Biao Wu

Biao Wu
Chinese Academy of Fishery Sciences | CAFS · Yellow Sea Fisheries Research Institute

PhD

About

89
Publications
8,155
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859
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September 2010 - June 2014
Ocean University of China
Position
  • PhD Student

Publications

Publications (89)
Article
Full-text available
Background/Objectives: The Pacific abalone Haliotis discus hannai originated in cold waters and is an economically important aquaculture shellfish in China. Our goal was to clarify the current status of the genetic structure of Pacific abalone in China. Methods: In this study, eighteen polymorphic EST-SSR loci were successfully developed based on t...
Article
Full-text available
The razor clam Sinonovacula constricta, a significant marine bivalve species, inhabits estuaries and encounters salinity stress. Despite its commercial importance, there is limited understanding of its adaptive mechanisms to high salinity. Aldehyde dehydrogenases (ALDHs), which belong to the NAD(P)+-dependent superfamily, play a crucial role in str...
Preprint
Full-text available
The Pacific abalone Haliotis discus hannai originated in cold waters, but as an economically important aquaculture shellfish in China, there has been a large-scale transfer of fish to Fujian Province in southern China for culture. Our goal was to clarify the current status of the genetic structure of Pacific abalone in China. In this study, eightee...
Article
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Single-nucleotide polymorphisms (SNPs) are the most commonly used DNA markers in population genetic studies. We used the Illumina HiSeq4000 platform to develop single-nucleotide polymorphism (SNP) markers for Manila clam Ruditapes philippinarum using restriction site-associated DNA sequencing (RAD-seq) genotyping. Eighty-eight SNP markers were succ...
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Background Ovarian development is an important prerequisite and basis for animal reproduction. In many vertebrates, it is regulated by multiple genes and influenced by sex steroid hormones and environmental factors. However, relative information is limited in shellfish. To explore the biological functions and molecular mechanisms of mRNA and non-co...
Article
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China has the largest production yield of Manila clam Ruditapes philippinarum in the world. Most of the clam seeds for aquaculture are mainly derived from artificial breeding in southern China, likely resulting in the loss of genetic variation and inbreeding depression. To understand the genetic and haplotype diversity of R. philippinarum, 14 clam...
Article
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The Pacific abalone is an economically important cold-water shellfish. With its widespread culture, high temperature has become a key abiotic factor for the high mortality of Pacific abalone in summer, particularly in the south of China. To understand the molecular regulatory mechanisms of thermal stress response in Pacific abalone for further anal...
Article
Quantitative real-time PCR (qRT-PCR) has been widely used for gene expression analysis, and selection of reference genes is a key point to obtain accurate results. To find out optimal reference genes for qRT-PCR in Manila clam Ruditapes philippinarum in response to hypoxia, different tissues were used and compared to evaluate the stability of candi...
Article
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Temperature is an important environmental factor affecting metabolism, growth and life activities of marine bivalves. To reveal the molecular and metabolic responses to heat stress, comparative transcriptomics and metabolomics were performed in the commercially important bivalve, Manila clam Ruditapes philippinarum. Comparative transcriptomics reve...
Article
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The Manila clam (Ruditapes philippinarum) is one of the most commercially important bivalves along the coast of China. With the continuous expansion of clam farming scale, it may lead to some serious problems, including loss of genetic variation, inbreeding depression, and reduced effective population size (Ne). In the present study, eleven microsa...
Article
Marking technology is essential to evaluate the growth performance of shellfish species in aquaculture. Some fluorochromes have been widely used in shellfish growth studies, but their marking quality remains unresolved. In order to establish an effective marking technology in bivalves, three fluorochromes are used to evaluate the suitability of in...
Article
Hypoxia has become one of the major environmental problems in the aquaculture industry. As one of the most commercially important bivalves, Manila clam Ruditapes philippinarum may be suffering substantial mortality attributable to hypoxia. The physiological and molecular responses to hypoxia stress in Manila clam were evaluated at two levels of low...
