Bhupal Govinda Shrestha

• PhD
• Professor at Kathmandu University

Background Cancer is one of the major health problems worldwide and medicinal plants constitute a common alternative for cancer treatment having no or fewer side effects. This study aimed to assess total phenolic (TPC) and flavonoid (TFC) contents, antioxidant, biological activities (especially antibacterial, antifungal, and anticancer), and chemic...

Background Cancer is one of the major health problems worldwide and medicinal plants constitute a common alternative for cancer treatment having no or less side effects. This study was aimed to assess total phenolic (TPC) and flavonoid (TFC) contents, antioxidant, biological activities (especially antibacterial, antifungal and anticancer) and chemi...

Background The development of digital technologies and the evolution of open innovation approaches have enabled the creation of diverse virtual organizations and enterprises coordinating their activities primarily online. The open innovation platform titled “International Natural Product Sciences Taskforce” (INPST) was established in 2018, to bring...

Background: The development of digital technologies and the evolution of open innovation approaches have enabled the creation of diverse virtual organizations and enterprises coordinating their activities primarily online. The open innovation platform titled "International Natural Product Sciences Taskforce" (INPST) was established in 2018, to brin...

Background: The development of digital technologies and the evolution of open innovation approaches have enabled the creation of diverse virtual organizations and enterprises coordinating their activities primarily online. The open innovation platform titled “International Natural Product Sciences Taskforce” (INPST) was established in 2018, to brin...

The development of digital technologies and the evolution of open innovation approaches have enabled the creation of diverse virtual organizations and enterprises coordinating their activities primarily online. The open innovation platform titled “International Natural Product Sciences Taskforce” (INPST) was established in 2018, to bring together i...

Breast cancer is the most common cancer among women around the world. Human Epidermal growth factor Receptor-2 (HER2) is a membrane tyrosine kinase overexpressed in 30% of human breast cancers; thus, it serves as an important drug target. Currently available HER2 inhibitor lapatinib targets the ATP binding site of the cytoplasmic kinase domain, blo...

The present work evaluated the inactivation efficiency of atmospheric pressure cold plasma (APCP) against three pathogenic microorganisms. We have successfully developed a plasma device that can generate atmospheric pressure cold argon plasma of low temperature (24 0-27 0 C) downstream using a high-voltage power source (6 kV) operating at a frequen...

Breast cancer is the most common cancer among women around the world. Human Epidermal growth factor Receptor-2 (HER2) is a membrane tyrosine kinase overexpressed in 30% of human breast cancers; thus, it serves as an important drug target. Currently available HER2 inhibitor lapatinib targets the ATP binding site of the cytoplasmic kinase domain, blo...

This research discusses the design and synthesis of therapeutic peptides conjugated to dichloroacetyl chloride (DAC) followed by their characterization and cytotoxic evaluation. The conjugates demonstrated cytotoxic effect following in vitro evaluation using MTT and the brine shrimp lethality assay. Significant cell death for octaarginine-DAC conju...

In this paper, we demonstrated the application of atmospheric pressure argon plasma jet (APAPJ) in biomedical science such as cancer therapy and biomedical engineering such as surface modification of polystyrene Petri dishes for animal cell culture. We observed that APAPJ, when exposed to breast cancer cell line (MDA-MB-231) for 60 seconds at a dis...

Ethnomedicinal plants are being used as a source of medicine from ancient time but they lack the proof of modern scientific evidence for their effectiveness. This study focuses on the evaluation of phytochemical, antimicrobial, antioxidant properties of one of the ethnomedicinal plant Agave americana from Dhulikhel region of Nepal. The plant extrac...

Nepal harbors distinct potential for traditional medicinal plants with its rich climatic and geographical variance, biodiversity and endogenous knowledge. Traditional medicinal plants are being used from ancient time in Nepal. Many communities and tribes of Nepal carries the knowledge of using medicinal plants for curing various diseases for their...

p> Background: Chemotherapy agents alter serum lipids and C-peptide in cancer patients increased incidence of cardiovascular disorders and diabetes, although mechanisms for these are not known. Diabetes and cardiovascular riskis the highly concerned chemotherapy induced complications among cancer patients. This study is aimed to evaluate the serum...

