Bernard Wone

Bernard Wone
University of South Dakota | USD · Department of Biology

About

57
Publications
6,266
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
920
Citations
Additional affiliations
October 2012 - August 2015
University of Nevada, Reno
Position
  • PostDoc Position
Description
  • Engineering the CAM machinery into a C3 biofuel plant.
July 2011 - July 2012
The University of Arizona
Position
  • PostDoc Position
Description
  • Investigated the trade-off between flight and fecundity

Publications

Publications (57)
Article
Full-text available
Addiction behavior and the resulting short-term or long-term disabilities continue to increase globally, especially during the current COVID pandemic. We analyze how national measures of 38 global indices correlate with national addiction-related disability rates resulting from four primary addictive substances: alcohol, tobacco, drugs, and food. W...
Article
Full-text available
Seasonal changes, such as alterations in food availability or type and cold conditions, present challenges to free-living birds living in highly seasonal climates. Small birds respond to such challenges through seasonal metabolic flexibility, which better matches seasonal metabolic phenotypes to environmental conditions and can improve fitness. To...
Article
Abiotic stresses have the greatest impact on the growth and productivity of crops, especially under current and future extreme weather events due to climate change. Thus, it is vital to explore novel strategies to improve crop plant abiotic stress tolerance to feed an ever-growing world population. Selaginella lepidophylla is a desiccation-tolerant...
Article
Cactus pear (Opuntia ficus-indica) is a high productivity species within the Cactaceae grown in many semiarid parts of the world for food, fodder, forage, and biofuels. O. ficus-indica utilises obligate crassulacean acid metabolism (CAM), an adaptation that greatly improves water-use efficiency (WUE) and reduces crop water usage. To better understa...
Article
Full-text available
Plant genetic engineering will be essential to decipher the genomic basis of complex traits, optimize crop genomics, and enable plant-based production of recombinant proteins. However, established plant transformation approaches for...
Article
Metabolic rates potentially regulate the pace of important physiological and life-history traits. Natural selection has shaped the evolution of metabolic rates and the physiology that supports them, including digestibility and the rate of food consumption. Understanding the relationship between metabolic rates and energy internalization is central...
Article
Full-text available
This perspective paper explores the utilization of abiotic stress-responsive transcription factors (TFs) from crassulacean acid metabolism (CAM) plants to improve abiotic stress tolerance in crop plants. CAM is a specialized type of photosynthetic adaptation that enhances water-use efficiency (WUE) by shifting CO2 uptake to all or part of the night...
Conference Paper
Crassulacean acid metabolism (CAM) plants are considered well adapted to water-limited environments because CAM enhances water-use efficiency (WUE) compared to C 3 photosynthetic plants. Still, the underlying regulatory basis of CAM is essentially unknown. We have identified several candidate transcription factors (TFs) as potential key regulators...
Article
Flight muscle breakdown has been reported for many orders of insects, but the basis of this breakdown in insects with lifelong dependence on flight is less clear. Lepidopterans show such muscle changes across their lifespans, yet how this change affects the ability of these insects to complete their life cycles is not well documented. We investigat...
Article
Mitochondrial haplotypes have been associated with human and rodent phenotypes, including nonshivering thermogenesis capacity, learning capability, and disease risk. Although the mammalian mitochondrial D-loop is highly polymorphic, D-loops in laboratory mice are identical, and variation occurs elsewhere mainly between nucleotides 9820 and 9830. Pa...
Article
Full-text available
Biological aging profoundly impairs muscle function, performance, and metabolism. Because the progression of metabolic alterations associated with aging muscle has not been chronicled, we tracked the metabolic profiles of flight muscle from middle to advanced age in Manduca sexta to identify key molecules during the progression of muscle aging, as...
Conference Paper