Article
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Glycogen, a stored form of glucose, is an important form of energy for aquatic shellfish, contributing to the flavor and quality of the oyster. The glycogen synthase (GYS) and glycogen synthase kinase 3β (GSK3β) are two major enzymes in the glycogenesis. However, the information of the two genes in the Jinjiang oyster Crassostrea ariakensis remains...
Article
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The Chinese razor clam (Sinonovacula constricta) is an important for Chinese aquaculture marine bivalve that naturally occurs across intertidal and estuarine areas subjected to significant changes in salinity level. However, the information on the molecular mechanisms related to high salinity stress in the species remain limited. In this study, nin...
Article
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Glycogen serves as the principal energy reserve for metabolic processes in aquatic shellfish and substantially contributes to the flavor and quality of oysters. The Jinjiang oyster ( Crassostrea ariakensis) is an economically and ecologically important species in China. In the present study, RNA sequencing (RNA-seq) and assay for transposase-access...
Article
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Hypoxia tolerance and adaptive regulation are important for aquatic animals, especially for species with poor mobility, such as most bivalves. Previous studies have confirmed that the blood clam Scapharca broughtonii has strong hypoxia resistance. However, the molecular mechanism supporting its hypoxic tolerance is still largely limited. To further...
Article
Salinity is an important environmental factor affecting the growth and survival of marine organisms. Marine shellfish possess the efficient osmoregulatory mechanism to cope with either hypo- or hyperosmotic stress. However, little is known about the molecular and metabolic adaptation involved in the osmotic regulation of these intertidal habitants....
Article
Myosin found in scallop adductor muscles is a regulatory protein that is responsible for muscle contraction. Myosin heavy chains (MyHCs) form the core component of myosin and play important roles in myofibril assembly and maintenance. In the present study, we obtained the full-length cDNA sequences encoding MyHC from the Yesso scallop Patinopecten...
Article
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Scallops are economically important shellfish widely cultured in China. In this study, the agglomeration characteristics of the scallop aquaculture industry in Chinese coastal provinces were explored based on data from the China Fishery Statistical Yearbook from 2012 to 2020 and the comparative advantage and concentration level of the scallop breed...
Article
Increasing evidences point to the potential role of microRNAs (miRNAs) in muscle growth and development in animals. However, knowledge on the identity of miRNAs and their targets in molluscs remains largely unknown. Scallops have one large adductor muscle, composed of fast (striated) and slow (smooth) muscle types, which display great differences i...
Article
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Paramyosin is an important myofibrillar protein in molluscan smooth muscle. The full-length cDNA encoding paramyosin has been identified from Yesso scallop Patinopecten yessoensis. The length of paramyosin molecule has been found to be 3715 bp, which contains an open reading frame (ORF) of 2805 bp for 934 amino acid residues. Characterization of P....
Article
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Salinity can affect the physiological and productive characteristics of bivalves. The razor clam Sinonovacula constricta naturally distributing in intertidal zones and estuarine waters is extensively cultured in China. High-salinity is a serious threat to its culture, especially in pond culture model. Here, we investigated the effects of high-salin...
Article
Knowledge of early development in bivalves is of great importance to understand the function of animal organ systems and the evolution of phenotypic diversity. Manila clam (Ruditapes philippinarum) is an economically important bivalve living in marine intertidal zones. To determine the pattern of muscle development in the clams, we investigate the...
Article
The Jinjiang oyster Crassostrea ariakensis, naturally distributing in estuarine regions with low salinity, is an important economic and ecological species in China. However, studies on its genomics and population genetics remain lacking. Here, we assembled the chromosome‐level genome of a female C. ariakensis and re‐sequenced 261 individuals from f...
