Bernardo Dias Ribeiro

• Federal University of Rio de Janeiro

Sargassum filipendula is a marine macroalgae, also known as brown algae. These species contain significant amounts of polysaccharides, such as alginates, and phenolic compounds, including phlorotannins, with excellent biological properties. This study evaluated the extraction of bioactive compounds from the residual biomass of Sargassum filipendula...

Plastic waste management is a major global concern as the production of these materials continues to increase without proportional advancements in recycling technologies. Dissolution‐precipitation is a promising emerging technology, offering a simple yet effective process to manage complex materials that often go unrecycled, while also recovering h...

Yeast biomass, a by-product of various industrial processes, is a sustainable source of food ingredients. Despite its nutritional richness, studies on Yarrowia lipolytica W29 biomass for high-value compound production using low-cost substrates like glycerol and glucose remain limited. These substrates enhance productivity and modulate cell wall com...

This study investigates the partial purification, characterization, and application of lipase from Yarrowia lipolytica IMUFRJ50682, produced via solid-state fermentation (SSF), for omega-6 enrichment through fish oil hydrolysis. Lipase production utilized a fermentation substrate composed of soybean meal and andiroba cake (1:1) and was partially pu...

Heavy crude oil reserves are characterized by their high viscosity and density, largely due to significant quantities of asphaltenes. The removal of asphaltene precipitates from oil industry installations is crucial, as they can contaminate catalysts and obstruct pipelines. Therefore, this study aimed to bio-transform heavy oil asphaltenes into sma...

Purple grape juice produces a significant amount of grape pomace (GP) as a by-product, which can be reused as a raw material in producing craft beers with bioactive properties. The objective of this study was to produce craft beers with the addition of GP during the fermentation process to evaluate the incorporation of bioactive compounds, aiming a...

Orange and coffee grounds by-products, rich in phenolic bioactive compounds, can be used in the food industry as antioxidants, colorants, flavorings and additives, mainly because they are solvents that are easy to prepare, have a lower cost, are thermally stable, biodegradable, renewable, and are considered GRAS (Generally Recognized as Safe). Deep...

This work aimed to evaluate the lipases from Yarrowia lipolytica as a biocatalyst for synthesizing fatty acid ethyl esters (FAEE). The Yarrowia lipolytica lipases were evaluated in three distinct forms: firstly, as the crude enzymatic liquid extract derived from the submerged fermentation process of Y. lipolytica (YLL); secondly, as YLL immobilized...

Plant-based milk has gained considerable attention; however, its high nutritional variation highlights the need for improved formulation designs to enhance its quality. This study aimed to nutritionally compare cow milk with plant-based milk produced from hazelnuts (H), Brazil nuts (BN), cashew nuts (CN), soybeans (S), and sunflower seeds (SS), and...

Separating hydroalcoholic mixtures remains a significant challenge in engineering. Liquid–liquid extraction has emerged as an appealing alternative method, because it avoids the need for the large energy inputs, volatile organic compounds, and high pressures that are typically required by other separation processes. This study explores the use of h...

This study aimed to evaluate the sequential hydrolysis of the biomass from unconventional and versatile Y. lipolytica to recover mannoproteins, carbohydrates, and other compounds as well as to determine the antioxidant activity of ultrafiltered fractions. The crude biomass underwent autolysis, and the resulting supernatant fraction was used for man...

The use of enzymes to hydrolyze the plant cell matrix is a method known for extracting bioactive substances. The current work used this strategy to produce a rose petal extract rich in anthocyanins that is stable in the presence of marine polysaccharides and has a high antioxidant activity. The process evaluation was carried out sequentially, initi...

Plastic pollution has been a topic of grave concern worldwide due to major environmental, economic, health, and social impacts. Nevertheless, our society is far from casting plastics aside since they are essential for several applications (e.g. food packaging, automotive, electronics, building, and construction). Poly (terephtalate ethylene) (PET),...

The traditional consumer society based on oil and petroleum products has increased the number of cases of total petroleum hydrocarbon (TPH) soil contamination worldwide. It is vital to proceed with the treatment and requalification of these affected areas using cost-effective and environmentally sustainable methods. This work presents a sustainable...

