Bill Biligetu

University of Saskatchewan | U of S · Crop Development Centre

In late summer and fall, quality and quantity of forage are important for weight gain by grazing animals in western Canada. The objective of this study was to evaluate forage nutritive value, dry matter (DM) yield, and compatibility of crested wheatgrass [Agropyron cristatum (L.) Gaertn.], meadow bromegrass (Bromus riparius Rehm.), green needle gra...

Slender wheatgrass [Elymus trachycaulus (Link) Gould subsp. trachycaulus] is a native caespitose grass used for forage production and reclamation. The objective of this study was to quantify seed germination requirements of slender wheatgrass using thermal and hydro time models. Slender wheatgrass, San Luis, had a base temperature (T-b) of 9.48 deg...

Sainfoin (Onobrychis viciifolia Scop.) is a perennial forage legume that has received renewed interest in western Canada because of its desirable forage characteristics. The objective of this review is to summarize previous studies on the agronomy, forage yield, nutritive value, seed characteristics, and diseases and pests of sainfoin. In addition,...

Crested wheatgrass [Agropyron cristatum L. (Gaertn.)] is widely used for early spring grazing in western Canada and the development of late maturing cultivars which maintain forage quality for a longer period is desired. However, it is difficult to manipulate the timing of floral transition, as little is known about molecular mechanism of plant mat...

Molecular plant breeding with the aid of molecular markers has played an important role in modern plant breeding over the last two decades. Many marker-based predictions for quantitative traits have been made to enhance parental selection, but the trait prediction accuracy remains generally low, even with the aid of dense, genome-wide SNP markers....

Unmanned aerial vehicles (UAV)-based multi-spectral imaging could reduce the intensive labour required in phenotyping germplasm in crop breeding. The objectives of this study were to examine if UAV-based imaging could differentiate cicer milkvetch (Astragalus cicer L.) germplasm and identify UAV-based vegetation indices with correlations to its dry...

In Saskatchewan, red clover (Trifolium pratense L.) is often grown for seed production. However, there is no recognized or data supported seeding rate for seed production of red clover in Saskatchwan. The objectives of this study were to identify an optimal seeding rate for seed production and biological nitrogen fixation (BNF) and to examine their...

Alfalfa ( Medicago sativa L.) ‐ grass or alfalfa ‐ sainfoin ( Onobrychis viciifolia Scop.) mixtures are commonly used for pastures in western Canada because of their high forage quality and their low risk of causing frothy bloat in grazing animals. However, the proportion of these two legumes declines in mixed forage stands over time. The objective...

Background Genotype × environment interaction (GEI) slows genetic gains and complicates selection decisions in plant breeding programs. Forage breeding program seed sales often encompass large geographic regions to which the cultivars may not be adapted. An understanding of the extent of GEI in perennial, cool‐season forage grasses will facilitate...

Background Alfalfa (Medicago sativa L.) experiences many negative effects under salinity stress, which may be mediated by recurrent selection. Salt-tolerant alfalfa may display unique adaptations in association with rhizobium under salt stress. Results To elucidate inoculation effects on salt-tolerant alfalfa under salt stress, this study leverage...

Doubled haploidy (DH) methodology is used in many plant species to accelerate crop improvement and cultivar development; however not all species are amenable to the tissue culture technique. Experiments were undertaken to develop DH protocols for three perennial grasses [crested wheatgrass (Agropyron cristatum (L.) Gaertn.), hybrid bromegrass (Brom...

Fodder Galega (Galega orientalis Lam.) is a perennial legume adapted to the temperate regions of the world. The objective of this research was to compare the performance of fodder Galega to alfalfa (Medicago sativa L.), sainfoin (Onobrychis viciifolia Scop.), and cicer milkvetch (Astragalus cicer L.) in monocultures and grass–legume mixtures. From...

Doubled haploidy (DH) methodology is used in many plant species to accelerate crop improvement and cultivar development; however not all species are amenable to the tissue culture technique. Experiments were undertaken to develop DH protocols for three perennial grasses [crested wheatgrass (Agropyron cristatum (L.) Gaertn.), hybrid bromegrass (Brom...

Effects of individual single‐nucleotide polymorphism (SNP) markers and the size of “training” and “test” populations affect prediction accuracy in genomic selection (GS). This study evaluated 11 subsets of 4932 SNPs using six genetic additive methods to understand marker density in GS prediction in alfalfa (Medicago sativa L.). In the GS methods, t...

