Bertotto Daniela

Bertotto Daniela
University of Padova | UNIPD · Department of Comparative Biomedicine and Food Science BCA

B.Sc, PhD

About

99
Publications
14,712
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1,665
Citations
Citations since 2017
52 Research Items
997 Citations
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2017201820192020202120222023050100150200
2017201820192020202120222023050100150200
2017201820192020202120222023050100150200

Publications

Publications (99)
Conference Paper
Full-text available
In aquaculture, fish undergo a series of events and practices that can compromise their welfare and health. One of the most frequently used stress indicators in fish is the quantification of cortisol in conventional matrices, such as plasma. Nevertheless, blood sampling can be extremely invasive, becoming itself a stressor and cortisol quick increa...
Conference Paper
Full-text available
Aquaculture faces an ongoing threat from bacterial diseases and antibiotic therapy plays a key role in protecting fish. However, the widespread use of antibiotics causes their eventual release into the aquatic environment, with negative implications for the ecosystem. Hence, alternative solutions to reduce the antibiotic load in the water are neede...
Article
Full-text available
This pilot study tested an on-farm protocol based on resource, management, and animal-based measures to evaluate the on-farm health and welfare of rabbits kept in four different housing systems. In detail, the four housing systems were (1) standard breeding cages for reproducing does (3,300 cm2) with their litters associated with bicellular cages f...
Conference Paper
Full-text available
Viral nervous necrosis (VNN) presents a significant threat to the European seabass aquaculture industry, causing extensive losses due to mortality and impaired growth of infected fish. Selective breeding presents an opportunity to develop seabass strains with increased resistance, thus reducing the impact of the disease on the industry. Knowledge o...
Article
Environmental enrichment is a crucial element for the promotion of welfare of animals kept under human care. While a large variety of environmental enrichments has been proposed and studied for terrestrial animals, including a growing area represented by acoustical enrichment such as music, the same is not true for marine mammals. The purpose of th...
Article
Full-text available
In recent decades, the interest in PFAS has grown exponentially around the world, due to the toxic effects induced by these chemical compounds in humans, as well as in other animals and plants. However, current knowledge related to the antistress responses that organisms can express when exposed to these substances is still insufficient and, theref...
Preprint
In recent decades, the interest in PFAS has grown exponentially around the world, due to the toxic effects induced by these chemical compounds in humans, as well as in other animals and in plants. However, current knowledge related to the antistress responses that organisms can express when exposed to these substances is still insufficient and ther...
Article
Full-text available
Triploidy is induced in Atlantic salmon (Salmo salar) to produce sterile fish for genetic containment and to hinder early sexual maturation in farmed fish, but it can have unwanted negative effects on growth, health, and welfare. However, the growth and welfare of triploid fish may be improved by adjusting the rearing environment, feeding condition...
Article
Full-text available
In European sea bass (Dicentrarchus labrax L.), the viral nervous necrosis mortality (MORT), post-stress cortisol concentration (HC), antibody titer (AT) against nervous necrosis virus and body weight (BW) show significant heritability, which makes selective breeding a possible option for their improvement. An experimental population (N = 650) gene...
Article
Full-text available
Background Susceptibility of European sea bass ( Dicentrarchus labrax L.) to viral nervous necrosis (VNN) is well-known. Interest towards selective breeding as a tool to enhance genetic resistance in this species has increased sharply due to the major threat represented by VNN for farmed sea bass and limitations concerning specific therapeutical me...
Article
Full-text available
Fish commercial transport is an ordinary practice in the aquaculture industry. This study aimed to investigate the effect of a 48 h transport stress on stress response of meagre (Argyrosomus regius) juveniles. Radioimmunoassay (RIA) and Real-Time PCR were used to evaluate muscle cortisol levels and to assess glucocorticoid receptor (gr) gene expres...
Article
Full-text available
The study focused on the macroscopic, histological, and biometric analysis of European hake females in GSA 17 (Central-North Adriatic Sea). From 2013 to 2015, 976 females were collected and analyzed monthly. Though females in spawning conditions were found during the whole year, the trend of GSI showed a peak of the reproductive season from April t...
Article
Full-text available
The present study aimed to investigate the acute response of gilthead seabream (Sparus aurata) juveniles exposed to temperature, salinity and ammonia stress. Radioimmunoassay was used to evaluate cortisol levels, whereas insulin-like growth factors (igf1 and igf2), myostatin (mstn), heat-shock protein 70 (hsp70) and glucocorticoid receptor (gr) gen...