Estrogens are emergent pollutants found in low levels in water bodies, and for this reason they require concentration for quantification. In this study, aqueous two‐phase systems (ATPSs) were used as an alternative strategy to improve hormone detection and quantification from real water samples. In addition, a predictive computational method (COSMO...

Polyolefins comprise some of the most abundant plastics, but their recycling faces several difficulties. This work focuses on the recycling of high-density polyethylene (HDPE) through the dissolution-precipitation method. Although few...

Citation: Buarque, F.S.; Farias, M.A.; Sales, J.C.S.; Carniel, A.; Ribeiro, B.D.; Lopes, V.R.d.O.; Castro, A.M.; Coelho, M.A.Z. Valorization of Macuba (Acronia aculeata) for Integrated Production of Lipase by Yarrowia lipolytica and Biodiesel Esters. Fermentation 2023, 9, 992. Abstract: Enzymatic biodiesel production is a potential eco-friendly alt...

Babassu (Orbignya phalerata) is a valuable oleaginous fruit in Brazil’s economy. After processing to extract oil, large quantities of residual mesocarp are generated. Typically, this by-product finds reuse as an additive in animal feed or is sold as flour for human consumption. However, there is a pressing need for innovative approaches to valorize...

Aqueous two-phase systems (ATPS) are considered efficient and sustainable downstream processing techniques in extracting and separating enzymes. In this work, we evaluated the use of ATPS based on quaternary ammo-nium (tetrabutylammonium bromide ([N 4444 ]Br), tetrabutylammonium chloride ([N 4444 ]Cl), choline chloride (ChCl), and betaine) + potass...

The chemical recycling of poly(ethylene terephthalate) (PET) residues was performed via glycolysis with ethylene glycol (EG) over Mg-Fe and Mg-Al oxide catalysts derived from layered double hydroxides. Catalysts prepared using the high supersaturation method (h.s.c.) presented a higher surface area and larger particles, but this represented less PE...

Cellulases are a class of enzymes of great industrial interest that present several strategic applications. However, the high cost of enzyme production, coupled with the instabilities and complexities of proteins required for hydrolytic processes, still limits their use in several protocols. Therefore, enzyme immobilization may be an essential tool...

Yeasts are used to produce several bioproducts, including functional bio-molecules, enzymes, biofuels, lipids, pigments, vitamins, organic acids, and other value-added bioproducts. When the production of the bioproduct occurs intracellularly, methods of disruption are traditionally used (mechanical and non-mechanical), which promote the release of...

Green chemistry and engineering are potential alternatives for achieving higher sustainability and lower generation of hazardous compounds in chemical product design, production, and use. Deep Eutectic Solvents (DES) are characterized as green solvents and have become increasingly attractive due to their characteristic design solvents. In this work...

Efforts have been concentrated on developing alternative methods of enzyme purification that are less costly and highly efficient. In this work, we evaluated three different methods for lipase purification from Yarrowia lipolytica, such as precipitation using ammonium sulfate, ethanol, or acetone; aqueous two-phase systems (ATPS) based on polyethyl...

Increasing exploration of natural sources and the development of new oil-derived technologies have caused environmental contamination. Thus, among the bioremediation strategies, biostimulation and bioaugmentation techniques have been the most used in the current literature. This study aimed to build a bench-scale bioreactor to promote degradation u...

The production of polyunsaturated fatty acids from fish oil, which is related to various health benefits including effects against cardiovascular diseases, antihypertensive, anticancer, antioxidant, antidepression, anti-aging, and anti-arthritis effects, among others, can be advantageously performed through the application of lipase. However, the h...

1,3-Propanediol (PDO) can be produced by fermentation of glycerol, but the post-fermentation separation of the PDO from the aqueous medium is hampered by the low volatility and high hydrophilicity of PDO. A new method for separating hydrophilic products from water is proposed, called Solvent-Assisted Switchable Water (SASW). In this method, a hydro...

Aqueous two-phase systems (ATPS) are considered efficient and sustainable downstream processing techniques in extracting and separating enzymes. In this work, we evaluated the use of ATPS based on quaternary ammonium (tetrabutylammonium bromide ([N4444]Br), tetrabutylammonium chloride ([N4444]Cl), choline chloride (ChCl), and betaine) + potassium p...