In Canada, plains bison (Bison bison bison) was assessed by The Committee on the Status of Endangered Wildlife in Canada as threatened. While bison are no longer at risk of demographic extinction, conservation programs remain challenged by the rarity of large populations and most bison are found in small, isolated, and confined herds. In this conte...

In Canada, new forage varieties need not undergo grazing trials before registration and sale. To evaluate the effect of grazing, six cool-season species including meadow bromegrass (Bromus riparius Rehmann), orchardgrass (Dactylis glomerata L.), sainfoin (Onobrychis viciifoila Scop.), and three alfalfa varieties (Medicago sativa L.) were establishe...

Warmer than normal late fall temperatures may change standard fall harvest management of alfalfa (Medicago sativa L.) in western Canada. The objective of this study was to evaluate the forage yield and nutritive value of three types of alfalfa cultivars under different harvest managements. A field experiment was conducted from 2013 to 2017 using rh...

Over the last decade, there has been a renewed interest in sainfoin (Onobrychis viciifolia Scop.), particularly among western Canadian forage and beef‐cattle producers. A major trigger has been the availability of new sainfoin cultivars with improved compatibility with alfalfa (Medicago sativa). These new cultivars in binary mixture with alfalfa si...

Intermediate wheatgrass (Thinopyrum intermedium; IWG) is a temperate perennial grass capable of performing in dual-purpose perennial grain cropping systems. It is valued for its ecosystem services and forage yield and quality that can be utilized in many livestock systems. Development for potential perennial grain yield has been the focus of breedi...

The effect of cicer milkvetch (Astragalus cicer L.) and sainfoin (Onobrychis viciifolia Scop.) on ruminal fermentation, methane production, and microbial nitrogen synthesis was assessed in two experiments. Experiment 1 analyzed two legumes, cicer milkvetch and sainfoin at two stages (vegetative and late flower) incubated with alfalfa (Medicago sati...

Cicer milkvetch (Astragalus cicer L.) is a non-bloating perennial forage legume suitable for stockpiled grazing in the fall because of its rapid regrowth and high nutritive value. Genetically diverse germplasm are needed for the development of improved cicer milkvetch cultivars that can provide consistent production across variable climatic conditi...

Background and Aims Salt-tolerant Halomonas maura and biological N fixing Rhizobium may increase salinity tolerance of alfalfa (Medicago sativa), especially for alfalfa populations with improved salt tolerance. The objectives of this study were 1) evaluate whether salt-tolerant bacteria and rhizobium increase salinity tolerance of alfalfa; 2) to as...

The objective of this study was to determine rumen degradation kinetics of new low-lignin alfalfa (Medicago sativa L.) cv. Hi-Gest®360 (HiGest) in comparison with conventional alfalfa cv. AC Grazeland (Grazeland) in monoculture and binary mixtures at different maturity stages. Two cultivars of alfalfa (HiGest, and AC Grazeland) and their binary mix...

Intercropping forage pea (Pisum sativum L.) with barley (Hordeum vulgare L.) or oat (Avena sativa L.) is an alternative way of cropping to improve forage yield and quality for hay production compared to monocropping. A 2‐year (2016–2017) field study was conducted at three sites in Saskatchewan, Canada, to evaluate forage production and biological n...

Genomic information on alfalfa adaptation to long-term grazing is useful for alfalfa genetic improvement. In this study, 14 alfalfa populations were collected from long-term grazing sites (> 25 years) across four soil zones in western Canada. Alfalfa cultivars released between 1926 and 1980 were used to compare degree of genetic variation of the 14...

A three-year (2018–2020) study was conducted in Lanigan (Thin Black soil zone) and Saskatoon (Dark Brown soil zone), SK Canada to compare low-lignin alfalfa (Medicago sativa) cv. Hi-Gest® 360 (HiGest) with alfalfa- cv. AC Grazeland (Grazeland) in monoculture and binary mixtures with hybrid bromegrass (HBG; cv. AC Success) for forage yield, nutrient...

Genomic information on alfalfa adaptation to long-term grazing is useful for alfalfa genetic improvement. In this study, 14 alfalfa populations were collected from long-term grazing sites (> 25 yrs) across four soil zones in western Canada. Six agro-morphological and three nutritive value traits were evaluated from 2018 to 2020. The genotyping-by-s...

The aim of this study was to evaluate physiological responses and elemental composition of three salt tolerant alfalfa (Medicago sativa L.) cultivars, 'Halo', 'Bridgeview', 'Rugged', and two intolerant cultivars 'Rangelander' and 'Vernal' under five salinity levels (0 dSm −1 , 4 dSm −1 , 8 dSm −1 , 12 dSm −1 and 16 dSm −1) in a sand based hydroponi...

