
Benoit CudennecUniversity of Lille · UMRt N°1158 BioEcoAgro
Benoit Cudennec
Professor
About
80
Publications
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Introduction
I am a Professor at Lille University, where I teach biochemistry, physiology, and biotechnology. My research is conducted within the team 'Health Benefits of Protein Hydrolysates and Agro-Food Co-Products: Production Control, Characterization, and Valorization' at UMRT BioEcoAgro 1158. My primary focus is on understanding the physiological processes initiated at the intestinal level following dietary protein digestion, which contribute to beneficial health effects.
Publications
Publications (80)
Expression of concern for ‘Particle-based photodynamic therapy based on indocyanine green modified plasmonic nanostructures for inactivation of a Crohn's disease-associated Escherichia coli strain’ by Roxana Jijie et al. , J. Mater. Chem. B , 2016, 4 , 2598–2605, https://doi.org/10.1039/C5TB02697K.
In response to nutrients, intestinal L‐ and K‐cells naturally secrete glucagon‐like peptide 1 (GLP‐1). GLP‐1 regulates postprandial blood glucose by increasing insulin secretion, slowing down gastric emptying and inducing satiety. A selection of specifically developed collagen hydrolysates was screened for their ability to enhance natural GLP‐1 pro...
Calcium is the most abundant mineral in the human body and is involved in critical physiological and cellular processes. It is essential for the development, maintenance, and integrity of bone...
Bioinformatics software, allowing the identification of peptides by the comparison of pep- tide fragmentation spectra obtained by mass spectrometry versus targeted databases or directly by de novo sequencing, is now mandatory in peptidomics/proteomics approaches. Programming the identification software requires specifying, among other things, the m...
Bioinformatics software, allowing the identification of peptides by the comparison of peptide fragmentation spectra obtained by mass spectrometry versus targeted databases or directly by de novo sequencing, is now mandatory in peptidomics/proteomics approaches. Programming the identification software requires specifying, among other things, the mas...
Research on new strategies to regulate glucose homeostasis to prevent or manage type 2 diabetes is a critical challenge. Several studies have shown that protein-rich diets could improve glucose homeostasis. Whey protein hydrolysis allows the release of amino acids and bioactive peptides, which exert numerous well-documented bioactivities. This stud...
Objective:
The aim of this study was to analyze the protein digestibility and postprandial metabolism in rats of milk protein matrices obtained by different industrial processes.
Material and methods:
The study was conducted on Wistar rats that consumed a meal containing different 15N-labeled milk proteins. Four milk matrices were tested: native...
Mass spectrometry has become the technique of choice for the assessment of a high variety of molecules in complex food matrices. It is best suited for monitoring the evolution of digestive processes in vivo and in vitro. However, considering the variety of equipment available in different laboratories and the diversity of sample preparation methods...
ESKAPE pathogens are considered as global threats to human health. The discovery of new molecules for which these pathogens have not yet developed resistance is a high medical priority. Synthetic flavonoids are good candidates for developing new antimicrobials. Therefore, we report here the potent in vitro antibacterial activity of BrCl-flav, a rep...
Dipeptidyl-peptidase IV (DPP-IV) plays an essential role in glucose metabolism by inactivating incretins. In this context, food-protein-derived DPP-IV inhibitors are promising glycemic regulators which may act by preventing the onset of type 2 diabetes in personalized nutrition. In this study, the DPP-IV-inhibitory potential of seven proteins from...
Today, plant production is increasing, but most industrial processes generate a lot of waste and by-products for which, in the current context, it is a priority to recycle or valorize them. One of the cheapest valorization routes is fermentation, in particular lactic fermentation by Lactobacillus species, which produces lactic acid and other molecu...
In this study, we investigate the interactions between the leaderless class IIb bacteriocin, enterocin DD14 (EntDD14), or the methicillin or the combination of these antibacterials, and two methicillin-resistant Staphylococcus aureus strains (MRSA-S1 and USA 300) which are respectively a clinical strain and a reference strain. The results obtained...
Milk and dairy products are significant sources of proteins and peptides impacting human health. In this way, the interest in CPPs, bioactive phosphorylated peptides resulting from the hydrolysis of caseins, has grown in the past years. CPPs were mainly studied for their capacity to chelate and increase the bioavailability of essential minerals inv...
Industrial chicory has been the subject of numerous studies, most of which provide clinical observations on its health effects. Whether it is the roasted root, the flour obtained from the roots or the different classes of molecules that enter into the composition of this plant, understanding the molecular mechanisms of action on the human organism...