Aqueous two-phase systems (ATPS) based on ionic liquids (IL) and deep eutectic solvents (DES) are ecofriendly choices and can be used to selectively separate compounds of interest, such as bioactive compounds. Bioactive compounds are nutrients and nonnutrients of animal, plant, and microbial origin that benefit the human body in addition to their c...

Several microorganisms have been reported as capable of acting on poly(ethylene terephthalate) (PET) to some extent, such as Yarrowia lipolytica, which is a yeast known to produce various hydrolases of industrial interest. The present work aims to evaluate PET depolymerization by Y. lipolytica using two different strategies. In the first one, bioca...

Extractive fermentation is an efficient downstream process to segregate the desired product in a fermentative process. Here, an integrated extractive fermentation process with an aqueous two-phase system (ATPS) was applied to evaluate the lipase and citric acid production by Yarrowia lipolytica. For this purpose, the influences of polyethylene glyc...

Bioactive compounds can provide health benefits beyond the nutritional value and are originally present or added to food matrices. However, because they are part of the food matrices, most bioactive compounds remain in agroindustrial by-products. Agro-industrial by-products are generated in large quantities throughout the food production chain and...

Plant-based milk substitutes, or simply plant milk, are produced from the breakage of the interesting raw material, reducing its size, with subsequent extraction in water and homogenization. Soymilk is still the most consumed, but there is a variety of options for vegetable milk production. The raw materials used can be classified into cereals, leg...

Since plastic pollution emerged as an urgent environmental problem, different biocatalysts have been tested for poly(ethylene terephthalate) (PET) hydrolysis. This work evaluated three different possible inducers for lipases and/or esterases, two natural sources of biopolymers (apple peels and commercial cork) and PET, as supplements in the solid-s...

BACKGROUND Every minute, 1 million bottled drinks were purchased worldwide in 2017. Poly(ethylene terephthalate) (PET) bottles are highly recalcitrant wastes, taking at least 450 years to decompose naturally, and their accumulation in the environment triggers a series of environmental impacts. Recycling is an environmentally friendly alternative to...

The aqueous extract of Joazeiro stem bark (EJSB) and its high molecular weight fraction (HMWF) were examined as potential corrosion inhibitors of mild steel in 1 mol L⁻¹ hydrochloric acid media, using weight-loss measurements, potentiodynamic polarization curves and an electrochemical impedance spectroscopy (EIS).Varying the concentration of the in...

The aim of the present research was to develop green and sustainable extraction procedure for β-carotene recovery from pumpkin. A series of hydrophobic natural deep eutectic solvents (NADESs) based on fatty acids were prepared to establish high extraction efficiency of β-carotene and to increase stability of extracted carotenoids from the pumpkin....

Azo dyes are widely used in the textile industry due to their resistance to light, moisture, and oxidants. They are also an important class of environmental contaminant because of the amount of dye that reaches natural water resources and because they can be toxic, mutagenic, and carcinogenic. Different technologies are used for the decolorization...

Aiming at providing new solution for processing and upcycling PET, the complete solubilization of PET from water bottle in natural eutectic solvents is here demonstrated as well as its full...

Biotechnology and Bioengineering techniques have been widely used in the production of biofuels, chemicals, pharmaceuticals and food additives, being considered a "green" form of production because they use renewable and non-polluting energy sources. On the other hand, in the traditional processes of production, the target product obtained by biote...

The aim of this work is to develop an optimized enzymatic assisted extraction methodology to extract carotenoids and phenolic compounds from sunflower wastes (petals and florets) using natural hydrophobic green solvents. Several natural green hydrophobic solvents were used as well as natural hydrophobic eutectic solvents composed of d,l-menthol and...

Yarrowia lipolytica is a yeast that presents high biotechnological potential due to its ability to produce many metabolites, among them lipases and esterases, which are important industrial biocatalysts. Since Brazil is an agroindustrial country, it generates an enormous diversity of residues or byproducts that can be used as a platform for biomole...

Yarrowia lipolytica IMUFRJ 50,682 is a Brazilian wild-type strain with potential application in bioconversion processes which can be improved through synthetic biology. In this study, we focused on a combinatorial dual cleavage CRISPR/Cas9-mediated for construction of irreversible auxotrophic mutants IMUFRJ 50,682, which genomic information is not...