The effects of perennial and annual forage systems on forage biomass and quality, grazing animal performance, and enteric methane emissions were evaluated over two summer grazing seasons in 2020 and 2021. Forage systems (treatments) included (1) AC Success hybrid bromegrass (Bromus riparius Rehm. × Bromus inermis Leyss.) + PS30006 alfalfa (Medicago...

The effect of synergies between saline-tolerant grass and legume species grown in saline soil, on yield, quality, composition, persistence, and weed suppression was studied in 2019–2021 in a moderately saline soil located in the dark brown soil zone near Saskatoon, Saskatchewan, Canada. ‘Halo’ alfalfa (ALF), a salt-tolerant cultivar of alfalfa, was...

Native forage legumes may have potential for summer/fall grazing in semiarid prairie regions in mixture with grasses. The objective of this study was to evaluate two native clovers in binary mixtures with the introduced grasses when harvested in July and September to simulate late summer or fall stockpile forage. Eight binary clover-grass mixtures...

A 5 yr experiment evaluated the effects of sod-seeding sainfoin and cicer milkvetch into monoculture grass (Lanigan, SK) or legume (Lethbridge, AB) stands on pasture productivity, steer performance, and economics. At Lanigan, sainfoin decreased (treatment × year P = 0.01) from 13% in year 1 to 2% in year 2 (% plant population) and did not differ th...

The potential for novel forage mixtures to address reduced herbage for late season grazing was investigated. Forage legumes, sainfoin (Onobrychis viciifolia Scop.) (SF) cvs. AC Mountainview, Shoshone, and Nova (MountainSF, ShoshoneSF, and NovaSF), cicer milkvetch (Astragalus cicer L.) cv. AC Veldt (CMV), Canadian milkvetch (Astragalus canadensis L....

The study was conducted at Saskatoon, SK, Canada in the Dark Brown soil zone (52°07′N,106°38′W), to compare low-lignin Hi-Gest®360 alfalfa (Hi-Gest; Medicago sativa L.) to a conventional alfalfa (AC Grazeland) under two cutting regimes. Each cultivar was seeded in 2017 as both a monoculture and binary mixture (binary; Hi-Gest+HB and Grazeland+HB) w...

Background Alfalfa ( Medicago sativa L.) production decreases under salt stress. Identification of genes associated with salt tolerance in alfalfa is essential for the development of molecular markers used for breeding and genetic improvement. Result An RNA-Seq technique was applied to identify the differentially expressed genes (DEGs) associated...

Soil salinity is a global concern and often the primary factor contributing to land degradation, limiting crop growth and production. Alfalfa (Medicago sativa L.) is a low input high value forage legume with a wide adaptation. Examining the tissue-specific responses to salt stress will be important to understanding physiological changes of alfalfa....

Information on the tiller development of hybrid bromegrass (Bromus inermis × Bromus riparius) is limited. The objective of this study was to evaluate the effect of clipping frequency at the vegetative and reproductive stages on the tiller development and concentrations of sugars of c.v ‘AC Knowles’ hybrid bromegrass compared with c.v ‘Kirk’ crested...

Condensed tannins in sainfoin (Onobrychis viciifolia) improve forage digestion and reduce the risk of ruminant bloat caused by grazing alfalfa (Medicago sativa). The objectives of this study were to evaluate the forage dry matter yield (DMY) and persistence of newer sainfoin cultivars in mixtures with alfalfa, and to determine the impact of harvest...

A 3-year study (2016, 2017, and 2018) was conducted to compare the effects of alfalfa (ALF; cv. Yellowhead) (Medicago falcata L.) or sainfoin (SF; cv. AAC Mountainview) (Onobrychis viciifolia Scop.) in mixtures with either Russian wildrye (RWR; cv. Tom) (Psathyrostachys junceus [Fisch.]) or hybrid bromegrass (HBG; cv. Success) (Bromus riparius Rehm...

Many questions remain concerning the viability and productivity of seeding native legumes in the Canadian Prairies for forage production. Field research was conducted with four native legume species (Astragalus flexuosus, Dalea purpurea, Hedysarum boreale and Vicia americana) to evaluate performance in Swift Current and Saskatoon, SK. The experimen...

Objective of this study was to evaluate forage yield, quality, botanical composition, persistence, and weed density of salt tolerant forage mixtures in saline soil. An area representing a gradient of soil salinity near the Livestock and Forage Centre of Excellence in Clavet, SK was seeded on June 27, 2019 using the 4 forage mixtures: a saline toler...