Several studies have demonstrated that high protein diets improve glucose homeostasis. Nevertheless, the mechanisms underlying this effect remain elusive. This exploratory study aims to screen and compare the acute effects of dietary proteins from different sources on intestinal glucose absorption. Six dietary proteins from various sources were thu...
Bacteriocins have been steadily reported as potential agents that may contribute, in different ways, to overcome antimicrobial drug resistance. Here, holoxenic NMRI-F mice microbiota, their body weight recovery and histopathological alterations of organs like colon, spleen and liver were examined in mice intraperitoneally infected with 10 ⁸ cfu of...
Breast milk is the gold standard in neonatal nutrition, but most infants are fed infant formulas in which lipids are usually of plant origin. The addition of dairy lipids and/or milk fat globule membrane extracts in formulas improves their composition with beneficial consequences on protein and lipid digestion. The probiotic Lactobacillus fermentum...
Since alterations of the gut microbiota have been shown to play a major role in obesity, probiotics have attracted attention. Our aim was to identify probiotic candidates for the management of obesity using a combination of in vitro and in vivo approaches. We evaluated in vitro the ability of 23 strains to limit lipid accumulation in adipocytes and...
Like their owners, dogs and cats are more and more affected by overweight and obesity-related problems and interest in functional pet foods is growing sharply. Through numerous studies, fish protein hydrolysates have proved their worth to prevent and manage obesity-related comorbidities like diabetes. In this work, a human in vitro static simulated...
The gastrointestinal digestion of food proteins can generate peptides with a wide range of biological activities. In this study, we screened various potential bioactivities generated by plant-based proteins. Whey protein as an animal protein reference, five grades of pea protein, two grades of wheat protein, and potato, fava bean, and oat proteins...
Industrial chicory roots (Cichorium intybus var. sativum) have been the subject of many different studies investigating their supposed nutritional and medicinal benefits. Although an impressive number of clinical evidences has supported the health effects of this plant, the molecular mechanisms remain poorly understood. In this work, we looked at c...
The goal of this study was to determine the impact of industrial processes on the digestion of six milk protein matrices using the harmonized INFOGEST in vitro static digestion protocol. First, this method was optimized to simple protein matrices using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and size exclusion chromatog...
Production of bioactive peptides (BAPs) by Lactobacillus species is a cost-effective approach compared to the use of purified enzymes. In this study, proteolytic Lactobacillus helveticus strains were used for milk fermentation to produce BAPs capable of inhibiting angiotensin converting enzyme (ACE). Fermented milks were produced in bioreactors usi...
Introduction
The gastrointestinal digestion of food proteins can generate peptides having a wide range of biological activities and possibly involved in many physiological processes. In this study, we screened various potential bioactivities generated by plant-based protein samples as compared to one animal-based protein after in vitro simulation o...
Bovine whey protein concentrate (WPC) was hydrolysed under pH-stat (ST) and non pH-controlled (free-fall, FF) conditions using Debitrase (DBT) and FlavorPro Whey (FPW). The resultant whey protein hydrolysates (WPHs) were assessed for the impact of hydrolysis conditions on the physicochemical and the in vitro antioxidant and intracellular reactive o...
The identification of non-fermentative Gram negative bacilli from run-off and spring water, including fluorescent Pseudomonas is very complex and investigations are needed to contribute to the systematic of these bacteria. In this dataset, the phenotypical profiles of three strains isolated from Vosges mountains first identified as Pseudomonas fluo...
The aim of this study was to investigate the probiotic properties of 174 Lactobacillus strains isolated from Mongolian dairy products, and particularly their impact on intestinal calcium uptake and absorption. All isolates underwent a first screening based on cell surface hydrophobicity, acid tolerance, tolerance to gastro-intestinal digestion, aut...
Little is known about fluorescent Pseudomonas and investigations are needed to help us better understand how their species work. The aim was here to mimic what naturally occurs in environmental water containing strains isolated from mid-mountain water samples and identified as Pseudomonas fluorescens by conventional biochemical techniques. Three st...
Here we show that Bacillus pumilus ICVB403 recently isolated from copepod eggs is able to produce, after 48-72h of growth in Landy medium, extracellular inhibitory compounds, which are active against Staphylococcus aureus ATCC 25923, methicillin-resistant S. aureus (MRSA) ATCC 43300, MRSA-S1, Staphylococcus epidermidis 11EMB, Staphylococcus warneri...
Lactobacillus reuteri ICVB395, L. gasseri ICVB392 and L. gasseri ICVB396 strains, isolated from vaginal microbiota, were investigated for their probiotic traits. L. reuteri ICVB395 strain and the 3RG consortium, associating these three Lactobacillus strains, showed the best anti-inflammatory profile on peripheral blood mononuclear cell (PBMC) while...