A growing number of consumers opt for plant-based milk substitutes for medical reasons, like cow's milk protein allergy (CMPA), lactose intolerance (LI), or as a lifestyle choice. Plant-based milk substitutes, or plant extracts, are water-soluble extracts of legumes, oilseeds, cereals or pseudocereals that resemble bovine milk in appearance. It is...

Yarrowia lipolytica lipase obtained by solid-state fermentation was characterized and applied in the synthesis of esters with commercial value in the food industry. The effect of different conditions on the hydrolysis activity of this biocatalyst was evaluated in the presence of metal ions, solvents, detergents, several pH and temperature parameter...

The occurrence of micropollutants in aquatic ecosystems poses a serious public health and environmental problem. For that purpose, the main goal of this work is the removal of an endocrine disruptor micropollutant compound, Bisphenol-A (BPA), from aqueous environments using hydrophobic deep eutectic solvents (DES). In particular, DES composed of DL...

BACKGROUND Eutectic solvents are a mixture of two compounds which possess a lower melting temperature than compounds by themselves at low temperatures (below 100ºC), using quaternary ammonium salts, as choline and betaine, and organic acids, polyols and amides as hydrogen bond donors. These solvents can be an alternative as non-aqueous media for en...

This study aimed to evaluate the use of a lyophilized fermented solid (named solid enzymatic preparation, SEP), with lipase activity, as a low-cost biocatalyst for esterification reactions of fatty acids present in acid raw materials for biodiesel synthesis. The SEP was obtained by solid-state fermentation (SSF) of soybean bran using the strain of...

In the last couple of years, deep eutectic solvents (DESs) have been raising a lot of attention mainly due to their versatility and their easy and speedy preparation without the need of further purification. Moreover, the vast array of very different compounds that can be selected for their preparation has led to the full tailoring of their relevan...

Background: The proposal to perform scale-down of culture systems (2 to 4 mL) could be more efficient for screen multiple formulations and operational conditions. For such, is important to evaluate if the kinetic parameters of a bioprocess are comparative to conventional lab-scale reactors. In the present study, the effects of different miniaturize...

Two new phthalic salts, dicholine terephthalate ([Ch]2[Ter]) and dicholine isophthalate ([Ch]2[Iso]) were synthesized in one-pot reactions. After structures confirmation by 13C and 1H NMR and their characterization (viscosity, solubility, thermal analysis), [Ch]2[Iso] was classified as an ionic liquid, whereas [Ch]2[Ter] was not, revealing a role o...

The vegetable oil industry generates several materials after oil extraction that can be used as animal feed but that can represent an environmental and public health concern in case of improper disposal. However, the bioconversion of these feedstocks for the production of enzymes of industrial interest, such as lipases, by fermentation is an intere...

Due to the actual emphasis in revalorization of agro-industrial wastes and cost reduction of enzymes production, this work aimed the optimization of lipase production from different oil cakes (OCs) produced in Brazil, by solid-state fermentation using Aspergillus ibericus MUM 03.49 and the application of the produced lipase in hydrolysis and esteri...

O estudo trata sobre o desenvolvimento de emulsão cosmética utilizando as saponinas de Juá e Sisal e a avaliação dos mecanismos de envelhecimento ao longo do tempo utilizando o processamento digital de imagem como ferramenta. Para o estudo da estabilidade física das emulsões foram mensurados o diâmetro médio das gotículas de óleo a cada 7 dias dura...

White biotechnology is the use of enzymes and microorganisms in industrial production through applied biocatalysis. This allows for milder reaction conditions (pH and temperature) and the use of more environmentally-compatible catalysts and solvents. This, in turn, leads to processes which are shorter, generate less waste, making them both environm...

White biotechnology is the use of enzymes and microorganisms in industrial production through applied biocatalysis. This allows for milder reaction conditions (pH and temperature) and the use of more environmentally-compatible catalysts and solvents. This, in turn, leads to processes which are shorter, generate less waste, making them both environm...

White biotechnology is the use of enzymes and microorganisms in industrial production through applied biocatalysis. This allows for milder reaction conditions (pH and temperature) and the use of more environmentally-compatible catalysts and solvents. This, in turn, leads to processes which are shorter, generate less waste, making them both environm...