Article
Many proteins are known to be phosphorylated, affecting important regulatory factors of muscle quality in the aquatic animals. The striated and smooth adductor muscles of Yesso scallop Patinopecten yessoensis were used to investigate muscle texture and identify phosphoproteins by histological methods and phosphoproteomic analysis. Our present study...
Article
Studies on cell atlas in marine invertebrates provide a better understanding of cell types, stem cell maintenance, and lineages of cell differentiation. To investigate the molecular features of various cell types in molluscan muscles, we performed single-cell RNA sequencing (scRNA-seq) to map cell types in scallop adductor muscles. We uncovered the...
Article
Background The ark shell (Scapharca broughtonii) is one of the most economically important mollusks in the Bohai Sea and Yellow Sea of China. In recent years, ark shells from the Korean population were introduced to China for seed propagation and culture.Objective To explore the impact of the introduction of Korean ark shell on the genetic diversit...
Article
Mudflat shellfish have evolved well-adapted strategies for coping with dynamic environmental fluxes and stressful conditions, including oxygen availability. The Manila clams Ruditapes philippinarum are worldwide cultured shellfish in marine intertidal zone, which usually encounter great risk of acute hypoxia exposure in coastal habitats. To reveal...
Article
The pen shell, Atrina pectinata, distributes globally. It is one of the most important edible bivalves in east Asian countries. However, there are multiple difficulties in rearing pen shell larvae and juveniles because of their high mortality. To understand the mechanism underlining such high mortality at the early embryonic development stage, we o...
Article
Myostatin (MSTN) belongs to the transforming growth factor β (TGF-β) family, which plays a critical role in negatively regulating muscle growth in vertebrates. However, little is known about its function in muscle growth of scallops that possess a large adductor muscle composed of striated and smooth muscle fibers. In this study, we first indentify...
Article
Though the larval development of bivalves has been extensively studied for commercial purposes, the dynamic development of larval muscle system remains largely unknown. In this study, we characterized the larval muscle system at different developmental stages (D-shaped veligers, umbo veligers and spats) in the bay scallop (Argopecten irradians) by...
Article
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Salinity is an important environmental factor of osmotic pressure which is closely related to growth and development of aquatic organisms. Some physiological studies regarding to challenge of salinity have been reported in marine shellfish. However, information about the effects of acute low-salinity stress on the enzyme activities in ark shell, Sc...
Preprint
Full-text available
Background:The Yesso scallop, Patinopecten (Mizuhopecten) yessoensis, is a commercially important bivalve in the coastal countries of Northeast Asia. It has complex modes of sex differentiation, but knowledge of the mechanisms underlying this sex determination and differentiation is limited. Results:In this study, the gonad tissues from females and...
Article
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An 8‐week feeding trial was conducted to assess the interaction between dietary protein levels and fish growth, digestibility and activity of immunity‐related enzymes of Plectropomus leopardus. Five diets with different protein levels (400 g/kg, 450 g/kg, 500 g/kg, 550 g/kg and 600 g/kg protein) were designed. P. leopardus fed with 500 g/kg, 550 g/...
Article
Full-text available
Background: The Yesso scallop, Patinopecten (Mizuhopecten) yessoensis, is a commercially important bivalve in the coastal countries of Northeast Asia. It has complex modes of sex differentiation, but knowledge of the mechanisms underlying this sex determination and differentiation is limited. Results: In this study, the gonad tissues from female...
Preprint
Full-text available
Background:The Yesso scallop, Patinopecten (Mizuhopecten) yessoensis, is a commercially important bivalve in the coastal countries of Northeast Asia. It has complex modes of sex differentiation, but knowledge of the mechanisms underlying this sex determination and differentiation is limited. Results:In this study, the gonad tissues from females and...
Preprint
Full-text available
Background:The Yesso scallop, Patinopecten (Mizuhopecten) yessoensis, is a commercially important bivalve in the coastal countries of Northeast Asia. It has complex modes of sex differentiation, but knowledge of the mechanisms underlying this sex determination and differentiation is limited. Results:In this study, the gonad tissues from females and...
