Bernardo Gonzalez

Bernardo Gonzalez
Adolfo Ibáñez University · Facultad de Ingeniería y Ciencias

PhD

About

246
Publications
15,844
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3,980
Citations
Additional affiliations
March 2009 - present
January 1989 - March 2009
Pontifical Catholic University of Chile

Publications

Publications (246)
Article
Background and Aims Nitrogen (N) is essential for plant growth, yet the role of diazotrophic bacteria in non-nodulating plants, particularly from the β-class Pseudomonadota, remains unclear. We explored the mechanisms underlying the interaction of Cupriavi- dus taiwanensis LMG19424, belonging to this group and known for nodulation induction in Mimo...
Article
Full-text available
Background and Aims Nitrogen (N) is essential for plant growth, yet the role of diazotrophic bacteria in non-nodulating plants, particularly from the β-class Pseudomonadota, remains unclear. We explored the mechanisms underlying the interaction of Cupriavidus taiwanensis LMG19424, belonging to this group and known for nodulation induction in Mimosa...
Article
Full-text available
Introduction This work investigates whether rhizosphere microorganisms that colonize halophyte plants thriving in saline habitats can tolerate salinity and provide beneficial effects to their hosts, protecting them from environmental stresses, such as aromatic compound (AC) pollution. Methods To address this question, we conducted a series of expe...
Article
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The self‐sufficient cytochrome P450 RhF and its homologues belonging to the CYP116B subfamily have attracted considerable attention due to the potential for biotechnological applications based in their ability to catalyse an array of challenging oxidative reactions without requiring additional protein partners. In this work, we showed for the first...
Article
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Perchlorate is an oxidative pollutant toxic to most of terrestrial life by promoting denaturation of macromolecules, oxidative stress and DNA damage. However, several microorganisms, especially hyperhalophiles, are able to tolerate high levels of this compound. Furthermore, relatively high quantities of perchlorate salts were detected on the Martia...
Article
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Synthetic microbial communities (SynComs) are a useful tool for a more realistic understanding of the outcomes of multiple biotic interactions where microbes, plants, and the environment are players in time and space of a multidimensional and complex system. Toward a more in-depth overview of the knowledge that has been achieved using SynComs in th...
Article
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Plants must deal with harsh environmental conditions when colonizing abandoned copper mine tailings. We hypothesized that the presence of a native microbial community can improve the colonization of the pioneer plant, Baccharis linearis , in soils from copper mining tailings. Plant growth and microbial community compositions and dynamics were deter...
Preprint
Full-text available
Nitrogen (N) is an essential macronutrient for plants. Some plant species obtain this nutrient by interacting with N-fixing bacteria. These beneficial interactions are well described in legumes but have also been observed in non-legume plant species that are unable to form root nodules. We studied the expanding role of beneficial plant-bacteria int...
Article
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Plant defense responses to biotic stresses are complex biological processes, all governed by sophisticated molecular regulations. Induced systemic resistance (ISR) is one of these defense mechanisms where beneficial bacteria or fungi prime plants to resist pathogens or pest attacks. In ISR, the defense arsenal in plants remains dormant and it is on...
Article
Quorum-sensing systems play important roles in host colonization and host establishment of Burkholderiales species. Beneficial Paraburkholderia species share a conserved quorum-sensing (QS) system, designated BraI/R, that controls different phenotypes. In this context, the plant growth-promoting bacterium Paraburkholderia phytofirmans PsJN possesse...
Article
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Several bacteria use the plant hormone indole-3-acetic acid (IAA) as a sole carbon and energy source. A cluster of genes (named iac ) encoding IAA degradation has been reported in Pseudomonas putida 1290, but the functions of these genes are not completely understood. The plant growth-promoting rhizobacterium Paraburkholderia phytofirmans PsJN harb...
Article
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Cupriavidus pinatubonensis JMP134, like many other environmental bacteria uses a range of aromatic compounds as carbon sources. Previous reports have shown the preference for benzoate when this bacterium grows on binary mixtures composed of this aromatic compound and 4-hydroxybenzoate or phenol. However, this observation has not been extended to ot...
Book
Full-text available
It becomes increasingly clear that the basis of antibiotic resistance problem among bacterial pathogens is not confined to the borders of clinical microbiology but has broader ecological and evolutionary associations. This Research Topic “Role and prevalence of antibiosis and the related resistance genes in the environment” in Frontiers in Microbio...