To compensate for their amino acid auxotrophy, lactobacilli have developed the ability to hydrolyze proteins present in their environment. This proteolytic activity not only generates the free amino acids needed by the bacteria, but also a large variety of peptides, some of which are endowed with biological activities. These so-called “bioactive pe...
The aim of this study was to investigate the probiotic properties of 174 Lactobacillus strains isolated from
Mongolian dairy products, and particularly their impact on intestinal calcium uptake and absorption. All isolates
underwent a first screening based on cell surface hydrophobicity, acid tolerance, tolerance to gastro-intestinal
digestion, aut...
To compensate for their amino acid auxotrophy, lactobacilli have developed the ability to hydrolyze proteins present in their environment. This proteolytic activity not only generates the free amino acids needed by the bacteria, but also a large variety of peptides, some of which are endowed with biological activities. These so-called "bioactive pe...
This study aimed at comparing the genome of Enterococcus faecalis B3A-B3B, a bacteriocinogenic strain recently isolated from a healthy Iraqi infant to those of Enterococci of clinical and beneficial grades. The putative genes gelE, cpd, efaAfm, ccf, agg, and cob coding for virulence factors were detected in B3A-B3B strain, which meanwhile resulted...
The objectives of this work are to address the prebiotic effects of the chicory (Cichorium intybus) together with its possible role in appetite control. We compared nine chicory genotypes in order to determine if variations in the content of metabolites in the roasted roots would lead to modifications in release of satiety hormones and in compositi...
This study presents a miniaturized 3-methyl-2-benzothiazolinone hydrazone hydrochloride-assay (MBTH-assay)¹, optimized for high-throughput measurement of shrimp phenol oxidase activities and the screening of potential inhibitors of post-mortem melanosis. To validate this approach, assay parameters, including limit of detection and dynamic range, we...
A qualitative study is presented, where the main question was whether food-derived hemorphins, i.e., originating from digested alimentary hemoglobin, could pass the intestinal barrier and/or the blood–brain barrier (BBB). Once absorbed, hemorphins are opioid receptor (OR) ligands that may interact with peripheral and central OR and have effects on...
Copepods represent a major source of food for many aquatic species of commercial interest for aquaculture such as mysis shrimp and early stages of fishes. For the purpose of this study, the culturable mesophilic bacterial flora colonizing Acartia tonsa copepod eggs was isolated and identified. A total of 175 isolates were characterized based on the...
Food-derived hemorphins were studied for their interactions with intestinal cells in relation to food intake regulations. LLVV-H4, LVV-H4, VV-H4, VV-H7 and H7 were identified in the 2 h-intestinal hydrolysate of haemoglobin simulated gastrointestinal digestion and synthesised. These food-derived hemorphins showed secretagogue properties of the anor...
The gut plays a central role in energy homeostasis. Food intake regulation strongly relies on the gut–brain axis, and numerous studies have pointed out the significant role played by gut hormones released from enteroendocrine cells. It is well known that digestive products of dietary protein possess a high satiating effect compared to carbohydrates...
Enterococcus faecalis 14, a strain previously isolated from meconium, displayed activity against four Clostridium perfringens isolates when co-cultured on agar plates. The anti-Clostridium activity was ascribed to the production of enterocin DD14, which was subsequently purified. The minimum inhibitory concentration (MIC) of enterocin DD14 against...
Sixty-seven (67) lactic acid bacteria (LAB) isolates belonging to Lactobacillus genus were isolated from human feces and tested for their auto-aggregation and cell surface hydrophobicity in order to establish their adhesion capabilities, a prerequisite for probiotic selection. Strains with the upmost auto-aggregation and cell surface hydrophobicity...
Food intake regulation relies on appetite modulation which lies on the brain-gut axis. Short and long-term signals are sent from stomach, upper and lower intestine in response to nutrients activating neuronal, hormonal and metabolic pathways. Dietary proteins already gave evidence to generate a strong satiety feeling when entering the GI tract by s...
Lactobacilli strains with probiotic traits were tested for their ability to survive to the digestion
process modelled during this study.These strains managed to sustain the harsh conditions
of the gastric and duodenal phases and showed good adhesion capacities to human Caco-2
cell line. These probiotic microorganisms have survived during these step...
The antihypertensive potential of a land snail by-product hydrolysate (SBH), obtained after
an industrial treatment of the raw material, was studied in vitro and in vivo. The ACE inhibitory
activity of SBH was characterised by an IC50 value of 23 �g·mL−1, which was not affected
by in vitro digestion. SBH enhanced the Caco-2 intestinal cell metaboli...
