the key elements of biocement is urease-producing bacteria (UPB). This paper presents a new method to enrich UPB from waste activated sludge (WAS) under a non-sterile condition so the cultivation of bacteria can be carr...
Article
In green chemical synthesis, biofilms as biocatalysts have shown great promise. Efficient biofilm‐mediated biocatalysis requires the modulation of biofilm formation. Optogenetic tools are ideal for controlling biofilms, as light is non‐invasive, easily controllable and cost‐efficient. In this study, we employed a near infrared (NIR) light‐responsiv...
Article
In this study, we report an interdisciplinary and novel strategy towards biofilm engineering for the development of biofilm-templated heteroatom-doped catalytic system through bioreduction and biofilm matrix-facilitated immobilization of the in situ formed catalytic nanoparticles followed by controlled pyrolysis. We showed that (i) even under room...
Article
Full-text available
Re-naturalized quarry lakes are important ecosystems, which support complex communities of flora and fauna. Microorganisms associated with sediment and water form the lowest trophic level in these ecosystems and drive biogeochemical cycles. A direct comparison of microbial taxa in water and sediment microbial communities is lacking, which limits ou...
Article
Full-text available
Quorum quenching (QQ) has been reported to be a promising approach for membrane biofouling control. Entrap-ment of QQ bacteria in porous matrices is required to retain them in continuously operated membrane processes and to prevent uncontrollable biofilm formation by the QQ bacteria on membrane surfaces. It would be more desirable if the formation...
Article
Full-text available
Comamonas is often reported to be one of the major members of microbial communities in various natural and engineered environments. Versatile catabolic capabilities of Comamonas have been studied extensively in the last decade. In contrast, little is known about the ecological roles and adaptation of Comamonas to different environments as well as t...
Article
Full-text available
This study investigated the metabolism of Pseudomonas aeruginosa PAO1 during its biofilm development via microscopy imaging, gene expression analysis, and 13C-labeling. First, dynamic labeling was employed to investigate glucose utilization rate in fresh biofilms (thickness 40∼60 micrometer). The labeling turnover time of glucose-6-P indicated biof...
Article
Understanding the dynamics of biofilm development in response to chemical cues and signals is required toward the development of controllable biofilm‐mediated bioprocesses. In this study, we report a new biofilm growth system that integrates a microfluidic gradient mixer with a biofilm growth chamber. The biofilm growth system allows biofilms grow...
Article
Spent Li-ion batteries (LIB) are highly rich in Cobalt and Lithium that need to be recovered to reduce shortage of these valuable metals and decrease of their potential environmental risks. This study applied bioleaching using Aspergillus niger strains MM1 and SG1, and Acidithiobacillus thiooxidans 80191 for removal of Co and Li from spent LIB unde...
Article
Full-text available
Geopolymer is an emerging alternative green binder to Portland cement. Geopolymer is often more brittle and thus it is highly desirable to impart self-healing into geopolymer. Unlike Portland cement concrete, self-healing through hydration of cement, and leaching and carbonation of hydration products are not feasible in geopolymer. Microbially indu...
Article
Ultraviolet-A (UVA) (365 nm) irradiation with intensity of solar irradiance on a sunny day was detrimental to the culturability of Enterococcus faecalis in stormwater matrices. The effect was mitigated by...
Article
Full-text available
Although biocatalytic transformation has shown great promise in chemical synthesis, there remain significant challenges in controlling high selectivity without the formation of undesirable byproducts. For instance, few attempts have been successful in constructing biocatalyst for de novo synthesis of pure flavin mononucleotide (FMN) due to riboflav...
Article
Full-text available
The efficient handling of wastewater pollutants is a must, since they are continuously defiling limited fresh water resources, seriously affecting the terrestrial, aquatic, and aerial flora and fauna. Our vision is to undertake an exhaustive examination of current research trends with a focus on nanomaterials (NMs) to considerably improve the perfo...
Article
We developed a facile approach to synthesize thin-film nano-templated composite (TFNt) nanofiltration membrane with high water permeability, high NaCl/MgSO4 selectivity and strong antimicrobial properties. A polydopamine (PDA) coating on a polysulfone support was used as a nano-template to generate silver nanoparticles (AgNPs) in situ with high loa...
Article
Die Beschichtung einzelner Bakterienzellen mit in situ gebildetem Polypyrrol wird von J. S. C. Loo, B. Cao, J. R. Zhang, J. J. Zhu et al. in ihrer Zuschrift (DOI: 10.1002/ange.201704729) beschrieben. Das Polypyrrol kann als leitfähiges Medium sowie als Schutzschicht fungieren. Ein erhöhter direkter kontaktbasierter extrazellulärer Elektronentransfe...
Article
Coating individual bacterial cells with in situ formed polypyrrole is described by J. S. C. Loo, B. Cao, J. R. Zhang, J.-J. Zhu et al. in their Communication on page 10516 ff. The coated polypyrrole can serve as a conductive medium as well as a protecting layer. Enhanced direct contact-based extracellular electron transfer and improved viability of...
Article
In this study, we developed a two-dimensional mathematical model to predict substrate utilization and metabolite production rates in Shewanella oneidensis MR-1 biofilm in the presence and absence of uranium (U). In our model, lactate and fumarate are used as the electron donor and the electron acceptor, respectively. The model includes the producti...
Article
Coating individual bacterial cells with conjugated polymers to endow them with more functionalities is highly desirable. Here, we developed an in situ polymerization method to coat polypyrrole on the surface of individual Shewanella oneidensis MR-1, Escherichia coli, Ochrobacterium anthropic or Streptococcus thermophilus. All of these as-coated cel...