Article
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Plant growth-promoting rhizobacteria (PGPR) induce positive effects in plants, such as increased growth or reduced stress susceptibility. The mechanisms behind PGPR/plant interaction are poorly understood, as most studies have described short-term responses on plants and only a few studies have analyzed plant molecular responses under PGPR coloniza...
Data
Lineal regression between rosette area and days after sowing, under the different treatments. Data were Log10 transformed, and each circle or triangle represents data from one plant. (TIF)
Data
Examples of up-regulated genes under PsJN treatment classified in different functional categories*. (DOCX)
Data
Examples of down-regulated genes under PsJN treatment classified in different functional categories*. (DOCX)
Data
Molecular functions affected by strain PsJN and K-PsJN treatments. Molecular functions of the up-regulated genes or down-regulated genes under the different. (TIF)
Data
Changes in gene expression in PsJN and K-PsJN-treated plants. Venn diagrams of up-regulated and down-regulated genes in complete plants of 4 rosette leaves stages under PsJN or K-PsJN treatments. The intersections show the number of genes that are. (TIF)
Data
Complete list of genes that are regulated by K-PsJN treatment. (XLSX)
Data
Complete list of genes that are regulated by PsJN treatment. (XLSX)
Data
List of real time RT-PCR primers. Melting temperature and references, if applicable, are indicated. (DOCX)
Article
Full-text available
Although not fully understood, molecular communication in the rhizosphere plays an important role regulating traits involved in plant-bacteria association. Burkholderia phytofirmans PsJN is a well-known plant growth promoting bacterium, which establishes rhizospheric and endophytic colonization in different plants. A competent colonization is essen...
Article
We studied the abundance and diversity of the sulfate-reducing prokaryotes (SRPs) in two 30-cm marine chilean sediment cores, one with a long-term exposure to copper-mining residues, the other being a non-exposed reference sediment. The abundance of SRPs was quantified by qPCR of the dissimilatory sulfite reductase gene β-subunit (dsrB) and showed...
Article
Aims This work addresses the relevant effects that one single compound, used as model herbicide, provokes on the activity/survival of a suitable herbicide degrading model bacterium and on a plant that hosts this bacterium and its bacterial rhizospheric community. Methods The effects of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D), on Acaci...
Article
Full-text available
The role of broad-host range IncP-1ε plasmids in the dissemination of antibiotic resistance in agricultural systems has not yet been investigated. These plasmids were detected in total DNA from all of 16 manure samples and in arable soil based on a novel 5′-nuclease assay for real-time PCR. A correlation between IncP-1ε plasmid abundance and antibi...
Article
Full-text available
Plant rhizosphere and internal tissues may constitute a relevant habitat for soil bacteria displaying high catabolic versatility towards xenobiotic aromatic compounds. Root exudates contain various molecules that are structurally related to aromatic xenobiotics and have been shown to stimulate bacterial degradation of aromatic pollutants in the rhi...
Article
Copper has a dual role for organisms, both as micronutrient and toxic element. Copper mining activities have an enormous ecological impact because of the extraction process and the consequent release of copper-containing waste materials to the environment. In northern Chile, mainly in the Chañaral coastal area, this phenomenon is clearly evident. T...
Article
The relevance of the β-proteobacterial Burkholderiales order in the degradation of a vast array of aromatic compounds, including several priority pollutants, has been largely assumed. In this review, the presence and organization of genes encoding oxygenases involved in aromatics biodegradation in 80 Burkholderiales genomes is analysed. This genomi...
Article
As other environmental bacteria, Cupriavidus necator JMP134 uses benzoate as preferred substrate in mixtures with 4-hydroxybenzoate, strongly inhibiting its degradation. The mechanism underlying this hierarchical use was studied. A C. necator benA mutant, defective in the first step of benzoate degradation, is unable to metabolize 4-hydroxybenzoate...
Article
Full-text available
Nitrogen (N) is the mineral nutrient required in the greatest amount and its availability is a major factor limiting growth and development of plants. As sessile organisms, plants have evolved different strategies to adapt to changes in the availability and distribution of N in soils. These strategies include mechanisms that act at different levels...
Article
Full-text available
Cigarette smokers and those exposed to second hand smoke are more susceptible to life threatening infection than non-smokers. While much is known about the devastating effect tobacco exposure has on the human body, less is known about the effect of tobacco smoke on the commensal and commonly found pathogenic bacteria of the human respiratory tract,...