Article
Full-text available
In the present study, a multidisciplinary approach was used in order to evaluate growth, muscle development, and stress status in Siberian sturgeon Acipenser baerii larvae at schooling (T1) and complete yolk sac absorption (T2), reared at three stocking densities (low, medium, and high). Larvae growth, morphological muscle development, and whole-bo...
Article
Full-text available
In the present study European sea bass (Dicentrarchus labrax) subjected to two different diets (organic vs. conventional) were evaluated in terms of growing performances, oxidative stress, and contaminant markers. Growing performances were evaluated using biometric measures and condition factor (K), whereas insulin-like growth factor (IGF-I and IGF...
Article
The survival and development of bivalve larvae is adversely impacted by ocean acidification and Vibrio infection, indicating that bivalves need to simultaneously adapt to both stressors associated with anthropogenic climate change. In this study, we use a half-dial breeding design to estimate heritability (h2) for survival to Vibrio harveyi infecti...
Article
Full-text available
Under intensive rearing conditions, the welfare of broiler chickens may be at risk depending on genotype and sex, due to their different growth rates. The practice of quantitative feed restriction may also impact on welfare. This study aimed to evaluate behaviour and corticosterone content in plasma and faeces at different ages using 896 one-day-ol...
Article
Flumequine was nano-immobilized by self-assembly on iron oxide nanoparticles, called surface active maghemite nanoparticles (SAMNs). The binding process was studied and the resulting core-shell nanocarrier ([email protected]) was structurally characterized evidencing a firmly immobilized organic canopy on which the fluorine atom of the antibiotic w...
Article
Environmental temperature is one of the critical factors affecting fish development. The aim of this study was to examine the impact of three different rearing temperatures (16°C, 19°C and 22°C) throughout the endogenous feeding phase of the Siberian sturgeon Acipenser baerii. This was performed by assessing i) larval survival and growth, ii) immun...
Article
Bivalve molluscs are filter-feeders and have a wide range of phytoplankton species as main food source. Some of these species are toxic, producing the so-called phycotoxins, that can get concentrated in edible parts of mussels, oysters, scallops, and clams, causing severe intoxication syndromes in consumers. Since 2008, harmful algae and phycotoxin...
Conference Paper
The aim of this study was to estimate the heritability for mortality after a VNN challenge test in a sea bass experimental population derived from a commercial stock, as well as for cortisol concentration after stress exposure and body weight at a fixed age. Genetic and phenotypic correlations among traits were also estimated, with the aim of ident...
Article
Full-text available
During recent years, research on meat quality in poultry has aimed to evaluate the presence and consequences of breast myopathies as well as the factors which can affect their occurrence by modifying the growth rate. A total of 900 broiler chickens were reared until slaughter (48 D) to evaluate the effect of 2 genetic lines (A vs. B) and feeding pl...
Article
The Venice Lagoon is an interesting example of an ecosystem suffering for a considerable anthropogenic impact, resulting in high concentrations of persistent organic pollutants (POPs) in lagoon sediments and seafood. In this context, biomonitoring is a crucially important task. The present study aimed at evaluating the validity of a multiple biomar...
Article
Full-text available
The ability of peculiar iron oxide nanoparticles (IONPs) to evade the immune system was demonstrated in vivo. The nanomaterial was provided directly into the farming water of zebrafish (Danio rerio) and the distribution of IONPs and the delivery of oxytetracycline (OTC) was studied evidencing the successful overcoming of the intestinal barrier and...
Article
The present study evaluates the effect of dietary barley, based on its potential stress-relieving properties, on rainbow trout under acute stress challenge (hypoxia and crowding) and their recovery. Diets were formulated containing increasing barley concentrations (0, 4, 8, 16, 32%). Cortisol on plasma and fin, glucose and lactate plasma levels and...
Article
Sperm density and performance of wild and farmed male European eels were evaluated to investigate the effect of maintenance in captivity on ejaculate quality. Hormonal stimulation (with human chorionic gonadotropin) lasted for 17 weeks. Different origins of the male European eels partially influenced the quality of the ejaculates. Indeed, wild anim...
Article
Full-text available
Sexual dimorphism is a fascinating subject in evolutionary biology and mostly results from sex-biased expression of genes, which have been shown to evolve faster in gonochoristic species. We report here genome and sex-specific transcriptome sequencing of Sparus aurata, a sequential hermaphrodite fish. Evolutionary comparative analysis reveals that...