Breast cancer (BC) is the most common type of cancer worldwide, being a major cause of morbidity in female. In Nepal it is the second most common type of cancer among females of perimenopausal age group. More than one quarter of the BC diagnosed in young Nepalese female, with familial history of breast cancer, early pregnancy, longer lactation and...

Background There is growing interest in the use of plants for the treatment and prevention of cancer. Medicinal plants are currently being evaluated as source of promising anticancer agents. In this paper, we have investigated the anticancer potential of plant Allium wallichii, a plant native to Nepal and growing at elevations of 2300–4800 m. This...

There is growing interest in the use plants for the treatment and prevention of cancer and are currently being evaluated as promising anticancer agents. In this paper, we investigated the anti-microbial, anti-oxidant, phytotoxic and immunomodulatory of plant Bauhinia variegata and also established the presence of important phytoconstituents which m...

In today’s world, search for natural medicines is increasing as a result of drug resistance of pathogens and also due to negative consequences of antibiotic. Presence of phytochemicals, antioxidant potential and antimicrobial activity of Artocarpus heterophyllus was carried out in this study. Leaf of this plant was subjected to warm extraction with...

Novel magnetic composite nanoparticles (MCPs) were successfully synthesized by ex situ conjugation of synthesized ZnO nanoparticles (ZnO NPs) and Fe3O4 NPs using trisodium citrate as linker with an aim to retain key properties of both NPs viz. inherent selectivity towards cancerous cell and superparamagnetic nature, respectively, on a single system...

Fossil fuel as a source of energy to run motor vehicles has been used widely because they easily generate energy; however, they have direct impact on the environment. Their combustion leads to a great deal of air pollution and contributes to global warming and is the cause of acid rain. Furthermore, being a nonrenewable source of energy, the fear o...

Cellulolytic/Xylanolytic microorganisms such as bacteria and fungi are accountable for conversion of lignocellulosic biomass in soil. Despite this vast number of cellulose/xylanase producers, there is a deficiency of microorganisms that can produce a significant amount of cellulase/xylanase enzyme to proficiently degrade cellulose/xylan to fermenta...

Background: Nanoparticles (NPs) are receiving increasing interest in biomedical research owing to their comparable size with biomolecules, novel properties and easy surface engineering for targeted therapy, drug delivery and selective treatment making them a better substituent against traditional therapeutic agents. ZnO NPs, despite other applicat...

Research on medicinal plants are important to Nepal because most of its rural population relies on it as mode of medicine. Medicinal plants namely Swertia chirayita and Dendrobium amoenum were collected from mid hills of Nepal. The present study was undertaken to find the antimicrobial activity, phytochemical presence and their cytotoxicity in diff...

Traditional medicine involves the use of different plant extracts or their bioactive constituents. Medicinal plants are an important source of phytochemicals that offer traditional medicinal treatment of various ailments. This type of study provides the health application at affordable cost. The objective of this research was to test for the presen...

— Zinc is the most abundant trace element which has role in genetic stability and function, present in the cell nucleus, nucleolus and chromosomes, and stabilizes the structure of DNA, RNA and ribosomes and found in many Zinc binding proteins. The purpose of this study is to access the Zinc cytotoxicity, total cellular Zinc content and total Zinqui...

Over 70% of anticancer drug has their origin as natural products and only a small part of the massive reservoir of the bioactive components on Earth has been studied. This study comprises the phytochemical screening, antimicrobial tests, antioxidant (DPPH) assay, and in-vitro cytotoxicity against MCF-7 (Breast cancer) cell lines of different plant-...

New antibiotics, antioxidants and anticancer drugs can be found in traditional herbs. Nepal is rich is biodiversity and herbs are being used in Nepal as a source of medicine from ancient time before modern drugs came into existence. In this study, the four medicinal plants were collected from different places of central development region of Nepal:...

Non-thermal plasma is emerging as a promising technology in biology and medicine. In this paper, non-thermal atmospheric pressure Argon plasma jet was produced by applying (10-30) kHz, (0-20) kV AC source. The electrical and optical measurements have been reported to characterize plasma jet for biomedical application. The proposed APAPJ was treated...