Article
Full-text available
To prolong the lifespan of landfills and to recover waste into resources, it is of great interest to develop environment-friendly and sustainable routes for municipal solid waste (MSW) incineration bottom ash (IBA) recycling and utilization. In this study, mesoporous materials were hydrothermally synthesized from incineration bottom ash. The proper...
Article
Full-text available
We report a facile method for preparing silver-loaded membranes for point-of-use disinfection and disaster relief applications. A bio-inspired material, polydopamine, was coated onto a highly porous nanofibrous polyacrylonitrile substrate. We then take advantage of the redox properties of polydopamine to form silver nanoparticles in situ. These nan...
Article
Wastewater treatment using forward osmosis anaerobic membrane bioreactors (FO-AnMBR) has a number of advantages over traditional wastewater treatment approaches. Previous studies have investigated the overall performance of lab-scale FO-AnMBR in treating synthetic wastewater. Due to enhanced physical rejection of osmosis membrane, salinity graduall...
Conference Paper
Full-text available
The presence of endocrine disrupting compounds (EDCs) such as Ethinylestradiol (EE2) in the aquatic environments have a significant impact on the mechanism and the function of the endocrine system of human health and wildlife, e.g., fishes, mammals, birds, reptiles, amphibians, and invertebrates.1-2 The US Food and Drug Administration (FDA) and the...
Article
Full-text available
Clay minerals and metal oxides, as important parts of the soil matrix, play crucial roles in the development of microbial communities. However, the mechanism underlying such a process, particularly on the formation of soil biofilm, remains poorly understood. Here, we investigated the effects of montmorillonite, kaolinite, and goethite on the biofil...
Article
A novel, biofilm-based AND logic gate was constructed in Shewanella oneidensis through a near-infrared (NIR) light responsive c-di-GMP module. The logic gate was demonstrated in microbial fuel cells with isopropyl β-D-thiogalactoside (IPTG) and NIR light as the inputs and electrical signals as the output.
Article
A synthetic hormone, 17α-ethynylestradiol (EE2), has received much attention due to its widespread occurrence and adverse effects on aquatic animals. The removal of EE2 from water has been very challenging mostly because of its low concentration in environmental waters. Here we report a simple and novel approach for concentrating EE2 through adsorp...
Article
Full-text available
A membrane-free micro-fluidic microbial fuel cell (μMFC) has been developed in this work, in which the bacteria-mediated organic fuel oxidation process is physically separated from the proton exchange process that occurs between the laminar co-flows of anolyte and catholyte streams on a micro-fluidic chip. This new strategy aims to shelter exoelect...
Article
Molecular mapping of live biofilms at submicron resolution presents a grand challenge. Here, we present the first chemical mapping results of biofilm extracellular polymeric substance (EPS) components in biofilms using correlative imaging be-tween super resolution florescence microscopy and liquid time-of-flight secondary ion mass spectrometry (ToF...
Article
In situ liquid secondary ion mass spectrometry (SIMS) enabled by system for analysis at the liquid vacuum interface (SALVI) has proven to be a promising new tool to provide molecular information at solid–liquid and liquid–vacuum interfaces. However, the initial data showed that useful signals in positive ion spectra are too weak to be meaningful in...
Article
Full-text available
In natural and engineered environments, microorganisms often co-exist and interact with various minerals or mineral-containing solids. Microorganism-mineral interactions contribute significantly to environmental processes, including biogeochemical cycles in natural ecosystems and biodeterioration of materials in engineered environments. In this min...
Article
We report a facile method for the anti-microbial modification of a thin-film composite polyamide reverse osmosis (RO) membrane. The membrane surface was first coated with polydopamine (PDA), whose reducing catechol groups subsequently immobilized silver ions in situ to form uniformly-dispersed silver nanoparticles (AgNPs) inside the coating layer....
Article
This article describes molecular design, synthesis and characterization of colloidal nanoparticles containing polycaprolactone-grafted conjugated polymers that exhibit strong far red/near infrared (FR/NIR) fluorescence for bioimaging. Specifically, we synthesized two kinds of conjugated polymer bottle brushes (PFTBout-g-PCL and PFTBin-g-PCL) with d...
Article
Using Pseudomonas syringae and Pantoea stewartii as model organisms, we quantified the influence of silver nanoparticles and single-wall carbon nanotubes on the production of different acylated homoserine lactones for quorum sensing (QS). This study reports for the first time on differential effects exhibited by nanomaterials on different QS signal...
Article
Membrane biofouling is a critical barrier for prolonged membrane operations. In the current study, silver nanocomposite osmotic membranes were fabricated to impart anti-microbial properties. The PAN–Ag nanocomposite substrates were prepared using one-step phase inversion. With additional crosslinked layer-by-layer (xLbL) assembly on the nanocomposi...
Article
Full-text available
The oleaginous yeast Rhodosporidium toruloides has great biotechnological potential. It accumulates a high amount of lipids which can be used for biofuels and also produces carotenoids which are valuable in the food and pharmaceutical industry. However, the location of these two hydrophobic products in the cell membrane prohibits its efficient harv...
Article
Full-text available
Introduction: As bioaerosol research attracts increasing attention, there is a need for additional efforts that focus on method development to deal with different environmental samples. Bioaerosol environmental samples typically have very low biomass concentrations in the air, which often leaves researchers with limited options in choosing the dow...
Data
Sampling Schedule and Standard Curves. Sampling schedules information and standard curves for bacteria, fungus and custom-GFP for qPCR Analysis. (XLSX)
Conference Paper
In most natural and engineered environments, microorganisms are commonly found closely associated with surfaces or interfaces in the form of multicellular aggregates known as biofilms. A number of studies have suggested that biofilm mode of life provides a range of advantages to the cells, especially under unfavourable conditions such as exposure t...