Cicer milkvetch (Astragalus cicer L.) (CMV) is a non-bloating, perennial legume that has shown persistence under grazing. Limited information is available on its seedling establishment and subsequent forage yield and nutritive value in alfalfa (Medicago sativa L.) mixtures. Field plots were seeded in May 2013 at Melfort, SK, Canada to evaluate ‘Oxl...

Alfalfa (Medicago sativa L.) is one of the most important forage crops in the world. The objectives of this study were to assess alfalfa yield improvement in the Canadian prairies, and determine critical climatic factors influencing alfalfa yield in different soil zones. Forage yield data of alfalfa cultivars tested from 1997 to 2011 in the Western...

Single-cut red clover (Trifolium pratense L.) is an important forage seed crop in Western Canada due to its winter hardiness and economical value. However, infestation of lesser clover leaf weevil (Hypera nigrirostris F.) can lead to drastic yield losses (over 50%). The aim of this research was to evaluate the effectiveness of insecticides deltamet...

In Canada, new forage varieties need not undergo grazing trials before registration and sale. As such, little is known about forage performance under grazing, or how animal preference and temperament affect grazing behaviour. To determine these effects, 6 cool-season forage species including meadow bromegrass (Bromus bieberseinii), orchardgrass (Da...

Crested wheatgrass [Agropyron cristatum (L.) Gaertn.] provides high quality, highly palatable forage for early season grazing. Genetic improvement of crested wheatgrass has been challenged by its complex genome, outcrossing nature, long breeding cycle, and lack of informative molecular markers. Genomic selection (GS) has potential for improving tra...

The objective of this study was to evaluate the inclusion rate of pea hay in barley or oat hay diets for beef cattle. Six ruminally cannulated heifers (407 ± 38 kg) were used in a 6 × 6 Latin square (25 d periods) with a 2 × 3 factorial design. Treatments included whole-crop barley or oat hay with pea hay blended in to achieve inclusion rates of 0%...

Alfalfa (Medicago sativa L.) is an important legume forage crop. However, its genetic improvement for salt tolerance is challenging, as alfalfa’s response to salt stress is genetically and physiologically complex. A review was made to update the knowledge of morphological, physiological, biochemical, and genetic responses of alfalfa plants to salt...

The objective of this study was to compare low-lignin Hi-Gest® 360 alfalfa to a conventional legume in both monoculture and binary mixtures. Two varieties of alfalfa (monoculture) [Medicago sativa L. cv. AC Grazeland (Grazeland) and Hi-Gest 360 (Hi-Gest] or in mixture (binary) with AC Success hybrid bromegrass (HB) (Grazeland+HB and Hi-Gest+HB) wer...

Breeding forage crops for high yields of digestible biomass along with improved resource-use efficiency and wide adaptation is essential to meet future challenges in forage production imposed by growing demand, declining resources, and changing climate. Bromegrasses (Bromus spp.) are economically important forage species in the temperate regions of...

The objective of this study was to evaluate the stage of maturity at harvest for pea hay (Pisum sativum L., c.v. CDC Horizon) on dry matter intake (DMI), eating behavior, ruminal fermentation, and digestibility when fed to beef heifers. Pea hay was cut at EARLY (defined to occur when flat pods were on one or more nodes), MID (when seeds filled the...

The objective was to evaluate the effect of inclusion rate of pea hay in barley and oat hay when fed to beef cattle. Six ruminally-cannulated heifers (407 ± 38 kg) were used in a 6 × 6 Latin square with a 2 × 3 factorial design and 25-d periods. Treatments included barley or oat hay blended with pea hay to achieve inclusion rates of 0, 15, or 30% (...

The objective of this experiment was to evaluate stage of maturity at harvest for pea hay on DMI, eating behavior, ruminal fermentation, and digestibility when fed to beef heifers. Pea hay was harvested at early, mid, and late stages of maturity. Early was defined as observing flat pods at one or more nodes. Mid stage was defined as seeds filling t...

The accumulation and distribution of salt ions in leaf, stem and root tissues of salt-stressed alfalfa were studied using X-ray fluorescence spectroscopic at Canadian Light Source, SK.

Limited information is available on cicer milkvetch (CMV, Astragalus cicer L.) performance in mixtures with grasses. A field trial was sown at Melfort, SK, Canada, in May 2013 to evaluate forage accumulation, nutritive value, and botanical composition of different mixtures of grasses and CMV under two‐cut and three‐cut harvest management from 2014...