Article
Full-text available
Background The blood clam, Scapharca (Anadara) broughtonii, is an economically and ecologically important marine bivalve of the family Arcidae. Efforts to study their population genetics, breeding, cultivation, and stock enrichment have been somewhat hindered by the lack of a reference genome. Herein, we report the complete genome sequence of S. br...
Preprint
Full-text available
Background:The Yesso scallop, Patinopecten (Mizuhopecten) yessoensis, is a commercially important bivalve in the coastal countries of Northeast Asia. It has complex modes of sex differentiation, but knowledge of the mechanisms underlying this sex determination and differentiation is limited. Results:In this study, the gonad tissues from females and...
Preprint
Full-text available
Background: The blood clam, Scapharca (Anadara) broughtonii, is an economically and ecologically important marine bivalve of the Family Arcidae. The efforts that have been made to study their population genetics, breeding, cultivation and stock enrichment were somewhat hindered by the lack of a reference genome. Here, we reported the complete genom...
Article
Full-text available
As one crucial member of heat shock proteins (HSPs) family, HSP70s play many important roles in a large amount of physiological processes including immune response. However, information regarding HSP70 in ark shell Scapharca broughtonii is still rather limited. Here the full-length cDNA of HSP70 gene (named SbHSP70) of S. broughtonii was identified...
Article
The development of bivalves has been extensively studied over the last 150 years. Despite this, the developmental dynamics of myogenesis in bivalves remains largely unknown, particularly at the early developmental stages. In the present study, we investigate the characteristics of muscle development of Yesso scallop Patinopecten yessoensis by phall...
Article
Scapharca broughtonii is one of the most important Arcidae aquaculture species in the Asia-Pacific region. We aimed to investigate the immune responses of hemocytes from ark shell S. broughtonii hemolymph against pathogens. Hemocyte ultrastructure and immunological activity in response to Vibrio anguillarum challenge were observed by scanning and t...
Article
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Background: Scallops possess striated and catch adductor muscles, which have different structure and contractile properties. The striated muscle contracts very quickly for swimming, whereas the smooth catch muscle can keep the shells closed for long periods with little expenditure of energy. In this study, we performed proteomic and transcriptomic...
Article
Hemoglobin, the main component of haemolymph, is widely distributed in animals. Although its important oxygen transport functions has been extensively reported, studies on the immune function of hemoglobin in mollusc are few. Research on immune of hemoglobin of ark shell Scapharca broughtonii attracted more and more attention due to its ownership o...
Article
Blepharipoda liberate Shen is a commercially valuable seafood species that has important ecological significance in Shandong Province, China. Although B. liberate is crustacean, its external characteristics are not entirely those of shrimps or crabs. The question of whether B. liberate is a shrimp or a crab has been debated in recent years. We stud...
Article
DNA 条形码旨在通过PCR 技术获得一段DNA 序列, 在物种水平上对现存生物类群和未知生物材料进行识 别和鉴定。线粒体细胞色素C 氧化酶I (COI)基因是常用DNA 条形码基因之一, 为研究COI 基因作为DNA 条形 码在贝类系统进化中的评估效果, 本文利用PCR 技术扩增获得了60 个贝类物种的353 条COI 基因序列, 通过聚类 法构建了neighbor-joining (NJ)进化树, 同时还对7 个物种不同地理群体的遗传进化情况进行了分析。结果表明, 选 用的COI 基因引物在大多数贝类中通用性较强, 除在珍珠贝目中的扩增效率只有10%以外, 在整个研究中扩增效 率达到82.7%;60 个物种中除太平洋潜泥蛤(Panopea abrupta)、沼蛤(Limnoperna f...