Article
In growing rabbits (n = 320; 84 d of age), an increase in the journey duration to the slaughterhouse from 1 h to 3 h under mild climatic conditions (10-13 °C; 75-90% relative humidity) affected several stress indicators in the plasma and muscle collected at slaughter (cortisol; corticosterone; lactate dehydrogenase, LDH; lactate; heat shock protein...
Article
Full-text available
At 33 d of age, 376 crossbred rabbits of both genders were housed in sex-mixed groups in 16 open-top collective pens (1.68 m<sup>2</sup>) in a factorial arrangement, with 2 types of flooring (wooden slats vs. plastic grid, W vs. P) and 2 stocking densities (12 vs. 16 animals/m<sup>2</sup>). Behavioural recordings (time budget), reactivity towards u...
Article
Full-text available
This study evaluates the effects of red beet (RB) and betaine on rainbow trout submitted to an acute stress challenge. A control diet was compared with four experimental diets in which red beet (14 and 28%) and betaine (0.9 and 1.63%) were incorporated in different concentrations according to a factorial design. Cortisol in plasma and fin, glucose...
Article
The Gulf of La Spezia (northern Tyrrhenian Sea, Italy) is a commercially important area both as a shipping port and for mussel farming. Recently, there has been increased concern over environmental disturbances caused by anthropogenic activities such as ship traffic and dredging and the effects they have on the health of farmed mussels. This paper...
Article
Full-text available
Soy isoflavones (the phytoestrogens genistein, daidzein and glycitein) may act as estrogen receptor agonists or antagonists. The aim of this study was to examine the effect of dietary isoflavones on growth, reproduction and health in rainbow trout (Oncorhynchus mykiss). Rainbow trout fed three experimental diets containing different concentrations...
Conference Paper
Full-text available
Behaviour, reactivity and corticosterone levels were evaluated in 376 growing rabbits reared in large groups (20- 27 rabbits per pen) from weaning to slaughter in pens with two floor types (wooden slat, 3 cm between slats, vs. plastic slat, 0.7 cm between slats). Rabbits reared on the wooden floor spent more time resting (68.5% vs. 67.0% total obse...
Article
Full-text available
To evaluate muscle fiber degeneration (MFD) associated with white striping and wooden breast, pectoralis major of 192 broilers differing for genotype (standard vs. high breast yield), gender, and feeding regime (ad libitum vs. restricted rate 80% from 13 to 21 d of age) were sampled at 14, 21, 28, 35, and 46 d of age for histological analyses by he...
Conference Paper
Full-text available
Para evaluar el efecto de la duración del transporte, de la espera antes del sacrificio, y de la posición de las jaulas de transporte en el camión sobre la calidad de la canal y de la carne, se controlaron 160 conejos de 84 días de edad de un cargamento commercial. El aumento de la duración del transporte de 1 a 3 horas aumentó el rendimiento de la...
Article
Full-text available
Due to their importance for the control of meat quality in broiler chickens, the present study aimed at identifying the factors associated with the occurrence of myopathies and characterizing the meat properties when affected by myopathies. To this aim, a total of 768 broiler chickens were reared until slaughter (46 d) to evaluate the effect of gen...
Article
Full-text available
In the present study, the peripheral blood leukocyte phenotypes, lymphocyte subset populations, and oxidative stress parameters were studied in cognitively characterized adult and aged dogs, in order to assess possible relationships between age, cognitive decline, and the immune status. Adult (N = 16, 2-7 years old) and aged (N = 29, older than 8 y...
Article
The aim of this study was to investigate the effects of feeding frequency on post-prandial pattern of circulating glucose, insulin and IGF-I (insulin-like growth factor-I) in European sea bass (D. labrax). Fish (average body weight 72.1 +/- 2 g) were randomly allocated in 12 tanks, reared in a closed thermo regulated recirculating system and fed a...
Article
The present study compared behaviour, fear and stress levels of 456 crossbred rabbits reared in bicellular wire-net cages (2 rabbits/cage) with top and wire floor or open-top collective pens (20 to 54 rabbits/pen) with wooden slatted floor. The effect of pen size (small vs. large) and stocking density (12 vs. 16 rabbits/m2) in collective pens was a...