The present study was undertaken to find the phytochemical presence, antioxidant potential, antimicrobial activity, and anticancer activity of methanol extract of Berberis Aristata. Plant extract showed the presence of antioxidants like alkaloid, saponin, terpenoids, coumarin, flavonoids, tannin, glycoside and steroid. Further, antioxidant assay li...

The present study was undertaken to find the phytochemical presence, antioxidant potential, antimicrobial activity, and anticancer activity of methanol extract of Berberis Aristata. Plant extract showed the presence of antioxidants like alkaloid, saponin, terpenoids, coumarin, flavonoids, tannin, glycoside and steroid. Further, antioxidant assay li...

Microorganisms harbored by nature and guts of herbivorous animals can degrade different plant related biomass. One of the in-between steps for conversion of lignocellulosic biomass to ethanol entails isolation and identification of microorganisms that could convert pretreated biomass into a suitable form, which could then be fermented into bioethan...

Microorganism found in nature and herbivorous animals harbor a large variety of micro flora that can degrade biomass. We isolated different microflora from hot spring, goat gut, hair spray and wood, on the basis of their ability to secret a wide variety of plant hydrolyzing enzymes. Three different pretreatment strategies (3.35% H 2 SO 4 , NaOH and...

Bioethanol production from lignocellulosic biomass holds remarkable potential to be an alternative source of energy to mitigate current energy demands as well as in reducing greenhouse gas emission. The purpose of this study is to utilize the lignocellulosic biomass for ethanol production and its use as a renewable and alternative source of fossil...

The potential of lignocellulosic biomass as a renewable source of energy and a possible alternative to fossil fuel has attracted most part of the world in recent years. Nepal being agriculture based country, this study intends to utilize the different forms/types of locally available agricultural wastes as the lignocellulosic biomass to produce bio...

Ser315Thr mutations in genes encoding the mycobacteria catalase‐peroxidase (KatG) has been associated with the major resistance to isoniazid (INH) in Mycobacterium tuberculosis (MTB). Also G/C polymorphisms in INT4 region of the solute carrier family 11 member 1 gene (SLC11A1) and susceptibility towards tuberculosis (TB) has been demonstrated world...

The cellular apoptosis is controlled by Bcl-2 family of proteins consisting of both pro-apoptotic and anti-apoptotic members, predominantly residing in mitochondria. Their key function is to control the release of apoptotic factors from mitochondria to cytosol. They also preserve mitochondrial integrity; prevent loss of mitochondrial membrane poten...

Ashwagandha, also called as "Queen of Ayurveda" and "Indian ginseng", is a commonly used plant in Indian traditional medicine, Ayurveda. Its roots have been used as herb remedy to treat a variety of ailments and to promote general wellness. However, scientific evidence to its effects is limited to only a small number of studies. We had previously i...

Mortalin is a chaperone protein that functions in many cellular processes such as mitochondrial biogenesis, intracellular trafficking, cell proliferation and signaling. Its upregulation in many human cancers makes it a candidate target for therapeutic intervention by small molecule drugs. In continuation to our earlier studies showing mortalin as a...

Ashwagandha is regarded as a wonder shrub of India and is commonly used in Ayurvedic medicine and health tonics that claim its variety of health-promoting effects. Surprisingly, these claims are not well supported by adequate studies, and the molecular mechanisms of its action remain largely unexplored to date. We undertook a study to identify and...

Bcl-2 family of proteins consists of both pro apoptotic and anti apoptotic members that control cellular apoptosis. They predominantly reside in mitochondria and control the release of apoptotic factors from mitochondria to the cytosol by regulating its membrane potential and opening of permeability transition pore. Here we report a novel impact of...

Chaperones, particularly the heat-shock proteins, are considered as key players in the maintenance of protein homeostasis and are associated with longevity and cellular immortalization. In this study, we investigated the geroprotective activity of the chemical chaperone glycerol. Glycerol showed significant chaperoning activity in refolding heat-de...