Article
DNA barcoding has been widely used in the fields of taxonomy, identification, phylogenetic evolution, and population genetic analysis of marine shellfish. To further evaluate the identification validity of different DNA barcoding genes in marine shellfish, sequences of COI, 16S rRNA, 18S rRNA, and 28S rRNA from Veneroida were randomly downloaded fr...
Article
As a molecular chaperone, HSP90 plays pivotal roles in protein process in a normal condition. However, it could be involved in the immune response to pathogens invasion in other abnormal conditions. In the present study, we cloned the full length cDNA of heat shock protein 90 from ark shell Scapharca broughtonii (designated SbHSP90) by rapid amplif...
Article
The pen shell (Atrina pectinata) is a large wedge-shaped bivalve, which belongs to family Pinnidae. Due to its large and nutritious adductor muscle, it is the popular seafood with high commercial value in Asia-Pacific countries. However, limiting genomic and transcriptomic data have hampered its genetic investigations. In this study, the transcript...
Article
Melanin is one of the essential compounds in the pigments of molluscan shells. However, the effects of melanin on color variations in molluscs are largely unknown. Our previous study suggests that Yesso scallop Patinopecten yessoensis might contain melanin pigment in the dark brown shell. We therefore isolated melanin from the pigmented shells usin...
Article
It has been reported in various species that maternal immune factors, such as antibodies, lysozyme, and lectins, could be transferred to offspring from mother via egg and milk, and play important immune roles in developmental stage of embryos and larvae before immune system maturity. However, the expression characteristics of immune factors in diff...
Article
The ark shell Scapharca broughtonii is a commercially important shellfish in China. Alserver's solution (AS), modified Alserver's solution (MAS) and Heparin sodium solution (HSS) are common anticoagulants used for shellfish blood. To observe the immune response mediated by its hemocytes, we challenged in vivo S. broughtonii hemolymph with Vibrio an...
Article
Marine animals exhibit a variety of biological rhythms, such as solar and lunar-related cycles; however, our current molecular understanding of biological rhythms in marine animals is quite limited. Identifying and understanding the expression patterns of clock genes from available transcriptomes will help elucidate biological rhythms in marine spe...
Article
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The Yesso scallop Patinopecten yessoensis displays polymorphism in shell colors, which is of great interest for the scallop industry. To identify genes involved in the shell coloration, in the present study, we investigate the transcriptome differences by Illumina digital gene expression (DGE) analysis in two extreme color phenotypes, Red and White...
Article
Catalase (EC 1.11.1.6) is an important antioxidant enzyme that protects aerobic organisms against oxidative damage by degrading hydrogen peroxide to water and oxygen. In the present study, the complete cDNA of Catalase in ark shell Scapharca broughtonii (named SbCAT) was cloned by RT-PCR and rapid amplification of cDNA ends technique, which contain...
Article
Yesso scallop Patinopecten yessoensis is an economically important marine bivalve species in aquaculture and fishery in Asian countries. The colors of the left and right shells are obviously distinct, typically having reddish-brown for the left and white for the right. This left-right asymmetric pigment pattern is a very unique phenomenon among inv...
Article
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The growth superiority of hybrid scallop Chlamys farreri (♀) × Patinopecten yessoensis (♂), as the following successive generation selection have been reported. However, the data about the genetic diversity in those population remains unexplored. In this study, the genetic structure analysis of F1, F2 and F3 were conducted by PCR with 10 Simple Seq...
Article
Ferritin, the principle cytosolic iron storage protein in the majority of living organisms, has important roles during immune process in invertebrates. Detailed information about ferritin in the ark shell Scapharca broughtonii, however, has been very limited. In this study, full-length ferritin (termed SbFer) was cloned by the rapid amplication of...
Article
Full-text available
The Yesso scallop Patinopecten yessoensis is an economically important marine bivalve species in aquaculture and fishery in Asian countries. However, limited genomic resources are available for this scallop, which hampers investigations into molecular mechanisms underlying their unique biological characteristics, such as shell formation and pigment...