Article
Full-text available
The eastern tropical Pacific Ocean holds two of the main oceanic oxygen minimum zones of the global ocean. The presence of an oxygen-depleted layer at intermediate depths, which also impinges on the seafloor and in some cases the euphotic zone, plays a significant role in structuring both pelagic and benthic communities, and also in the vertical pa...
Article
Phenoxyalkanoic herbicides such as 2,4-dichlorophenoxyacetate (2,4-D), 2,4-dichlorophenoxybutyrate (2,4-DB) or mecoprop are widely used to control broad-leaf weeds. Several bacteria have been reported to degrade these herbicides using the α-ketoglutarate-dependent, 2,4-dichlorophenoxyacetate dioxygenase encoded by the tfdA gene, as the enzyme catal...
Article
To develop a real-time PCR-based strategy for the detection of Paenibacillus larvae vegetative cells and spores to improve the diagnosis and the screening of American foulbrood (AFB), the most harmful pathology of honeybee brood. A real-time PCR that allowed selective identification and quantification of P. larvae 16S rRNA sequence was developed. U...
Data
Functional distribution of unique genes. COG categories are as follows: Information storage and processing: A, RNA processing, modification; B, chromatin structure; J, translation, ribosomal structure/biogenesis; K, transcription; L, DNA replication, recombination, repair. Cellular processes: D, cell division, chromosome partitioning; M, cell envel...
Data
Functional annotation of key metabolic genes of C. necator JMP134. (0.27 MB DOC)
Article
Full-text available
Cupriavidus necator JMP134 is a Gram-negative beta-proteobacterium able to grow on a variety of aromatic and chloroaromatic compounds as its sole carbon and energy source. Its genome consists of four replicons (two chromosomes and two plasmids) containing a total of 6631 protein coding genes. Comparative analysis identified 1910 core genes common t...
Article
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This study reports on the factors involved in regulating the composition and structure of bacterial communities epiphytic on intertidal macroalgae, exploring their temporal variability and the role of copper pollution. Culture-independent, molecular approaches were chosen for this purpose and three host species were used as models: the ephemeral Ul...
Article
Full-text available
In the last decades, the worldwide increase in copper wastes release by industrial activities like mining has driven environmental metal contents to toxic levels. For this reason, the study of the biological copper-resistance mechanisms in natural environments is important. Therefore, an appropriate molecular tool for the detection and tracking of...
Article
Bioremediation is an important technology for the removal of persistent organic pollutants from the environment. Bioaugmentation with the encapsulated Pseudomonas sp. strain MHP41 of agricultural soils contaminated with the herbicide simazine was studied. The experiments were performed in microcosm trials using two soils: soil that had never been p...
Article
This study used culture-dependent and culture-independent approaches to characterize bacterial communities in copper plumbing corrosion and to assess biofilm formation and copper resistance of heterotrophic bacteria isolated from copper pipes. Water and copper pipes were collected from a cold-water household distribution system affected by 'blue wa...
Article
Sulfate-reducing bacterial communities from coastal sediments with a long-term exposure to copper (Cu)-mining residues were studied in lactate enrichments. The toxicity of excess copper may affect sulfate-reducing bacterial communities. Sulfate reduction was monitored by sulfate and organic acid measurements. Molecular diversity was analyzed by 16S...
Article
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Catechols are central intermediates in the metabolism of aromatic compounds. Degradation of 4-methylcatechol via intradiol cleavage usually leads to the formation of 4-methylmuconolactone (4-ML) as a dead-end metabolite. Only a few microorganisms are known to mineralize 4-ML. The mml gene cluster of Pseudomonas reinekei MT1, which encodes enzymes i...
Article
Full-text available
Maleylacetate reductases (MAR) are required for biodegradation of several substituted aromatic compounds. To date, the functionality of two MAR-encoding genes (tfdF(I) and tfdF(II)) has been reported in Cupriavidus necator JMP134(pJP4), a known degrader of aromatic compounds. These two genes are located in tfd gene clusters involved in the turnover...
Article
Cupriavidus necator JMP134(pJP4) is able to grow on 3-chlorobenzoate (3-CB), a model chloroaromatic pollutant. Catabolism of 3-CB is achieved via the expression of the chromosomally encoded benABCD genes and the tfd genes from plasmid pJP4. Since passive diffusion of benzoic acid derivatives at physiological pH is negligible, the uptake of this com...
Article
Full-text available
Cupriavidus necator JMP134 has been extensively studied because of its ability to degrade chloroaromatic compounds, including the herbicides 2,4-dichlorophenoxyacetic acid (2,4-D) and 3-chlorobenzoic acid (3-CB), which is achieved through the pJP4-encoded chlorocatechol degradation gene clusters: tfdCIDIEIFI and tfdDIICIIEIIFII. The present work de...