Article
Benzo(a)pyrene is an important polycyclic aromatic hydrocarbon (PAH) commonly present in the marine environment and responsible for carcinogenic, teratogenic and mutagenic effects in various animal species. In the present study, we investigated by both histochemical and immunohistochemical approaches the effect of an acute exposure to different con...
Article
The aim of this study was to evaluate the histomorphology and the stress response in the bivalve Venerupis philippinarum sampled in four differently polluted sites of the Venice Lagoon (Palude del Monte, Marghera, Ca' Roman and Val di Brenta). This species is often used as bioindicator of environmental pollution since it can bioaccumulate a large v...
Article
Full-text available
A growing number of studies have been showing that dietary probiotics can exert beneficial health effects in both humans and animals. We previously demonstrated that dietary supplementation with Lactobacillus rhamnosus - a component of the human gut microflora - enhances reproduction, larval development, and the biomineralization process in Danio r...
Article
Full-text available
Limited data exist on age-related physiological variations in plasma concentrations of cortisol and dehydroepiandrosterone (DHEA) in dogs, despite their potential role in the pathophysiology of ageing. This study examined plasma cortisol and DHEA concentrations and cortisol/DHEA ratio variations, according to age and sex in 311 dogs, aged from two...
Article
Full-text available
The objective of this work was to investigate the expression of heat shock protein 70 (HSP70) by Western blot (WB) in swine liver. Subsequently, the study aimed to apply this method to two experimental groups of heavy pigs raised in different confinement systems: intensive/indoor (Group A) and extensive/outdoor (Group B). Thirty-six crossbred comme...
Article
Full-text available
The aim of the present work was to evaluate the expression of 8-OHdG (8-hydroxydeoxyguanosine) in the benthic fish Zosterisessor ophiocephalus collected in two differently polluted sites of the Venetian lagoon (Porto Marghera and Caroman). We compared our data on 8-OHdG with those of CYP1A (Cytochrome P450, family 1, subfamily A, polypeptide 1), wh...
Article
Bivalve molluscs, such as Venerupis philippinarum, are often used as bioindicators of environmental pollution since they can bioaccumulate a large variety of pollutants because of their filter feeding. The Polycyclic Aromatic Hydrocarbon (PAH) benzo(a)pyrene (B(a)P) is an important contaminant, commonly present in the marine environment. Pollutants...
Chapter
The temperate aquatic environment is affected by two primary seasonal components, temperature and photoperiod. Many organisms respond to seasonal change physiologically, behaviorally or both. The aim of this study was to investigate the effect of seasonality on cortisol, hematological, and innate immune parameters in European sea bass (Dicentrarchu...
Article
The biometric and nutritional traits of European sea bass from organic or semi-intensive conventional production systems at two commercial sizes (small and medium) were compared. The analysis included a total of 80 specimens. The biometric traits and the texture were not affected by the rearing system, whereas they changed significantly with fish s...
Article
The temperate aquatic environment is affected by two primary components of season, temperature and photoperiod, during the annual cycle. Many organisms respond to seasonal change physiologically, behaviorally or both. The aim of this study was to investigate the effect of seasonality on cortisol, hematological and innate immune parameters in Europe...
Article
Whole body cortisol levels were determined during early larval developmental stages of sea bass (Dicentrarchus labrax) subjected to a heat shock with the aim to investigate the correlation between the stress event and the activation of the hypothalamic-pituitary-interrenal axis. Moreover, the mRNA expression of inducible heat shock protein 70 (HSP7...
Article
Sex and stage of gonad maturity in 6 year-old sturgeon hybrids (Acipenser naccarii female × Acipenser baerii male) were examined by means of ultrasonography, histology and sex steroid analyses during the reproductive season. Ultrasound images of gonads revealed male and female sturgeons at different stages of maturity, distinguished by gonad morpho...
Article
Several studies carried out in the last years have demonstrated the presence of a wide range of contaminants in some areas of the Venice Lagoon. Many of these contaminants are able to drive free radical reactions, which lead to oxidative stress and can potentially affect fish health. In the present study, oxidative stress biomarkers were examined i...
Article
Full-text available
The cellular localization of IGF-I, IGF-II and MSTN proteins was investigated during ontogenesis of triploid sea bass (Dicentrarchus labrax) by an immunohistochemical approach. The results were compared with those observed in diploids. IGF-I immunostaining was mainly observed in skin, skeletal muscle, intestine and gills of both diploids and triplo...