Particle based photodynamic therapy (PPDT) holds great promise in disease theranostic applications. Herein, we demonstrate that PPDT-based on gold nanorods enrobed in an indocyanine green (ICG)-loaded silica shell allow for the inactivation of Crohn's disease‐associated adherent‐invasive Escherichia coli strain LF82 (E. coli LF82) under pulsed lase...
Consumers and governments have become aware how the daily diet may affect the human health. All proteins from both plant and animal origins are potential sources of a wide range of bioactive peptides and the large majority of those display health-promoting effects. In the meat production food chain, the slaughterhouse blood is an inevitable co-prod...
This study aimed at showing the yeast diversity in feces of Algerian infants, aged between 1 and 24 months, hospitalized at Bejaia hospital (north east side of the country). Thus 20 colonies with yeast characteristics were isolated and identified using biochemical (ID32C Api system), and molecular (sequencing of ITS1-5.8S-ITS2 region) methods. Almo...
In shrimp, the development of post-mortem melanosis resulting from phenoloxidase activities leads to important economic losses. Phenoloxidase enzymes include catechol oxidases, laccases and tyrosinases, but hemocyanin is also capable of phenoloxidase activities. These activities have been explored in Penaeus monodon, using different substrates. Res...
We studied here the yeast content of poultry feces, collected randomly from a French farm located in the north of the country. Thus, 81 yeast colonies were isolated and clustered into 22 distinct groups using the rep-PCR method. A single colony was taken from each group and identified using biochemical (ID 32C system) and molecular (sequencing of t...
The aim of the present study is to investigate how peptides released by gastrointestinal (GI) digestion of one dietary protein can interact with regulating processes of food intake. An in vitro GI digestion of bovine haemoglobin was carried out and the bioactivity of the digests on CCK and GLP-1 secretion and dipeptidyl peptidase IV (DPP-IV) activi...
We studied here the yeast content of poultry feces, collected randomly from a French farm located in the north of the country. Thus, 81 yeast colonies were isolated and clustered into 22 distinct groups using the rep-PCR method. A single colony was taken from each group and identified using biochemical (ID 32C system) and molecular (sequencing of t...
Two cuttlefish (Sepia officinalis) viscera protein hydrolysates were obtained with different enzymes extracted from cuttlefish and smooth hound (Mustellus mustellus). Their ability to stimulate the secretion of cholecystokinin (CCK) and glucagon-like peptide 1 (GLP-1), using the enteroendocrine STC-1 cell line, and to inhibit the DPP-IV activity du...
Le développement du phénomène de mélanose post-mortem, observé sur les crustacés et particulièrement sur les crevettes, entraîne d’importantes pertes économiques. Afin de lutter efficacement contre ce phénomène résultant d’activités phénoloxydases, il est essentiel de mieux connaître et comprendre ces activités enzymatiques. Le terme de phénoloxyda...
Gastrointestinal digestion and energetic metabolism: how generated peptides may impact intestinal hormones?
J. Caron(1), Y. Belguesmia(1), D. Domenger(1), C. Flahaut(2), M. Kouach(3), J.F. Goossens(3) P. Dhulster(1), R. Ravallec(1) and B. Cudennec(1)
1-Charles Viollette Institute,Université Lille1, Villeneuve d’Ascq, France;
2-Charles Viollette I...
The ability of Paenibacillus polymyxa to inhibit the growth of Escherichia coli generic ATCC 25922 (Escherichia coli ATCC 25922) and to adhere to monolayers of the enterocyte-like human cell line Caco-2 was evaluated. P. polymyxa JB-0501 (P. polymyxa JB-0501), found in a livestock feed probiotic supplement, was compared to P. polymyxa reference str...
The decline of European abalone Haliotis tuberculata populations has been associated with various pathogens including bacteria of the genus Vibrio. Following the summer mortality outbreaks reported in France between 1998 and 2000, Vibrio harveyi strains were isolated from moribund abalones, allowing in vivo and in vitro studies on the interactions...
Bioactive peptides obtained from marine species and marine by-products are showing these days a great interest in nutraceutical and pharmaceutical applications due to their wide range of biological activities. This mini-review focuses on the potential of marine peptides to be used as appetite suppressive molecules in the prevention and/or in the tr...
For many years, numerous kinds of processes based on enzymatic hydrolysis at various pH levels, involving added plant or bacterial enzymes and after inactivation by heating of endogenous enzymes present in the raw material, or alternatively based on the action of endogenous enzymes, have contributed to the degradation of marine by-products proteins...