White biotechnology is the use of enzymes and microorganisms in industrial production through applied biocatalysis. This allows for milder reaction conditions (pH and temperature) and the use of more environmentally-compatible catalysts and solvents. This, in turn, leads to processes which are shorter, generate less waste, making them both environm...

Inspired by one of the major problems in the pharmaceutical industry, we advantageously used the formation of eutectic mixtures to synthesize new solvents. The aim of this work is to identify low viscosity, cheap, biodegradable and hydrophobic eutectic solvents from natural resources. Consequently, novel eutectic mixtures based on L-menthol and nat...

Considering the potential applications of ionic liquids (ILs) as solvents in biotechnological processes such as food production via microbial synthesis, the work presented here aimed to evaluate the toxicity of these new solvents to microorganisms of interest in the food industry. Following the international standard method of the CLSI (Clinical an...

Sisal (Agave sisalana) is the main hard fiber produced worldwide, with an estimated generation of 400 thousands t in 2011. From its leaves, only the hard fibers, which represent 3–5% of their weight, are removed. The remaining 95–97% is referred to as sisal waste and contains steroidal saponins, that can be potentially used in foods, cosmetics and...

Saponins are triterpenic or steroidal glycosides present in many plants, as juá (Ziziphus joazeiro) and sisal (Agave sisalana), representatives of the Brazilian agrobiodiversity. Due to their properties, saponins are finding application in fine chemistry and pharmaceutical industry. Thus, the study of the extraction of these components from their n...

Saponins, molecules classified as triterpenic or steroidal glycosides, are metabolites distributed in all the plant kingdom that can be used for the production of foods, cosmetics, and pharmaceuticals, as well as in soil bioremediation. Saponins are normally extracted from natural resources with water, ethanol and/or methanol, and then concentrated...

Flaxseed (Linum usitatissimum) meal, the main byproduct of the flaxseed oil extraction process, is composed mainly of proteins, mucilage, and phenolic compounds. The extraction methods of phenolics either commonly employed the use of mixed solvents (dioxane/ethanol, water/acetone, water/methanol, and water/ethanol) or are done with the aid of alkal...

Currently, several groups of enzymes belonging to the classes of oxidoreductases and hydrolases have been applied as clean alternatives for many processes in food, detergents, textiles and pharmaceutical industries. The objective of this article is to present new approaches for undergraduate experimental classes based on simple and rapid methodolog...

Extracts from plant tissue are a rich source of lead compounds for nutraceutical or pharmaceutical applications. Nevertheless, the concentration of added value compounds in the plants extracts is usually lower than 1–2%. As consequence, a search for new improved, more efficient technologies combined with solvent engineering has emerged in the last...

Carotenoids, precursors of vitamin A, are natural pigments synthesized by plants and some microorganisms. Humans and animals are not able to synthesize and need to acquire them by alimentation. Beyond the health benefits promoted by vitamin A, these compounds have antioxidant capacity which gives protection for cardiovascular and degenerative disea...

Juá (Ziziphus joazeiro) is a Brazilian plant and its bark has been used as a detergent and phytotherapic due to its high saponin content (2–10 %). Saponins are triterpenic glycosides with some properties that aid their use in food, cosmetic and pharmaceutical industries. The object of the present work was to develop an extraction and concentration...

As bebidas sabor guaraná são muito populares no Brasil e têm apresentado um excelente potencial de vendas no mercado externo. De acordo com as leis brasileiras, bebidas sabor guaraná devem conter entre 0,02g a 0,2g de semente de guaraná ou equivalente, para cada 100mL de produto. Tais teores são usualmente obtidos pela adição de um extrato concentr...

The present work aimed at the extraction and concentration of β-carotene from crude and refined buriti oils using enzymatic hydrolysis as a process strategy. The performance of two commercial lipolytic preparations (Lipozyme TL IM and CALB L), as well as lipases from Yarrowia lipolytica, was evaluated. The parameters considered in the hydrolysis pr...

β-carotene is the main source of pro-vitamin A and is widely used as a food colorant, with a global market estimated to surpass USD 280 million in 2015. The majority of the β-carotene commercialized in the world is obtained by chemical synthesis from β-ionone. Alternatively, the production of β-carotene can be reached on a biotechnological basis, u...