Vegetative desiccation tolerance, lost in most angiosperms during evolution, is an important trait in many bryophytes, and allows some plants to survive the loss of almost all cellular water and resume growth upon rehydration. Among the mosses, some are fully desiccation tolerant (DT) with constitutive mechanisms that allow them to survive drying,...
Article
Full-text available
This study explores the possible socio-cultural factors affecting addictive behaviors leading to disability in work-aged populations. A sparse generalized canonical correlation analysis was used to model and correlate socio-cultural factors with high addictive behaviors (drug, tobacco, alcohol, and food over-consumption) in 15 to 49 year olds that...
Conference Paper
Full-text available
This study explores statistically the possible socio-cultural factors impacting addictive behaviors leading to disability in work-aged populations. A sparse canonical correlation analysis was employed to explore factors that may impact work place by being correlated with high addictive behaviors related to drug, tobacco, alcohol, and food over-cons...
Article
Full-text available
Background Understanding the response of resurrection angiosperms to dehydration and rehydration is critical for deciphering the mechanisms of how plants cope with the rigors of water loss from their vegetative tissues. We have focused our studies on the C4 resurrection grass, Sporobolus stapfianus Gandoger, as a member of a group of important fora...
Article
Full-text available
Investigations into relationships between life-history traits, such as growth rate and energy metabolism, typically focus on basal metabolic rate (BMR). In contrast, investigators rarely examine maximal metabolic rate (MMR) as a relevant metric of energy metabolism, even though it indicates the maximal capacity to metabolize energy aerobically, and...
Article
Full-text available
Crassulacean acid metabolism (CAM) is a specialized mode of photosynthesis that features nocturnal CO2 uptake, facilitates increased water-use efficiency (WUE), and enables CAM plants to inhabit water-limited environments such as semi-arid deserts or seasonally dry forests. Human population growth and global climate change now present challenges fo...
Article
Full-text available
Metabolic rates are correlated with many aspects of ecology, but how selection on different aspects of metabolic rates affects their mutual evolution is poorly understood. Using laboratory mice, we artificially selected for high maximal mass-independent metabolic rate (MMR) without direct selection on mass-independent basal metabolic rate (BMR). Th...
Conference Paper
Crassulacean acid metabolism (CAM) is an elaboration of C3 photosynthesis wherein carbon assimilation occurs at night to reduce daytime water losses through a temporal separation of primary C4 and secondary C3 carboxylation reactions. The circadian clock controls the temporal separation of these potentially competing reactions. However, the underly...
Conference Paper
Crassulacean acid metabolism (CAM) is a water-use efficient photosynthetic adaptation found in more than 6% of vascular plant species. This adaptation is distinguished by the temporal separation of primary C4 and secondary C3 carboxylation reactions to reduce daytime water loss. To investigate the underlying orchestration of transcriptional network...
Conference Paper
Crassulacean acid metabolism (CAM) is an elaboration of C3 photosynthetic that limits atmospheric CO2 fixation to all or part of the nighttime thereby reducing transpiration during the day and improving water-use efficiency. The common or crystalline ice plant (Mesembryanthemum crystallinum L.) is a facultative CAM and halophytic model species that...
Conference Paper
Improved crop water-use efficiency (WUE) is critical for the long-term sustainability of agricultural production systems in the face of predicted future warmer and drier climates. Crassulacean acid metabolism (CAM) is a specialized mode of photosynthesis that enhances WUE through an inverse day/night pattern of stomatal closure/opening and improves...
Article
Wildfire has been shown to increase the short-term (1-3 yr) mobilization of mineral N and P in forest ecosystems of the Sierra Nevada Mountains and Lake Tahoe Basin. The ensuing effects on tributary and lake water quality are uncertain. The purpose of this investigation was to assess the impacts on runoff water quality over an intermediate time fra...
Article