Chloroplast (cp) genomics will play an important role in the characterization of crop wild relative germplasm conserved in worldwide gene banks, thanks to the advances in genome sequencing. We applied a multiplexed shotgun sequencing procedure to sequence the cp genomes of 25 Avena species with variable ploidy levels. Bioinformatics analysis of the...

The objectives of this study were to characterize crested wheatgrass (Agropyron cristatum L.) germplasm for plant maturity and associated agronomic characteristics to identify germplasm with late maturity. A field trial was established in July 2014 at Saskatoon, Canada using 45 crested wheatgrass accessions representing materials from 18 countries...

The objective of this study was to understand the effects of salt stress on alfalfa from the whole plant to sub-cellular levels. Five alfalfa cultivars were grown in five gradients of salt stress of 0, 4, 8, 12 and 16 dS m-1 electrical conductivity (EC) in a sand-based hydroponic system for 12 weeks in the greenhouse. The elemental concentrations i...

The accumulation and distribution of organic compounds in leaf, stem and root tissue of salt-stressed alfalfa were studied using FITR spectroscopy at Canadian Light Source, SK.

Annual companion crops are often used in new seedings of perennial forages in western Canada. Fast-establishing grass species that do not overwinter in this region have potential as companion crops to increase seeding year yields without reducing perennial forage establishment. Trials were seeded in two different years at Saskatoon and Melfort, SK,...

Sainfoin (Onobrychis viciifolia) is a forage legume of renewed interest in western Canada, with equally weighted advantages and disadvantages. In agronomic attributes, sainfoin is, at best, equal in yield, crude protein content and persistence to alfalfa (Medicago sativa). Sainfoin is equally or more palatable than alfalfa to ruminants, with compar...

Sainfoin (Onobrychis viciifolia) is a forage legume of renewed interest in western Canada, with equally weighted advantages and disadvantages. In agronomic attributes, sainfoin is, at best, equal in yield, crude protein content and persistence to alfalfa (Medicago sativa). Sainfoin is equally or more palatable than alfalfa to ruminants, with compar...

The effects of AC Yellowhead alfalfa (ALF), sainfoin cultivars; AC Mountainview, Nova, Shoshone, Delaney, AC Veldt cicer milkvetch (VELDT), Great Plains Canadian milkvetch (GRT), AC Lamour purple prairie clover and Antelope white prairie clover in binary mixtures with either Tom Russian wildrye (RWR), AC Success hybrid bromegrass (HBG) or Admiral m...

The inclusion of forage pea (Pisum sativum L.) in mixtures with forage barley (Hordeum vulgare L.) or oat (Avena sativa L.) for greenfeed or silage production is gaining interest in western Canada. A twelve-different combination of monocultures and mixtures were evaluated for forage dry matter (DM) yield and forage quality in three soil zones of Sa...

This study used an in-vitro batch culture assessment to examine DMD, ruminal fermentation and CH4 production associated with AAC Mountainview, a new variety of sainfoin (cv. Onobrychis viciifolia Scop.) alone or in combination with hybrid bromegrass (cv. AC Knowles) at 3 seeding ratios (100, 70:30 and 50:50) and 2 harvest dates in mid June and mid...

This study assessed the relative potential of 7 annual species for stockpiled forage production to extend the grazing season for beef cows. The species and cultivars were: corn (Zea mays L.) cv. Fusion; foxtail millet (Setaria italica (L.) P. Beauvois) type Golden German; oat (Avena sativa L.) cv. Haymaker; fall rye (Secale cereale L.) cv. Hazlet;...

This study assessed supplementing AAC Mountainview sainfoin, with 2 sources of canola oil (CO; commodity and high oleic acid variety) at 3 inclusion rates (0, 3, 5% DM), on in-vitro fermentation, DMD and CH4 production. Sainfoin was seeded alone or in combination with hybrid bromegrass at 3 seeding ratios (100, 70:30 and 50:50). An alfalfa-meadow b...

A 2-yr study was conducted using a randomized complete block design to determine the effects of grazing AC Yellowhead alfalfa (ALF) and AC Mountainview sainfoin (SF) in binary mixtures with either Tom Russian wildrye or AC Success hybrid bromegrass (HBG) in late summer at Lanigan and Swift Current, SK. Forty-eight Angus steers (404 + 18 kg in yr 1;...

This study assessed supplementing AAC Mountainview sainfoin, with 2 sources of canola meal (CM; commodity and high oleic acid variety) at 3 inclusion rates (0, 10, 20% DM), on in-vitro fermentation, DMD and CH4 production. Sainfoin was seeded alone or in combination with hybrid bromegrass at 3 ratios (100, 70:30 and 50:50). An alfalfa-meadow bromeg...