Aerobic metabolism of vertebrates is linked to membrane fatty acid (FA) composition. Although the membrane pacemaker hypothesis posits that desaturation of FAs accounts for variation in resting or basal metabolic rate (BMR), little is known about the FA profiles that underpin variation in maximal metabolic rate (MMR). We examined membrane FA compos...
Article
Both appropriate metabolic rates and sufficient immune function are essential for survival. Consequently, eco-immunologists have hypothesized that animals may experience trade-offs between metabolic rates and immune function. Previous work has focused on how basal metabolic rate (BMR) may trade-off with immune function, but maximal metabolic rate (...
Article
Both appropriate metabolic rates and sufficient immune function are essential for survival. Consequently, eco-immunologists have hypothesized that animals may experience trade-offs between metabolic rates and immune function. Previous work has focused on how basal metabolic rate (BMR) may trade-off with immune function, but maximal metabolic rate (...
Article
Full-text available
Selaginella lepidophylla is one of only a few species of spike mosses (Selaginellaceae) that have evolved desiccation tolerance (DT) or the ability to 'resurrect' from an air-dried state. In order to understand the metabolic basis of DT, S. lepidophylla was subjected to a five-stage, rehydration/dehydration cycle, then analyzed using non-biased, gl...
Article
Full-text available
Spike mosses (Selaginellaceae) represent an ancient lineage of vascular plants in which some species have evolved desiccation tolerance (DT). A sister-group contrast to reveal the metabolic basis of DT was conducted between a desiccation-tolerant species, Selaginella lepidophylla, and a desiccation-sensitive species, Selaginella moellendorffii, at...
Article
Maximal aerobic metabolic rate (MMR) is an important physiological and ecological variable that sets an upper limit to sustained, vigorous activity. How the oxygen cascade from the external environment to the mitochondria may affect MMR has been the subject of much interest, but little is known about the metabolic profiles that underpin variation i...
Article
Full-text available
Understanding how plants tolerate dehydration is a prerequisite for developing novel strategies for improving drought tolerance. The desiccation-tolerant (DT) Sporobolus stapfianus and the desiccation-sensitive (DS) Sporobolus pyramidalis formed a sister group contrast to reveal adaptive metabolic responses to dehydration using untargeted global me...
Article
Full-text available
The genetic variances and covariances of traits must be known to predict how they may respond to selection and how covariances among them might affect their evolutionary trajectories. We used the animal model to estimate the genetic variances and covariances of basal metabolic rate (BMR) and maximal metabolic rate (MMR) in a genetically heterogeneo...
Article
Sixty-nine telemetered Phrynosoma mcallii from the Colorado Desert in California were relocated for up to nine months to examine activity patterns, home-range sizes and overlap, distance traveled between relocations, and onset and duration of hibernation. Total area used by the lizards ranged from 651–59,237 m2. Males had significantly larger mean...
Article
The habitat of the flat-tailed horned lizard (Phrynosoma mcallii) is usually described as desert flats with fine, aeolian sand, at the edges of or away from dunes. At Ocotillo Wells State Vehicle Recreation Area the highest densities of P mcallii were correlated with sparsely vegetated gravelly habitats and mud hills. These findings suggest that ei...

Projects

Projects (3)
Project
The over-arching goal is to define at the molecular level all of the genes and associated proteins required for Kalanchoë to achieve the water use efficient (WUE) adaptation of photosynthetic carbon dioxide fixation known as Crassulacean acid metabolism (CAM). To achieve this, we are decoding genomes and transcriptomes of a diverse range of Kalanchoë species to discover candidate genes required for CAM to function efficiently and we are generating transgenic RNAi and over-expression lines targeting each candidate CAM and circadian control gene in order to understand the importance of each gene to the efficient operation of the highly water-use efficient photosynthetic adaptation. Long-term, our research aims to provide the molecular genetic parts-list or blueprint for CAM that will allow further improvement of existing CAM crops such as Agave and Opuntia and also the engineering of novel crop varieties that are able to leverage the WUE CAM adaptation of photosynthesis during times of drought stress.